{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T11:50:28Z","timestamp":1771847428399,"version":"3.50.1"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"DOI":"10.1186\/s12917-025-04660-9","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T05:37:57Z","timestamp":1744177077000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Can qPCR on pooled sheep milk detect brucellosis as part of herd brucellosis control and eradication programs?"],"prefix":"10.1186","volume":"21","author":[{"given":"Mohammad Javad","family":"Aminzadeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khadijeh","family":"Hashemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamideh Kalateh","family":"Rahmani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narges","family":"Khaleghnia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Azizzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pezhman","family":"Mirshokraei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,9]]},"reference":[{"key":"4660_CR1","first-page":"1","volume":"740","author":"Joint FAO\/WHO expert committee on brucellosis","year":"1986","unstructured":"Joint FAO\/WHO expert committee on brucellosis. World Health Organ Tech Rep Ser. 1986;740:1\u2013132.","journal-title":"World Health Organ Tech Rep Ser"},{"issue":"5","key":"4660_CR2","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1093\/clinids\/5.5.821","volume":"5","author":"EJ Young","year":"1983","unstructured":"Young EJ. Human Brucellosis. Rev Infect Dis. 1983;5(5):821\u201342.","journal-title":"Rev Infect Dis"},{"issue":"1","key":"4660_CR3","first-page":"357235","volume":"2012","author":"AM Behroozikhah","year":"2012","unstructured":"Behroozikhah AM, Bagheri Nejad R, Amiri K, Bahonar AR. Identification at biovar level of Brucella isolates causing abortion in small ruminants of Iran. Journal of pathogens. 2012;2012(1): 357235.","journal-title":"Journal of pathogens"},{"key":"4660_CR4","first-page":"207","volume":"2012","author":"JA Zambriski","year":"2012","unstructured":"Zambriski JA, Maves RC, Nydam DV, Ayvar V, Cepeda D, Castillo R, et al. Effect of storage temperature and sample volume on Brucella melitensis isolation from goat milk. Int J Trop Dis Health. 2012;2012:207\u201313.","journal-title":"Int J Trop Dis Health"},{"key":"4660_CR5","unstructured":"Corbel M. Food and Agriculture Organization of the United Nations, World Organisation for Animal Health. World Health Organization Brucellosis in Humans and Animals WHO\/CDS\/EPR\/20067. Geneva: WHO Press; 2006. pp.4\u201310."},{"key":"4660_CR6","doi-asserted-by":"crossref","unstructured":"Yaeger MJ, Holler LD. Bacterial causes of bovine infertility and abortion. In: Youngquist RS and Walter R, editors. Current therapy in large animal theriogenology. Saunders; 2007. pp. 389\u2013399.","DOI":"10.1016\/B978-072169323-1.50052-0"},{"issue":"6","key":"4660_CR7","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1016\/j.meegid.2009.07.001","volume":"9","author":"AM Whatmore","year":"2009","unstructured":"Whatmore AM. Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infect Genet Evol. 2009;9(6):1168\u201384.","journal-title":"Infect Genet Evol"},{"issue":"1\u20132","key":"4660_CR8","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.smallrumres.2006.09.007","volume":"68","author":"A Pandya","year":"2007","unstructured":"Pandya A, Ghodke K. Goat and sheep milk products other than cheeses and yoghurt. Small Rumin Res. 2007;68(1\u20132):193\u2013206.","journal-title":"Small Rumin Res"},{"issue":"1","key":"4660_CR9","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1016\/j.ijfoodmicro.2010.11.033","volume":"145","author":"A Falenski","year":"2011","unstructured":"Falenski A, Mayer-Scholl A, Filter M, G\u00f6llner C, Appel B, N\u00f6ckler K. Survival of Brucella spp. in mineral water, milk and yogurt. Int J Food Microbiol. 2011;145(1):326\u201330.","journal-title":"Int J Food Microbiol."},{"issue":"2","key":"4660_CR10","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.ijid.2007.04.019","volume":"12","author":"M Sofian","year":"2008","unstructured":"Sofian M, Aghakhani A, Velayati AA, Banifazl M, Eslamifar A, Ramezani A. Risk factors for human brucellosis in Iran: a case\u2013control study. Int J Infect Dis. 2008;12(2):157\u201361.","journal-title":"Int J Infect Dis"},{"issue":"1\u20132","key":"4660_CR11","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.smallrumres.2005.05.024","volume":"65","author":"V Gupta","year":"2006","unstructured":"Gupta V, Verma DK, Rout P, Singh S, Vihan V. Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Rumin Res. 2006;65(1\u20132):79\u201384.","journal-title":"Small Rumin Res"},{"key":"4660_CR12","unstructured":"Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the brucellosis laboratory. Paris: Institut National de la Recherche Agronomique (INRA); 1988."},{"issue":"11\u201312","key":"4660_CR13","first-page":"577","volume":"49","author":"S Al Dahouk","year":"2003","unstructured":"Al Dahouk S, Tomaso H, N\u00f6ckler K, Neubauer H, Frangoulidis D. Laboratory-based diagnosis of brucellosis-a review of the literature. Part II: serological tests for brucellosis. Clin Lab. 2003;49(11\u201312):577\u201389.","journal-title":"Clin Lab"},{"key":"4660_CR14","unstructured":"Bonfini B, Chiarenza G, Paci V, Sacchini F, Salini R, Vesco G, et al. Cross-reactivity in serological tests for brucellosis: a comparison of immune response of Escherichia coli O157: H7 and Yersinia enterocolitica O: 9 vs Brucella spp. Vet Ital. 2018;54(2):107\u201314."},{"key":"4660_CR15","first-page":"927","volume":"55","author":"M Corbel","year":"1985","unstructured":"Corbel M. Recent advances in the study of Brucella antigens and their serological cross-reactions. Vet Bull. 1985;55:927\u201342.","journal-title":"Vet Bull"},{"key":"4660_CR16","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1023\/A:1025165913274","volume":"19","author":"M Refai","year":"2003","unstructured":"Refai M. Application of biotechnology in the diagnosis and control of brucellosis in the Near East Region. World J Microbiol Biotechnol. 2003;19:443\u20139.","journal-title":"World J Microbiol Biotechnol"},{"issue":"2","key":"4660_CR17","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1051\/rnd:2002013","volume":"42","author":"M Boutinaud","year":"2002","unstructured":"Boutinaud M, Jammes H. Potential uses of milk epithelial cells: a review. Reprod Nutr Dev. 2002;42(2):133\u201347.","journal-title":"Reprod Nutr Dev"},{"key":"4660_CR18","unstructured":"Feligini M, Frati S, Cubric Curik V, Brambilla A, Parma P, Curik I, et al. Caprine \u03b1s1-casein polymorphism: characterisation of A, B, E and F variants by means of various biochemical and molecular techniques.\u00a0Food Technol Biotechnol. 2005;43(2):123\u201332."},{"issue":"7","key":"4660_CR19","doi-asserted-by":"publisher","first-page":"3550","DOI":"10.3168\/jds.2007-0149","volume":"90","author":"F d\u2019Angelo","year":"2007","unstructured":"d\u2019Angelo F, Santillo A, Sevi A, Albenzio M. A simple salting-out method for DNA extraction from milk somatic cells: investigation into the goat CSN1S1 gene. J Dairy Sci. 2007;90(7):3550\u20132.","journal-title":"J Dairy Sci"},{"issue":"11","key":"4660_CR20","doi-asserted-by":"publisher","first-page":"6804","DOI":"10.3168\/jds.2014-8066","volume":"97","author":"Y Liu","year":"2014","unstructured":"Liu Y, Gao J, Yang Y, Ku T, Zan L. Novel extraction method of genomic DNA suitable for long-fragment amplification from small amounts of milk. J Dairy Sci. 2014;97(11):6804\u20139.","journal-title":"J Dairy Sci"},{"issue":"3","key":"4660_CR21","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1053\/tvjl.2001.0681","volume":"163","author":"ME Hamdy","year":"2002","unstructured":"Hamdy ME, Amin A. Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Vet J. 2002;163(3):299\u2013305.","journal-title":"Vet J"},{"issue":"2","key":"4660_CR22","doi-asserted-by":"publisher","first-page":"141","DOI":"10.4067\/S0301-732X2008000200005","volume":"40","author":"Z Ilhan","year":"2008","unstructured":"Ilhan Z, Boynukara B, Solmaz H, Ekin I, Aksakal A, Gulhan T. Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay. Archivos de medicina veterinaria. 2008;40(2):141\u20136.","journal-title":"Archivos de medicina veterinaria"},{"key":"4660_CR23","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.3855\/jidc.4847","volume":"10","author":"G Wareth","year":"2014","unstructured":"Wareth G, Melzer F, Elschner MC, Neubauer H, Roesler U. Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR. J Infect Dev Ctries. 