{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T03:18:53Z","timestamp":1780370333629,"version":"3.54.1"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":34,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Respir Res"],"DOI":"10.1186\/s12931-026-03583-9","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T08:46:28Z","timestamp":1772009188000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Label-free optical fingerprints of hyperoxia-induced lung alterations in preterm rabbits"],"prefix":"10.1186","volume":"27","author":[{"given":"Margherita","family":"Marazzini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrica","family":"Scalera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"De Meo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Ricci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gino","family":"Villetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xabier","family":"Murgia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Zoboli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdinando","family":"Gazza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianmarco","family":"Ferri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Cardarelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"3583_CR1","doi-asserted-by":"crossref","unstructured":"Th\u00e9baud B, Goss KN, Laughon M, Whitsett JA, Abman SH, Steinhorn RH et al. Bronchopulmonary dysplasia. Vol. 5, Nature Reviews Disease Primers. Nature Publishing Group; 2019.","DOI":"10.1038\/s41572-019-0127-7"},{"key":"3583_CR2","doi-asserted-by":"crossref","unstructured":"Jobe AH, Bancalari E. NICHD \/ NHLBI \/ ORD workshop summary. Am J Respir Crit Care Med. 2001;163:1723\u201329.","DOI":"10.1164\/ajrccm.163.7.2011060"},{"key":"3583_CR3","doi-asserted-by":"crossref","unstructured":"Schmidt AR, Ramamoorthy C. Bronchopulmonary dysplasia. Paediatric Anaesthesia. Volume 32. John Wiley and Sons Inc; 2022. pp. 174\u201380.","DOI":"10.1111\/pan.14365"},{"key":"3583_CR4","doi-asserted-by":"crossref","unstructured":"Hilgendorff A, O\u2019Reilly MA. Bronchopulmonary dysplasia early changes leading to long-term consequences. 2, Frontiers in Medicine. 2015.","DOI":"10.3389\/fmed.2015.00002"},{"key":"3583_CR5","doi-asserted-by":"crossref","unstructured":"Baraldi E, Carraro S, Filippone M. Bronchopulmonary dysplasia: definitions and long-term respiratory outcome. Early Hum Dev. 2009;85:S1\u2013S3","DOI":"10.1016\/j.earlhumdev.2009.08.002"},{"key":"3583_CR6","doi-asserted-by":"crossref","unstructured":"Richter J, Toelen J, Vanoirbeek J, Kakigano A, DeKoninck P, Verbeken E, et al. Functional assessment of hyperoxia-induced lung injury after preterm birth in the rabbit. Am J Physiol Lung Cell Mol Physiol. 2014;306(3):L277\u201383","DOI":"10.1152\/ajplung.00315.2013"},{"key":"3583_CR7","doi-asserted-by":"crossref","unstructured":"O\u2019Reilly M, Th\u00e9baud B. Animal models of bronchopulmonary dysplasia. The term rat models. Am J Physiol Lung Cell Mol Physiol. 2014;307:L948\u201358.","DOI":"10.1152\/ajplung.00160.2014"},{"key":"3583_CR8","doi-asserted-by":"crossref","unstructured":"Berger J, Bhandari V. Animal models of bronchopulmonary dysplasia. The term mouse models. Am J Physiol Lung Cell Mol Physiol. 2014;307:L936\u201347.","DOI":"10.1152\/ajplung.00159.2014"},{"key":"3583_CR9","doi-asserted-by":"crossref","unstructured":"Salaets T, Gie A, Tack B, Deprest J, Toelen J. Modelling bronchopulmonary dysplasia in animals: arguments for the preterm rabbit model. Curr Pharm Des. 2017;23:5887\u2013901","DOI":"10.2174\/1381612823666170926123550"},{"key":"3583_CR10","doi-asserted-by":"crossref","unstructured":"Manzano RM, Mascaretti RS, Carrer V, Haddad LB, Fernandes AR, Reyes AMA, et al. A hyperoxic lung injury model in premature rabbits: the influence of different gestational ages and oxygen concentrations. PLoS ONE. 