{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T03:49:08Z","timestamp":1772941748757,"version":"3.50.1"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"DOI":"10.1186\/s12917-024-04354-8","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T02:16:25Z","timestamp":1731636985000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Stability of closed and needle-punctured vials of Porvac\u00ae subunit vaccine against classical swine fever subjected to thermal stress"],"prefix":"10.1186","volume":"20","author":[{"given":"Tal\u00eda","family":"Sardina-Gonz\u00e1lez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milagros","family":"Vargas-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusmel","family":"Sordo-Puga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Naranjo-Vald\u00e9z","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Pilar","family":"Rodr\u00edguez-Molt\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Karla","family":"M\u00e9ndez-Orta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mara Laura","family":"Hern\u00e1ndez-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine","family":"Santana-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Pena-Guimaraes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Moreira-Rubio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosaili","family":"Mateu-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ania","family":"Cabrales-Rico","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos A.","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny","family":"P\u00e9rez-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisela","family":"Su\u00e1rez-Pedroso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,15]]},"reference":[{"key":"4354_CR1","unstructured":"Manual of Diagnostic Tests and Vaccines for Terrestrial, Animals. Chap 3.9.3. Classical swine fever (infection with classical swine fever virus). 2022. https:\/\/www.woah.org\/fileadmin\/Home\/eng\/Health_standards\/tahm\/3.09.03_CSF.pdf."},{"key":"4354_CR2","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1111\/tbed.12676","volume":"65","author":"A Postel","year":"2018","unstructured":"Postel A, Austermann-Busch S, Petrov A, Moennig V, Becher P. Epidemiology, diagnosis and control of classical swine fever: Recent developments and future challenges. Transbound Emerg Dis. 2018;65:248\u201361.","journal-title":"Transbound Emerg Dis"},{"issue":"1","key":"4354_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12917-018-1504-2","volume":"14","author":"H Zhang","year":"2018","unstructured":"Zhang H, Leng C, Tian Z, Liu C, Chen J, Bai Y, Li Z, Xiang L, Zhai H, Wang Q. Complete genomic characteristics and pathogenic analysis of the newly emerged classical swine fever virus in China. BMC Vet Res. 2018;14(1):1\u201311.","journal-title":"BMC Vet Res"},{"issue":"2","key":"4354_CR4","doi-asserted-by":"publisher","first-page":"154","DOI":"10.3390\/vaccines9020154","volume":"9","author":"L Coronado","year":"2021","unstructured":"Coronado L, Perera CL, Rios L, Fr\u00edas MT, P\u00e9rez LJ. A Critical Review about Different Vaccines against Classical Swine Fever Virus and Their Repercussions in Endemic Regions. Vaccines. 