{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:33:41Z","timestamp":1784043221042,"version":"3.55.0"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T00:00:00Z","timestamp":1739491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T00:00:00Z","timestamp":1739491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Yunnan Ten Thousand Talents Plan Young and Elite Talents Project","award":["YNWR-CYJS-2018-047"],"award-info":[{"award-number":["YNWR-CYJS-2018-047"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31960692"],"award-info":[{"award-number":["31960692"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"DOI":"10.1186\/s12917-025-04515-3","type":"journal-article","created":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T16:57:14Z","timestamp":1739552234000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Escherichia coli HPI-induced duodenitis through ubiquitin regulation of the TLR4\/NF-\u03baB pathway"],"prefix":"10.1186","volume":"21","author":[{"given":"Jingang","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlan","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Cha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jue","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiguo","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,14]]},"reference":[{"issue":"1","key":"4515_CR1","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.ijmm.2016.11.004","volume":"307","author":"M Smati","year":"2017","unstructured":"Smati M, Magistro G, Adiba S, Wieser A, Picard B, Schubert S, et al. Strain-specific impact of the high-pathogenicity island on virulence in extra-intestinal pathogenic Escherichia coli. Int J Med Microbiol. 2017;307(1):44\u201356.","journal-title":"Int J Med Microbiol"},{"issue":"1","key":"4515_CR2","doi-asserted-by":"publisher","first-page":"e0239121","DOI":"10.1128\/mBio.02391-21","volume":"13","author":"GL Katumba","year":"2022","unstructured":"Katumba GL, Tran H, Henderson JP. The Yersinia High-Pathogenicity Island encodes a siderophore-dependent copper response system in Uropathogenic Escherichia coli. mBio. 2022;13(1):e0239121.","journal-title":"mBio"},{"issue":"9","key":"4515_CR3","doi-asserted-by":"publisher","first-page":"5335","DOI":"10.1128\/IAI.70.9.5335-5337.2002","volume":"70","author":"S Schubert","year":"2002","unstructured":"Schubert S, Picard B, Gouriou S, Heesemann J, Denamur E. Yersinia high-pathogenicity island contributes to virulence in Escherichia coli causing extraintestinal infections. Infect Immun. 2002;70(9):5335\u20137.","journal-title":"Infect Immun"},{"issue":"2\u20133","key":"4515_CR4","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.ijmm.2004.06.026","volume":"294","author":"S Schubert","year":"2004","unstructured":"Schubert S, Rakin A, Heesemann J. The Yersinia high-pathogenicity island (HPI): evolutionary and functional aspects. Int J Med Microbiol. 2004;294(2\u20133):83\u201394.","journal-title":"Int J Med Microbiol"},{"issue":"10","key":"4515_CR5","doi-asserted-by":"publisher","first-page":"e0183950","DOI":"10.1371\/journal.pone.0183950","volume":"12","author":"G Magistro","year":"2017","unstructured":"Magistro G, Magistro C, Stief CG, Schubert S. The high-pathogenicity island (HPI) promotes flagellum-mediated motility in extraintestinal pathogenic Escherichia coli. PLoS ONE. 2017;12(10):e0183950.","journal-title":"PLoS ONE"},{"key":"4515_CR6","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.rvsc.2021.01.006","volume":"135","author":"W Zhao","year":"2021","unstructured":"Zhao W, Gao B, Liu C, Zhang B, Shan C, Deng J, et al. High pathogenicity island is associated with enhanced autophagy in pathogenic Escherichia coli HPI - infected macrophages. Res Vet Sci. 2021;135:113\u201320.","journal-title":"Res Vet Sci"},{"issue":"1","key":"4515_CR7","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1186\/s12917-021-02824-x","volume":"17","author":"C Shan","year":"2021","unstructured":"Shan C, Miao S, Liu C, Zhang B, Zhao W, Wang H, et al. Induction of macrophage pyroptosis-related factors by pathogenic E. Coli high pathogenicity island (HPI) in Yunnan Saba pigs. BMC Vet Res. 2021;17(1):114.","journal-title":"BMC Vet Res"},{"issue":"5","key":"4515_CR8","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/nri2079","volume":"7","author":"LA O\u2019Neill","year":"2007","unstructured":"O\u2019Neill LA, Bowie AG. The family of five: TIR-domain-containing adaptors in toll-like receptor signalling. Nat Rev Immunol. 