2014;10:1339\u201343.","journal-title":"J Infect Dev Ctries"},{"issue":"1","key":"4660_CR24","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1080\/10495398.2020.1777149","volume":"33","author":"H Rahimi","year":"2022","unstructured":"Rahimi H, Tukmechi A, Rashidian E. Use of touch-down polymerase chain reaction to enhance the sensitivity of Brucella melitensis detection in raw milk. Anim Biotechnol. 2022;33(1):104\u20139.","journal-title":"Anim Biotechnol"},{"issue":"1","key":"4660_CR25","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1111\/j.1574-6941.2008.00629.x","volume":"67","author":"CJ Smith","year":"2009","unstructured":"Smith CJ, Osborn AM. Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol. 2009;67(1):6\u201320.","journal-title":"FEMS Microbiol Ecol"},{"issue":"11","key":"4660_CR26","doi-asserted-by":"publisher","first-page":"e0236849","DOI":"10.1371\/journal.pone.0236849","volume":"15","author":"TN Furstenau","year":"2020","unstructured":"Furstenau TN, Cocking JH, Hepp CM, Fofanov VY. Sample pooling methods for efficient pathogen screening: practical implications. PLoS One. 2020;15(11):e0236849.","journal-title":"PLoS One"},{"issue":"1\u20134","key":"4660_CR27","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/S0378-1135(02)00248-1","volume":"90","author":"M Refai","year":"2002","unstructured":"Refai M. Incidence and control of brucellosis in the Near East region. Vet Microbiol. 2002;90(1\u20134):81\u2013110.","journal-title":"Vet Microbiol"},{"issue":"3","key":"4660_CR28","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1051\/vetres:2005003","volume":"36","author":"J Godfroid","year":"2005","unstructured":"Godfroid J, Cloeckaert A, Liautard J-P, Kohler S, Fretin D, Walravens K, et al. From the discovery of the Malta fever\u2019s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet Res. 2005;36(3):313\u201326.","journal-title":"Vet Res"},{"issue":"4","key":"4660_CR29","doi-asserted-by":"publisher","first-page":"289","DOI":"10.3325\/cmj.2010.51.289","volume":"51","author":"M Gwida","year":"2010","unstructured":"Gwida M, Al Dahouk S, Melzer F, R\u00f6sler U, Neubauer H, Tomaso H. Brucellosis\u2013regionally emerging zoonotic disease? Croat Med J. 2010;51(4):289\u201395.","journal-title":"Croat Med J"},{"issue":"1","key":"4660_CR30","first-page":"95","volume":"27","author":"JM Blasco","year":"2011","unstructured":"Blasco JM, Molina-Flores B. Control and eradication of Brucella melitensis infection in sheep and goats. Veterinary Clinics: Food Animal Practice. 2011;27(1):95\u2013104.","journal-title":"Vet Clin : Food Anim Pract"},{"issue":"1\u20132","key":"4660_CR31","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.smallrumres.2005.07.032","volume":"62","author":"A Benkirane","year":"2006","unstructured":"Benkirane A. Ovine and caprine brucellosis: world distribution and control\/eradication strategies in West Asia\/North Africa region. Small Rumin Res. 2006;62(1\u20132):19\u201325.","journal-title":"Small Rumin Res"},{"issue":"1\u20134","key":"4660_CR32","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/S0378-1135(02)00231-6","volume":"90","author":"M Banai","year":"2002","unstructured":"Banai M. Control of small ruminant brucellosis by use of Brucella melitensis Rev. 1 vaccine: laboratory aspects and field observations. Vet Microbiol. 2002;90(1\u20134):497\u2013519.","journal-title":"Vet Microbiol."},{"key":"4660_CR33","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1007\/s11250-018-1706-4","volume":"51","author":"C Castelo","year":"2019","unstructured":"Castelo C, Sim\u00f5es J. Risk factors of brucellosis (re-) incidence in sheep and goat flocks in an endemic area of Portugal. Trop Anim Health Prod. 2019;51:487\u201390.","journal-title":"Trop Anim Health Prod"},{"issue":"2","key":"4660_CR34","doi-asserted-by":"publisher","first-page":"379","DOI":"10.20506\/rst.7.2.356","volume":"7","author":"E Zowghi","year":"1988","unstructured":"Zowghi E, Ebadi A. Abortion due to Brucella abortus in sheep in Iran. Revue Scientifique et Technique, Office International Des \u00c9pizooties. 