2014;9(4):e95844.","DOI":"10.1371\/journal.pone.0095844"},{"key":"3583_CR11","doi-asserted-by":"crossref","unstructured":"Jim\u00e9nez J, Richter J, Nagatomo T, Salaets T, Quarck R, Wagennar A, et al. Progressive vascular functional and structural damage in a bronchopulmonary dysplasia model in preterm rabbits exposed to hyperoxia. Int J Mol Sci. 2016;17(10):1776.","DOI":"10.3390\/ijms17101776"},{"key":"3583_CR12","doi-asserted-by":"crossref","unstructured":"M\u00fchlfeld C, Schulte H, Jansing JC, Casiraghi C, Ricci F, Catozzi C et al. Design-Based Stereology of the Lung in the Hyperoxic Preterm Rabbit Model of Bronchopulmonary Dysplasia. Oxid Med Cell Longev. 2021;2021.","DOI":"10.1155\/2021\/4293279"},{"key":"3583_CR13","doi-asserted-by":"crossref","unstructured":"Storti M, Faietti ML, Murgia X, Catozzi C, Minato I, Tatoni D et al. Time-resolved transcriptomic profiling of the developing rabbit\u2019s lungs: impact of premature birth and implications for modelling bronchopulmonary dysplasia. Respir Res. 2023;24(1).","DOI":"10.1186\/s12931-023-02380-y"},{"issue":"1","key":"3583_CR14","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1186\/s12931-024-03053-0","volume":"26","author":"C Catozzi","year":"2025","unstructured":"Catozzi C, Stretti F, Scalera E, Storti M, Modena A, Aquila G, et al. Single, double, and triple-hit strategies to Establish a long-term premature rabbit model of bronchopulmonary dysplasia. Respir Res. 2025;26(1):35.","journal-title":"Respir Res"},{"key":"3583_CR15","unstructured":"Suvarna SK, Layton C, Bancroft JD. Bancroft\u2019s Theory and Practice of Histological Techniques, Eighth Edition. Bancroft\u2019s Theory and Practice of Histological Techniques, Eighth Edition. 2018."},{"key":"3583_CR16","doi-asserted-by":"crossref","unstructured":"Tadrous PJ. Diagnostic Criteria Handbook in Histopathology: A Surgical Pathology Vade Mecum. Diagnostic Criteria Handbook in Histopathology: A Surgical Pathology Vade Mecum. 2008.","DOI":"10.1002\/9780470723982"},{"key":"3583_CR17","doi-asserted-by":"crossref","unstructured":"Salaets T, Tack B, Jimenez J, Gie A, Lesage F, de Winter D, et al. Simvastatin attenuates lung functional and vascular effects of hyperoxia in preterm rabbits. Pediatr Res. 2020;87(7):1193\u2013200.","DOI":"10.1038\/s41390-019-0711-2"},{"key":"3583_CR18","doi-asserted-by":"crossref","unstructured":"Salaets T, Gie A, Jimenez J, Aertgeerts M, Gheysens O, Velde G, Vande, et al. Local pulmonary drug delivery in the preterm rabbit: feasibility and efficacy of daily intratracheal injections. Am J Physiol Lung Cell Mol Physiol. 2019;316(4):L589\u201397.","DOI":"10.1152\/ajplung.00255.2018"},{"key":"3583_CR19","doi-asserted-by":"crossref","unstructured":"Aquila G, Regin Y, Murgia X, Salomone F, Casiraghi C, Catozzi C, et al. Daily intraperitoneal administration of Rosiglitazone does not improve lung function or alveolarization in preterm rabbits exposed to hyperoxia. Pharmaceutics. 2022;14(7):1507.","DOI":"10.3390\/pharmaceutics14071507"},{"key":"3583_CR20","doi-asserted-by":"crossref","unstructured":"Thurlbeck WM. The radial alveolar count method of Emery and mithal: a reappraisal 2-Intrauterine and early postnatal lung growth THOMAS P Cooney. Thorax. 1982;37(8):580\u20133.","DOI":"10.1136\/thx.37.8.580"},{"key":"3583_CR21","doi-asserted-by":"crossref","unstructured":"Emery JL, Mithal A. The number of alveoli in the terminal respiratory unit of man during late intrauterine life and childhood. Arch Dis Child. 1960;35(184):544\u20137.","DOI":"10.1136\/adc.35.184.544"},{"key":"3583_CR22","unstructured":"Roubliova XI, Deprest JA, Biard JM, Ophalvens L, Gallot D, Jani JC, et al. Morphologic changes and methodological issues in the rabbit experimental model for diaphragmatic hernia. Histol Histopathol. 2010;25(9):1105\u201316."