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The\n                      <jats:italic>acuC<\/jats:italic>\n                      gene is related to the acetylation\/deacetylation posttranslational modification (PTM) system in\n                      <jats:italic>S. spinosa.<\/jats:italic>\n                      Spinosyns, the secondary metabolites produced by\n                      <jats:italic>Saccharopolyspora spinosa<\/jats:italic>\n                      , are the active ingredients in a family of insect control agents. However, the specific functions and influences of acuC protein in\n                      <jats:italic>S. spinosa<\/jats:italic>\n                      are yet to be characterized.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The knockout strain and overexpression strain were constructed separately with the shuttle vector pOJ260. The production of spinosyns A and D from\n                      <jats:italic>S. spinosa-acuC<\/jats:italic>\n                      were 105.02\u00a0mg\/L and 20.63\u00a0mg\/L, which were 1.82-fold and 1.63-fold higher than those of the wild-type strain (57.76\u00a0mg\/L and 12.64\u00a0mg\/L), respectively. The production of spinosyns A and D from\n                      <jats:italic>S. spinosa-<\/jats:italic>\n                      \u0394\n                      <jats:italic>acuC<\/jats:italic>\n                      were 32.78\u00a0mg\/L and 10.89\u00a0mg\/L, respectively. The qRT-PCR results of three selected genes (\n                      <jats:italic>bldD<\/jats:italic>\n                      ,\n                      <jats:italic>ssgA<\/jats:italic>\n                      and\n                      <jats:italic>whiA<\/jats:italic>\n                      ) confirmed that the overexpression of\n                      <jats:italic>acuC<\/jats:italic>\n                      affected the capacities of mycelial differentiation and sporulation. Comparative proteomics analysis was performed on these strains to investigate the underlying mechanism leading to the enhancement of spinosad yield.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study first systematically analysed the effects of overexpression\n                      <jats:italic>acuC<\/jats:italic>\n                      on the growth of\n                      <jats:italic>S. spinosa<\/jats:italic>\n                      and the production of spinosad. The results identify the differentially expressed proteins and provide evidences to understand the acetylation metabolic mechanisms which can lead to the increase of secondary metabolites.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12934-021-01630-2","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T07:03:04Z","timestamp":1626937384000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa"],"prefix":"10.1186","volume":"20","author":[{"given":"Zhudong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianli","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Shuai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyuan","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuezhi","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Rang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8443-2916","authenticated-orcid":false,"given":"Liqiu","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,22]]},"reference":[{"issue":"1","key":"1630_CR1","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1099\/00207713-40-1-34","volume":"40","author":"FP Mertz","year":"1990","unstructured":"Mertz FP, Yao RC. 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