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In recent years, the chemical methods have been predominantly employed for synthesizing 1,5-PDO. However, with the increasing emphasis on environmentally friendly production, it has been a growing interest in the biosynthesis of 1,5-PDO. Due to the limited availability of only three reported feasible biosynthesis pathways, we developed a new biosynthetic pathway to form a cell factory in\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      to produce 1,5-PDO.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In this study, we reported an artificial pathway for the synthesis of 1,5-PDO from lysine with an integrated cofactor and co-substrate recycling and also evaluated its feasibility in\n                      <jats:italic>E.coli<\/jats:italic>\n                      . To get through the pathway, we first screened aminotransferases originated from different organisms to identify the enzyme that could successfully transfer two amines from cadaverine, and thus GabT from\n                      <jats:italic>E. coli<\/jats:italic>\n                      was characterized. It was then cascaded with lysine decarboxylase and alcohol dehydrogenase from\n                      <jats:italic>E. coli<\/jats:italic>\n                      to achieve the whole-cell production of 1,5-PDO from lysine. To improve the whole-cell activity for 1,5-PDO production, we employed a protein scaffold of EutM for GabT assembly and glutamate dehydrogenase was also validated for the recycling of NADPH and \u03b1-ketoglutaric acid (\u03b1-KG). After optimizing the cultivation and bioconversion conditions, the titer of 1,5-PDO reached 4.03\u00a0mM.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We established a novel pathway for 1,5-PDO production through two consecutive transamination reaction from cadaverine, and also integrated cofactor and co-substrate recycling system, which provided an alternative option for the biosynthesis of 1,5-PDO.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12934-024-02408-y","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T10:01:41Z","timestamp":1714989701000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["An integrated cofactor and co-substrate recycling pathway for the biosynthesis of 1,5-pentanediol"],"prefix":"10.1186","volume":"23","author":[{"given":"Wenfeng","family":"Hua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kequan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"2408_CR1","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.eurpolymj.2018.11.029","volume":"110","author":"J Lu","year":"2019","unstructured":"Lu J, Zhou LZ, Xie HZ, et al. 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