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Especially the optically pure (2R,3R)-2,3-butanediol can be employed as an antifreeze agent and as the precursor for producing chiral compounds. However, some (2R,3R)-2,3-butanediol overproducing strains are pathogenic such as <jats:italic>Enterobacter cloacae<\/jats:italic> and <jats:italic>Klebsiella oxytoca<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In this study, a (3R)-acetoin overproducing <jats:italic>C. glutamicum<\/jats:italic> strain, CGS9, was engineered to produce optically pure (2R,3R)-2,3-butanediol efficiently. Firstly, the gene <jats:italic>bdhA<\/jats:italic> from <jats:italic>B. subtilis<\/jats:italic> 168 was integrated into strain CGS9 and its expression level was further enhanced by using a strong promoter <jats:italic>P<\/jats:italic><jats:sub><jats:italic>sod<\/jats:italic><\/jats:sub> and ribosome binding site (RBS) with high translation initiation rate, and the (2R,3R)-2,3-butanediol titer of the resulting strain was increased by 33.9%. Then the transhydrogenase gene <jats:italic>udhA<\/jats:italic> from <jats:italic>E. coli<\/jats:italic> was expressed to provide more NADH for 2,3-butanediol synthesis, which reduced the accumulation of the main byproduct acetoin by 57.2%. Next, a mutant <jats:italic>atpG<\/jats:italic> was integrated into strain CGK3, which increased the glucose consumption rate by 10.5% and the 2,3-butanediol productivity by 10.9% in shake-flask fermentation. Through fermentation engineering, the most promising strain CGK4 produced a titer of 144.9\u00a0g\/L (2R,3R)-2,3-butanediol with a yield of 0.429\u00a0g\/g glucose and a productivity of 1.10\u00a0g\/L\/h in fed-batch fermentation. The optical purity of the resulting (2R,3R)-2,3-butanediol surpassed 98%.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>To the best of our knowledge, this is the highest titer of optically pure (2R,3R)-2,3-butanediol achieved by GRAS strains, and the result has demonstrated that <jats:italic>C. glutamicum<\/jats:italic> is a competitive candidate for (2R,3R)-2,3-butanediol production.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12934-022-01875-5","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T04:08:54Z","timestamp":1658722134000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Metabolic engineering of Corynebacterium glutamicum for efficient production of optically pure (2R,3R)-2,3-butanediol"],"prefix":"10.1186","volume":"21","author":[{"given":"Mengyun","family":"Kou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,25]]},"reference":[{"key":"1875_CR1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/bit.260220613","volume":"22","author":"MC Flickinger","year":"1980","unstructured":"Flickinger MC. 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