1988;7(2):379\u201382.","journal-title":"Revue Scientifique et Technique, Office International Des \u00c9pizooties"},{"issue":"3","key":"4660_CR35","doi-asserted-by":"publisher","first-page":"973","DOI":"10.20506\/rst.24.3.1627","volume":"24","author":"R Ocholi","year":"2005","unstructured":"Ocholi R, Kwaga J, Ajogi I, Bale J. Abortion due to Brucella abortus in sheep in Nigeria. Revue Scientifique et Technique-Office International Des Epizooties. 2005;24(3):973\u201380.","journal-title":"Revue Scientifique et Technique-Office International Des Epizooties"},{"issue":"1","key":"4660_CR36","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13104-015-1173-1","volume":"8","author":"G Wareth","year":"2015","unstructured":"Wareth G, Melzer F, Tomaso H, Roesler U, Neubauer H. Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR. BMC Res Notes. 2015;8(1):1\u20135.","journal-title":"BMC Res Notes"},{"issue":"20","key":"4660_CR37","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1136\/vr.144.20.555","volume":"144","author":"M Grill\u00f3","year":"1999","unstructured":"Grill\u00f3 M, Mar\u00edn M, Barber\u00e1n M, Blasco J. Experimental Brucella ovis infection in pregnant ewes. Vet Record. 1999;144(20):555\u20138.","journal-title":"Vet Record"},{"issue":"2","key":"4660_CR38","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/j.mimet.2008.07.002","volume":"75","author":"V Hini\u0107","year":"2008","unstructured":"Hini\u0107 V, Brodard I, Thomann A, Cvetni\u0107 \u017d, Makaya P, Frey J, et al. Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J Microbiol Methods. 2008;75(2):375\u20138.","journal-title":"J Microbiol Methods."},{"issue":"3","key":"4660_CR39","doi-asserted-by":"publisher","first-page":"e000536","DOI":"10.1371\/journal.pntd.0005367","volume":"11","author":"E Lindahl-Rajala","year":"2017","unstructured":"Lindahl-Rajala E, Hoffman T, Fretin D, Godfroid J, Sattorov N, Boqvist S, et al. Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan. PLoS Negl Trop Dis. 2017;11(3):e000536.","journal-title":"PLoS Negl Trop Dis."},{"issue":"1","key":"4660_CR40","first-page":"15","volume":"58","author":"S Surucuoglu","year":"2009","unstructured":"Surucuoglu S, El S, Ural S, Gazi H, Kurutepe S, Taskiran P, et al. Evaluation of real-time PCR method for rapid diagnosis of brucellosis with different clinical manifestations. Pol J Microbiol. 2009;58(1):15.","journal-title":"Pol J Microbiol"},{"issue":"170","key":"4660_CR41","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.ijfoodmicro.2013.10.022","volume":"17","author":"K Mertens","year":"2014","unstructured":"Mertens K, Freund L, Schmoock G, H\u00e4nsel C, Melzer F, Elschner MC. Comparative evaluation of eleven commercial DNA extraction kits for real-time PCR detection of Bacillus anthracis spores in spiked dairy samples. Int J Food Microbiol. 2014;17(170):29\u201337.","journal-title":"Int J Food Microbiol"},{"issue":"3","key":"4660_CR42","doi-asserted-by":"publisher","first-page":"e0193671","DOI":"10.1371\/journal.pone.0193671","volume":"13","author":"SF Lima","year":"2018","unstructured":"Lima SF, Bicalho MLdS, Bicalho RC. Evaluation of milk sample fractions for characterization of milk microbiota from healthy and clinical mastitis cows. PLoS One. 2018;13(3):e0193671.","journal-title":"PLoS One."},{"issue":"4","key":"4660_CR43","doi-asserted-by":"publisher","first-page":"1028","DOI":"10.1111\/jam.14377","volume":"127","author":"L Sun","year":"2019","unstructured":"Sun L, Dicksved J, Priyashantha H, Lundh \u00c5, Johansson M. Distribution of bacteria between different milk fractions, investigated using culture-dependent methods and molecular-based and fluorescent microscopy approaches. J Appl Microbiol. 2019;127(4):1028\u201337.","journal-title":"J Appl Microbiol"},{"issue":"Suppl 1","key":"4660_CR44","first-page":"56","volume":"23","author":"C Ernst","year":"1992","unstructured":"Ernst C, Dentine M. Genotyping of dairy cattle using direct amplification from somatic cells in small volumes of milk. Anim Genet. 1992;23(Suppl 1):56.","journal-title":"Anim Genet"},{"issue":"7","key":"4660_CR45","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.3168\/jds.S0022-0302(93)77536-0","volume":"76","author":"E Lipkin","year":"1993","unstructured":"Lipkin E, Shalom A, Khatib H, Soller M, Friedmann A. Milk as a source of deoxyribonucleic acid and as a substrate for the polymerase chain reaction. J Dairy Sci. 1993;76(7):2025\u201332.","journal-title":"J Dairy Sci"},{"issue":"2","key":"4660_CR46","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.mcp.2009.11.001","volume":"24","author":"A Psifidi","year":"2010","unstructured":"Psifidi A, Dovas CI, Banos G. A comparison of six methods for genomic DNA extraction suitable for PCR-based genotyping applications using ovine milk samples. Mol Cell Probes. 