2007;7(5):353\u201364.","journal-title":"Nat Rev Immunol"},{"issue":"6","key":"4515_CR9","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1016\/j.cell.2007.11.032","volume":"131","author":"LA O\u2019Neill","year":"2007","unstructured":"O\u2019Neill LA. TAMpering with toll-like receptor signaling. Cell. 2007;131(6):1039\u201341.","journal-title":"Cell"},{"issue":"11","key":"4515_CR10","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1038\/nbt1109-999","volume":"27","author":"DE Zak","year":"2009","unstructured":"Zak DE, Aderem A. A systems view of host defense. Nat Biotechnol. 2009;27(11):999\u20131001.","journal-title":"Nat Biotechnol"},{"key":"4515_CR11","doi-asserted-by":"publisher","first-page":"114291","DOI":"10.1016\/j.ejmech.2022.114291","volume":"235","author":"Y Zhang","year":"2022","unstructured":"Zhang Y, Liang X, Bao X, Xiao W, Chen G. Toll-like receptor 4 (TLR4) inhibitors: current research and prospective. Eur J Med Chem. 2022;235:114291.","journal-title":"Eur J Med Chem"},{"key":"4515_CR12","doi-asserted-by":"publisher","first-page":"111802","DOI":"10.1016\/j.intimp.2024.111802","volume":"131","author":"J Guo","year":"2024","unstructured":"Guo J, Zhang QY, Xu L, Li M, Sun QY. Icariin ameliorates LPS-induced acute lung injury in mice via complement C5a-C5aR1 and TLR4 signaling pathways. Int Immunopharmacol. 2024;131:111802.","journal-title":"Int Immunopharmacol"},{"issue":"6","key":"4515_CR13","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1038\/nri1630","volume":"5","author":"FY Liew","year":"2005","unstructured":"Liew FY, Xu D, Brint EK, O\u2019Neill LA. Negative regulation of toll-like receptor-mediated immune responses. Nat Rev Immunol. 2005;5(6):446\u201358.","journal-title":"Nat Rev Immunol"},{"key":"4515_CR14","doi-asserted-by":"publisher","first-page":"1376819","DOI":"10.3389\/fmicb.2024.1376819","volume":"15","author":"YN Guo","year":"2024","unstructured":"Guo YN, He KR, Liang SS, Mou RW, Lu MH, He YM, et al. The effect and mechanism of volatile oil emulsion from leaves of Clausena lansium (lour.) Skeels on Staphylococcus aureus in vitro. Front Microbiol. 2024;15:1376819.","journal-title":"Front Microbiol"},{"key":"4515_CR15","doi-asserted-by":"publisher","first-page":"109623","DOI":"10.1016\/j.cbi.2021.109623","volume":"348","author":"S Li","year":"2021","unstructured":"Li S, Zheng X, Hu Y, You K, Wang J. RNF31 mediated ubiquitination of A20 aggravates inflammation and hepatocyte apoptosis through the TLR4\/MyD88\/NF-\u03baB signaling pathway. Chem Biol Interact. 2021;348:109623.","journal-title":"Chem Biol Interact"},{"key":"4515_CR16","doi-asserted-by":"publisher","first-page":"110175","DOI":"10.1016\/j.intimp.2023.110175","volume":"119","author":"J Wang","year":"2023","unstructured":"Wang J, Yang J, Xia W, Zhang M, Tang H, Wang K, et al. Escherichia coli enhances Th17\/Treg imbalance via TLR4\/NF-\u03baB signaling pathway in oral lichen planus. Int Immunopharmacol. 2023;119:110175.","journal-title":"Int Immunopharmacol"},{"issue":"6","key":"4515_CR17","doi-asserted-by":"publisher","first-page":"e1000960","DOI":"10.1371\/journal.ppat.1000960","volume":"6","author":"B Wu","year":"2010","unstructured":"Wu B, Skarina T, Yee A, Jobin MC, Dileo R, Semesi A, et al. NleG type 3 effectors from enterohaemorrhagic Escherichia coli are U-Box E3 ubiquitin ligases. PLoS Pathog. 