{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:21:34Z","timestamp":1790767294309,"version":"4.1.0"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T00:00:00Z","timestamp":1564963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T00:00:00Z","timestamp":1564963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2017M620133"],"award-info":[{"award-number":["2017M620133"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701498"],"award-info":[{"award-number":["81701498"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Respir Res"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s12931-019-1148-1","type":"journal-article","created":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T13:03:03Z","timestamp":1565010183000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Identification of genetic factors underlying persistent pulmonary hypertension of newborns in a cohort of Chinese neonates"],"prefix":"10.1186","volume":"20","author":[{"given":"Xu","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinran","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,8,5]]},"reference":[{"key":"1148_CR1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1542\/peds.105.1.14","volume":"105","author":"MC Walsh-Sukys","year":"2000","unstructured":"Walsh-Sukys MC, Tyson JE, Wright LL, Bauer CR, Korones SB, Stevenson DK, Verter J, Stoll BJ, Lemons JA, Papile LA, et al. Persistent pulmonary hypertension of the newborn in the era before nitric oxide: practice variation and outcomes. Pediatrics. 2000;105:14\u201320.","journal-title":"Pediatrics"},{"issue":"1","key":"1148_CR2","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.pedneo.2016.09.011","volume":"59","author":"Mei-Yin Lai","year":"2018","unstructured":"Lai MY, Chu SM, Lakshminrusimha S, Lin HC. Beyond the inhaled nitric oxide in persistent pulmonary hypertension of the newborn. Pediatr Neonatol. 2018;59(1):15-23. \n                    https:\/\/doi.org\/10.1016\/j.pedneo.2016.09.011\n                    \n                  .","journal-title":"Pediatrics & Neonatology"},{"key":"1148_CR3","doi-asserted-by":"publisher","first-page":"1832","DOI":"10.1056\/NEJM200106143442404","volume":"344","author":"DL Pearson","year":"2001","unstructured":"Pearson DL, Dawling S, Walsh WF, Haines JL, Christman BW, Bazyk A, Scott N, Summar ML. Neonatal pulmonary hypertension--urea-cycle intermediates, nitric oxide production, and carbamoyl-phosphate synthetase function. N Engl J Med. 2001;344:1832\u20138.","journal-title":"N Engl J Med"},{"key":"1148_CR4","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.jpeds.2007.05.054","volume":"151","author":"AM Kunig","year":"2007","unstructured":"Kunig AM, Parker TA, Nogee LM, Abman SH, Kinsella JP. ABCA3 deficiency presenting as persistent pulmonary hypertension of the newborn. J Pediatr. 2007;151:322\u20134.","journal-title":"J Pediatr"},{"key":"1148_CR5","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1038\/pr.2011.24","volume":"71","author":"HM Byers","year":"2012","unstructured":"Byers HM, Dagle JM, Klein JM, Ryckman KK, McDonald EL, Murray JC, Borowski KS. Variations in CRHR1 are associated with persistent pulmonary hypertension of the newborn. Pediatr Res. 2012;71:162\u20137.","journal-title":"Pediatr Res"},{"key":"1148_CR6","doi-asserted-by":"publisher","first-page":"29877","DOI":"10.1038\/srep29877","volume":"6","author":"M Mei","year":"2016","unstructured":"Mei M, Cheng G, Sun B, Yang L, Wang H, Sun J, Zhou W. EDN1 gene variant is associated with neonatal persistent pulmonary hypertension. Sci Rep. 2016;6:29877.","journal-title":"Sci Rep"},{"key":"1148_CR7","doi-asserted-by":"publisher","first-page":"2037","DOI":"10.1161\/CIR.0000000000000329","volume":"132","author":"SH Abman","year":"2015","unstructured":"Abman SH, Hansmann G, Archer SL, Ivy DD, Adatia I, Chung WK, Hanna BD, Rosenzweig EB, Raj JU, Cornfield D, et al. Pediatric pulmonary hypertension: guidelines from the American Heart Association and American Thoracic Society. Circulation. 2015;132:2037\u201399.","journal-title":"Circulation"},{"key":"1148_CR8","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1002\/humu.9398","volume":"27","author":"MA Aldred","year":"2006","unstructured":"Aldred MA, Vijayakrishnan J, James V, Soubrier F, Gomez-Sanchez MA, Martensson G, Galie N, Manes A, Corris P, Simonneau G, et al. BMPR2 gene rearrangements account for a significant proportion of mutations in familial and idiopathic pulmonary arterial hypertension. Hum Mutat. 