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A severe outbreak occurred in young Pekin ducklings on a commercial farm in Benha, Egypt.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The affected birds exhibited neurological signs, including lethargy, ataxia, and opisthotonus, leading to a high mortality rate. The livers and kidneys of ducklings showed various degrees of gross and histopathological lesions. Virus isolation trials in embryonated duck eggs revealed the characteristic greenish discoloration of the allantoic fluid, along with hepatitis and embryonic mortality. Furthermore, RT-PCR confirmed the presence of suspected duck hepatitis A virus type 1(DHAV-1). This study presents the first complete genome sequence of DHAV-1 from Egypt using next generation sequencing (NGS). Sequence analysis revealed that DHAV-1 exhibits the characteristic genomic organization of <jats:italic>Avihepatovirus<\/jats:italic>. The whole nucleotide sequence of Du\/Egy\/Benha\/2020\/DHAV-1 showed a high similarity to viruses isolated from Hungary in 2004, with a 99.9% identity in both the complete genome and structural genes (VP0, VP3, VP1). Antigenic analysis revealed a unique escape mutation, S178Y, related to conserved antigenic determinants on VP1 of DHAV-1 isolate. The cross-neutralization assay was utilized to assess the antigenic diversity between the field strain and the locally used live attenuated vaccine strain.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The results revealed minimal antigenic variation, highlighting the potential for immune evasion. These findings suggest that the currently administered vaccines in Egypt remain effective in controlling DHAV-1 infections. However, continuous surveillance is essential to monitor any emerging genetic or antigenic changes that could compromise vaccine efficacy in the future.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12917-025-05010-5","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T05:13:40Z","timestamp":1759468420000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Complete genome sequencing, molecular and antigenic characterization of duck hepatitis A virus type 1 isolated in Benha, Egypt"],"prefix":"10.1186","volume":"21","author":[{"given":"Ibrahim","family":"Moharam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"Abaza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norhan W.","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ehab M.","family":"El-Nahas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayman S.","family":"El-Habbaa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fares Z.","family":"Najar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Medhat","family":"Radi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman M.S.","family":"El Nagar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lamia","family":"Omar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maha A.N.","family":"Gamal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fouad S.","family":"El-Mayet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"key":"5010_CR1","doi-asserted-by":"crossref","unstructured":"Stoute ST, Tsai H, Metwally SA, Cheng A, Gu\u00e9rin J, Palya VJ et al. 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The study protocol was approved under the guidelines of the Institutional Animals Care and Use Committee (IACUC) Research Ethics Board, Faculty of Veterinary Medicine, Benha University with Approved Permission Number (BUFVTM 07-02-24).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not Applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"571"}}