{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:49:31Z","timestamp":1785894571691,"version":"3.56.0"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":65,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the Fangchenggang Science and Technology Program","award":["AB22013007"],"award-info":[{"award-number":["AB22013007"]}]},{"name":"Guangxi Science and Technology Program","award":["AA17204081-1"],"award-info":[{"award-number":["AA17204081-1"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"DOI":"10.1186\/s12917-026-05602-9","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T07:42:13Z","timestamp":1780299733000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["CRISPR-Cas9-mediated construction of a Streptococcus agalactiae vaccine for tilapia and evaluation of its protective efficacy"],"prefix":"10.1186","volume":"22","author":[{"given":"Mianlong","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiufang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taian","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghai","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonghe","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wende","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"issue":"4","key":"5602_CR1","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1111\/j.1365-2761.2007.00806.x","volume":"30","author":"DH Xu","year":"2007","unstructured":"Xu DH, Shoemaker CA, Klesius PH. Evaluation of the link between gyrodactylosis and streptococcosis of Nile tilapia, Oreochromis niloticus (L). J FISH DIS. 2007;30(4):233\u20138.","journal-title":"J FISH DIS"},{"issue":"1","key":"5602_CR2","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/S0044-8486(01)00667-6","volume":"205","author":"HT Nguyen","year":"2002","unstructured":"Nguyen HT, Kanai K, Yoshikoshi K. Ecological investigation of Streptococcus iniae in cultured Japanese flounder (Paralichthys olivaceus) using selective isolation procedures. Aquaculture. 2002;205(1):7\u201317.","journal-title":"Aquaculture"},{"issue":"4","key":"5602_CR3","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1007\/s12562-011-0365-4","volume":"77","author":"X Ye","year":"2011","unstructured":"Ye X, Li J, Lu M, Deng G, Jiang X, Tian Y, Quan Y, Jian Q. Identification and molecular typing of Streptococcus agalactiae isolated from pond-cultured tilapia in China. FISHERIES SCI. 2011;77(4):623\u201332.","journal-title":"FISHERIES SCI"},{"issue":"3\u20134","key":"5602_CR4","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1016\/j.vetmic.2012.04.035","volume":"159","author":"M Chen","year":"2012","unstructured":"Chen M, Li LP, Wang R, Liang WW, Huang Y, Li J, Lei AY, Huang WY, Gan X. PCR detection and PFGE genotype analyses of streptococcal clinical isolates from tilapia in China. VET MICROBIOL. 2012;159(3\u20134):526\u201330.","journal-title":"VET MICROBIOL"},{"issue":"1","key":"5602_CR5","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.fsi.2014.01.011","volume":"37","author":"M Nur-Nazifah","year":"2014","unstructured":"Nur-Nazifah M, Sabri MY, Siti-Zahrah A. Development and efficacy of feed-based recombinant vaccine encoding the cell wall surface anchor family protein of Streptococcus agalactiae against streptococcosis in Oreochromis sp. FISH SHELLFISH IMMUN. 2014;37(1):193\u2013200.","journal-title":"FISH SHELLFISH IMMUN"},{"issue":"1","key":"5602_CR6","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1038\/s41598-019-57283-0","volume":"10","author":"A Pumchan","year":"2020","unstructured":"Pumchan A, Krobthong S, Roytrakul S, Sawatdichaikul O, Kondo H, Hirono I, Areechon N, Unajak S. Novel Chimeric Multiepitope Vaccine for Streptococcosis Disease in Nile Tilapia (Oreochromis niloticus Linn). SCI REP-UK. 2020;10(1):603.","journal-title":"SCI REP-UK"},{"issue":"1","key":"5602_CR7","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1186\/s12864-015-2026-y","volume":"16","author":"R Wang","year":"2015","unstructured":"Wang R, Li L, Huang Y, Luo F, Liang W, Gan X, Huang T, Lei A, Chen M, Chen L. Comparative genome analysis identifies two large deletions in the genome of highly-passaged attenuated Streptococcus agalactiae strain YM001 compared to the parental pathogenic strain HN016. BMC Genomics. 