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Outbreaks of human salmonellosis have often been associated with consumption of contaminated poultry products. Various strategies have been explored to control this microorganism during poultry production and processing. Vaccination of broiler chickens is regarded as one of the effectives means to control this microorganism. The aim of the present study was to compare the epitope identification in the\n                      <jats:italic>Salmonella enterica<\/jats:italic>\n                      serotype Heidelberg FlgK protein by in silico prediction and in vivo experiment with mass spectrometry in association with immunoprecipitation proteomics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>Salmonella<\/jats:italic>\n                      serotype Heidelberg FlgK protein contains 553 amino acids with a molecular mass of 61\u00a0kDa. This protein is conserved among\n                      <jats:italic>Salmonella<\/jats:italic>\n                      serotype Heidelberg isolates. The results show that both approaches identified three common shared consensus peptide epitope sequences at the positions of 77\u201395, 243\u2013255 and 358\u2013373 in the\n                      <jats:italic>Salmonella<\/jats:italic>\n                      serotype Heidelberg FlgK protein.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings provide a rational for further evaluation of these shared linear epitopes in vaccine development to cover the chicken population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12917-025-04479-4","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T07:10:01Z","timestamp":1738825801000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Epitope mapping of recombinant Salmonella enterica\u00a0serotype Heidelberg flagellar hook-associated protein by in silico and in vivo approaches"],"prefix":"10.1186","volume":"21","author":[{"given":"Hung-Yueh","family":"Yeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"4479_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106130","volume":"180","author":"MHJN Abadi","year":"2023","unstructured":"Abadi MHJN, Abyaneh FA, Zare N, Zamani J, Abdoli A, Aslanbeigi F, Hamblin MR, Tarrahimofrad H, Rahimi M, Hashemian SM, Mirzaei H. 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