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We aimed at investigating the usefulness of measuring the proportion of T-cell receptor (TCR) CD4+ V\u03b12.3+ T-cells in BALF as an additive marker to CD4\/CD8-ratio to confirm the diagnosis.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>From a register consisting of 749 sarcoidosis patients [L\u00f6fgren\u2019s syndrome (LS) <jats:italic>n<\/jats:italic>\u2009=\u2009274, non-LS <jats:italic>n<\/jats:italic>\u2009=\u2009475] with information on V\u03b12.3+ T-cells, an expansion of CD4+ V\u03b12.3+ T-cells (CD4+ V\u03b12.3+ T cells &gt;\u200910.5% in BALF) was seen in 268 (36%). Controls were healthy volunteers (<jats:italic>n<\/jats:italic>\u2009=\u200969) and patients with other pulmonary conditions (<jats:italic>n<\/jats:italic>\u2009=\u200939), investigated because of suspicion of sarcoidosis.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>A proportion of CD4+ V\u03b12.3+ T-cells in BALF &gt;\u200910.5% was highly specific for sarcoidosis, with a specificity of 97% and with a sensitivity of 36% (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Receiver operating characteristic (ROC) curves show that testing for CD4+ V\u03b12.3+ T-cells in BALF was a more useable test in individuals with LS [area under the curve (AUC) 0.82, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001] compared to the whole patient group (AUC 0.64, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>In this study, we show that an increased proportion of CD4+ V\u03b12.3+ T-cells in BALF is highly specific for sarcoidosis. This suggests that this T-cell subset could be used as an additional tool to the CD4\/CD8-ratio to support the sarcoidosis diagnosis, particularly in patients with LS but also in patients with non-LS.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12931-020-1327-0","type":"journal-article","created":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T15:02:53Z","timestamp":1582902173000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Lung CD4+ V\u03b12.3+ T-cells in sarcoidosis cohorts with L\u00f6fgren\u2019s syndrome"],"prefix":"10.1186","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3411-8838","authenticated-orcid":false,"given":"Pernilla","family":"Darlington","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna","family":"Kullberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Eklund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Grunewald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,2,28]]},"reference":[{"issue":"12","key":"1327_CR1","doi-asserted-by":"publisher","first-page":"2699","DOI":"10.1183\/09031936.97.10122699","volume":"10","author":"U Costabel","year":"1997","unstructured":"Costabel U. 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