{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:57:46Z","timestamp":1792173466165,"version":"4.4.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"name":"Zhejiang Provincial Natural Acience Foundation of China","award":["LY16H200003"],"award-info":[{"award-number":["LY16H200003"]}]},{"name":"Zhejiang Provincial Health and Family Planning Commission of China","award":["2017KY218"],"award-info":[{"award-number":["2017KY218"]}]},{"DOI":"10.13039\/501100005230","name":"Chongqing Natural Science Foundation","doi-asserted-by":"crossref","award":["cstc2015jcyjA10003"],"award-info":[{"award-number":["cstc2015jcyjA10003"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Chongqing Health and Family Planning Commission of China","award":["2013-2-216"],"award-info":[{"award-number":["2013-2-216"]}]},{"DOI":"10.13039\/501100001809","name":"Chinese National Natural Science Foundation","doi-asserted-by":"crossref","award":["81661148057"],"award-info":[{"award-number":["81661148057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s12934-017-0801-y","type":"journal-article","created":{"date-parts":[[2017,11,14]],"date-time":"2017-11-14T11:29:40Z","timestamp":1510658980000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Structure-based antigenic epitope and PEGylation improve the efficacy of staphylokinase"],"prefix":"10.1186","volume":"16","author":[{"given":"Yanying","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyuan","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiangsi","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deqiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,11,14]]},"reference":[{"issue":"6","key":"801_CR1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.amjmed.2015.11.033","volume":"129","author":"V Gurewich","year":"2016","unstructured":"Gurewich V. Thrombolysis: a critical first-line\u00a0therapy\u00a0with an unfulfilled potential. Am J Med. 2016;129(6):573\u20135.","journal-title":"Am J Med"},{"issue":"Suppl 3","key":"801_CR2","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.thromres.2008.06.018","volume":"122","author":"V Novokhatny","year":"2008","unstructured":"Novokhatny V. Structure and activity of plasmin and other direct thrombolytic agents. Thromb Res. 2008;122(Suppl 3):S3\u20138.","journal-title":"Thromb Res"},{"issue":"1","key":"801_CR3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11684-017-0497-8","volume":"11","author":"ME Klegerman","year":"2017","unstructured":"Klegerman ME. Translational initiatives in thrombolytic therapy. Front Med. 2017;11(1):1\u201319.","journal-title":"Front Med"},{"key":"801_CR4","first-page":"586510","volume":"2014","author":"MR Ali","year":"2014","unstructured":"Ali MR, Salim Hossain M, Islam MA, Saiful Islam Arman M, et al. Aspect of thrombolytic therapy: a review. Sci World J. 2014;2014:586510.","journal-title":"Sci World J"},{"issue":"7","key":"801_CR5","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1111\/j.1747-4949.2012.00923.x","volume":"8","author":"K Butcher","year":"2013","unstructured":"Butcher K, Shuaib A, Saver J, et al. Thrombolysis in the developing world: is there a role for\u00a0streptokinase? Int J Stroke. 2013;8(7):560\u20135.","journal-title":"Int J Stroke."},{"issue":"2","key":"801_CR6","doi-asserted-by":"crossref","first-page":"106","DOI":"10.2174\/1574890110999150202150017","volume":"9","author":"A Kunamneni","year":"2014","unstructured":"Kunamneni A, Durvasula R. Streptokinase-A drug for thrombolytic therapy: a patent review. Recent Adv Cardiovasc Drug Discov. 2014;9(2):106\u201321.","journal-title":"Recent Adv Cardiovasc Drug Discov."},{"issue":"11","key":"801_CR7","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.5858\/2002-126-1376-PATTPA","volume":"126","author":"JT Brandt","year":"2002","unstructured":"Brandt JT. Plasminogen and tissue-type plasminogen activator deficiency as risk factors for thromboembolic disease. Arch Pathol Lab Med. 2002;126(11):1376\u201381.","journal-title":"Arch Pathol Lab Med"},{"key":"801_CR8","doi-asserted-by":"crossref","first-page":"8284","DOI":"10.1016\/S0021-9258(18)53094-5","volume":"268","author":"D Collen","year":"1993","unstructured":"Collen D, Schlott B, Engelborghs Y, et al. On the mechanism of the activation of human plasminogen by recombinant staphylokinase. J Biol Chem. 1993;268:8284\u20139.","journal-title":"J Biol Chem"},{"key":"801_CR9","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1432-1033.1993.tb18147.x","volume":"216","author":"D Collen","year":"1993","unstructured":"Collen D, VanHoef B, Schlott B, et al. Mechanisms of activation of mamma-lian plasma \u00aebrinolytic systems with streptokinase and with recombinant staphylokinase. Eur J Biochem. 