2021;9(2):154.","journal-title":"Vaccines"},{"key":"4354_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.virusres.2013.10.025","volume":"179","author":"Y-L Huang","year":"2014","unstructured":"Huang Y-L, Deng M-C, Wang F-I, Huang C-C, Chang C-Y. The challenges of classical swine fever control: modified live and E2 subunit vaccines. Virus Res. 2014;179:1\u201311.","journal-title":"Virus Res"},{"key":"4354_CR6","doi-asserted-by":"publisher","first-page":"108403","DOI":"10.1016\/j.vetmic.2019.108403","volume":"237","author":"W Gong","year":"2019","unstructured":"Gong W, Li J, Wang Z, Sun J, Mi S, Xu J, Cao J, Hou Y, Wang D, Huo X. Commercial E2 subunit vaccine provides full protection to pigs against lethal challenge with 4 strains of classical swine fever virus genotype 2. Vet Microbiol. 2019;237:108403.","journal-title":"Vet Microbiol"},{"issue":"30","key":"4354_CR7","doi-asserted-by":"publisher","first-page":"5665","DOI":"10.1016\/j.vaccine.2006.12.036","volume":"25","author":"M Beer","year":"2007","unstructured":"Beer M, Reimann I, Hoffmann B, Depner K. Novel marker vaccines against classical swine fever. Vaccine. 2007;25(30):5665\u201370.","journal-title":"Vaccine"},{"issue":"8","key":"4354_CR8","doi-asserted-by":"publisher","first-page":"148","DOI":"10.3390\/vetsci8080148","volume":"8","author":"P Zhou","year":"2021","unstructured":"Zhou P, Huang J, Li Y, Chen H, Wu Y, Fu X, Hao X, Li Q, Zeng R, Zhang G. Efficiency Comparison of a Novel E2 Subunit Vaccine and a Classic C-Strain Vaccine against Classical Swine Fever. Veterinary Sci. 2021;8(8):148.","journal-title":"Veterinary Sci"},{"key":"4354_CR9","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.vetmic.2017.05.003","volume":"205","author":"S Mu\u00f1oz-Gonz\u00e1lez","year":"2017","unstructured":"Mu\u00f1oz-Gonz\u00e1lez S, Sordo Y, P\u00e9rez-Sim\u00f3 M, Suarez M, Canturri A, Rodriguez MP, Fr\u00edas-Lepoureau MT, Domingo M, Estrada MP, Ganges L. Efficacy of E2 glycoprotein fused to porcine CD154 as a novel chimeric subunit vaccine to prevent classical swine fever virus vertical transmission in pregnant sows. Vet Microbiol. 2017;205:110\u20136.","journal-title":"Vet Microbiol"},{"key":"4354_CR10","doi-asserted-by":"crossref","unstructured":"Su\u00e1rez M, Sordo Y, Prieto Y, Rodr\u00edguez M, M\u00e9ndez L, Rodr\u00edguez E, Rodr\u00edguez-Mallon A, Lorenzo E, Santana E, Gonz\u00e1lez N. A single dose of the novel chimeric subunit vaccine E2-CD154 confers early full protection against classical swine fever virus. Vaccine 2017.","DOI":"10.1016\/j.vaccine.2017.05.028"},{"key":"4354_CR11","doi-asserted-by":"publisher","first-page":"198151","DOI":"10.1016\/j.virusres.2020.198151","volume":"289","author":"L Ganges","year":"2020","unstructured":"Ganges L, Crooke HR, Boh\u00f3rquez JA, Postel A, Sakoda Y, Becher P, Ruggli N. Classical swine fever virus: The past, present and future. Virus Res. 2020;289:198151.","journal-title":"Virus Res"},{"key":"4354_CR12","doi-asserted-by":"crossref","unstructured":"Fonseca-Rodriguez O, Coronado L, Amar\u00e1n L, Perera CL, Centelles Y, Montano DN, Alfonso P, Fern\u00e1ndez O, Santoro KR, Fr\u00edas-Lepoureau MT. Descriptive epidemiology of endemic classical swine fever in cuba. Span J Agricultural Res 2018, 16(2).","DOI":"10.5424\/sjar\/2018162-12487"},{"key":"4354_CR13","doi-asserted-by":"crossref","unstructured":"Su\u00e1rez M, Sordo Y, Prieto Y, Rodr\u00edguez MP, M\u00e9ndez L, Rodr\u00edguez EM, Rodr\u00edguez-Mallon A, Lorenzo E, Santana E, Gonz\u00e1lez N. A single dose of the novel chimeric subunit vaccine E2-CD154 confers early full protection against classical swine fever virus. Vaccine 2017.","DOI":"10.1016\/j.vaccine.2017.05.028"},{"issue":"2","key":"4354_CR14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.3390\/vaccines9020167","volume":"9","author":"Y Sordo-Puga","year":"2021","unstructured":"Sordo-Puga Y, Su\u00e1rez-Pedroso M, Naranjo-Vald\u00e9z P, P\u00e9rez-P\u00e9rez D, Santana-Rodr\u00edguez E, Sardinas-Gonzalez T, Mendez-Orta MK, Duarte-Cano CA, Estrada-Garcia MP. Rodr\u00edguez-Molt\u00f3 MP: Porvac\u00ae Subunit Vaccine E2-CD154 Induces Remarkable Rapid Protection against Classical Swine Fever Virus. Vaccines. 