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This study screened 962 farmed goats and 237 small ruminants at informal markets for PPRV antibodies using c-ELISA. A subset (<jats:italic>n<\/jats:italic>\u2009=\u200929) was re-analysed with virus neutralisation assays (VNA) for antibodies to PPRV and CDV due to cross-reactivity concerns. c-ELISA indicated 6.44% PPRV seroprevalence on farms and 3.80% at markets. While VNA detected no PPRV antibodies, 45% (13\/29) tested positive for CDV, possibly suggesting cross-reactivity. Given the study\u2019s limitations, further research is needed to determine whether PPRV is present in Zambia and assess potential cross-reactivity.<\/jats:p>","DOI":"10.1186\/s12917-025-04732-w","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T04:51:45Z","timestamp":1745988705000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia"],"prefix":"10.1186","volume":"21","author":[{"given":"Sara","family":"Lysholm","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Logan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna F","family":"Lindahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikael","family":"Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elin","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pernilla Karlsson","family":"Bergkvist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Dautu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricky","family":"Chazya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian J.","family":"Willett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musso","family":"Munyeme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas Johansson","family":"Wensman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,30]]},"reference":[{"issue":"1","key":"4732_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5713\/ajas.2013.r.01","volume":"26","author":"C Devendra","year":"2013","unstructured":"Devendra C. 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For those who were illiterate, consent was given via a thumbprint. The data collection was performed in collaboration with the district veterinary office, and veterinary assistants or livestock officers were present at all study sites. At the Chibolya and Kasumbalesa markets, market officials were briefed about the study scope and asked for oral consent to collect samples. Additionally, oral informed consent was obtained from each participating trader. Participant anonymity and confidentiality were strictly maintained by collecting only personal details relevant for the study and ensuring that information pertaining to individual informants remained confidential outside of the research team. Ethical approval for the study, including but not limited to study methodology, informed consent procedure and means to ensure participant anonymity and confidentiality, was obtained from the International Livestock Research Institute Institutional Research Ethics Committee (ILRI-IREC2018-04\/1).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"303"}}