1993;216:307\u201314.","journal-title":"Eur J Biochem"},{"key":"801_CR10","doi-asserted-by":"crossref","first-page":"31817","DOI":"10.1038\/srep31817","volume":"6","author":"LT Nguyen","year":"2016","unstructured":"Nguyen LT, Vogel HJ. Staphylokinase is its ability to neutralize host antimicrobial peptides whose binding may control its plasminogen activation properties. Sci Rep. 2016;6:31817.","journal-title":"Sci Rep"},{"key":"801_CR11","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nm0398-279","volume":"4","author":"D Collen","year":"1998","unstructured":"Collen D. Staphylokinase: a potent, uniquely fibrin selective thrombolytic agent. Nature Med. 1998;4:279\u201384.","journal-title":"Nature Med"},{"key":"801_CR12","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1055\/s-0038-1648856","volume":"72","author":"SM Vanderschueren","year":"1994","unstructured":"Vanderschueren SM, Stassen JM, Collen D. On the immunogenicity of recombinant staphylokinase in patients and in animal models. Thromb Haemost. 1994;72:297\u2013301.","journal-title":"Thromb Haemost"},{"key":"801_CR13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1055\/s-0038-1642396","volume":"71","author":"PJ Declerck","year":"1994","unstructured":"Declerck PJ, Vanderschueren S, Billiet J, et al. Prevalence and induction of circulating anti-bodies against recombinant staphylokinase. Thromb Haemost. 1994;71:129\u201333.","journal-title":"Thromb Haemost"},{"issue":"5","key":"801_CR14","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2165\/00063030-200822050-00004","volume":"22","author":"FM Veronese","year":"2008","unstructured":"Veronese FM, Mero A. The impact of PEGylation on biological therapies. BioDrugs. 2008;22(5):315\u201329.","journal-title":"BioDrugs."},{"issue":"1","key":"801_CR15","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/biot.200900218","volume":"5","author":"S Jevsevar","year":"2010","unstructured":"Jevsevar S, Kunstelj M, Porekar VG. PEGylation\u00a0of therapeutic proteins. Biotechnol J. 2010;5(1):113\u201328.","journal-title":"Biotechnol J"},{"key":"801_CR16","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/biot.200900218","volume":"5","author":"S Jevsevar","year":"2010","unstructured":"Jevsevar S, Kunstelj M, Porekar VG. PEGylation of therapeutic proteins. Biotech J. 2010;5:113\u201328.","journal-title":"Biotech J"},{"key":"801_CR17","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/S0169-409X(03)00108-X","volume":"55","author":"P Caliceti","year":"2003","unstructured":"Caliceti P, Veronese FM. Pharmacokinetic and biodistribution properties of poly(ethylene glycol)-protein conjugates. Adv Drug Deliv Rev. 2003;55:1261\u201377.","journal-title":"Adv Drug Deliv Rev"},{"key":"801_CR18","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.cbpa.2016.08.006","volume":"34","author":"PB Lawrence","year":"2016","unstructured":"Lawrence PB, Price JL. How PEGylation influences protein conformational stability. Curr Opin Chem Biol. 2016;34:88\u201394.","journal-title":"Curr Opin Chem Biol"},{"issue":"2","key":"801_CR19","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1021\/bm301511w","volume":"14","author":"X Xue","year":"2013","unstructured":"Xue X, Li D, Yu J, et al. Phenyl linker-induced dense PEG conformation improves the efficacy of C-terminally monoPEGylated staphylokinase. Biomacromolecules. 2013;14(2):331\u201341.","journal-title":"Biomacromolecules"},{"issue":"7","key":"801_CR20","doi-asserted-by":"crossref","first-page":"e68559","DOI":"10.1371\/journal.pone.0068559","volume":"8","author":"Q Mu","year":"2013","unstructured":"Mu Q, Hu T, Yu J. Molecular\u00a0insight\u00a0into the\u00a0steric\u00a0shielding\u00a0effect\u00a0of\u00a0PEG\u00a0on the\u00a0conjugatedstaphylokinase:\u00a0biochemical\u00a0characterization\u00a0and\u00a0molecular\u00a0dynamics\u00a0simulation. PLoS ONE. 2013;8(7):e68559.","journal-title":"PLoS ONE"},{"key":"801_CR21","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.procbio.2011.10.004","volume":"47","author":"R Liu","year":"2012","unstructured":"Liu R, Li D, Wang J, et al. Preparation, characterization and in vitro bioactivity of Nterminally PEGylated staphylokinase dimers. Process Biochem. 2012;47:41\u20136.","journal-title":"Process Biochem"},{"issue":"3","key":"801_CR22","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1182\/blood.V95.3.936.003k20_936_942","volume":"95","author":"S Vanwetswinkel","year":"2000","unstructured":"Vanwetswinkel S, Plaisance S, Zhi-Yong Z, et al. Pharmacokinetic and thrombolytic properties of cysteine-linked polyethylene glycol derivatives of staphylokinase. Blood. 