{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T19:13:39Z","timestamp":1771528419416,"version":"3.50.1"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T00:00:00Z","timestamp":1625443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T00:00:00Z","timestamp":1625443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["U54HD071594"],"award-info":[{"award-number":["U54HD071594"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Respir Res"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Neonatal intermittent hypoxia (IH) results in oxidative distress in preterm infants with immature antioxidant systems, contributing to lung injury. Coenzyme Q10 (CoQ10) and fish oil protect against oxidative injury. We tested the hypothesis that CoQ10 is more effective than fish oil for prevention of IH-induced lung injury in neonatal rats.\n<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Newborn rats were exposed to two clinically relevant IH paradigms at birth (P0): (1) 50% O<jats:sub>2<\/jats:sub> with brief hypoxia (12% O<jats:sub>2<\/jats:sub>); or (2) room air (RA) with brief hypoxia (12% O<jats:sub>2<\/jats:sub>), until P14 during which they were supplemented with daily oral CoQ10, fish oil, or olive oil from P0 to P14. Pups were studied at P14 or placed in RA until P21 with no further treatment. Lungs were assessed for histopathology and morphometry; biomarkers of oxidative stress and lipid peroxidation; and antioxidants.\n<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Of the two neonatal IH paradigms 21%\/12% O<jats:sub>2<\/jats:sub> IH resulted in the most severe outcomes, evidenced by histopathology and morphometry. CoQ10 was effective for preserving lung architecture and reduction of IH-induced oxidative stress biomarkers. In contrast, fish oil resulted in significant adverse outcomes including oversimplified alveoli, hemorrhage, reduced secondary crest formation and thickened septae. This was associated with elevated oxidants and antioxidants activities.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Data suggest that higher FiO<jats:sub>2<\/jats:sub> may be needed between IH episodes to curtail the damaging effects of IH, and to provide the lungs with necessary respite. The negative outcomes with fish oil supplementation suggest oxidative stress-induced lipid peroxidation.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12931-021-01786-w","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T08:10:58Z","timestamp":1625472658000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of coenzyme Q10 or fish oil for prevention of intermittent hypoxia-induced oxidative injury in neonatal rat lungs"],"prefix":"10.1186","volume":"22","author":[{"given":"Christina","family":"D\u2019Agrosa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles L.","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faisal","family":"Siddiqui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Deslouches","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Wadowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob V.","family":"Aranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4129-475X","authenticated-orcid":false,"given":"Kay D.","family":"Beharry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,7,5]]},"reference":[{"issue":"1","key":"1786_CR1","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1038\/s41572-019-0127-7","volume":"5","author":"B Th\u00e9baud","year":"2019","unstructured":"Th\u00e9baud B, Goss KN, Laughon M, Whitsett JA, Abman SH, Steinhorn RH, Aschner JL, Davis PG, McGrath-Morrow SA, Soll RF, Jobe AH. Bronchopulmonary dysplasia. Nat Rev Dis Primers. 