2010;24(2):93\u20138.","journal-title":"Mol Cell Probes"},{"issue":"2","key":"4660_CR47","doi-asserted-by":"publisher","first-page":"3319","DOI":"10.4238\/2014.April.29.10","volume":"13","author":"T Usman","year":"2014","unstructured":"Usman T, Yu Y, Liu C, Fan Z, Wang Y. Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk. Genet Mol Res. 2014;13(2):3319\u201328.","journal-title":"Genet Mol Res"},{"issue":"2","key":"4660_CR48","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1080\/10495398.2015.1116446","volume":"27","author":"J Pokorska","year":"2016","unstructured":"Pokorska J, Ku\u0142aj D, Dusza M, \u017bychli\u0144ska-Buczek J, Makulska J. New rapid method of DNA isolation from milk somatic cells. Anim Biotechnol. 2016;27(2):113\u20137.","journal-title":"Anim Biotechnol"},{"issue":"10","key":"4660_CR49","doi-asserted-by":"publisher","first-page":"3779","DOI":"10.3168\/jds.2008-1233","volume":"91","author":"C Marianelli","year":"2008","unstructured":"Marianelli C, Martucciello A, Tarantino M, Vecchio R, Iovane G, Galiero G. Evaluation of molecular methods for the detection of Brucella species in water buffalo milk. J Dairy Sci. 2008;91(10):3779\u201386.","journal-title":"J Dairy Sci"},{"issue":"1","key":"4660_CR50","doi-asserted-by":"publisher","first-page":"7","DOI":"10.5455\/umj.20151004054235","volume":"2","author":"Y Majid","year":"2016","unstructured":"Majid Y, et al. Prevalence of Brucella melitensis and Brucella abortus in raw milk and dairy product by real time PCR technique. Ulutas Med J. 2016;2(1):7\u201311.","journal-title":"Ulutas Med J."},{"key":"4660_CR51","doi-asserted-by":"publisher","first-page":"2252","DOI":"10.1590\/S0103-84782009005000167","volume":"39","author":"MN Xavier","year":"2009","unstructured":"Xavier MN, Costa \u00c9A, Paix\u00e3o TA, Santos RL. The genus Brucella and clinical manifestations of brucellosis. Ci\u00eancia Rural. 2009;39:2252\u201360.","journal-title":"Ci\u00eancia Rural"},{"key":"4660_CR52","doi-asserted-by":"crossref","unstructured":"D\u00edaz A. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Revue Scientifique et Technique-Office International Des Epizooties. 2013;32(1):53\u201360.","DOI":"10.20506\/rst.32.1.2187"},{"issue":"4","key":"4660_CR53","first-page":"18","volume":"15","author":"MJ Aminzadeh","year":"2023","unstructured":"Aminzadeh MJ, Kalateh Rahmani H, Hashemi K, Khaleghnia N, Azizzadeh M, Mirshokraei P. A comparison of bacteriological culture, serological and qPCR methods detecting Brucellosis in ewes with a history of abortion. Ranian J Vet Sci Technol. 2023;15(4):18\u201328.","journal-title":"Ranian J Vet Sci Technol."}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-025-04660-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-025-04660-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-025-04660-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T18:02:44Z","timestamp":1744221764000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-025-04660-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,9]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["4660"],"URL":"https:\/\/doi.org\/10.1186\/s12917-025-04660-9","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,9]]},"assertion":[{"value":"30 November 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors confirm that the ethical policies of the journal, as noted on the journal\u2019s author guidelines page, have been adhered to, and the appropriate ethical review committee approval has been received (research ethics committee of Ferdowsi University of Mashhad: IR.UM.REC.1401.063). The authors confirm that they have followed EU standards for the protection of animals used for scientific purposes. Moreover, the informed consent forms were properly obtained from the owners for all the research processes that were carried out on animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"256"}}