2015;16(1):897.","journal-title":"BMC Genomics"},{"key":"5602_CR8","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1016\/j.fsi.2019.11.044","volume":"98","author":"D Zhang","year":"2020","unstructured":"Zhang D, Gao Y, Li Q, Ke X, Liu Z, Lu M, Shi C. An effective live attenuated vaccine against Streptococcus agalactiae infection in farmed Nile tilapia (Oreochromis niloticus). FISH SHELLFISH IMMUN. 2020;98:853\u20139.","journal-title":"FISH SHELLFISH IMMUN"},{"key":"5602_CR9","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1016\/j.fsi.2019.07.081","volume":"93","author":"L Li","year":"2019","unstructured":"Li L, Liu Y, Huang T, Liang W, Chen M. Development of an attenuated oral vaccine strain of tilapia Group B Streptococci serotype Ia by gene knockout technology. FISH SHELLFISH IMMUN. 2019;93:924\u201333.","journal-title":"FISH SHELLFISH IMMUN"},{"key":"5602_CR10","first-page":"100538","volume":"18","author":"Y Cai","year":"2020","unstructured":"Cai Y, Liu Z, Lu M, Ke X, Zhang D, Gao F, Cao J, Wang M, Yi M. Oral immunization with surface immunogenic protein from Streptococcus agalactiae expressed in Lactococcus lactis induces protective immune responses of tilapia (Oreochromis niloticus). AQUACULT REP. 2020;18:100538.","journal-title":"AQUACULT REP"},{"key":"5602_CR11","doi-asserted-by":"publisher","first-page":"123770","DOI":"10.1016\/j.ijbiomac.2023.123770","volume":"235","author":"C Zhu","year":"2023","unstructured":"Zhu C, Zhang N, Jing D, Liu X, Zeng Z, Wang J, Xiao F, Zhang H, Chi H, Wan C, et al. Characterization and evaluation of an oral vaccine via nano-carrier for surface immunogenic protein (Sip) delivery against Streptococcus agalactiae infection. INT J BIOL MACROMOL. 2023;235:123770.","journal-title":"INT J BIOL MACROMOL"},{"issue":"7","key":"5602_CR12","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.3390\/vaccines11071262","volume":"11","author":"Y Cao","year":"2023","unstructured":"Cao Y, Liu J, Liu G, Du H, Liu T, Wang G, Wang Q, Zhou Y, Wang E. Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against Streptococcus Infection in Tilapia (Oreochromis niloticus). VACCINES-BASEL. 2023;11(7):1262.","journal-title":"VACCINES-BASEL"},{"key":"5602_CR13","doi-asserted-by":"publisher","first-page":"109353","DOI":"10.1016\/j.fsi.2023.109353","volume":"145","author":"C Lu","year":"2024","unstructured":"Lu C, Wangkahart E, Huang J, Huang Y, Huang Y, Cai J, Jian J, Wang B. Immune response and protective efficacy of Streptococcus agalactiae vaccine coated with chitosan oligosaccharide for different immunization strategy in nile tilapia (Oreochromis niloticus). FISH SHELLFISH IMMUN. 2024;145:109353.","journal-title":"FISH SHELLFISH IMMUN"},{"issue":"12","key":"5602_CR14","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1016\/j.dci.2011.03.007","volume":"35","author":"KP Plant","year":"2011","unstructured":"Plant KP, LaPatra SE. Advances in fish vaccine delivery. Dev Comp Immunol. 2011;35(12):1256\u201362.","journal-title":"Dev Comp Immunol"},{"issue":"6096","key":"5602_CR15","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1126\/science.1225829","volume":"337","author":"M Jinek","year":"2012","unstructured":"Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337(6096):816\u201321.","journal-title":"Science"},{"issue":"1","key":"5602_CR16","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1038\/s41467-025-56278-y","volume":"16","author":"DG Kim","year":"2025","unstructured":"Kim DG, Gu B, Cha Y, Ha J, Lee Y, Kim G, Cho B, Oh M. Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production. NAT COMMUN. 