2019;5(1):78.","journal-title":"Nat Rev Dis Primers"},{"key":"1786_CR2","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1038\/pr.2012.132","volume":"72","author":"JM Di Fiore","year":"2012","unstructured":"Di Fiore JM, Kaffashi F, Loparo K, Sattar A, Schluchter M, Foglyano R, Martin RJ, Wilson CG. The relationship between patterns of intermittent hypoxia and retinopathy of prematurity in preterm infants. Pediatr Res. 2012;72:606\u201312.","journal-title":"Pediatr Res"},{"key":"1786_CR3","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1203\/PDR.0b013e31817307ac","volume":"64","author":"RJ Coleman","year":"2008","unstructured":"Coleman RJ, Beharry KD, Brock RS, Abad-Santos P, Abad-Santos M, Modanlou HD. Effects of brief, clustered versus dispersed hypoxic episodes on systemic and ocular growth factors in a rat model of oxygen-induced retinopathy. Pediatr Res. 2008;64:50\u20135.","journal-title":"Pediatr Res"},{"key":"1786_CR4","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1038\/pr.2016.240","volume":"81","author":"AM Dylag","year":"2017","unstructured":"Dylag AM, Mayer CA, Raffay TM, Martin RJ, Jafri A, MacFarlane PM. 2017 Long-term effects of recurrent intermittent hypoxia and hyperoxia on respiratory system mechanics in neonatal mice. Pediatr Res. 2017;81:565\u201371.","journal-title":"Pediatr Res"},{"key":"1786_CR5","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1159\/000100086","volume":"92","author":"V Ratner","year":"2007","unstructured":"Ratner V, Kishkurno SV, Slinko SK, Sosunov SA, Sosunov AA, Polin RA, Ten VS. The contribution of intermittent hypoxemia to late neurological handicap in mice with hyperoxia induced lung injury. Neonatology. 2007;92:50\u20138.","journal-title":"Neonatology"},{"issue":"3","key":"1786_CR6","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1002\/bdra.23235","volume":"100","author":"EA Jensen","year":"2014","unstructured":"Jensen EA, Schmidt B. Epidemiology of bronchopulmonary dysplasia. Birth Defects Res A Clin Mol Teratol. 2014;100(3):145\u201357.","journal-title":"Birth Defects Res A Clin Mol Teratol"},{"issue":"5","key":"1786_CR7","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1038\/s41390-018-0167-9","volume":"84","author":"J Ibrahim","year":"2018","unstructured":"Ibrahim J, Bhandari V. The definition of bronchopulmonary dysplasia: an evolving dilemma. Pediatr Res. 2018;84(5):586\u20138.","journal-title":"Pediatr Res"},{"issue":"2","key":"1786_CR8","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1007\/s00408-018-0084-z","volume":"196","author":"JS Hwang","year":"2018","unstructured":"Hwang JS, Rehan VK. Recent advances in bronchopulmonary dysplasia: pathophysiology, prevention, and treatment. Lung. 2018;196(2):129\u201338.","journal-title":"Lung"},{"issue":"1","key":"1786_CR9","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S1084-2756(02)00194-X","volume":"8","author":"OD Saugstad","year":"2003","unstructured":"Saugstad OD. Bronchopulmonary dysplasia-oxidative stress and antioxidants. Semin Neonatol. 2003;8(1):39\u201349.","journal-title":"Semin Neonatol"},{"key":"1786_CR10","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.freeradbiomed.2019.03.035","volume":"142","author":"M Perez","year":"2019","unstructured":"Perez M, Robbins ME, Revhaug C, Saugstad OD. Oxygen radical disease in the newborn, revisited: oxidative stress and disease in the newborn period. Free Radic Biol Med. 2019;142:61\u201372.","journal-title":"Free Radic Biol Med"},{"issue":"5","key":"1786_CR11","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1203\/00006450-200005000-00014","volume":"47","author":"IH Buss","year":"2000","unstructured":"Buss IH, Darlow BA, Winterbourn CC. Elevated protein carbonyls and lipid peroxidation products correlating with myeloperoxidase in tracheal aspirates from premature infants. Pediatr Res. 2000;47(5):640\u20135.","journal-title":"Pediatr Res"},{"key":"1786_CR12","doi-asserted-by":"publisher","first-page":"30","DOI":"10.3389\/fped.2019.00030","volume":"7","author":"L Capasso","year":"2019","unstructured":"Capasso L, Vento G, Loddo C, Tirone C, Iavarone F, Raimondi F, Dani C, Fanos V. Oxidative stress and bronchopulmonary dysplasia: evidences from microbiomics, metabolomics, and proteomics. Front Pediatr. 