},{"key":"3583_CR23","doi-asserted-by":"crossref","unstructured":"Matute-Bello G, Downey G, Moore BB, Groshong SD, Matthay MA, Slutsky AS et al. An official American thoracic society workshop report: features and measurements of experimental acute lung injury in animals. In: Am J Respir Cell Mol Biol. 2011. pp. 725\u201338.","DOI":"10.1165\/rcmb.2009-0210ST"},{"key":"3583_CR24","doi-asserted-by":"crossref","unstructured":"Dolber PC, Spach MS. Conventional and confocal fluorescence microscopy of collagen fibers in the heart. J Histochem Cytochem. 1993;41(3):465\u20139.","DOI":"10.1177\/41.3.7679127"},{"key":"3583_CR25","doi-asserted-by":"crossref","unstructured":"Wegner KA, Keikhosravi A, Eliceiri KW, Vezina CM. Fluorescence of Picrosirius red multiplexed with immunohistochemistry for the quantitative assessment of collagen in tissue sections. J Histochem Cytochem. 2017;65(8):479\u201390.","DOI":"10.1369\/0022155417718541"},{"key":"3583_CR26","doi-asserted-by":"crossref","unstructured":"Mosta\u00e7o-Guidolin L, Rosin NL, Hackett TL. Imaging collagen in Scar tissue: developments in second harmonic generation microscopy for biomedical applications. Int J Mol Sci. 2017;18:1772.","DOI":"10.3390\/ijms18081772"},{"key":"3583_CR27","doi-asserted-by":"crossref","unstructured":"Chance B, Schoener B, Oshino R, Itshak F, Nakase Y. Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples. NADH and Flavoprotein fluorescence signals. J Biol Chem. 1979;254(11):4764\u201371.","DOI":"10.1016\/S0021-9258(17)30079-0"},{"key":"3583_CR28","doi-asserted-by":"crossref","unstructured":"Azzarello F, Pesce L, De Lorenzi V, Ferri G, Tesi M, Del Guerra S, et al. Single-cell imaging of \u03b1 and \u03b2 cell metabolic response to glucose in living human Langerhans Islets. Commun Biol. 2022;5(1):1232.","DOI":"10.1038\/s42003-022-04215-w"},{"key":"3583_CR29","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1073\/pnas.1108161108","volume":"108","author":"C Stringari","year":"2011","unstructured":"Stringari C, Cinquin A, Cinquin O, Digman MA, Donovan PJ, Gratton E. Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic States of germ cells in a live tissue. Proc Natl Acad Sci U S A. 2011;108:33.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3583_CR30","doi-asserted-by":"publisher","first-page":"07","DOI":"10.1117\/1.JBO.25.7.071203","volume":"25","author":"R Datta","year":"2020","unstructured":"Datta R, Heaster TM, Sharick JT, Gillette AA, Skala MC. Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications. J Biomed Opt. 2020;25:07.","journal-title":"J Biomed Opt"},{"key":"3583_CR31","doi-asserted-by":"crossref","unstructured":"Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT, Webb WW. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Vol. 100, PNAS. 2003. Available from: https:\/\/www.pnas.org\/doi\/10.1073\/pnas.0832308100","DOI":"10.1073\/pnas.0832308100"},{"key":"3583_CR32","doi-asserted-by":"crossref","unstructured":"Digman MA, Caiolfa VR, Zamai M, Gratton E. The phasor approach to fluorescence lifetime imaging analysis. Biophys J. 2008;94(2):L14\u20136.","DOI":"10.1529\/biophysj.107.120154"},{"key":"3583_CR33","doi-asserted-by":"crossref","unstructured":"Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9:676\u201382.","DOI":"10.1038\/nmeth.2019"},{"key":"3583_CR34","doi-asserted-by":"crossref","unstructured":"Huang LK, Wang MJJ. Image thresholding by minimizing the measures of fuzziness. Pattern Recognit. 1995;28(1):41\u201351.","DOI":"10.1016\/0031-3203(94)E0043-K"},{"key":"3583_CR35","doi-asserted-by":"crossref","unstructured":"Crowley G, Kwon S, Caraher EJ, Haider SH, Lam R, Batra P, et al. Quantitative lung morphology: semi-automated measurement of mean linear intercept. BMC Pulm Med. 2019;19(1):206.","DOI":"10.1186\/s12890-019-0915-6"},{"key":"3583_CR36","doi-asserted-by":"crossref","unstructured":"Frangi AF, Niessen WJ, Vincken KL, Viergever MA. Multiscale vessel enhancement filtering. In: Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics). 