{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:12:18Z","timestamp":1788808338824,"version":"build-2803163510"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Biomed Sci"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1186\/s12929-016-0245-4","type":"journal-article","created":{"date-parts":[[2016,2,17]],"date-time":"2016-02-17T07:25:44Z","timestamp":1455693944000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Novel synthetic benzimidazole-derived oligosaccharide, M3BIM, prevents ex vivo platelet aggregation and in vivo thromboembolism"],"prefix":"10.1186","volume":"23","author":[{"given":"Ting-Lin","family":"Yen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Ping","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Li","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Bin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanasekaran","family":"Jayakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pitchairaj","family":"Geraldine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Ming","family":"Chou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Yau","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Jung","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joen-Rong","family":"Sheu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,2,17]]},"reference":[{"key":"245_CR1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.blre.2011.03.002","volume":"25","author":"K Broos","year":"2011","unstructured":"Broos K, Feys HB, De Meyer SF, Vanhoorelbeke K, Deckmyn H. Platelets at work in primary hemostasis. Blood Rev. 2011;25:155\u201367.","journal-title":"Blood Rev"},{"key":"245_CR2","doi-asserted-by":"crossref","first-page":"700","DOI":"10.3324\/haematol.2008.003178","volume":"94","author":"J Rivera","year":"2009","unstructured":"Rivera J, Lozano ML, Navarro-Nunez L, Vicente V. Platelet receptors and signaling in the dynamics of thrombus formation. Haematologica. 2009;94:700\u201311.","journal-title":"Haematologica"},{"key":"245_CR3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s00109-010-0691-5","volume":"89","author":"D Stegner","year":"2011","unstructured":"Stegner D, Nieswandt B. Platelet receptor signaling in thrombus formation. J Mol Med (Berl). 2011;89:109\u201321.","journal-title":"J Mol Med (Berl)"},{"key":"245_CR4","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1038\/nrd1198","volume":"2","author":"SP Jackson","year":"2003","unstructured":"Jackson SP, Schoenwaelder SM. Antiplatelet therapy: in search of the \u2018magic bullet\u2019. Nat Rev Drug Discov. 2003;2:775\u201389.","journal-title":"Nat Rev Drug Discov"},{"key":"245_CR5","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1517\/14656566.6.12.2027","volume":"6","author":"US Tantry","year":"2005","unstructured":"Tantry US, Bliden KP, Gurbel PA. Resistance to antiplatelet drugs: current status and future research. Expert Opin Pharmacother. 2005;6:2027\u201345.","journal-title":"Expert Opin Pharmacother"},{"key":"245_CR6","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.atherosclerosis.2005.02.034","volume":"183","author":"NF Worth","year":"2005","unstructured":"Worth NF, Berry CL, Thomas AC, Campbell JH. S18886, a selective TP receptor antagonist, inhibits development of atherosclerosis in rabbits. Atherosclerosis. 2005;183:65\u201373.","journal-title":"Atherosclerosis"},{"key":"245_CR7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0090-6980(83)90099-0","volume":"26","author":"RR Gorman","year":"1983","unstructured":"Gorman RR, Johnson RA, Spilman CH, Aiken JW. Inhibition of platelet thromboxane A2 synthase activity by sodium 5-(3\u2019-pyridinylmethyl)benzofuran-2-carboxylate. Prostaglandins. 1983;26:325\u201342.","journal-title":"Prostaglandins"},{"key":"245_CR8","doi-asserted-by":"crossref","first-page":"E4","DOI":"10.1067\/mhj.2002.121806","volume":"143","author":"D Langleben","year":"2002","unstructured":"Langleben D, Christman BW, Barst RJ, Dias VC, Galie N, Higenbottam TW, et al. Effects of the thromboxane synthetase inhibitor and receptor antagonist terbogrel in patients with primary pulmonary hypertension. Am Heart J. 2002;143:E4.","journal-title":"Am Heart J"},{"key":"245_CR9","first-page":"519","volume":"68","author":"ZM Nofal","year":"2011","unstructured":"Nofal ZM, Soliman EA, Abd El-Karim SS, El Zahar MI, Srour AM, Sethumadhavan S, et al. Novel benzimidazole derivatives as expected anticancer agents. Acta Pol Pharm. 