2025;16(1):874.","journal-title":"NAT COMMUN"},{"issue":"4","key":"5602_CR17","doi-asserted-by":"publisher","first-page":"1190","DOI":"10.1016\/j.synbio.2025.06.010","volume":"10","author":"Z Ouyang","year":"2025","unstructured":"Ouyang Z, Zhang X, Hou X, Huang J, Lin Y, Zheng S. Development of a CRISPR\/Cas9 genome editing toolbox for Corynebacterium stationis and its application in hypoxanthine biosynthesis. Synth Syst Biotechnol. 2025;10(4):1190\u20139.","journal-title":"Synth Syst Biotechnol"},{"issue":"11","key":"5602_CR18","doi-asserted-by":"publisher","first-page":"6409","DOI":"10.1128\/IAI.70.11.6409-6415.2002","volume":"70","author":"Q Cheng","year":"2002","unstructured":"Cheng Q, Debol S, Lam H, Eby R, Edwards L, Matsuka Y, Olmsted SB, Cleary PP. Immunization with C5a Peptidase or Peptidase-Type III Polysaccharide Conjugate Vaccines Enhances Clearance of Group B Streptococci from Lungs of Infected Mice. INFECT IMMUN. 2002;70(11):6409\u201315.","journal-title":"INFECT IMMUN"},{"issue":"19","key":"5602_CR19","doi-asserted-by":"publisher","first-page":"4839","DOI":"10.1046\/j.1432-1033.2002.03183.x","volume":"269","author":"ET Anderson","year":"2002","unstructured":"Anderson ET, Wetherell MG, Winter LA, Olmsted SB, Cleary PP, Matsuka YV. Processing, stability, and kinetic parameters of C5a peptidase from Streptococcus pyogenes. Eur J Biochem. 2002;269(19):4839\u201351.","journal-title":"Eur J Biochem"},{"issue":"08","key":"5602_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4236\/oalib.1102886","volume":"03","author":"X Ke","year":"2016","unstructured":"Ke X, Li Q, Li X, Liu Z, Lu M, Yang H. Construction and Analysis of the Immune Effects of a Streptococcus agalactiae Surface Protein ScpB Vaccine Encapsulated with Polylactic-Co-Glycolic Acid (PLGA). OALib. 2016;03(08):1\u201311.","journal-title":"OALib"},{"issue":"1","key":"5602_CR21","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1186\/s40168-025-02078-x","volume":"13","author":"J Xing","year":"2025","unstructured":"Xing J, Niu T, Yu T, Zou B, Shi C, Wang Y, Fan S, Li M, Bao M, Sun Y, et al. Faecalibacterium prausnitzii-derived outer membrane vesicles reprogram gut microbiota metabolism to alleviate Porcine Epidemic Diarrhea Virus infection. MICROBIOME. 2025;13(1):90.","journal-title":"MICROBIOME"},{"issue":"1","key":"5602_CR22","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1186\/s40168-022-01409-6","volume":"10","author":"Z Wu","year":"2022","unstructured":"Wu Z, Zhang Q, Yang J, Zhang J, Fu J, Dang C, Liu M, Wang S, Lin Y, Hao J, et al. Significant alterations of intestinal symbiotic microbiota induced by intraperitoneal vaccination mediate changes in intestinal metabolism of NEW Genetically Improved Farmed Tilapia (NEW GIFT, Oreochromis niloticus). MICROBIOME. 2022;10(1):221.","journal-title":"MICROBIOME"},{"issue":"12","key":"5602_CR23","doi-asserted-by":"publisher","first-page":"1811","DOI":"10.3390\/vaccines11121811","volume":"11","author":"S McCabe","year":"2023","unstructured":"McCabe S, Bj\u00e5nes E, Hendriks A, Wang Z, van Sorge NM, Pill-Pepe L, Bautista L, Chu E, Cod\u00e9e JDC, Fairman J, et al. The Group A Streptococcal Vaccine Candidate VAX-A1 Protects against Group B Streptococcus Infection via Cross-Reactive IgG Targeting Virulence Factor C5a Peptidase. VACCINES-BASEL. 2023;11(12):1811.","journal-title":"VACCINES-BASEL"},{"issue":"11","key":"5602_CR24","doi-asserted-by":"publisher","first-page":"3253","DOI":"10.1038\/s41596-023-00882-z","volume":"18","author":"D Pankratz","year":"2023","unstructured":"Pankratz D, Gomez NO, Nielsen A, Mustafayeva A, G\u00fcr M, Arce-Rodriguez F, Nikel PI, H\u00e4ussler S, Arce-Rodriguez A. An expanded CRISPR-Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates. NAT PROTOC. 2023;18(11):3253\u201388.","journal-title":"NAT PROTOC"},{"key":"5602_CR25","doi-asserted-by":"publisher","first-page":"735484","DOI":"10.1016\/j.aquaculture.2020.735484","volume":"528","author":"FC Ramos-Espinoza","year":"2020","unstructured":"Ramos-Espinoza FC, Cueva-Quiroz VA, Yunis-Aguinaga J, de Moraes JRE. A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus. Aquaculture. 