1998.","DOI":"10.1007\/BFb0056195"},{"key":"3583_CR37","doi-asserted-by":"crossref","unstructured":"Nejim Z, Navarro L, Morin C, Badel P. Quantitative analysis of second harmonic generated images of collagen fibers: a review. Research on Biomedical Engineering. Volume 39. Springer Science and Business Media Deutschland GmbH; 2023. pp. 273\u201395.","DOI":"10.1007\/s42600-022-00250-y"},{"issue":"1","key":"3583_CR38","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1109\/TSMC.1979.4310076","volume":"9","author":"N Otsu","year":"1979","unstructured":"Otsu N. A threshold selection method from Gray-Level histograms. IEEE Trans Syst Man Cybern. 1979;9(1):62\u20136.","journal-title":"IEEE Trans Syst Man Cybern"},{"key":"3583_CR39","doi-asserted-by":"crossref","unstructured":"Roth S, Freund I. Optical second-harmonic scattering in rat\u2010tail tendon. Biopolymers. 1981;20(6):1271\u201390.","DOI":"10.1002\/bip.1981.360200613"},{"key":"3583_CR40","doi-asserted-by":"crossref","unstructured":"Junqueira LCU, Bignolas G, Brentani RR. Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem J. 1979;11(4):447\u201355.","DOI":"10.1007\/BF01002772"},{"key":"3583_CR41","doi-asserted-by":"crossref","unstructured":"Pisano ED, Zong S, Hemminger BM, Deluca M, Johnston RE, Muller K, et al. Contrast limited adaptive histogram equalization image processing to improve the detection of simulated spiculations in dense mammograms. J Digit Imaging. 1998;11:193\u2013200.","DOI":"10.1007\/BF03178082"},{"key":"3583_CR42","doi-asserted-by":"crossref","unstructured":"Huang TS, Yang GJ, Tang GY. A fast two-dimensional median filtering algorithm. IEEE Trans Acoust. 1979;27(1):13\u201318.","DOI":"10.1109\/TASSP.1979.1163188"},{"key":"3583_CR43","doi-asserted-by":"crossref","unstructured":"Berezin MY, Achilefu S. Fluorescence lifetime measurements and biological imaging. Chem Rev. 2010;110(5):2641\u201384.","DOI":"10.1021\/cr900343z"},{"key":"3583_CR44","doi-asserted-by":"crossref","unstructured":"Mereness JA, Mariani TJ. The critical role of collagen VI in lung development and chronic lung disease. Volume 10. Matrix Biology Plus; 2021.","DOI":"10.1016\/j.mbplus.2021.100058"},{"key":"3583_CR45","doi-asserted-by":"crossref","unstructured":"Mi\u017e\u00edkov\u00e1 I, Morty RE. The extracellular matrix in bronchopulmonary dysplasia: Target and source. Vol. 2, Frontiers in Medicine. 2015.","DOI":"10.3389\/fmed.2015.00091"},{"key":"3583_CR46","doi-asserted-by":"crossref","unstructured":"Kalikkot Thekkeveedu R, Guaman MC, Shivanna B. Bronchopulmonary dysplasia: A review of pathogenesis and pathophysiology. Vol. 132, Respiratory Medicine. 2017.","DOI":"10.1016\/j.rmed.2017.10.014"},{"key":"3583_CR47","doi-asserted-by":"crossref","unstructured":"Coalson JJ. Pathology of new bronchopulmonary dysplasia. Vol. 8, Seminars in Neonatology. 2003.","DOI":"10.1016\/S1084-2756(02)00193-8"},{"key":"3583_CR48","doi-asserted-by":"crossref","unstructured":"Mi\u017e\u00edkov\u00e1 I, Ruiz-Camp J, Steenbock H, Madurga A, Vad\u00e1sz I, Herold S, et al. Collagen and Elastin cross-linking is altered during aberrant late lung development associated with hyperoxia. Am J Physiol Lung Cell Mol Physiol. 2015;308(11):L1145\u201358.","DOI":"10.1152\/ajplung.00039.2015"},{"key":"3583_CR49","doi-asserted-by":"crossref","unstructured":"Kumarasamy A, Schmitt I, Nave AH, Reiss I, Van Der Horst I, Dony E, et al. Lysyl oxidase activity is dysregulated during impaired alveolarization of mouse and human lungs. Am J Respir Crit Care Med. 2009;180(12):1239\u201352.","DOI":"10.1164\/rccm.200902-0215OC"},{"key":"3583_CR50","doi-asserted-by":"crossref","unstructured":"Gie AG, Regin Y, Salaets T, Casiraghi C, Salomone F, Deprest J, et al. Intratracheal budesonide\/surfactant attenuates hyperoxia-induced lung injury in preterm rabbits. Am J Physiol Lung Cell Mol Physiol. 