2011;68:519\u201334.","journal-title":"Acta Pol Pharm"},{"key":"245_CR10","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/j.bmcl.2007.12.023","volume":"18","author":"C Congiu","year":"2008","unstructured":"Congiu C, Cocco MT, Onnis V. Design, synthesis, and in vitro antitumor activity of new 1,4-diarylimidazole-2-ones and their 2-thione analogues. Bioorg Med Chem Lett. 2008;18:989\u201393.","journal-title":"Bioorg Med Chem Lett"},{"key":"245_CR11","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/j.bmcl.2007.11.118","volume":"18","author":"L Nagarapu","year":"2008","unstructured":"Nagarapu L, Satyender A, Rajashaker B, Srinivas K, Rupa Rani P, Radhika K, et al. Synthesis and antimicrobial activity of novel C-linked imidazole glycoconjugates. Bioorg Med Chem Lett. 2008;18:1167\u201371.","journal-title":"Bioorg Med Chem Lett"},{"key":"245_CR12","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.ejmech.2008.01.006","volume":"44","author":"K Bhandari","year":"2009","unstructured":"Bhandari K, Srinivas N, Shiva Keshava GB, Shukla PK. Tetrahydronaphthyl azole oxime ethers: the conformationally rigid analogues of oxiconazole as antibacterials. Eur J Med Chem. 2009;44:437\u201347.","journal-title":"Eur J Med Chem"},{"key":"245_CR13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.bmcl.2007.10.111","volume":"18","author":"S Emami","year":"2008","unstructured":"Emami S, Foroumadi A, Falahati M, Lotfali E, Rajabalian S, Ebrahimi SA, et al. 2-Hydroxyphenacyl azoles and related azolium derivatives as antifungal agents. Bioorg Med Chem Lett. 2008;18:141\u20136.","journal-title":"Bioorg Med Chem Lett"},{"key":"245_CR14","doi-asserted-by":"crossref","first-page":"649","DOI":"10.2174\/1389557043403684","volume":"4","author":"JM Dogne","year":"2004","unstructured":"Dogne JM, Hanson J, de Leval X, Masereel B, Kolh P, Pirotte B. New developments on thromboxane modulators. Mini Rev Med Chem. 2004;4:649\u201357.","journal-title":"Mini Rev Med Chem"},{"key":"245_CR15","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s00210-010-0505-x","volume":"381","author":"HL Kuo","year":"2010","unstructured":"Kuo HL, Lien JC, Chung CH, Chang CH, Lo SC, Tsai IC, et al. NP-184[2-(5-methyl-2-furyl) benzimidazole], a novel orally active antithrombotic agent with dual antiplatelet and anticoagulant activities. Naunyn Schmiedebergs Arch Pharmacol. 2010;381:495\u2013505.","journal-title":"Naunyn Schmiedebergs Arch Pharmacol"},{"key":"245_CR16","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1055\/s-0037-1615759","volume":"85","author":"JM Herbert","year":"2001","unstructured":"Herbert JM, Herault JP, Bernat A, Savi P, Schaeffer P, Driguez PA, et al. SR123781A, a synthetic heparin mimetic. Thromb Haemost. 2001;85:852\u201360.","journal-title":"Thromb Haemost"},{"key":"245_CR17","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1046\/j.1538-7836.2003.00360.x","volume":"1","author":"P Savi","year":"2003","unstructured":"Savi P, Pflieger AM, Herault JP, Michaux C, Duchaussoy P, Petitou M, et al. Comparative effects of two synthetic oligosaccharides on platelet activation induced by plasma from HIT patients. J Thromb Haemost. 2003;1:2008\u201313.","journal-title":"J Thromb Haemost"},{"key":"245_CR18","doi-asserted-by":"crossref","first-page":"3848","DOI":"10.1021\/jo800234x","volume":"73","author":"C Lin","year":"2008","unstructured":"Lin C, Lai PT, Liao SK, Hung WT, Yang WB, Fang JM. Using molecular iodine in direct oxidative condensation of aldoses with diamines: an improved synthesis of aldo-benzimidazoles and aldo-naphthimidazoles for carbohydrate analysis. J Org Chem. 2008;73:3848\u201353.","journal-title":"J Org Chem"},{"key":"245_CR19","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1055\/s-0037-1613907","volume":"83","author":"JR Sheu","year":"2000","unstructured":"Sheu JR, Lee CR, Lin CH, Hsiao G, Ko WC, Chen YC, et al. Mechanisms involved in the antiplatelet activity of Staphylococcus aureus lipoteichoic acid in human platelets. Thromb Haemost. 2000;83:777\u201384.","journal-title":"Thromb Haemost"},{"key":"245_CR20","volume-title":"The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 5th edition","author":"M Westerfield","year":"2007","unstructured":"Westerfield M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 5th edition. Eugene: University of Oregon Press; 2007."},{"key":"245_CR21","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25\u00a0years of image analysis. Nat Methods. 