2019;7:30.","journal-title":"Front Pediatr"},{"key":"1786_CR13","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.gene.2018.08.031","volume":"678","author":"J Wang","year":"2018","unstructured":"Wang J, Dong W. Oxidative stress and bronchopulmonary dysplasia. Gene. 2018;678:177\u201383.","journal-title":"Gene"},{"issue":"3","key":"1786_CR14","doi-asserted-by":"publisher","first-page":"109","DOI":"10.4103\/2249-4847.101683","volume":"1","author":"S Perrone","year":"2012","unstructured":"Perrone S, Tataranno ML, Buonocore G. Oxidative stress and bronchopulmonary dysplasia. J Clin Neonatol. 2012;1(3):109\u201314.","journal-title":"J Clin Neonatol"},{"key":"1786_CR15","doi-asserted-by":"publisher","first-page":"619","DOI":"10.2741\/4231","volume":"19","author":"J Garrido-Maraver","year":"2014","unstructured":"Garrido-Maraver J, Cordero MD, Oropesa-Avila M, Vega AF, de la Mata M, Pavon AD, Alcocer-Gomez E, Calero CP, Paz MV, Alanis M, de Lavera I, Cotan D, Sanchez-Alcazar JA. Clinical applications of coenzyme Q10. Front Biosci. 2014;19:619\u201333.","journal-title":"Front Biosci"},{"key":"1786_CR16","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1038\/ejcn.2010.98","volume":"64","author":"M Skouroliakou","year":"2010","unstructured":"Skouroliakou M, Konstantinou D, Koutri K, Kakavelaki C, Stathopoulou M, Antoniadi M, Xemelidis N, Kona V, Markantonis S. A double-blind, randomized clinical trial of the effect of omega-3 fatty acids on the oxidative stress of preterm neonates fed through parenteral nutrition. Eur J Clin Nutr. 2010;64:940\u20137.","journal-title":"Eur J Clin Nutr"},{"issue":"5","key":"1786_CR17","doi-asserted-by":"publisher","first-page":"430","DOI":"10.3390\/antiox9050430","volume":"9","author":"A Sad\u017eak","year":"2020","unstructured":"Sad\u017eak A, Mravljak J, Maltar-Strme\u010dki N, Arsov Z, Baranovi\u0107 G, Erceg I, Kriechbaum M, Strasser V, P\u0159ibyl J, \u0160egota S. The structural integrity of the model lipid membrane during induced lipid peroxidation: the Role of flavonols in the inhibition of lipid peroxidation. Antioxidants (Basel). 2020;9(5):430.","journal-title":"Antioxidants (Basel)"},{"issue":"4","key":"1786_CR18","doi-asserted-by":"publisher","first-page":"103","DOI":"10.3390\/antiox6040103","volume":"6","author":"KD Beharry","year":"2017","unstructured":"Beharry KD, Cai CL, Henry MM, Chowdhury S, Valencia GB, Aranda JV. Co-Enzyme Q10 and n-3 polyunsaturated fatty acid supplementation reverses intermittent hypoxia-induced growth restriction and improved antioxidant profiles in neonatal rats. Antioxidants (Basel). 2017;6(4):103.","journal-title":"Antioxidants (Basel)"},{"issue":"5","key":"1786_CR19","doi-asserted-by":"publisher","first-page":"E1337","DOI":"10.3390\/ijms19051337","volume":"19","author":"KD Beharry","year":"2018","unstructured":"Beharry KD, Cai CL, Skelton J, Siddiqui F, D\u2019Agrosa C, Calo J, Valencia GB, Aranda JV. Oxygen-induced retinopathy from recurrent intermittent hypoxia is not dependent on resolution with room air or oxygen, in neonatal rats. Int J Mol Sci. 2018;19(5):E1337.","journal-title":"Int J Mol Sci"},{"key":"1786_CR20","doi-asserted-by":"publisher","first-page":"160","DOI":"10.3390\/antiox7110160","volume":"7","author":"K Beharry","year":"2018","unstructured":"Beharry K, Cai C, Siddiqui F, Chowdhury S, D\u2019Agrosa C, Valencia G, Aranda JV. Comparative effects of coenzyme Q10 or n-3 polyunsaturated fatty acid supplementation on retinal angiogenesis in a rat model of oxygen-induced retinopathy. Antioxidants (Basel). 2018;7:160.","journal-title":"Antioxidants (Basel)"},{"key":"1786_CR21","doi-asserted-by":"publisher","first-page":"1980","DOI":"10.3390\/nu12071980","volume":"12","author":"KD Beharry","year":"2020","unstructured":"Beharry KD, Cai CL, Siddiqui F, D\u2019Agrosa C, Zangaladze A, Mustafa G, Qadri A, Duggan TJ, Aranda JV. Combination antioxidant\/NSAID therapies and oral\/topical ocular delivery modes for prevention of oxygen-induced retinopathy in a rat model. Nutrients. 