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Previous research has emphasized the significant role of <jats:italic>F. nucleatum<\/jats:italic> in the occurrence of CRC. However, the impact of <jats:italic>F. nucleatum<\/jats:italic> on CRC liver metastasis has not been well understood.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The effects of <jats:italic>F. nucleatum<\/jats:italic> on metastasis ability of CRC cell were evaluated in vitro were examined by wound-healing assay and transwell assay. The mouse model of CRC liver metastasis was constructed by spleen injection, and the degree of liver metastasis was assessed by in vivo bioluminescence imaging. The gene expression changes in CRC cells after co-culture with <jats:italic>F. nucleatum<\/jats:italic> was analyzed through transcriptome sequencing. qRT-PCR and Western Blot assays were performed to validate the expression of related genes and proteins.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The metastasis ability of CRC cells was significantly enhanced after co-culture with <jats:italic>F. nucleatum<\/jats:italic> in vitro. In the mouse model, <jats:italic>F. nucleatum<\/jats:italic> also promoted the development of liver metastasis in CRC. Mechanistically, <jats:italic>F. nucleatum<\/jats:italic> infection increased the expression of IL-8 by downregulated the level of miR-5692a, a regulatory microRNA of IL-8. This led to the activation of the ERK pathway and resulted in the epithelial-mesenchymal transition (EMT) of CRC cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our results suggest that <jats:italic>F. nucleatum<\/jats:italic> promotes CRC liver metastasis by inducing epithelial-mesenchymal transition through the miR-5692a\/IL-8 axis. These findings provide new insights for the prevention and treatment of colorectal cancer liver metastasis.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12929-024-01097-4","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T04:19:29Z","timestamp":1736137169000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Fusobacterium nucleatum promotes colorectal cancer liver metastasis via miR-5692a\/IL-8 axis by inducing epithelial-mesenchymal transition"],"prefix":"10.1186","volume":"32","author":[{"given":"Yulong","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bili","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiheng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aifeina","family":"Aili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-5388","authenticated-orcid":false,"given":"Xianglin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"issue":"1","key":"1097_CR1","doi-asserted-by":"publisher","first-page":"17","DOI":"10.3322\/caac.21763","volume":"73","author":"RL Siegel","year":"2023","unstructured":"Siegel RL, Miller KD, Wagle NS, Jemal A. 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