2010;6(6):e1000960.","journal-title":"PLoS Pathog"},{"key":"4515_CR18","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.rvsc.2018.08.009","volume":"120","author":"C Liu","year":"2018","unstructured":"Liu C, Shan C, Dong Q, Fu G, Zhao R, Yan Y, et al. Pathogenic E. Coli HPI upregulate the expression of inflammatory factors in porcine small intestinal epithelial cells by ubiquitin proteasome pathway. Res Vet Sci. 2018;120:41\u20136.","journal-title":"Res Vet Sci"},{"issue":"5","key":"4515_CR19","doi-asserted-by":"publisher","first-page":"101030","DOI":"10.1016\/j.psj.2021.101030","volume":"100","author":"LP Tang","year":"2021","unstructured":"Tang LP, Li WH, Liu YL, Lun JC, He YM. Heat stress aggravates intestinal inflammation through TLR4-NF-\u03baB signaling pathway in Ma chickens infected with Escherichia coli O157:H7. Poult Sci. 2021;100(5):101030.","journal-title":"Poult Sci"},{"issue":"4","key":"4515_CR20","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1016\/j.molcel.2010.10.009","volume":"40","author":"I Bosanac","year":"2010","unstructured":"Bosanac I, Wertz IE, Pan B, Yu C, Kusam S, Lam C, et al. Ubiquitin binding to A20 ZnF4 is required for modulation of NF-\u03baB signaling. Mol Cell. 2010;40(4):548\u201357.","journal-title":"Mol Cell"},{"issue":"1","key":"4515_CR21","doi-asserted-by":"publisher","first-page":"2296","DOI":"10.1038\/s41598-019-38691-8","volume":"9","author":"K Klarstr\u00f6m Engstr\u00f6m","year":"2019","unstructured":"Klarstr\u00f6m Engstr\u00f6m K, Zhang B, Demirel I. Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli. Sci Rep. 2019;9(1):2296.","journal-title":"Sci Rep"},{"issue":"4","key":"4515_CR22","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1038\/cr.2016.43","volume":"26","author":"R Hu","year":"2016","unstructured":"Hu R, Hochstrasser M. Recent progress in ubiquitin and ubiquitin-like protein (Ubl) signaling. Cell Res. 2016;26(4):389\u201390.","journal-title":"Cell Res"},{"issue":"3","key":"4515_CR23","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1093\/procel\/pwad045","volume":"15","author":"X Sheng","year":"2024","unstructured":"Sheng X, Xia Z, Yang H, Hu R. The ubiquitin codes in cellular stress responses. Protein Cell. 2024;15(3):157\u201390.","journal-title":"Protein Cell"},{"issue":"8","key":"4515_CR24","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1038\/nrm3836","volume":"15","author":"K Iwai","year":"2014","unstructured":"Iwai K, Fujita H, Sasaki Y. Linear ubiquitin chains: NF-\u03baB signalling, cell death and beyond. Nat Rev Mol Cell Biol. 2014;15(8):503\u20138.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"1","key":"4515_CR25","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1038\/cr.2010.170","volume":"21","author":"S Liu","year":"2011","unstructured":"Liu S, Chen ZJ. Expanding role of ubiquitination in NF-\u03baB signaling. Cell Res. 2011;21(1):6\u201321.","journal-title":"Cell Res"},{"issue":"8","key":"4515_CR26","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1038\/ncb0805-758","volume":"7","author":"ZJ Chen","year":"2005","unstructured":"Chen ZJ. Ubiquitin signalling in the NF-kappaB pathway. Nat Cell Biol. 2005;7(8):758\u201365.","journal-title":"Nat Cell Biol"},{"issue":"2","key":"4515_CR27","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1136\/gut.2007.125963","volume":"57","author":"T Watanabe","year":"2008","unstructured":"Watanabe T, Higuchi K, Kobata A, Nishio H, Tanigawa T, Shiba M, et al. Non-steroidal anti-inflammatory drug-induced small intestinal damage is toll-like receptor 4 dependent. Gut. 2008;57(2):181\u20137.","journal-title":"Gut"},{"issue":"2","key":"4515_CR28","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/j.ajpath.2012.10.014","volume":"182","author":"S Guo","year":"2013","unstructured":"Guo S, Al-Sadi R, Said HM, Ma TY. Lipopolysaccharide causes an increase in intestinal tight junction permeability in vitro and in vivo by inducing enterocyte membrane expression and localization of TLR-4 and CD14. Am J Pathol. 