2020;12:1980.","journal-title":"Nutrients"},{"issue":"1","key":"1786_CR22","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1002\/ar.1091800109","volume":"180","author":"PH Burri","year":"1974","unstructured":"Burri PH. The postnatal growth of the rat lung. 3. Morphology. Anat Rec. 1974;180(1):77\u201398.","journal-title":"Anat Rec"},{"issue":"1","key":"1786_CR23","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1152\/japplphysiol.01355.2013","volume":"117","author":"SA Tschanz","year":"2014","unstructured":"Tschanz SA, Salm LA, Roth-Kleiner M, Barr\u00e9 SF, Burri PH, Schittny JC. Rat lungs show a biphasic formation of new alveoli during postnatal development. J Appl Physiol. 2014;117(1):89\u201395.","journal-title":"J Appl Physiol"},{"key":"1786_CR24","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1080\/01926230490465874","volume":"32","author":"SA Bailey","year":"2004","unstructured":"Bailey SA, Zidell RH, Perry RW. Relationships between organ weight and body\/brain weight in the rat: what is the best analytical endpoint? Toxicol Pathol. 2004;32:448\u201366.","journal-title":"Toxicol Pathol"},{"key":"1786_CR25","doi-asserted-by":"crossref","unstructured":"Duggan TJ, Cai CL, Aranda JV, Beharry KD. Acute and chronic effects of intravitreal bevacizumab on lung biomarkers of angiogenesis in the rat exposed to neonatal intermittent hypoxia. Exp Lung Res. 2020:1\u201315.","DOI":"10.1080\/01902148.2020.1866712"},{"issue":"18","key":"1786_CR26","doi-asserted-by":"publisher","first-page":"10099","DOI":"10.1016\/S0021-9258(17)39218-9","volume":"260","author":"PE Starke","year":"1985","unstructured":"Starke PE, Farber JL. Ferric iron and superoxide ions are required for the killing of cultured hepatocytes by hydrogen peroxide. Evidence for the participation of hydroxyl radicals formed by an iron-catalyzed Haber-Weiss reaction. J Biol Chem. 1985;260(18):10099\u2013104.","journal-title":"J Biol Chem"},{"issue":"3","key":"1786_CR27","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/0165-1110(90)90014-3","volume":"238","author":"H Esterbauer","year":"1990","unstructured":"Esterbauer H, Eckl P, Ortner A. Possible mutagens derived from lipids and lipid precursors. Mutat Res. 1990;238(3):223\u201333.","journal-title":"Mutat Res"},{"issue":"3","key":"1786_CR28","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1111\/j.1471-4159.2007.05175.x","volume":"105","author":"EK Long","year":"2008","unstructured":"Long EK, Murphy TC, Leiphon LJ, Watt J, Morrow JD, Milne GL, Howard JR, Picklo MJ Sr. Trans-4-hydroxy-2-hexenal is a neurotoxic product of docosahexaenoic (22:6; n-3) acid oxidation. J Neurochem. 2008;105(3):714\u201324.","journal-title":"J Neurochem"},{"key":"1786_CR29","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/s12986-018-0252-4","volume":"15","author":"T Leger","year":"2018","unstructured":"Leger T, Hininger-Favier I, Capel F, Geloen A, Rigaudi\u00e8re JP, Jouve C, Pitois E, Pineau G, Vaysse C, Chardigny JM, Michalski MC, Malpuech-Brug\u00e8re C, Demaison L. Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet. Nutr Metab (Lond). 2018;15:15.","journal-title":"Nutr Metab (Lond)"},{"issue":"1","key":"1786_CR30","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1002\/cphy.c160006","volume":"7","author":"T Kalogeris","year":"2016","unstructured":"Kalogeris T, Baines CP, Krenz M, Korthuis RJ. Ischemia\/Reperfusion. Compr Physiol. 2016;7(1):113\u201370.","journal-title":"Compr Physiol"},{"issue":"10","key":"1786_CR31","doi-asserted-by":"publisher","first-page":"2069","DOI":"10.1194\/jlr.M026179","volume":"53","author":"M Awada","year":"2012","unstructured":"Awada M, Soulage CO, Meynier A, et al. Dietary oxidized n-3 PUFA induce oxidative stress and inflammation: role of intestinal absorption of 4-HHE and reactivity in intestinal cells. J Lipid Res. 2012;53(10):2069\u201380.","journal-title":"J Lipid Res"},{"issue":"13","key":"1786_CR32","doi-asserted-by":"publisher","first-page":"1245","DOI":"10.1056\/NEJMoa1611942","volume":"376","author":"CT