2020;528:735484.","journal-title":"Aquaculture"},{"key":"5602_CR26","doi-asserted-by":"publisher","first-page":"108563","DOI":"10.1016\/j.fsi.2023.108563","volume":"134","author":"E Wangkahart","year":"2023","unstructured":"Wangkahart E, Thongsrisuk A, Vialle R, Pholchamat S, Sunthamala P, Phudkliang J, Srisapoome P, Wang T, Secombes CJ. Comparative study of the effects of Montanide\u2122 ISA 763A VG and ISA 763B VG adjuvants on the immune response against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus). FISH SHELLFISH IMMUN. 2023;134:108563.","journal-title":"FISH SHELLFISH IMMUN"},{"key":"5602_CR27","doi-asserted-by":"crossref","unstructured":"Ma T, Li F, Wu C, Lin J, Chen M, Zhang Y, Zeng H. Evaluation of MarR-deleted Streptococcus agalactiae as a live-attenuated vaccine candidate for Nile Tilapia (Oreochromis niloticus). Aquaculture. 2026;612:743195.","DOI":"10.1016\/j.aquaculture.2025.743195"},{"key":"5602_CR28","doi-asserted-by":"publisher","first-page":"111074","DOI":"10.1016\/j.fsi.2025.111074","volume":"169","author":"R Guha","year":"2026","unstructured":"Guha R, Lakshmi S, Krebs T, Schroers VJ, Adamek M, Elumalai P. Efficacy of a novel oral bivalent vaccine with fucoidan as adjuvant against Aeromonas hydrophila and Edwardsiella tarda infections in Nile tilapia aquaculture. FISH SHELLFISH IMMUN. 2026;169:111074.","journal-title":"FISH SHELLFISH IMMUN"},{"key":"5602_CR29","doi-asserted-by":"publisher","first-page":"110917","DOI":"10.1016\/j.fsi.2025.110917","volume":"168","author":"P Sunthamala","year":"2026","unstructured":"Sunthamala P, Wang T, Phadee P, Luang-In V, Srisapoome P, Zou J, Secombes CJ, Wangkahart E. IL-11 acts as a vaccine adjuvant in Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae, enhancing both cellular and humoral immune responses. FISH SHELLFISH IMMUN. 2026;168:110917.","journal-title":"FISH SHELLFISH IMMUN"},{"key":"5602_CR30","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/978-3-319-15774-0_8","volume":"850","author":"KS Kim","year":"2015","unstructured":"Kim KS, Surh CD. Induction of Immune Tolerance to Dietary Antigens. ADV EXP MED BIOL. 2015;850:93\u2013118.","journal-title":"ADV EXP MED BIOL"},{"issue":"8","key":"5602_CR31","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1084\/jem.20070590","volume":"204","author":"JL Coombes","year":"2007","unstructured":"Coombes JL, Siddiqui KRR, Arancibia-C\u00e1rcamo CV, Hall J, Sun C, Belkaid Y, Powrie F. A functionally specialized population of mucosal CD103\u2009+\u2009DCs induces Foxp3\u2009+\u2009regulatory T cells via a TGF-\u03b2\u2013 and retinoic acid\u2013dependent mechanism. J Exp Med. 2007;204(8):1757\u201364.","journal-title":"J Exp Med"},{"key":"5602_CR32","doi-asserted-by":"publisher","first-page":"109509","DOI":"10.1016\/j.fsi.2024.109509","volume":"148","author":"M Ortiz","year":"2024","unstructured":"Ortiz M, Esteban M\u00c1. Biology and functions of fish thrombocytes: A review. FISH SHELLFISH IMMUN. 2024;148:109509.","journal-title":"FISH SHELLFISH IMMUN"},{"key":"5602_CR33","doi-asserted-by":"publisher","first-page":"110030","DOI":"10.1016\/j.fsi.2024.110030","volume":"155","author":"J Zhu","year":"2024","unstructured":"Zhu J, Li D, Xiao W, Yu J, Chen B, Zou Z, Yang H. Survival, serum biochemical parameters, hepatic antioxidant status, and gene expression of three Nile tilapia strains under pathogenic Streptococcus agalactiae challenge. FISH SHELLFISH IMMUN. 