2013;182(2):375\u201387.","journal-title":"Am J Pathol"},{"key":"4515_CR29","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.jss.2019.05.036","volume":"243","author":"Q Jiang","year":"2019","unstructured":"Jiang Q, Wang S, Shi Y. Posttreatment with LYRM03 protects rats from Acute Lung inflammation Induced by Lipopolysaccharide via suppressing the NF-\u03baB\/MyD88\/TLR4 Axis. J Surg Res. 2019;243:316\u201324.","journal-title":"J Surg Res"},{"issue":"9","key":"4515_CR30","doi-asserted-by":"publisher","first-page":"2648","DOI":"10.1021\/acs.jafc.9b07611","volume":"68","author":"J Lan","year":"2020","unstructured":"Lan J, Dou X, Li J, Yang Y, Xue C, Wang C, et al. l-Arginine ameliorates Lipopolysaccharide-Induced Intestinal inflammation through inhibiting the TLR4\/NF-\u03baB and MAPK Pathways and stimulating \u03b2-Defensin expression in Vivo and in Vitro. J Agric Food Chem. 2020;68(9):2648\u201363.","journal-title":"J Agric Food Chem"},{"issue":"5","key":"4515_CR31","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.3892\/ijmm.2017.2947","volume":"39","author":"YY Li","year":"2017","unstructured":"Li YY, Zhang GY, He JP, Zhang DD, Kong XX, Yuan HM, et al. Ufm1 inhibits LPS-induced endothelial cell inflammatory responses through the NF-\u03baB signaling pathway. Int J Mol Med. 2017;39(5):1119\u201326.","journal-title":"Int J Mol Med"},{"issue":"6","key":"4515_CR32","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1093\/jb\/mvq044","volume":"147","author":"Y Kimura","year":"2010","unstructured":"Kimura Y, Tanaka K. Regulatory mechanisms involved in the control of ubiquitin homeostasis. J Biochem. 2010;147(6):793\u20138.","journal-title":"J Biochem"},{"issue":"12","key":"4515_CR33","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.1007\/s10495-006-0197-3","volume":"11","author":"NA Syed","year":"2006","unstructured":"Syed NA, Andersen PL, Warrington RC, Xiao W. Uev1A, a ubiquitin conjugating enzyme variant, inhibits stress-induced apoptosis through NF-kappaB activation. Apoptosis. 2006;11(12):2147\u201357.","journal-title":"Apoptosis"},{"issue":"3","key":"4515_CR34","doi-asserted-by":"publisher","first-page":"224","DOI":"10.23876\/j.krcp.2017.36.3.224","volume":"36","author":"J Jang","year":"2017","unstructured":"Jang J, Yoon Y, Oh DJ. A calpain inhibitor protects against fractalkine production in lipopolysaccharide-treated endothelial cells. Kidney Res Clin Pract. 2017;36(3):224\u201331.","journal-title":"Kidney Res Clin Pract"},{"issue":"7","key":"4515_CR35","doi-asserted-by":"publisher","first-page":"e13057","DOI":"10.1111\/cpr.13057","volume":"54","author":"GZ Zhang","year":"2021","unstructured":"Zhang GZ, Liu MQ, Chen HW, Wu ZL, Gao YC, Ma ZJ, et al. NF-\u03baB signalling pathways in nucleus pulposus cell function and intervertebral disc degeneration. Cell Prolif. 2021;54(7):e13057.","journal-title":"Cell Prolif"},{"issue":"1","key":"4515_CR36","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1038\/nrm2295","volume":"9","author":"I De Domenico","year":"2008","unstructured":"De Domenico I, McVey Ward D, Kaplan J. Regulation of iron acquisition and storage: consequences for iron-linked disorders. Nat Rev Mol Cell Biol. 2008;9(1):72\u201381.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"2","key":"4515_CR37","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1017\/S0007114519000928","volume":"122","author":"C Huang","year":"2019","unstructured":"Huang C, Wang Y, He X, Jiao N, Zhang X, Qiu K, et al. The involvement of NF-\u03baB\/P38 pathways in Scutellaria baicalensis extracts attenuating of Escherichia coli K88-induced acute intestinal injury in weaned piglets. Br J Nutr. 2019;122(2):152\u201361.","journal-title":"Br J Nutr"},{"issue":"5","key":"4515_CR38","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1016\/j.molcel.2012.06.017","volume":"47","author":"SJ van Wijk","year":"2012","unstructured":"van Wijk SJ, Fiskin E, Putyrski M, Pampaloni F, Hou J, Wild P, et al. Fluorescence-based sensors to monitor localization and functions of linear and K63-linked ubiquitin chains in cells. Mol Cell. 2012;47(5):797\u2013809.","journal-title":"Mol Cell"},{"issue":"5","key":"4515_CR39","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1007\/s10735-013-9498-3","volume":"44","author":"HC Liu","year":"2013","unstructured":"Liu HC, Zhao H, Chen J, Wu WL, Wang HL, Jiao GJ, et al. Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats. J Mol Histol. 