2021;9(2):167.","journal-title":"Vaccines"},{"issue":"6","key":"4354_CR15","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1080\/14760584.2022.2053678","volume":"21","author":"R Kumar","year":"2022","unstructured":"Kumar R, Srivastava V, Baindara P, Ahmad A. Thermostable vaccines: An innovative concept in vaccine development. Expert Rev Vaccines. 2022;21(6):811\u201324.","journal-title":"Expert Rev Vaccines"},{"issue":"6","key":"4354_CR16","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1016\/j.biologicals.2009.08.017","volume":"37","author":"WHO","year":"2009","unstructured":"WHO. Guidelines on stability evaluation of vaccines. Biologicals: J Int Association Biol Stand. 2009;37(6):424\u201334.","journal-title":"Biologicals: J Int Association Biol Stand"},{"key":"4354_CR17","volume-title":"Temperature sensitivity of vaccines","author":"WHO","year":"2006","unstructured":"WHO. Temperature sensitivity of vaccines. In.: World Health Organization; 2006."},{"key":"4354_CR18","unstructured":"WOAH. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chap. 1.1.8. Principles of Veterinary Vaccine Production [https:\/\/www.woah.org\/fileadmin\/Home\/eng\/Health_standards\/tahm\/1.01.08_VACCINE_PRODUCTION.pdf]"},{"issue":"3","key":"4354_CR19","first-page":"16","volume":"9","author":"Y Sordo-Puga","year":"2022","unstructured":"Sordo-Puga Y, Sardina-Gonz\u00e1lez T, Sosa-Test\u00e9 I, Naranjo-Vald\u00e9z P, Rodr\u00edguez-Molt\u00f3 M, Santana-Rodr\u00edguez E, Vargas-Hern\u00e1ndez M, M\u00e9ndez-Orta M, P\u00e9rez-P\u00e9rez D, Oliva-C\u00e1rdenas A, et al. Shelf Life and Accelerated Stability Studies of Porvac\u00ae, a Marker Subunit Vaccine Against Classical Swine Fever. Int J Sci Res Biol Sci. 2022;9(3):16\u201324.","journal-title":"Int J Sci Res Biol Sci"},{"key":"4354_CR20","unstructured":"Fonseca O. Caracterizaci\u00f3n espaciotemporal y factores de riesgo del comportamiento end\u00e9mico de la peste porcina cl\u00e1sica en Cuba. PhD. UNAH Fructuoso Rodr\u00edguez P\u00e9rez 2016."},{"key":"4354_CR21","doi-asserted-by":"publisher","first-page":"38","DOI":"10.3389\/fvets.2020.00038","volume":"7","author":"O Fonseca-Rodr\u00edguez","year":"2020","unstructured":"Fonseca-Rodr\u00edguez O, Centelles Garcia Y, Alfonso Zamora P, Ferrer-Miranda E, Montano DN, Blanco M, Guti\u00e9rrez Y, Calistri P, Santoro KR, Percedo MI. Classical Swine Fever in a Cuban Zone Intended for Eradication: Spatiotemporal Clustering and Risk Factors. Front veterinary Sci. 2020;7:38.","journal-title":"Front veterinary Sci"},{"issue":"1\u20132","key":"4354_CR22","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.virusres.2005.03.018","volume":"112","author":"H D\u00edaz de Arce","year":"2005","unstructured":"D\u00edaz de Arce H, Ganges L, Barrera M, Naranjo D, Sobrino F, Frias MT, Nunez JI. Origin and evolution of viruses causing classical swine fever in Cuba. Virus Res. 2005;112(1\u20132):123\u201331.","journal-title":"Virus Res"},{"issue":"5","key":"4354_CR23","first-page":"1203","volume":"76","author":"S Soleimani","year":"2021","unstructured":"Soleimani S, Rashid S. Correlation Study of the Most Important Environmental Influencing Factors on the Razi MMR Vaccine. Arch Razi Inst. 2021;76(5):1203.","journal-title":"Arch Razi Inst"},{"key":"4354_CR24","doi-asserted-by":"crossref","unstructured":"Oliva-C\u00e1rdenas A, Fern\u00e1ndez-Zamora