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Complementary chromatic acclimation (CCA), a photoreversible process that facilitates the control of cellular expression using light of different wavelengths in cyanobacteria, is one example. In this study, an artificial CCA systems, inspired by type III CCA light-regulated gene expression, was designed by employing a single photosensor system, the CcaS\/CcaR green light gene expression system derived from <jats:italic>Synechocystis<\/jats:italic> sp. PCC6803, combined with G-box (the regulator recognized by activated CcaR), the cognate <jats:italic>cpcG2<\/jats:italic> promoter, and the constitutively transcribed promoter, the P<jats:sub><jats:italic>trc\u0394LacO<\/jats:italic><\/jats:sub> promoter.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>One G-box was inserted upstream of the <jats:italic>cpcG2<\/jats:italic> promoter and a reporter gene, the <jats:italic>rfp<\/jats:italic> gene (green light-induced gene expression), and the other G-box was inserted between the P<jats:sub><jats:italic>trc\u0394LacO<\/jats:italic><\/jats:sub> promoter and a reporter gene, the <jats:italic>bfp<\/jats:italic> gene (red light-induced gene expression). The <jats:italic>Escherichia coli<\/jats:italic> transformants with plasmid-encoded genes were evaluated at the transcriptional and translational levels under red or green light illumination. Under green light illumination, the transcription and translation of the <jats:italic>rfp<\/jats:italic> gene were observed, whereas the expression of the <jats:italic>bfp<\/jats:italic> gene was repressed. Under red light illumination, the transcription and translation of the <jats:italic>bfp<\/jats:italic> gene were observed, whereas the expression of the <jats:italic>rfp<\/jats:italic> gene was repressed. During the red and green light exposure cycles at every 6\u00a0h, BFP expression increased under red light exposure while RFP expression was repressed, and RFP expression increased under green light exposure while BFP expression was repressed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS\/CcaR system derived from <jats:italic>Synechocystis<\/jats:italic> sp. PCC6803, in <jats:italic>E. coli<\/jats:italic>. The artificial CCA system functioned repeatedly during red and green light exposure cycles. These results demonstrate the potential application of this CCA gene expression system for the production of multiple metabolites in a variety of microorganisms, such as cyanobacteria.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12934-021-01621-3","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T10:04:33Z","timestamp":1625479473000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Artificial complementary chromatic acclimation gene expression system in Escherichia coli"],"prefix":"10.1186","volume":"20","author":[{"given":"Dwi","family":"Ariyanti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Ikebukuro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9833-2091","authenticated-orcid":false,"given":"Koji","family":"Sode","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,5]]},"reference":[{"key":"1621_CR1","doi-asserted-by":"publisher","first-page":"2214","DOI":"10.1093\/pcp\/pcr155","volume":"52","author":"R Narikawa","year":"2011","unstructured":"Narikawa R, Suzuki F, Yoshihara S, Higashi S, Watanabe M, Ikeuchi M. 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