2000;95(3):936\u201342.","journal-title":"Blood"},{"key":"801_CR23","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nsb0597-357","volume":"4","author":"A Rabijns","year":"1997","unstructured":"Rabijns A, De Bondt HL, De Ranter C. Three-dimensional structure of staphylokinase, a plasminogen activator with therapeutic potential. Nat Struct Biol. 1997;4:357\u201360.","journal-title":"Nat Struct Biol"},{"key":"801_CR24","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/2359","volume":"5","author":"MA Parry","year":"1998","unstructured":"Parry MA, Fernandez-Catalan C, Bergner A, Huber R, et al. The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action. Nat Struct Biol. 1998;5:917\u201323.","journal-title":"Nat Struct Biol"},{"issue":"1","key":"801_CR25","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/bit.21181","volume":"96","author":"A Slavica","year":"2007","unstructured":"Slavica A, Dib I, Nidetzky B. Selective modification of surface-exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using\u00a0poly(ethylene\u00a0glycol) maleimide and its effect on activityand\u00a0stability of the\u00a0enzyme. Biotechnol Bioeng. 2007;96(1):9\u201317.","journal-title":"Biotechnol Bioeng"},{"issue":"9","key":"801_CR26","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1093\/abbs\/gmu068","volume":"46","author":"J Liu","year":"2014","unstructured":"Liu J, Wang Z, He J, et al. Effect of site-specific PEGylaion on the fibrinolytic activity,\u00a0immunogenicity, and pharmacokinetics of staphylokinase. Acta Biochim Biophys Sin (Shanghai). 2014;46(9):782\u201391.","journal-title":"Acta Biochim Biophys Sin (Shanghai)"},{"key":"801_CR27","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1084\/jem.148.1.223","volume":"148","author":"A Granelli-Piperno","year":"1978","unstructured":"Granelli-Piperno A, Reich E. A study of proteases and proteaseinhibitor complexes in biological fluids. J Exp Med. 1978;148:223\u201334.","journal-title":"J Exp Med"},{"key":"801_CR28","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S0167-4838(01)00161-3","volume":"1546","author":"J Jiao","year":"2001","unstructured":"Jiao J, Yu M, Ru B. Characterization of a recombinant chimeric plasminogen activator with enhanced fibrin binding. Biochim Biophys Acta. 2001;1546:399\u2013405.","journal-title":"Biochim Biophys Acta"},{"key":"801_CR29","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.jbiotec.2006.04.037","volume":"1126","author":"FX Ding","year":"2006","unstructured":"Ding FX, Yan HL, Lu YM, et al. Cloning, purification and biochemical characterization of a thrombus-ditargeting thrombolytic agent, comprised of annexin B1, ScuPA-32\u00a0K and fibrin-adherent peptide. J Biotechnol. 2006;1126:394\u2013405.","journal-title":"J Biotechnol"},{"key":"801_CR30","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s12896-015-0127-y","volume":"15","author":"X Long","year":"2015","unstructured":"Long X, Gou Y, Luo M, et al. Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coli. BMC Biotechnol. 2015;15:13.","journal-title":"BMC Biotechnol"},{"issue":"8","key":"801_CR31","doi-asserted-by":"crossref","first-page":"e0136463","DOI":"10.1371\/journal.pone.0136463","volume":"10","author":"M Panigada","year":"2015","unstructured":"Panigada M, Zacchetti L, L\u2019Acqua C, Cressoni M, et al. Assessment of fibrinolysis in sepsis patients with urokinase modified thromboelastography. PLoS ONE. 2015;10(8):e0136463.","journal-title":"PLoS ONE"},{"issue":"5","key":"801_CR32","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/S1734-1140(11)70636-3","volume":"63","author":"J Szemraj","year":"2011","unstructured":"Szemraj J, Zakrzeska A, Brown G, et al. New derivative of staphylokinase Sak-RGD-K2-Hirul exerts thrombolytic effects in the arterial thrombosis model in\u00a0rats. Pharmacol Rep. 2011;63(5):1169\u201379.","journal-title":"Pharmacol Rep."},{"issue":"2","key":"801_CR33","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.xphs.2015.11.015","volume":"105","author":"PL Turecek","year":"2016","unstructured":"Turecek PL, Bossard MJ, Schoetens F, Ivens IA. PEGylation\u00a0of biopharmaceuticals: a\u00a0review\u00a0of chemistry and nonclinical safety information of approved drugs. J Pharm Sci. 2016;105(2):460\u201375.","journal-title":"J Pharm Sci"},{"issue":"6","key":"801_CR34","doi-asserted-by":"crossref","first-page":"3161","DOI":"10.4049\/jimmunol.161.6.3161","volume":"161","author":"S Jenn\u00e9","year":"1998","unstructured":"Jenn\u00e9 S, Brepoels K, Collen D, Jespers L. High resolution mapping of the B cell epitopes of staphylokinase in humans using negative selection of a phage-displayed antigen library. J Immunol. 1998;161(6):3161\u20138.","journal-title":"J Immunol."}],"container-title":["Microbial Cell Factories"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-017-0801-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T13:21:35Z","timestamp":1693228895000},"score":1,"resource":{"primary":{"URL":"https:\/\/microbialcellfactories.biomedcentral.com\/articles\/10.1186\/s12934-017-0801-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,14]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["801"],"URL":"https:\/\/doi.org\/10.1186\/s12934-017-0801-y","relation":{},"ISSN":["1475-2859"],"issn-type":[{"value":"1475-2859","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,14]]},"article-number":"197"}}