{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:14:17Z","timestamp":1792552457349,"version":"4.5.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"DOI":"10.1186\/s12917-025-05031-0","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T08:02:59Z","timestamp":1760083379000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Increased kanamycin-resistant Salmonella Schwarzengrund, serovar changing and antimicrobial susceptibility of Salmonella enterica from broiler chickens in Kagoshima, Japan between 2019 and 2023"],"prefix":"10.1186","volume":"21","author":[{"given":"George J.","family":"Sanga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saki","family":"Hiramoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rika","family":"Miyajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vu Minh","family":"Duc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Obi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Shigemura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehisa","family":"Chuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"key":"5031_CR1","unstructured":"Food and Agriculture Organization of the United Nations, World Health Organization. Interventions for the Control of Non-typhoidal Salmonella spp. in Beef and Pork Microbiological Risk Assessment Series 30 Meeting Report and Systematic Review [Internet]. 2016. p. 1\u2013271. Available from: www.fao.org\/food\/food-safety-quality."},{"issue":"2","key":"5031_CR2","doi-asserted-by":"publisher","first-page":"321","DOI":"10.4315\/JFP-20-250","volume":"84","author":"S Boubendir","year":"2021","unstructured":"Boubendir S, Arsenault J, Quessy S, Thibodeau A, Fravalo P, Th\u00e9riault WP, Fournaise S, Gaucher M, Lou. Salmonella contamination of broiler chicken carcasses at critical steps of the slaughter process and in the environment of two slaughter plants: prevalence, genetic profiles, and association with the final carcass status. J Food Prot. 2021;84(2):321\u201332.","journal-title":"J Food Prot"},{"issue":"3","key":"5031_CR3","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1292\/jvms.61.255","volume":"61","author":"S Limawongpranee","year":"1999","unstructured":"Limawongpranee S, Hayashidani H, Okatani AT, Ono K, Hirota C, Kaneko KI, et al. Prevalence and persistence of Salmonella in broiler chicken flocks. J Vet Med Sci. 1999;61(3):255\u20139.","journal-title":"J Vet Med Sci"},{"key":"5031_CR4","doi-asserted-by":"publisher","unstructured":"Sasaki Y, Ikeda T, Momose Y, Yonemitsu K, Uema M, Asai T. Geographical variation of antimicrobial resistance of Salmonella in Japanese chicken. Food Saf. 2024;12(3):59\u201366. https:\/\/doi.org\/10.14252\/foodsafetyfscj.D-24-00002.","DOI":"10.14252\/foodsafetyfscj.D-24-00002"},{"issue":"1","key":"5031_CR5","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1836-6","volume":"15","author":"VM Duc","year":"2019","unstructured":"Duc VM, Nakamoto Y, Fujiwara A, Toyofuku H, Obi T, Chuma T. Prevalence of Salmonella in broiler chickens in Kagoshima, Japan in 2009 to 2012 and the relationship between serovars changing and antimicrobial resistance. BMC Vet Res. 2019;15(1):108.","journal-title":"BMC Vet Res"},{"issue":"3","key":"5031_CR6","doi-asserted-by":"publisher","first-page":"126","DOI":"10.14252\/foodsafetyfscj.2017019","volume":"6","author":"T Mori","year":"2018","unstructured":"Mori T, Okamura N, Kishino K, Wada S, Zou B, Nanba T, et al. Prevalence and antimicrobial resistance of Salmonella serotypes isolated from poultry meat in Japan. Food Saf. 2018;6(3):126\u20139.","journal-title":"Food Saf"},{"key":"5031_CR7","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10121541","author":"Y Sasaki","year":"2021","unstructured":"Sasaki Y, Kakizawa H, Baba Y, Ito T, Haremaki Y, Yonemichi M, et al. Antimicrobial resistance in Salmonella isolated from food workers and chicken products in Japan. Antibiotics. 2021. https:\/\/doi.org\/10.3390\/antibiotics10121541.","journal-title":"Antibiotics"},{"issue":"4","key":"5031_CR8","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.diagmicrobio.2018.10.016","volume":"93","author":"LZ Moreno","year":"2019","unstructured":"Moreno LZ, Gomes VTM, Moreira J, de Oliveira CH, Peres BP, Silva APS, et al. First report of mcr-1-harboring Salmonella enterica serovar Schwarzengrund isolated from poultry meat in Brazil. Diagn Microbiol Infect Dis. 2019;93(4):376\u20139. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2018.10.016.","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"3","key":"5031_CR9","doi-asserted-by":"publisher","first-page":"198","DOI":"10.7883\/yoken.JJID.2009.198","volume":"62","author":"T Asai","year":"2009","unstructured":"Asai T, Murakami K, Ozawa M, Koike R, Ishikawa H. Relationships between multidrug-resistant Salmonella enterica serovar Schwarzengrund and both broiler chickens and retail chicken meats in Japan. Jpn J Infect Dis. 2009;62(3):198\u2013200.","journal-title":"Jpn J Infect Dis"},{"issue":"5","key":"5031_CR10","doi-asserted-by":"publisher","first-page":"726","DOI":"10.3201\/eid1305.061489","volume":"13","author":"FM Aarestrup","year":"2007","unstructured":"Aarestrup FM, Hendriksen RS, Lockett J, Gay K, Teates K, McDermott PF, et al. International spread of multidrug-resistant Salmonella Schwarzengrund in food products. Emerg Infect Dis. 2007;13(5):726\u201331.","journal-title":"Emerg Infect Dis"},{"key":"5031_CR11","unstructured":"Ministry of Health, Labour and Welfare NI of ID. Salmonella serovars from human sources, 2014\u20132018 [Internet]. Infectious Agents Surveillance Report. Japan. 2018. Available from: https:\/\/www.niid.go.jp\/niid\/en\/iasr\/510-surveillance\/iasr\/graphs\/11742-iasrgbe2022."},{"issue":"1","key":"5031_CR12","doi-asserted-by":"publisher","first-page":"14","DOI":"10.7883\/yoken.JJID.2022.174","volume":"76","author":"NKH Obata","year":"2023","unstructured":"Obata NKH, Yokoyama K, Sadamasu K. Comparison of the serovars and characteristics of Salmonella isolated from human feces and foods in the 1990s and 2010s in Tok. Jpn J Infect Dis. 2023;76(1):14\u20139.","journal-title":"Jpn J Infect Dis"},{"issue":"2","key":"5031_CR13","doi-asserted-by":"publisher","first-page":"25","DOI":"10.14252\/foodsafetyfscj.D-24-00001","volume":"12","author":"Y Momose","year":"2024","unstructured":"Momose Y, Sasaki Y, Yonemitsu K, Kuroda M, Ikeda T, Uema M, et al. Changes in the phenotypes of Salmonella spp. in Japanese broiler flocks. Food Saf. 2024;12(2):25\u201333.","journal-title":"Food Saf"},{"issue":"5","key":"5031_CR14","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1292\/jvms.20-0096","volume":"82","author":"VM Duc","year":"2020","unstructured":"Duc VM, Shin J, Nagamatsu Y, Fuhiwara A, Toyofuku H, Obi T, et al. Increased Salmonella Schwarzengrund prevalence and antimicrobial susceptibility of Salmonella enterica isolated from broiler chickens in Kagoshima Prefecture in Japan between 2013 and 2016. J Vet Med Sci. 2020;82(5):585\u20139.","journal-title":"J Vet Med Sci"},{"issue":"May","key":"5031_CR15","first-page":"1","volume":"15","author":"MA Felix","year":"2024","unstructured":"Felix MA, Sopovski D, Commichaux S, Yoskowitz N, Aljahdali NH, Grim CJ, et al. Genetic relatedness and virulence potential of Salmonella Schwarzengrund strains with or without an IncFIB-IncFIC(FII) fusion plasmid isolated from food and clinical sources. Front Microbiol. 2024;15(May):1\u201312.","journal-title":"Front Microbiol"},{"key":"5031_CR16","unstructured":"National Veterinary Assay Laboratory Ministry of Agriculture F and F. 2023. Report on the Japanese Veterinary Antimicrobial Resistance Monitoring System. 2018; Available from: https:\/\/www.maff.go.jp\/nval\/yakuzai\/pdf\/JVARM_Report_2018-2019.pdf."},{"key":"5031_CR17","doi-asserted-by":"publisher","unstructured":"Shigemura H, Matsui M, Sekizuka T, Onozuka D, Noda T, Yamashita A, Kuroda M, Suzuki S, Kimura H, Fujimoto S, Oishi K, Sera N, Inoshima Y, Murakami K. Decrease in the prevalence of extended-spectrum cephalosporin-resistant Salmonella following cessation of ceftiofur use by the Japanese poultry industry. Int J Food Microbiol [Internet]. 2018;274(March):45\u201351. Available from: https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2018.03.011.","DOI":"10.1016\/j.ijfoodmicro.2018.03.011"},{"key":"5031_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vas.2022.100248","author":"VM Duc","year":"2022","unstructured":"Duc VM, Kakiuchi R, Muneyasu H, Toyofuku H, Obi T, Chuma T. Decreasing trend of \u03b2-lactam resistance in Salmonella isolates from broiler chickens due to the cessation of ceftiofur in ovo administration. Vet Anim Sci. 2022. https:\/\/doi.org\/10.1016\/j.vas.2022.100248.","journal-title":"Vet Anim Sci"},{"issue":"6","key":"5031_CR19","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1111\/j.1574-6976.2012.00337.x","volume":"36","author":"H Heuer","year":"2012","unstructured":"Heuer H, Smalla K. Plasmids foster diversification and adaptation of bacterial populations in soil. FEMS Microbiol Rev. 2012;36(6):1083\u2013104.","journal-title":"FEMS Microbiol Rev"},{"key":"5031_CR20","doi-asserted-by":"publisher","first-page":"167","DOI":"10.2147\/IDR.S48820","volume":"7","author":"JR Huddleston","year":"2014","unstructured":"Huddleston JR. Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes. Infect Drug Resist. 2014;7:167\u201376.","journal-title":"Infect Drug Resist"},{"issue":"4","key":"5031_CR21","doi-asserted-by":"publisher","first-page":"693","DOI":"10.26444\/aaem\/99528","volume":"25","author":"D Witkowska","year":"2018","unstructured":"Witkowska D, Kuncewicz M, \u017bebrowska JP, Sobczak J, Sowi\u0144ska J. Prevalence of Salmonella spp. in broiler chicken flocks in Northern Poland in 2014\u20132016. Ann Agric Environ Med. 2018;25(4):693\u20137.","journal-title":"Ann Agric Environ Med"},{"key":"5031_CR22","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13010092","author":"MA Felix","year":"2025","unstructured":"Felix MA, Han J, Khajanchi BK, Sanad YM, Zhao S, Foley SL. Salmonella enterica serovar Schwarzengrund: distribution, virulence, and antimicrobial resistance. Microorganisms. 2025. https:\/\/doi.org\/10.3390\/microorganisms13010092.","journal-title":"Microorganisms"},{"key":"5031_CR23","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10111336","author":"K Matsui","year":"2021","unstructured":"Matsui K, Nakazawa C, Khin STMM, Iwabuchi E, Asai T, Ishihara K. Molecular characteristics and antimicrobial resistance of Salmonella enterica serovar Schwarzengrund from chicken meat in Japan. Antibiotics. 2021. https:\/\/doi.org\/10.3390\/antibiotics10111336.","journal-title":"Antibiotics"},{"key":"5031_CR24","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00306-17","author":"BW Brunelle","year":"2017","unstructured":"Brunelle BW, Bearson BL, Bearson SMD, Casey TA. Multidrug-resistant Salmonella enterica serovar typhimurium isolates are resistant to antibiotics that influence their swimming and swarming motility. mSphere. 2017. https:\/\/doi.org\/10.1128\/mSphere.00306-17.","journal-title":"mSphere"},{"issue":"3","key":"5031_CR25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/antibiotics14030288","volume":"14","author":"K Ishihara","year":"2025","unstructured":"Ishihara K, Someno S, Matsui K, Nakazawa C, Abe T, Harima H, et al. Determination of antimicrobial resistance megaplasmid-like pESI structures contributing to the spread of Salmonella Schwarzengrund in Japan. Antibiotics. 2025;14(3):1\u201317.","journal-title":"Antibiotics"},{"issue":"12","key":"5031_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0015524","volume":"5","author":"TJ Johnson","year":"2010","unstructured":"Johnson TJ, Thorsness JL, Anderson CP, Lynne AM, Foley SL, Han J, et al. Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky. PLoS One. 2010;5(12):1\u201310.","journal-title":"PLoS One"},{"key":"5031_CR27","doi-asserted-by":"publisher","unstructured":"Sopovski D, Commichaux S, Zhao S, Grim CJ, Foley SL, Khajanchi BK. Complete genome sequences of 17 Salmonella enterica serovar Schwarzengrund isolates carrying an IncFIB-IncFIC (FII) fusion plasmid. Food Microbiol. 2024;13(2):104\u20137. https:\/\/doi.org\/10.1128\/mra.01062-23.","DOI":"10.1128\/mra.01062-23"},{"issue":"5","key":"5031_CR28","doi-asserted-by":"publisher","first-page":"968","DOI":"10.3382\/ps.2007-00455","volume":"87","author":"F Shahada","year":"2008","unstructured":"Shahada F, Chuma T, Okamoto K, Sueyoshi M. Temporal distribution and genetic fingerprinting of Salmonella in broiler flocks from Southern Japan. Poult Sci. 2008;87(5):968\u201372.","journal-title":"Poult Sci"},{"key":"5031_CR29","unstructured":"Grimont PAD, Weill FX, Who Collaborating Centre for Reference and Research on Salmonella. Inst Pasteur [Internet]. 2007;9th editio(January):1\u2013166. Available from: https:\/\/www.pasteur.fr\/ip\/portal\/action\/WebdriveActionEvent\/oid\/01s-000036-089."},{"key":"5031_CR30","unstructured":"Standards CLSI, Testing P, Melvin AS, Weinstein P, Thomas M, Kirn J Jr., MD P, James J, Lewis S, PharmD II, Brandi Limbago F, Replaces P, Amy M, Mathers J. MD D, Shelley Campeau, PhD D, Tony Mazzulli, MD, FACP F, editors. 2020. p.\u00a0332."},{"issue":"1\u20132","key":"5031_CR31","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.vetmic.2009.07.007","volume":"140","author":"F Shahada","year":"2010","unstructured":"Shahada F, Sugiyama H, Chuma T, Sueyoshi M, Okamoto K. Genetic analysis of multi-drug resistance and the clonal dissemination of \u03b2-lactam resistance in Salmonella infantis isolated from broilers. Vet Microbiol. 