2020;319(6):L949\u201356.","DOI":"10.1152\/ajplung.00162.2020"},{"key":"3583_CR51","doi-asserted-by":"crossref","unstructured":"Schittny JC. Development of the lung. Cell and Tissue Research. Volume 367. Springer; 2017. pp. 427\u201344.","DOI":"10.1007\/s00441-016-2545-0"},{"key":"3583_CR52","doi-asserted-by":"crossref","unstructured":"Lignelli E, Palumbo F, Myti D, Morty RE. Recent advances in our understanding of the mechanisms of lung alveolarization and bronchopulmonary dysplasia. Am J Physiol Lung Cell Mol Physiol. 2019;317:L832\u201387.","DOI":"10.1152\/ajplung.00369.2019"},{"key":"3583_CR53","doi-asserted-by":"crossref","unstructured":"Bhatt AJ, Pryhuber GS, Huyck H, Watkins RH, Metlay LA, Maniscalco WM. Disrupted pulmonary vasculature and decreased vascular endothelial growth factor, Flt-1, and TIE-2 in human infants dying with bronchopulmonary dysplasia. Am J Respir Crit Care Med. 2001;164(10 I):1971\u201380.","DOI":"10.1164\/ajrccm.164.10.2101140"},{"key":"3583_CR54","doi-asserted-by":"crossref","unstructured":"De Paepe ME, Mao Q, Powell J, Rubin SE, DeKoninck P, Appel N, et al. Growth of pulmonary microvasculature in ventilated preterm infants. Am J Respir Crit Care Med. 2006;173(2):204\u201311.","DOI":"10.1164\/rccm.200506-927OC"},{"key":"3583_CR55","doi-asserted-by":"crossref","unstructured":"Alvira CM. Aberrant pulmonary vascular growth and remodeling in bronchopulmonary dysplasia. Front Med. 2016;3:21.","DOI":"10.3389\/fmed.2016.00021"},{"issue":"1","key":"3583_CR56","doi-asserted-by":"publisher","first-page":"14235","DOI":"10.1038\/s41598-024-65161-7","volume":"14","author":"F Azzarello","year":"2024","unstructured":"Azzarello F, Carli F, De Lorenzi V, Tesi M, Marchetti P, Beltram F, et al. Machine-learning-guided recognition of \u03b1 and \u03b2 cells from label-free infrared micrographs of living human Islets of Langerhans. Sci Rep. 2024;14(1):14235.","journal-title":"Sci Rep"},{"key":"3583_CR57","doi-asserted-by":"crossref","unstructured":"Ranjit S, Malacrida L, Jameson DM, Gratton E. Fit-free analysis of fluorescence lifetime imaging data using the phasor approach. Nat Protoc. 2018;13(9):1979\u20132004.","DOI":"10.1038\/s41596-018-0026-5"},{"key":"3583_CR58","doi-asserted-by":"crossref","unstructured":"Datta R, Alfonso-Garci\u00e1 A, Cinco R, Gratton E. Fluorescence lifetime imaging of endogenous biomarker of oxidative stress. Sci Rep. 2015;5:9848.","DOI":"10.1038\/srep09848"},{"key":"3583_CR59","doi-asserted-by":"crossref","unstructured":"da Silva FAB, Racanelli AP, Lorand-Metze I, Metze K. Fluorescence lifetime imaging is able to recognize different hematopoietic precursors in unstained routine bone marrow films. Cytometry Part A. 2021;99(6):641\u20136.","DOI":"10.1002\/cyto.a.24345"}],"container-title":["Respiratory Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12931-026-03583-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12931-026-03583-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12931-026-03583-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T14:28:59Z","timestamp":1774967339000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12931-026-03583-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,25]]},"references-count":59,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["3583"],"URL":"https:\/\/doi.org\/10.1186\/s12931-026-03583-9","relation":{},"ISSN":["1465-993X"],"issn-type":[{"value":"1465-993X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,25]]},"assertion":[{"value":"28 July 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experimental procedures involving animals were approved by the local animal ethics committee and met the standard European regulations on animal research (n\u00b0783\/2019-PR).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"ES, FR, GV are employees of Chiesi Farmaceutici S.p.A. XM served as a consultant for this study. The other authors have no conflicts of interest to declare.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"153"}}