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The use of oral antimicrobials in livestock, particularly in pigs, has been identified as a driver in the selection of AMR bacteria. The aim of the present study was to evaluate the effects of a single intramuscular (IM) dose of marbofloxacin (8\u00a0mg\/kg) on <jats:italic>Enterobacteriaceae<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> populations, as well as on fluoroquinolone resistance within the fecal microbiota of pigs. Twenty healthy pigs, 60-days old, were divided into two groups: a treated group (<jats:italic>n<\/jats:italic>\u2009=\u200913) and a control group (<jats:italic>n<\/jats:italic>\u2009=\u20097) and were monitored over a 28-day experimental period. Fecal samples were collected from all animals for the isolation of <jats:italic>E. coli<\/jats:italic> and <jats:italic>Salmonella<\/jats:italic> strains. The minimum inhibitory concentration (MIC) of marbofloxacin for the isolates recovered on MacConkey agar supplemented with 1 or 4\u00a0\u00b5g\/mL of marbofloxacin and for some generic <jats:italic>E. coli<\/jats:italic> isolates (recovered from MacConkey agar not supplemented with marbofloxacin) was determined using the broth microdilution method. Genomic DNA was extracted from the confirmed bacterial strains and sequenced using the Sanger method to identify mutations in the quinolone resistance determining regions (QRDRs) of the <jats:italic>gyrA<\/jats:italic> and <jats:italic>parC<\/jats:italic> genes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The single IM administration of marbofloxacin resulted in a significant decrease in <jats:italic>Enterobacteriaceae<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> fecal populations from days 1 to 3 post- treatment. No <jats:italic>Salmonella<\/jats:italic> isolates were detected in either group, and no marbofloxacin-resistant <jats:italic>E. coli<\/jats:italic> isolates were identified. The MIC of the selected generic <jats:italic>E. coli<\/jats:italic> strains (<jats:italic>n<\/jats:italic>\u2009=\u2009100) showed an increase to up to 0.5\u00a0\u00b5g\/mL between days 1 and 3 post-treatment but remained below the clinical breakpoint of marbofloxacin resistance (4\u00a0\u00b5g\/mL). Sequencing of these isolates revealed no mutations in <jats:italic>gyrA<\/jats:italic> and <jats:italic>parC<\/jats:italic> genes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The present study showed that this dosing regimen of marbofloxacin significantly decreases the fecal shedding of <jats:italic>Enterobacteriaceae<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> populations in pigs, while limiting the selection of marbofloxacin-resistant <jats:italic>E. coli<\/jats:italic> isolates. These findings warrant validation in sick pigs to support the selective use of this antibiotic solely in cases of clinical disease, thereby minimizing the reliance on conventional (metaphylactic) group treatments in pigs.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12917-024-04329-9","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T06:58:52Z","timestamp":1730185132000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of single parenteral administration of marbofloxacin on bacterial load and selection of resistant Enterobacteriaceae in the fecal microbiota of healthy pigs"],"prefix":"10.1186","volume":"20","author":[{"given":"Micaela","family":"Miyauchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farid","family":"EL Garch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Th\u00e9riault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno G.","family":"Leclerc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"L\u00e9pine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Giboin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Rhouma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,10,29]]},"reference":[{"issue":"2","key":"4329_CR1","doi-asserted-by":"publisher","first-page":"106713","DOI":"10.1016\/j.ijantimicag.2023.106713","volume":"61","author":"M Rhouma","year":"2023","unstructured":"Rhouma M, Madec J-Y, Laxminarayan R, Colistin. 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