2006;27:212\u20133.","journal-title":"Hum Mutat"},{"key":"1148_CR9","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1136\/jmg.2008.062703","volume":"46","author":"M Shintani","year":"2009","unstructured":"Shintani M, Yagi H, Nakayama T, Saji T, Matsuoka R. A new nonsense mutation of SMAD8 associated with pulmonary arterial hypertension. J Med Genet. 2009;46:331\u20137.","journal-title":"J Med Genet"},{"key":"1148_CR10","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1161\/CIRCGENETICS.111.961888","volume":"5","author":"ED Austin","year":"2012","unstructured":"Austin ED, Ma L, LeDuc C, Berman Rosenzweig E, Borczuk A, Phillips JA 3rd, Palomero T, Sumazin P, Kim HR, Talati MH, et al. Whole exome sequencing to identify a novel gene (caveolin-1) associated with human pulmonary arterial hypertension. Circ Cardiovasc Genet. 2012;5:336\u201343.","journal-title":"Circ Cardiovasc Genet"},{"key":"1148_CR11","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1056\/NEJMoa1211097","volume":"369","author":"L Ma","year":"2013","unstructured":"Ma L, Roman-Campos D, Austin ED, Eyries M, Sampson KS, Soubrier F, Germain M, Tregouet DA, Borczuk A, Rosenzweig EB, et al. A novel channelopathy in pulmonary arterial hypertension. N Engl J Med. 2013;369:351\u201361.","journal-title":"N Engl J Med"},{"key":"1148_CR12","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1136\/jmg.40.12.865","volume":"40","author":"RE Harrison","year":"2003","unstructured":"Harrison RE, Flanagan JA, Sankelo M, Abdalla SA, Rowell J, Machado RD, Elliott CG, Robbins IM, Olschewski H, McLaughlin V, et al. Molecular and functional analysis identifies ALK-1 as the predominant cause of pulmonary hypertension related to hereditary haemorrhagic telangiectasia. J Med Genet. 2003;40:865\u201371.","journal-title":"J Med Genet"},{"key":"1148_CR13","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1136\/thx.2003.11890","volume":"59","author":"A Chaouat","year":"2004","unstructured":"Chaouat A, Coulet F, Favre C, Simonneau G, Weitzenblum E, Soubrier F, Humbert M. Endoglin germline mutation in a patient with hereditary haemorrhagic telangiectasia and dexfenfluramine associated pulmonary arterial hypertension. Thorax. 2004;59:446\u20138.","journal-title":"Thorax"},{"key":"1148_CR14","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1542\/peds.107.3.519","volume":"107","author":"MA Alano","year":"2001","unstructured":"Alano MA, Ngougmna E, Ostrea EM Jr, Konduri GG. Analysis of nonsteroidal antiinflammatory drugs in meconium and its relation to persistent pulmonary hypertension of the newborn. Pediatrics. 2001;107:519\u201323.","journal-title":"Pediatrics"},{"key":"1148_CR15","doi-asserted-by":"publisher","first-page":"e164","DOI":"10.1093\/nar\/gkq603","volume":"38","author":"K Wang","year":"2010","unstructured":"Wang K, Li M, Hakonarson H. ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res. 2010;38:e164.","journal-title":"Nucleic Acids Res"},{"key":"1148_CR16","doi-asserted-by":"publisher","first-page":"2069","DOI":"10.1093\/bioinformatics\/btq330","volume":"26","author":"W McLaren","year":"2010","unstructured":"McLaren W, Pritchard B, Rios D, Chen Y, Flicek P, Cunningham F. Deriving the consequences of genomic variants with the Ensembl API and SNP effect predictor. Bioinformatics. 2010;26:2069\u201370.","journal-title":"Bioinformatics"},{"key":"1148_CR17","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1038\/nprot.2009.86","volume":"4","author":"P Kumar","year":"2009","unstructured":"Kumar P, Henikoff S, Ng PC. Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm. Nat Protoc. 2009;4:1073\u201381.","journal-title":"Nat Protoc"},{"key":"1148_CR18","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1038\/nmeth0410-248","volume":"7","author":"IA Adzhubei","year":"2010","unstructured":"Adzhubei IA, Schmidt S, Peshkin L, Ramensky VE, Gerasimova A, Bork P, Kondrashov AS, Sunyaev SR. A method and server for predicting damaging missense mutations. Nat Methods. 2010;7:248\u20139.","journal-title":"Nat Methods"},{"key":"1148_CR19","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1038\/nmeth0810-575","volume":"7","author":"JM Schwarz","year":"2010","unstructured":"Schwarz JM, Rodelsperger C, Schuelke M, Seelow D. MutationTaster evaluates disease-causing potential of sequence alterations. Nat Methods. 