2024;155:110030.","journal-title":"FISH SHELLFISH IMMUN"},{"issue":"5","key":"5602_CR34","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1007\/s11882-015-0524-2","volume":"15","author":"S Steinmeyer","year":"2015","unstructured":"Steinmeyer S, Lee K, Jayaraman A, Alaniz RC. Microbiota Metabolite Regulation of Host Immune Homeostasis: A Mechanistic Missing Link. CURR ALLERGY ASTHM R. 2015;15(5):24.","journal-title":"CURR ALLERGY ASTHM R"},{"issue":"7","key":"5602_CR35","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.tim.2011.05.006","volume":"19","author":"E Holmes","year":"2011","unstructured":"Holmes E, Li JV, Athanasiou T, Ashrafian H, Nicholson JK. Understanding the role of gut microbiome\u2013host metabolic signal disruption in health and disease. TRENDS MICROBIOL. 2011;19(7):349\u201359.","journal-title":"TRENDS MICROBIOL"},{"issue":"1","key":"5602_CR36","doi-asserted-by":"publisher","first-page":"2563709","DOI":"10.1080\/19490976.2025.2563709","volume":"17","author":"Q Lu","year":"2025","unstructured":"Lu Q, Feng Y, Wang H, Zhu K, Teng L, Yue M, Li Y. Gut microbiota as a regulator of vaccine efficacy: implications for personalized vaccination. Gut Microbes. 2025;17(1):2563709.","journal-title":"Gut Microbes"},{"key":"5602_CR37","doi-asserted-by":"crossref","unstructured":"Sutra J, Hashim AM, Yusof MT, Al-Saari N, Nasruddin NS, Saad MZ, Zahaludin A, Yasin ISM, Azmai MNA. Dynamics of the gut microbiome of Asian seabass (Lates calcarifer) following oral vaccination and challenge with virulent Vibrio harveyi. Aquaculture. 2025;603:742420.","DOI":"10.1016\/j.aquaculture.2025.742420"},{"issue":"18","key":"5602_CR38","doi-asserted-by":"publisher","first-page":"7943","DOI":"10.1007\/s00253-020-10819-1","volume":"104","author":"C Huang","year":"2020","unstructured":"Huang C, Guo L, Wang J, Wang N, Huo Y. Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering. APPL MICROBIOL BIOT. 2020;104(18):7943\u201356.","journal-title":"APPL MICROBIOL BIOT"},{"key":"5602_CR39","doi-asserted-by":"publisher","unstructured":"Green R, Rogers EJ. Transformation of Chemically Competent E.coli. Methods in Enzymology. Volume 529. Elsevier; 2013. pp. 329\u201336. https:\/\/doi.org\/10.1016\/B978-0-12-418687-3.00028-8","DOI":"10.1016\/B978-0-12-418687-3.00028-8"},{"key":"5602_CR40","doi-asserted-by":"crossref","unstructured":"Ramos-Espinoza FC, Cueva-Quiroz VA, Yunis-Aguinaga J, Alvarez-Rubio NC, Paganoti De Mello N, Engr\u00e1cia, De Moraes JR. Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings. Fish Shellfish Immun. 2020;105:350\u2013358.","DOI":"10.1016\/j.fsi.2020.07.051"},{"issue":"9","key":"5602_CR41","doi-asserted-by":"publisher","first-page":"e1008765","DOI":"10.1371\/journal.ppat.1008765","volume":"16","author":"W Liu","year":"2020","unstructured":"Liu W, Zhang Y, Ma J, Jiang N, Fan Y, Zhou Y, Cain K, Yi M, Jia K, Wen H, et al. Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia. PLOS PATHOG. 2020;16(9):e1008765.","journal-title":"PLOS PATHOG"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12917-026-05602-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-026-05602-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12917-026-05602-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:15:48Z","timestamp":1785892548000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12917-026-05602-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,1]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["5602"],"URL":"https:\/\/doi.org\/10.1186\/s12917-026-05602-9","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-9209433\/v1","asserted-by":"object"}]},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,1]]},"assertion":[{"value":"24 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All animal experiments were conducted in strict compliance with standardized procedures and obtained approval from the Animal Ethics Review Committee of Guangxi University (Approval No.: GXU-2025-282). All the methods and procedures of this study comply with the requirements of the ARRIVE Guidelines.","order":1,"name":"Ethics","label":"Ethics approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The manuscript has been given publication permission by all authors.Not applicable.","order":2,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"456"}}