F, Santana-Rodr\u00edguez E, Sordo-Puga Y, Vargas-Hern\u00e1ndez MdlC, Rodr\u00edguez-Molt\u00f3 MP, P\u00e9rez-P\u00e9rez D, Sardina-Gonz\u00e1lez T, Duarte CA, Le\u00f3n-Go\u00f1i A. Safety and immunogenicity in piglets of two immunization schedules initiated at two or three weeks of age with Porvac\u00ae, a classical swine fever subunit marker vaccine. Bionatura 2021, 6(3).","DOI":"10.21931\/RB\/2021.06.03.3"},{"key":"4354_CR25","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-P\u00e9rez D, Sordo-Puga Y, Rodr\u00edguez-Molt\u00f3 MP, Sardina T, Santana E, Montero C, et al. E2-CD154 vaccine candidate is safe and immunogenic in pregnant sows, and the maternal derived neutralizing antibodies protect piglets from classical swine fever virus challenge. Vet Microbiol. 2021;260:109153.","DOI":"10.1016\/j.vetmic.2021.109153"},{"key":"4354_CR26","unstructured":"Su\u00e1rez-Pedroso M, Sordo-Puga Y, P\u00e9rez-P\u00e9rez D, Rodr\u00edguez-Molt\u00f3 M, Sosa-Test\u00e9 I, Oliva-C\u00e1rdenas A, Duarte C, Santana-Rodr\u00edguez E, Naranjo-Vald\u00e9z P, Gonz\u00e1lez-Fern\u00e1ndez N et al. Seguridad y eficacia a largo plazo de Porvac\u00ae, una vacuna de subunidad contra la peste porcina cl\u00e1sica. Anales de la Acad de Ciencias de Cuba 2022, 12(3)."},{"issue":"1","key":"4354_CR27","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3390\/vaccines9010007","volume":"9","author":"Y Sordo-Puga","year":"2021","unstructured":"Sordo-Puga Y, P\u00e9rez-P\u00e9rez D, Montero-Espinosa C, Oliva-C\u00e1rdenas A, Sosa-Teste I, Duarte CA, Rodr\u00edguez-Molt\u00f3 MP, Sardina-Gonz\u00e1lez T, Santana-Rodr\u00edguez E, Vargas-Hern\u00e1ndez M. Immunogenicity of E2CD154 Subunit Vaccine Candidate against Classical Swine Fever in Piglets with Different Levels of Maternally Derived Antibodies. Vaccines. 2021;9(1):7.","journal-title":"Vaccines"},{"issue":"7","key":"4354_CR28","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1080\/14789450.2016.1200469","volume":"13","author":"CP Chan","year":"2016","unstructured":"Chan CP. Forced degradation studies: current trends and future perspectives for protein-based therapeutics. Expert Rev Proteomics. 2016;13(7):651\u20138.","journal-title":"Expert Rev Proteomics"},{"issue":"11\u201312","key":"4354_CR29","first-page":"456","volume":"128","author":"V Gerdts","year":"2015","unstructured":"Gerdts V. Adjuvants for veterinary vaccines\u2013types and modes of action. Berl Munch Tierarztl Wochenschr. 2015;128(11\u201312):456\u201363.","journal-title":"Berl Munch Tierarztl Wochenschr"},{"issue":"3\u20134","key":"4354_CR30","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/j.vetmic.2009.06.027","volume":"139","author":"G-J Lin","year":"2009","unstructured":"Lin G-J, Liu T-Y, Tseng Y-Y, Chen Z-W, You C-C, Hsuan S-L, Chien M-S, Huang C. Yeast-expressed classical swine fever virus glycoprotein E2 induces a protective immune response. Vet Microbiol. 2009;139(3\u20134):369\u201374.","journal-title":"Vet Microbiol"},{"issue":"9","key":"4354_CR31","doi-asserted-by":"publisher","first-page":"e106891","DOI":"10.1371\/journal.pone.0106891","volume":"9","author":"RH Hua","year":"2014","unstructured":"Hua RH, Huo H, Li YN, Xue Y, Wang XL, Guo LP, Zhou B, Song Y, Bu ZG. Generation and efficacy evaluation of recombinant classical swine fever virus E2 glycoprotein expressed in stable transgenic mammalian cell line. PLoS ONE. 2014;9(9):e106891.","journal-title":"PLoS ONE"},{"issue":"1","key":"4354_CR32","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1186\/s12917-016-0823-4","volume":"12","author":"R