2012;9:671\u20135.","journal-title":"Nat Methods"},{"key":"245_CR22","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1067\/mlc.2001.117406","volume":"138","author":"B Jilma","year":"2001","unstructured":"Jilma B. Platelet function analyzer (PFA-100): a tool to quantify congenital or acquired platelet dysfunction. J Lab Clin Med. 2001;138:152\u201363.","journal-title":"J Lab Clin Med"},{"key":"245_CR23","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1161\/01.ATV.0000174798.25085.d6","volume":"25","author":"G Hsiao","year":"2005","unstructured":"Hsiao G, Lin KH, Chang Y, Chen TL, Tzu NH, Chou DS, et al. Protective mechanisms of inosine in platelet activation and cerebral ischemic damage. Arterioscler Thromb Vasc Biol. 2005;25:1998\u20132004.","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"245_CR24","first-page":"223","volume":"2","author":"H Nishino","year":"1994","unstructured":"Nishino H, Czurko A, Onizuka K, Fukuda A, Hida H, Ungsuparkorn C, et al. Neuronal damage following transient cerebral ischemia and its restoration by neural transplant. Neurobiology (Bp). 1994;2:223\u201334.","journal-title":"Neurobiology (Bp)"},{"key":"245_CR25","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1146\/annurev.biochem.66.1.475","volume":"66","author":"WD Singer","year":"1997","unstructured":"Singer WD, Brown HA, Sternweis PC. Regulation of eukaryotic phosphatidylinositol-specific phospholipase C and phospholipase D. Annu Rev Biochem. 1997;66:475\u2013509.","journal-title":"Annu Rev Biochem"},{"key":"245_CR26","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.amjmed.2004.01.014","volume":"116","author":"MD Schleinitz","year":"2004","unstructured":"Schleinitz MD, Weiss JP, Owens DK. Clopidogrel versus aspirin for secondary prophylaxis of vascular events: a cost-effectiveness analysis. Am J Med. 2004;116:797\u2013806.","journal-title":"Am J Med"},{"key":"245_CR27","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1046\/j.1538-7836.2003.00267.x","volume":"1","author":"SP Jackson","year":"2003","unstructured":"Jackson SP, Nesbitt WS, Kulkarni S. Signaling events underlying thrombus formation. J Thromb Haemost. 2003;1:1602\u201312.","journal-title":"J Thromb Haemost"},{"key":"245_CR28","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1161\/01.ATV.0000251995.73307.2d","volume":"27","author":"K Hirano","year":"2007","unstructured":"Hirano K. The roles of proteinase-activated receptors in the vascular physiology and pathophysiology. Arterioscler Thromb Vasc Biol. 2007;27:27\u201336.","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"245_CR29","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1172\/JCI6042","volume":"103","author":"ML Kahn","year":"1999","unstructured":"Kahn ML, Nakanishi-Matsui M, Shapiro MJ, Ishihara H, Coughlin SR. Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin. J Clin Invest. 1999;103:879\u201387.","journal-title":"J Clin Invest"},{"key":"245_CR30","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1423-0127-20-4","volume":"20","author":"Y Chang","year":"2013","unstructured":"Chang Y, Huang SK, Lu WJ, Chung CL, Chen WL, Lu SH, et al. Brazilin isolated from Caesalpinia sappan L. acts as a novel collagen receptor agonist in human platelets. J Biomed Sci. 2013;20:4.","journal-title":"J Biomed Sci"},{"key":"245_CR31","doi-asserted-by":"crossref","first-page":"3652","DOI":"10.1182\/blood-2003-04-1323","volume":"102","author":"L He","year":"2003","unstructured":"He L, Pappan LK, Grenache DG, Li Z, Tollefsen DM, Santoro SA, et al. The contributions of the alpha 2 beta 1 integrin to vascular thrombosis in vivo. Blood. 2003;102:3652\u20137.","journal-title":"Blood"},{"key":"245_CR32","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1182\/blood-2002-12-3882","volume":"102","author":"B Nieswandt","year":"2003","unstructured":"Nieswandt B, Watson SP. Platelet-collagen interaction: is GPVI the central receptor? Blood. 2003;102:449\u201361.","journal-title":"Blood"},{"key":"245_CR33","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.phrs.2007.04.013","volume":"55","author":"A Pascale","year":"2007","unstructured":"Pascale A, Amadio M, Govoni S, Battaini F. The aging brain, a key target for the future: the protein kinase C involvement. Pharmacol Res. 2007;55:560\u20139.","journal-title":"Pharmacol Res"},{"key":"245_CR34","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1182\/blood-2007-02-075432","volume":"110","author":"A Ragab","year":"2007","unstructured":"Ragab A, Severin S, Gratacap MP, Aguado E, Malissen M, Jandrot-Perrus M, et al. Roles of the C-terminal tyrosine residues of LAT in GPVI-induced platelet activation: insights into the mechanism of PLC gamma 2 activation. Blood. 