2010;7:575\u20136.","journal-title":"Nat Methods"},{"key":"1148_CR20","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1038\/gim.2015.30","volume":"17","author":"S Richards","year":"2015","unstructured":"Richards S, Aziz N, Bale S, Bick D, Das S, Gastier-Foster J, Grody WW, Hegde M, Lyon E, Spector E, et al. Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med. 2015;17:405\u201324.","journal-title":"Genet Med"},{"key":"1148_CR21","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.ajhg.2011.05.029","volume":"89","author":"MC Wu","year":"2011","unstructured":"Wu MC, Lee S, Cai T, Li Y, Boehnke M, Lin X. Rare-variant association testing for sequencing data with the sequence kernel association test. Am J Hum Genet. 2011;89:82\u201393.","journal-title":"Am J Hum Genet"},{"key":"1148_CR22","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1086\/316947","volume":"68","author":"RD Machado","year":"2001","unstructured":"Machado RD, Pauciulo MW, Thomson JR, Lane KB, Morgan NV, Wheeler L, Phillips JA 3rd, Newman J, Williams D, Galie N, et al. BMPR2 haploinsufficiency as the inherited molecular mechanism for primary pulmonary hypertension. Am J Hum Genet. 2001;68:92\u2013102.","journal-title":"Am J Hum Genet"},{"key":"1148_CR23","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1016\/j.recesp.2016.03.031","volume":"69","author":"P Navas","year":"2016","unstructured":"Navas P, Tenorio J, Quezada CA, Barrios E, Gordo G, Arias P, Lopez Meseguer M, Santos-Lozano A, Palomino Doza J, Lapunzina P, Escribano Subias P. Molecular analysis of BMPR2, TBX4, and KCNK3 and genotype-phenotype correlations in Spanish patients and families with idiopathic and hereditary pulmonary arterial hypertension. Rev Esp Cardiol (Engl Ed). 2016;69:1011\u20139.","journal-title":"Rev Esp Cardiol (Engl Ed)"},{"key":"1148_CR24","doi-asserted-by":"publisher","first-page":"6851","DOI":"10.1021\/bi00481a014","volume":"29","author":"LE Gentry","year":"1990","unstructured":"Gentry LE, Nash BW. The pro domain of pre-pro-transforming growth factor beta 1 when independently expressed is a functional binding protein for the mature growth factor. Biochemistry. 1990;29:6851\u20137.","journal-title":"Biochemistry"},{"key":"1148_CR25","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.siny.2017.03.004","volume":"22","author":"M Fuloria","year":"2017","unstructured":"Fuloria M, Aschner JL. Persistent pulmonary hypertension of the newborn. Semin Fetal Neonatal Med. 2017;22:220\u20136.","journal-title":"Semin Fetal Neonatal Med"},{"key":"1148_CR26","doi-asserted-by":"publisher","first-page":"L993","DOI":"10.1152\/ajplung.00191.2005","volume":"291","author":"I Fantozzi","year":"2006","unstructured":"Fantozzi I, Platoshyn O, Wong AH, Zhang S, Remillard CV, Furtado MR, Petrauskene OV, Yuan JX. Bone morphogenetic protein-2 upregulates expression and function of voltage-gated K+ channels in human pulmonary artery smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 2006;291:L993\u20131004.","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"1148_CR27","doi-asserted-by":"publisher","first-page":"e001887","DOI":"10.1161\/CIRCGEN.117.001887","volume":"11","author":"N Zhu","year":"2018","unstructured":"Zhu N, Gonzaga-Jauregui C, Welch CL, Ma L, Qi H, King AK, Krishnan U, Rosenzweig EB, Ivy DD, Austin ED, et al. Exome sequencing in children with pulmonary arterial hypertension demonstrates differences compared with adults. Circ Genom Precis Med. 2018;11:e001887.","journal-title":"Circ Genom Precis Med"},{"key":"1148_CR28","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1183\/13993003.00211-2016","volume":"48","author":"M Levy","year":"2016","unstructured":"Levy M, Eyries M, Szezepanski I, Ladouceur M, Nadaud S, Bonnet D, Soubrier F. Genetic analyses in a cohort of children with pulmonary hypertension. Eur Respir J. 2016;48:1118\u201326.","journal-title":"Eur Respir J"},{"key":"1148_CR29","doi-asserted-by":"publisher","first-page":"e1002866","DOI":"10.1371\/journal.pgen.1002866","volume":"8","author":"R Arora","year":"2012","unstructured":"Arora R, Metzger RJ, Papaioannou VE. Multiple roles and interactions of Tbx4 and Tbx5 in development of the respiratory system. PLoS Genet. 