Madera","year":"2016","unstructured":"Madera R, Gong W, Wang L, Burakova Y, Lleellish K, Galliher-Beckley A, Nietfeld J, Henningson J, Jia K, Li P. Pigs immunized with a novel E2 subunit vaccine are protected from subgenotype heterologous classical swine fever virus challenge. BMC Vet Res. 2016;12(1):197.","journal-title":"BMC Vet Res"},{"key":"4354_CR33","doi-asserted-by":"crossref","unstructured":"Su\u00e1rez-Pedroso M, Sordo-Puga Y, Rodr\u00edguez-Molt\u00f3 MP, Naranjo-Vald\u00e9s P, P\u00e9rez-P\u00e9rez D, Sosa-Test\u00e9 I, Montero-Espinosa C, Fuentes-Rodr\u00edguez Y, Sardina-Gonz\u00e1lez T, Santana-Rodr\u00edguez E et al. Neutralizing antibodies as a correlate of protection against classical swine fever in Porvac\u00ae vaccinated pigs. Bionatura 2023, 8(1).","DOI":"10.21931\/RB\/2023.08.01.49"},{"issue":"2","key":"4354_CR34","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/0378-1135(87)90002-2","volume":"14","author":"P Biront","year":"1987","unstructured":"Biront P, Leunen J, Vandeputte J. Inhibition of virus replication in the tonsils of pigs previously vaccinated with a Chinese strain vaccine and challenged oronasally with a virulent strain of classical swine fever virus. Vet Microbiol. 1987;14(2):105\u201313.","journal-title":"Vet Microbiol"},{"issue":"2","key":"4354_CR35","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/0378-1135(88)90036-3","volume":"16","author":"C Terpstra","year":"1988","unstructured":"Terpstra C, Wensvoort G. The protective value of vaccine-induced neutralising antibody titres in swine fever. Vet Microbiol. 1988;16(2):123\u20138.","journal-title":"Vet Microbiol"},{"issue":"2","key":"4354_CR36","doi-asserted-by":"publisher","first-page":"e56417","DOI":"10.1371\/journal.pone.0056417","volume":"8","author":"E Fern\u00e1ndez","year":"2013","unstructured":"Fern\u00e1ndez E, Toledo JR, M\u00e9ndez L, Gonz\u00e1lez N, Parra F, Mart\u00edn-Alonso JM, Limonta M, S\u00e1nchez K, Cabrales A, Estrada MP. Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine. PLoS ONE. 2013;8(2):e56417.","journal-title":"PLoS ONE"},{"issue":"1","key":"4354_CR37","first-page":"1501","volume":"35","author":"M Vargas-Hern\u00e1ndez","year":"2018","unstructured":"Vargas-Hern\u00e1ndez M, Santana-Rodr\u00edguez E, Montero-Espinosa C, Sordo-Puga Y, Acosta-Hern\u00e1ndez A, Fuentes-Rodr\u00edguez Y, P\u00e9rez-P\u00e9rez D, Oliva-C\u00e1rdenas A, Gonz\u00e1lez-Ramos E, Duarte CA. Stability, safety and protective immunity of Gavac\u00ae vaccine subjected to heat stress. Biotecnolog\u00eda Aplicada. 2018;35(1):1501\u20133.","journal-title":"Biotecnolog\u00eda Aplicada"},{"key":"4354_CR38","doi-asserted-by":"publisher","first-page":"15","DOI":"10.3126\/nvj.v36i0.27748","volume":"36","author":"M Acharya","year":"2019","unstructured":"Acharya M, Adhikari S, Awasthi H, Jha A, Singh U. Field Verification Trial of ND I-2 Vaccine in Nepal. Nepal Veterinary J. 2019;36:15\u201322.","journal-title":"Nepal Veterinary J"},{"issue":"3\u20134","key":"4354_CR39","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.vetimm.2008.10.330","volume":"127","author":"M Barrera","year":"2009","unstructured":"Barrera M, Sanchez O, Prieto Y, Castell S, Naranjo P, Rodriguez MP, Farn\u00f3s O, Aguilera A, Frias M, Fonseca O. Thermal stress treatment does not affect the stability and protective capacity of goat milk derived E2-marker vaccine formulation against CSFV. Vet Immunol Immunopathol. 