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A total of 576 healthy 80-day-old Wenchang chickens were randomly assigned to six experimental groups in a completely randomized design. The control group (Control) received a basal diet, while the antibiotic-treated group was supplemented with 2\u00a0g\/kg chlortetracycline hydrochloride (CTC) as a positive control. Four experimental groups were supplemented with different concentrations of MSCP: 400\u00a0mg\/kg (MSCP<jats:sub>400<\/jats:sub>), 800\u00a0mg\/kg (MSCP<jats:sub>800<\/jats:sub>), 1600\u00a0mg\/kg (MSCP<jats:sub>1600<\/jats:sub>), and 3200\u00a0mg\/kg (MSCP<jats:sub>3200<\/jats:sub>). The study employed a replicated pen design with 8 replicates per treatment group, each containing 12 birds. The results showed that dietary MSCP significantly increased the final body weight (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and average daily gain of chickens (<jats:italic>P<\/jats:italic>\u2009=\u20090.017), particularly at a dosage of 800\u00a0mg\/kg. We also found that serum CAT level (<jats:italic>P<\/jats:italic>\u2009=\u20090.030) and GSH-Px level (<jats:italic>P<\/jats:italic>\u2009=\u20090.011) were significantly higher in the MSCP group compared to the CTC group. Adding MSCP to the feed raised serum IL-4 level (<jats:italic>P<\/jats:italic>\u2009=\u20090.016) and significantly increased IgA level (<jats:italic>P<\/jats:italic>\u2009=\u20090.047) at 1600\u00a0mg\/kg. MSCP at 400 and 800\u00a0mg\/kg significantly increased villus height in the duodenum, jejunum, and ileum (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001 for duodenum and jejunum; <jats:italic>P<\/jats:italic>\u2009=\u20090.040 for ileum). Moreover, MSCP also increased the relative abundance of beneficial bacteria <jats:italic>Synergistota<\/jats:italic>, <jats:italic>Parabacteroides<\/jats:italic>, <jats:italic>Megamonas<\/jats:italic> and <jats:italic>Faecalibacterium<\/jats:italic>, and increased the diversity of intestinal flora. To sum up, adding MSCP to feed had a positive impact on production performance, comparable to antibiotics, particularly at a dosage of 800\u00a0mg\/kg. This suggests that MSCP is a promising, safe, and effective alternative to antibiotics in feed additives.<\/jats:p>","DOI":"10.1186\/s12917-025-04934-2","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T03:27:59Z","timestamp":1753241279000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dietary supplementation of polysaccharides from Millettia speciosa Champ. ex Benth on growth performance, immune function, antioxidant abilities and intestinal health of Wenchang chickens"],"prefix":"10.1186","volume":"21","author":[{"given":"Yu-hang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan-Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-ping","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-li","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu-ping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-Ping","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Min","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,23]]},"reference":[{"issue":"11","key":"4934_CR1","doi-asserted-by":"publisher","first-page":"1381","DOI":"10.1093\/bbb\/zbad110","volume":"87","author":"A Uehara","year":"2023","unstructured":"Uehara A, Maekawa M, Nakagawa K. 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