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However, recombinant production of malaria proteins is notoriously difficult. To address this, we have investigated two putative K<jats:sup>+<\/jats:sup> channels, PfKch1 and PfKch2, identified in the <jats:italic>P. falciparum<\/jats:italic> genome. We show that PfKch1 and PfKch2 and a C-terminally truncated version of PfKch1 (PfKch1<jats:sup>1\u22121094<\/jats:sup>) could indeed be functionally expressed in vivo, since a K<jats:sup>+<\/jats:sup>-uptake deficient <jats:italic>Saccharomyces cerevisiae<\/jats:italic> strain was complemented by the <jats:italic>P. falciparum<\/jats:italic> cDNAs. PfKch1<jats:sup>1\u22121094<\/jats:sup>-GFP and GFP-PfKch2 fusion proteins were overexpressed in yeast, purified and reconstituted in lipid bilayers to determine their electrophysiological activity. Single channel conductance amounted to 16\u2009\u00b1\u20091 pS for PfKch1<jats:sup>1\u22121094<\/jats:sup>-GFP and 28\u2009\u00b1\u20092 pS for GFP-PfKch2. We predicted regulator of\u00a0K<jats:sup>+<\/jats:sup>-conductance (RCK) domains in the C-terminals of both channels, and we accordingly measured channel activity in the presence of Ca<jats:sup>2+.<\/jats:sup><\/jats:p>","DOI":"10.1186\/s12934-020-01437-7","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T13:03:52Z","timestamp":1600693432000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Purification and initial characterization of Plasmodium falciparum K+ channels, PfKch1 and PfKch2 produced in Saccharomyces cerevisiae"],"prefix":"10.1186","volume":"19","author":[{"given":"Karen","family":"Molbaek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Tejada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina Hoeier","family":"Ricke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Scharff-Poulsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Ellekvist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Helix-Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirbhay","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan A.","family":"Klaerke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8648-9748","authenticated-orcid":false,"given":"Per Amstrup","family":"Pedersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,9,21]]},"reference":[{"key":"1437_CR1","doi-asserted-by":"publisher","unstructured":"Voices, l. e. 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