2013;44(5):535\u201344.","journal-title":"J Mol Histol"},{"key":"4515_CR40","doi-asserted-by":"publisher","first-page":"411","DOI":"10.3389\/fvets.2020.00411","volume":"7","author":"PV Turner","year":"2020","unstructured":"Turner PV, Hickman DL, van Luijk J, Ritskes-Hoitinga M, Sargeant JM, Kurosawa TM, et al. Welfare Impact of Carbon Dioxide Euthanasia on Laboratory mice and rats: a systematic review. Front Vet Sci. 2020;7:411.","journal-title":"Front Vet Sci"},{"issue":"1","key":"4515_CR41","first-page":"50","volume":"51","author":"H Valentine","year":"2012","unstructured":"Valentine H, Williams WO, Maurer KJ. Sedation or inhalant anesthesia before euthanasia with CO2 does not reduce behavioral or physiologic signs of pain and stress in mice. J Am Assoc Lab Anim Sci. 2012;51(1):50\u20137.","journal-title":"J Am Assoc Lab Anim Sci"},{"issue":"6","key":"4515_CR42","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1007\/s00284-022-02873-x","volume":"79","author":"B Zhang","year":"2022","unstructured":"Zhang B, Zhao W, Gao B, Shan C, Wang X, Wan Q, et al. Whole genome sequencing and biological characteristics of two strains of Porcine Escherichia coli isolated from Saba Pigs. Curr Microbiol. 2022;79(6):182.","journal-title":"Curr Microbiol"},{"issue":"4","key":"4515_CR43","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1007\/s10616-011-9362-9","volume":"63","author":"MM Geens","year":"2011","unstructured":"Geens MM, Niewold TA. Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization. Cytotechnology. 2011;63(4):415\u201323.","journal-title":"Cytotechnology"},{"issue":"5","key":"4515_CR44","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1007\/s00284-002-3728-3","volume":"45","author":"A Tauch","year":"2002","unstructured":"Tauch A, Kirchner O, L\u00f6ffler B, G\u00f6tker S, P\u00fchler A, Kalinowski J. Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1. Curr Microbiol. 2002;45(5):362\u20137.","journal-title":"Curr Microbiol"},{"issue":"4","key":"4515_CR45","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1002\/cac2.12023","volume":"40","author":"WCC Tan","year":"2020","unstructured":"Tan WCC, Nerurkar SN, Cai HY, Ng HHM, Wu D, Wee YTF, et al. Overview of multiplex immunohistochemistry\/immunofluorescence techniques in the era of cancer immunotherapy. Cancer Commun (Lond). 2020;40(4):135\u201353.","journal-title":"Cancer Commun (Lond)"},{"issue":"1\u20132","key":"4515_CR46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cca.2007.07.003","volume":"385","author":"W Ruan","year":"2007","unstructured":"Ruan W, Lai M. Actin, a reliable marker of internal control? Clin Chim Acta. 2007;385(1\u20132):1\u20135.","journal-title":"Clin Chim Acta"},{"issue":"6","key":"4515_CR47","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"TD Schmittgen","year":"2008","unstructured":"Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3(6):1101\u20138.","journal-title":"Nat Protoc"},{"issue":"5","key":"4515_CR48","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1089\/omi.2011.0118","volume":"16","author":"G Yu","year":"2012","unstructured":"Yu G, Wang LG, Han Y, He QY. clusterProfiler: an R package for comparing biological themes among gene clusters. Omics. 2012;16(5):284\u20137.","journal-title":"Omics"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-025-04515-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-025-04515-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-025-04515-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T16:59:20Z","timestamp":1739552360000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-025-04515-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,14]]},"references-count":48,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["4515"],"URL":"https:\/\/doi.org\/10.1186\/s12917-025-04515-3","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,14]]},"assertion":[{"value":"31 July 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experiment\u2019s design was authorized by Yunnan Agriculture University\u2019s Institutional Animal Care and Use Committee (protocol code: 202303006, 3 March 2023). The animals are being grown and maintained in compliance with the requirements outlined in Yunnan Province\u2019s Rules for the Administration of Laboratory Animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"66"}}