Collins","year":"2017","unstructured":"Collins CT, Makrides M, McPhee AJ, Sullivan TR, Davis PG, Thio M, Simmer K, Rajadurai VS, Travadi J, Berry MJ, Liley HG, Opie GF, Tan K, Lui K, Morris SA, Stack J, Stark MJ, Chua MC, Jayagobi PA, Holberton J, Bolisetty S, Callander IR, Harris DL, Gibson RA. Docosahexaenoic acid and bronchopulmonary dysplasia in preterm infants. N Engl J Med. 2017;376(13):1245\u201355.","journal-title":"N Engl J Med"},{"issue":"1","key":"1786_CR33","doi-asserted-by":"publisher","first-page":"20190181","DOI":"10.1542\/peds.2019-0181","volume":"144","author":"Q Wang","year":"2019","unstructured":"Wang Q, Zhou B, Cui Q, Chen C. Omega-3 long-chain polyunsaturated fatty acids for bronchopulmonary dysplasia: a meta-analysis. Pediatrics. 2019;144(1):20190181.","journal-title":"Pediatrics"},{"issue":"4","key":"1786_CR34","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.siny.2010.04.001","volume":"15","author":"JM Davis","year":"2010","unstructured":"Davis JM, Auten RL. Maturation of the antioxidant system and the effects on preterm birth. Semin Fetal Neonatal Med. 2010;15(4):191\u20135.","journal-title":"Semin Fetal Neonatal Med"},{"issue":"14","key":"1786_CR35","doi-asserted-by":"publisher","first-page":"6264","DOI":"10.1073\/pnas.92.14.6264","volume":"92","author":"LM Carlsson","year":"1995","unstructured":"Carlsson LM, Jonsson J, Edlund T, Marklund SL. Mice lacking extracellular superoxide dismutase are more sensitive to hyperoxia. Proc Natl Acad Sci USA. 1995;92(14):6264\u20138.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"7","key":"1786_CR36","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1172\/JCI3816","volume":"103","author":"RJ Folz","year":"1999","unstructured":"Folz RJ, Abushamaa AM, Suliman HB. Extracellular superoxide dismutase in the airways of transgenic mice reduces inflammation and attenuates lung toxicity following hyperoxia. J Clin Invest. 1999;103(7):1055\u201366.","journal-title":"J Clin Invest"},{"issue":"4","key":"1786_CR37","doi-asserted-by":"publisher","first-page":"L777","DOI":"10.1152\/ajplung.00011.2002","volume":"283","author":"TD Oury","year":"2002","unstructured":"Oury TD, Schaefer LM, Fattman CL, Choi A, Weck KE, Watkins SC. Depletion of pulmonary EC-SOD after exposure to hyperoxia. Am J Physiol Lung Cell Mol Physiol. 2002;283(4):L777\u201384.","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"1786_CR38","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1016\/S0022-3476(79)80441-2","volume":"95","author":"WH Northway Jr","year":"1979","unstructured":"Northway WH Jr. Observations on bronchopulmonary dysplasia. J Pediatr. 1979;95:815\u20138.","journal-title":"J Pediatr"}],"container-title":["Respiratory Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12931-021-01786-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12931-021-01786-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12931-021-01786-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T08:13:11Z","timestamp":1625472791000},"score":1,"resource":{"primary":{"URL":"https:\/\/respiratory-research.biomedcentral.com\/articles\/10.1186\/s12931-021-01786-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,5]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["1786"],"URL":"https:\/\/doi.org\/10.1186\/s12931-021-01786-w","relation":{},"ISSN":["1465-993X"],"issn-type":[{"value":"1465-993X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,5]]},"assertion":[{"value":"27 April 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 June 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 July 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experiments were approved by the State University of New York, Downstate Medical Center Institutional Animal Care and Use Committee, Brooklyn, NY (Protocol #17-10517). Animals were cared for according to the guidelines of the United States Department of Agriculture and the Guide for the Care and Use of Laboratory Animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"196"}}