2007;110:2466\u201374.","journal-title":"Blood"},{"key":"245_CR35","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/35065000","volume":"410","author":"L Chang","year":"2001","unstructured":"Chang L, Karin M. Mammalian MAP kinase signalling cascades. Nature. 2001;410:37\u201340.","journal-title":"Nature"},{"key":"245_CR36","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1136\/gut.52.1.144","volume":"52","author":"DW Hommes","year":"2003","unstructured":"Hommes DW, Peppelenbosch MP, van Deventer SJ. Mitogen activated protein (MAP) kinase signal transduction pathways and novel anti-inflammatory targets. Gut. 2003;52:144\u201351.","journal-title":"Gut"},{"key":"245_CR37","doi-asserted-by":"crossref","first-page":"3800","DOI":"10.1182\/blood.V94.11.3800","volume":"94","author":"F Bugaud","year":"1999","unstructured":"Bugaud F, Nadal-Wollbold F, Levy-Toledano S, Rosa JP, Bryckaert M. Regulation of c-jun-NH2 terminal kinase and extracellular-signal regulated kinase in human platelets. Blood. 1999;94:3800\u20135.","journal-title":"Blood"},{"key":"245_CR38","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1111\/j.1538-7836.2008.03169.x","volume":"6","author":"F Adam","year":"2008","unstructured":"Adam F, Kauskot A, Rosa JP, Bryckaert M. Mitogen-activated protein kinases in hemostasis and thrombosis. J Thromb Haemost. 2008;6:2007\u201316.","journal-title":"J Thromb Haemost"},{"key":"245_CR39","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/S0891-5849(03)00386-1","volume":"35","author":"L Coulon","year":"2003","unstructured":"Coulon L, Calzada C, Moulin P, Vericel E, Lagarde M. Activation of p38 mitogen-activated protein kinase\/cytosolic phospholipase A2 cascade in hydroperoxide-stressed platelets. Free Radic Biol Med. 2003;35:616\u201325.","journal-title":"Free Radic Biol Med"},{"key":"245_CR40","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/0092-8674(95)90534-0","volume":"81","author":"TF Franke","year":"1995","unstructured":"Franke TF, Yang SI, Chan TO, Datta K, Kazlauskas A, Morrison DK, et al. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell. 1995;81:727\u201336.","journal-title":"Cell"},{"key":"245_CR41","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1182\/blood-2003-10-3428","volume":"104","author":"J Chen","year":"2004","unstructured":"Chen J, De S, Damron DS, Chen WS, Hay N, Byzova TV. Impaired platelet responses to thrombin and collagen in AKT-1-deficient mice. Blood. 2004;104:1703\u201310.","journal-title":"Blood"},{"key":"245_CR42","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1172\/JCI200420267","volume":"113","author":"D Woulfe","year":"2004","unstructured":"Woulfe D, Jiang H, Morgans A, Monks R, Birnbaum M, Brass LF. Defects in secretion, aggregation, and thrombus formation in platelets from mice lacking Akt2. J Clin Invest. 2004;113:441\u201350.","journal-title":"J Clin Invest"},{"key":"245_CR43","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1007\/s00109-011-0800-0","volume":"89","author":"WJ Lu","year":"2011","unstructured":"Lu WJ, Lee JJ, Chou DS, Jayakumar T, Fong TH, Hsiao G, et al. A novel role of andrographolide, an NF-kappa B inhibitor, on inhibition of platelet activation: the pivotal mechanisms of endothelial nitric oxide synthase\/cyclic GMP. J Mol Med (Berl). 2011;89:1261\u201373.","journal-title":"J Mol Med (Berl)"},{"key":"245_CR44","doi-asserted-by":"crossref","first-page":"e1775","DOI":"10.1038\/cddis.2015.146","volume":"6","author":"P Jie","year":"2015","unstructured":"Jie P, Hong Z, Tian Y, Li Y, Lin L, Zhou L, et al. Activation of transient receptor potential vanilloid 4 induces apoptosis in hippocampus through downregulating PI3K\/Akt and upregulating p38 MAPK signaling pathways. Cell Death Dis. 2015;6:e1775.","journal-title":"Cell Death Dis"}],"container-title":["Journal of Biomedical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12929-016-0245-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T15:55:47Z","timestamp":1600185347000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.jbiomedsci.com\/content\/23\/1\/26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,17]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["245"],"URL":"https:\/\/doi.org\/10.1186\/s12929-016-0245-4","relation":{},"ISSN":["1423-0127"],"issn-type":[{"value":"1423-0127","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,17]]},"article-number":"26"}}