2010;140(1\u20132):136\u201341.","journal-title":"Vet Microbiol"},{"issue":"17","key":"5031_CR32","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","volume":"34","author":"S Chen","year":"2018","unstructured":"Chen S, Zhou Y, Chen Y, Gu J. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018;34(17):i884\u201390.","journal-title":"Bioinformatics"},{"issue":"15","key":"5031_CR33","doi-asserted-by":"publisher","first-page":"2666","DOI":"10.1093\/bioinformatics\/bty149","volume":"34","author":"W De Coster","year":"2018","unstructured":"De Coster W, D\u2019Hert S, Schultz DT, Cruts M, Van Broeckhoven C. Nanopack: visualizing and processing long-read sequencing data. Bioinformatics. 2018;34(15):2666\u20139.","journal-title":"Bioinformatics"},{"issue":"6","key":"5031_CR34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pcbi.1005595","volume":"13","author":"RR Wick","year":"2017","unstructured":"Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017;13(6):1\u201322.","journal-title":"PLoS Comput Biol"},{"issue":"5","key":"5031_CR35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1099\/mgen.0.001244","volume":"10","author":"G Bouras","year":"2024","unstructured":"Bouras G, Houtak G, Wick RR, Mallawaarachchi V, Roach MJ, Papudeshi B, Judd LM, Sheppard AE, Edwards RA, Vreugde S. Hybracter: enabling scalable, automated, complete and accurate bacterial genome assemblies. Microb Genomics. 2024;10(5):1\u201315.","journal-title":"Microb Genomics"},{"key":"5031_CR36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112963","author":"BJ Walker","year":"2014","unstructured":"Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, et al. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One. 2014. https:\/\/doi.org\/10.1371\/journal.pone.0112963.","journal-title":"PLoS One"},{"issue":"7","key":"5031_CR37","doi-asserted-by":"publisher","first-page":"3895","DOI":"10.1128\/AAC.02412-14","volume":"58","author":"A Carattoli","year":"2014","unstructured":"Carattoli A, Zankari E, Garci\u00e1-Fern\u00e1ndez A, Larsen MV, Lund O, Villa L, et al. In silico detection and typing of plasmids using plasmidfinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother. 2014;58(7):3895\u2013903.","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"5031_CR38","doi-asserted-by":"publisher","first-page":"2640","DOI":"10.1093\/jac\/dks261","volume":"67","author":"E Zankari","year":"2012","unstructured":"Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 2012;67(11):2640\u20134.","journal-title":"J Antimicrob Chemother"},{"issue":"2","key":"5031_CR39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/microorganisms10020292","volume":"10","author":"A Bharat","year":"2022","unstructured":"Bharat A, Petkau A, Avery BP, Chen J, Folster J, Carson CA, Kearney A, Nadon C, Mabon P, Thiessen J, Alexander DC, Allen V, El Bailey S, Bekal S, German GJ, Haldane D, Hoang L, Chui L, Minion J, Zahariadis G, Van Domselaar G, Reid-Smith RJ, Mulvey MR. Correlation between phenotypic and in Silico detection of antimicrobial resistance in in Canada using Staramr. Microorganisms. 2022;10(2):1\u201310.","journal-title":"Microorganisms"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-025-05031-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-025-05031-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-025-05031-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T11:54:08Z","timestamp":1760356448000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-025-05031-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,10]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["5031"],"URL":"https:\/\/doi.org\/10.1186\/s12917-025-05031-0","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,10]]},"assertion":[{"value":"26 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 August 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 October 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Specimen collection and carcass handling were conducted in accordance with Japanese regulations on poultry carcass inspection. The samples were released from the accredited poultry processing plant to the analysis laboratory under Japanese regulations on poultry processing. This study was approved by the Institutional Animal Care and Ethics Committee of the Joint Faculty of Veterinary Medicine, Kagoshima University.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"599"}}