2012;8:e1002866.","journal-title":"PLoS Genet"},{"key":"1148_CR30","first-page":"99","volume":"21","author":"W Jachec","year":"2008","unstructured":"Jachec W, Foremny A, Domal-Kwiatkowska D, Smolik S, Tomasik A, Mazurek U, Wodniecki J. Expression of TGF-beta1 and its receptor genes (TbetaR I, TbetaR II, and TbetaR III-betaglycan) in peripheral blood leucocytes in patients with idiopathic pulmonary arterial hypertension and Eisenmenger's syndrome. Int J Mol Med. 2008;21:99\u2013107.","journal-title":"Int J Mol Med"},{"key":"1148_CR31","doi-asserted-by":"publisher","first-page":"7596","DOI":"10.1038\/srep07596","volume":"4","author":"S Tsukamoto","year":"2014","unstructured":"Tsukamoto S, Mizuta T, Fujimoto M, Ohte S, Osawa K, Miyamoto A, Yoneyama K, Murata E, Machiya A, Jimi E, et al. Smad9 is a new type of transcriptional regulator in bone morphogenetic protein signaling. Sci Rep. 2014;4:7596.","journal-title":"Sci Rep"},{"key":"1148_CR32","doi-asserted-by":"publisher","first-page":"C1837","DOI":"10.1152\/ajpcell.00405.2006","volume":"292","author":"CV Remillard","year":"2007","unstructured":"Remillard CV, Tigno DD, Platoshyn O, Burg ED, Brevnova EE, Conger D, Nicholson A, Rana BK, Channick RN, Rubin LJ, et al. Function of Kv1.5 channels and genetic variations of KCNA5 in patients with idiopathic pulmonary arterial hypertension. Am J Physiol Cell Physiol. 2007;292:C1837\u201353.","journal-title":"Am J Physiol Cell Physiol"},{"key":"1148_CR33","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1016\/j.pharmthera.2006.05.013","volume":"112","author":"A Dietrich","year":"2006","unstructured":"Dietrich A, Chubanov V, Kalwa H, Rost BR, Gudermann T. Cation channels of the transient receptor potential superfamily: their role in physiological and pathophysiological processes of smooth muscle cells. Pharmacol Ther. 2006;112:744\u201360.","journal-title":"Pharmacol Ther"},{"key":"1148_CR34","doi-asserted-by":"publisher","first-page":"2313","DOI":"10.1161\/CIRCULATIONAHA.108.782458","volume":"119","author":"Y Yu","year":"2009","unstructured":"Yu Y, Keller SH, Remillard CV, Safrina O, Nicholson A, Zhang SL, Jiang W, Vangala N, Landsberg JW, Wang JY, et al. A functional single-nucleotide polymorphism in the TRPC6 gene promoter associated with idiopathic pulmonary arterial hypertension. Circulation. 2009;119:2313\u201322.","journal-title":"Circulation"},{"key":"1148_CR35","doi-asserted-by":"publisher","first-page":"2675","DOI":"10.1172\/JCI117281","volume":"93","author":"JR Fineman","year":"1994","unstructured":"Fineman JR, Wong J, Morin FC 3rd, Wild LM, Soifer SJ. Chronic nitric oxide inhibition in utero produces persistent pulmonary hypertension in newborn lambs. J Clin Invest. 1994;93:2675\u201383.","journal-title":"J Clin Invest"},{"key":"1148_CR36","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1038\/pr.2017.143","volume":"83","author":"DC Kaluarachchi","year":"2018","unstructured":"Kaluarachchi DC, Smith CJ, Klein JM, Murray JC, Dagle JM, Ryckman KK. Polymorphisms in urea cycle enzyme genes are associated with persistent pulmonary hypertension of the newborn. Pediatr Res. 2018;83:142\u20137.","journal-title":"Pediatr Res"},{"key":"1148_CR37","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1002\/mgg3.58","volume":"2","author":"A Chida","year":"2014","unstructured":"Chida A, Shintani M, Matsushita Y, Sato H, Eitoku T, Nakayama T, Furutani Y, Hayama E, Kawamura Y, Inai K, et al. Mutations of NOTCH3 in childhood pulmonary arterial hypertension. Mol Genet Genomic Med. 2014;2:229\u201339.","journal-title":"Mol Genet Genomic Med"}],"container-title":["Respiratory Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12931-019-1148-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12931-019-1148-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12931-019-1148-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T23:15:14Z","timestamp":1596496514000},"score":1,"resource":{"primary":{"URL":"https:\/\/respiratory-research.biomedcentral.com\/articles\/10.1186\/s12931-019-1148-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,5]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["1148"],"URL":"https:\/\/doi.org\/10.1186\/s12931-019-1148-1","relation":{},"ISSN":["1465-993X"],"issn-type":[{"value":"1465-993X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,5]]},"assertion":[{"value":"31 January 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 July 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 August 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All guardians of the subjects included in this study\u00a0were provided with\u00a0appropriate informed consent. This study was approved by the ethics committee of the Children\u2019s Hospital of Fudan University and adhered to the Declaration of Helsinki.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"174"}}