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However, the underlying mechanisms remain unclear.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The gene expression profiles of GSE83453, GSE51472, and GSE12644 were analyzed by \u2018limma\u2019 and \u2018weighted gene co-expression network analysis (WGCNA)\u2019 package in R to identify differentially expressed genes (DEGs) and key modules associated with CAVD, respectively. Then, enrichment analysis was performed based on Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, DisGeNET, and TRRUST database. Protein\u2013protein interaction network was constructed using the overlapped genes of DEGs and key modules, and we identified the top 5 hub genes by mixed character calculation.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We identified the blue and yellow modules as the key modules. Enrichment analysis showed that leukocyte migration, extracellular matrix, and extracellular matrix structural constituent were significantly enriched. <jats:italic>SPP1<\/jats:italic>, <jats:italic>TNC<\/jats:italic>, <jats:italic>SCG2<\/jats:italic>, <jats:italic>FAM20A<\/jats:italic>, and <jats:italic>CD52<\/jats:italic> were identified as hub genes, and their expression levels in calcified or normal aortic valve samples were illustrated, respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>This study suggested that <jats:italic>SPP1<\/jats:italic>, <jats:italic>TNC<\/jats:italic>, <jats:italic>SCG2<\/jats:italic>, <jats:italic>FAM20A<\/jats:italic>, and <jats:italic>CD52<\/jats:italic> might be hub genes associated with CAVD. Further studies are required to elucidate the underlying mechanisms and provide potential therapeutic targets.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12920-021-00989-w","type":"journal-article","created":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T08:03:04Z","timestamp":1621584184000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Identification of key genes in calcific aortic valve disease via weighted gene co-expression network analysis"],"prefix":"10.1186","volume":"14","author":[{"given":"Jin-Yu","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Yan","family":"Kan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Qing","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue-Yun","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,21]]},"reference":[{"issue":"9","key":"989_CR1","doi-asserted-by":"publisher","first-page":"962","DOI":"10.1016\/j.cjca.2014.03.022","volume":"30","author":"B Iung","year":"2014","unstructured":"Iung B, Vahanian A. 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