2009;127(3\u20134):325\u201331.","journal-title":"Vet Immunol Immunopathol"},{"issue":"52","key":"4354_CR40","doi-asserted-by":"publisher","first-page":"8371","DOI":"10.1016\/j.vaccine.2020.10.093","volume":"38","author":"X-x Zuo","year":"2020","unstructured":"Zuo X-x, Zhao Y-h, Zhou M-x, Deng B-h, Lv HL-g, Lu F, Hou Y. -b: Live vaccine preserved at room temperature: Preparation and characterization of a freeze-dried classical swine fever virus vaccine. Vaccine. 2020;38(52):8371\u20138.","journal-title":"Vaccine"},{"key":"4354_CR41","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.biologicals.2020.07.004","volume":"68","author":"R Pachauri","year":"2020","unstructured":"Pachauri R, Manu M, Vishnoi P, Preethi BO, Tiwari AK, Dhar P. Stability of live attenuated classical swine fever cell culture vaccine virus in liquid form for developing an oral vaccine. Biologicals. 2020;68:108\u201311.","journal-title":"Biologicals"},{"key":"4354_CR42","unstructured":"Pimentel-P\u00e9rez R, Zamora-S\u00e1nchez J, L\u00f3pez-S\u00e1ez Y, Segura-Silva RM, Olivera-Romero V, P\u00e9rez-S\u00e1nchez A, UN ADYUVANTE DE ORIGEN VEGETAL PARA EL PROCESO DE FORMULACI\u00d3N DE LA VACUNA GAVAC\u00ae. EVALUACI\u00d3N DE. Revista Cientifica de la Facultade de Veterinaria 2018, 28(6)."},{"key":"4354_CR43","unstructured":"Enr\u00edquez A, Canales M, Ramos E, Dandie H, Bou\u00e9 O, Soto A, Cabrera D. Production of a recombinant vaccine against Boophilus microplus. In.: Elfos Scientiae Havana, Cuba; 1995: 79\u2013102."},{"key":"4354_CR44","unstructured":"Sterility tests. USP 46. United States Pharmacopeia [http:\/\/online.uspnf.com\/uspnf\/document\/1_GUID-481C30EA-8A49-4A77-9E81-D0CD7C533498_1_en-US]."},{"key":"4354_CR45","unstructured":"VICH. Harmonization of criteria to waive target animal batch safety testing (TABST) for inactivated vaccines for veterinary use, GL50 2014."},{"issue":"2","key":"4354_CR46","first-page":"30","volume":"9","author":"E Santana-Rodr\u00edguez","year":"2022","unstructured":"Santana-Rodr\u00edguez E, M\u00e9ndez-Orta M, Sardina-Gonz\u00e1lez T, Rodr\u00edguez-Molt\u00f3 M, Castell-Brizuela S, Sordo-Puga Y, P\u00e9rez-P\u00e9rez D, Fundora-Llera A, Oliva-C\u00e1rdenas A, Vargas-Hern\u00e1ndez M, et al. Consistency of the Neutralizing Peroxidase Linked Assay for Classical Swine Fever and Homologation with an OIE Reference Laboratory. Int J Sci Res Biol Sci. 2022;9(2):30\u20134.","journal-title":"Int J Sci Res Biol Sci"},{"key":"4354_CR47","unstructured":"VICH. Target Animal Safety for Veterinary live and inactivated Vaccines. GL44. In.; 2010."}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-024-04354-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-024-04354-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-024-04354-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T03:05:30Z","timestamp":1731639930000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-024-04354-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,15]]},"references-count":47,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["4354"],"URL":"https:\/\/doi.org\/10.1186\/s12917-024-04354-8","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4003547\/v1","asserted-by":"object"}]},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,15]]},"assertion":[{"value":"1 March 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experiments were approved and conducted following the guidelines of the Ethics Committee on Animal Experimentation (CICUAL). Approved protocols numbers VE2CD-0120\/2, VE2CD-0121\/2 and VE2CD-0220\/2. All procedures and samplings involving animals were carried out following the Guide for the Care and Use of Laboratory Animals (National Research Council, 2011).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"514"}}