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Additionally, it showed 2.8-fold increased photostability compared to pyrrolnitrin, a commercially available HPD with antimicrobial activity. For microbial production of APRN, we first engineered anthranilate phosphoribosyltransferase encoded by <jats:italic>trpD<\/jats:italic> from <jats:italic>Corynebacterium glutamicum<\/jats:italic>, resulting in a TrpD<jats:sup>A162D<\/jats:sup> mutation that exhibits feedback-resistant against <jats:sc>l<\/jats:sc>-tryptophan and higher substrate affinity compared to wild-type TrpD. Plasmid-borne expression of <jats:italic>trpD<\/jats:italic><jats:sup>A162D<\/jats:sup> in <jats:italic>C. glutamicum<\/jats:italic> TP851 strain with two copies of <jats:italic>trpD<\/jats:italic><jats:sup>A162D<\/jats:sup> in the genome led to the production of 3.1\u00a0g\/L <jats:sc>l<\/jats:sc>-tryptophan in flask culture. Subsequent step for <jats:sc>l<\/jats:sc>-tryptophan chlorination into 7-chloro-<jats:sc>l<\/jats:sc>-tryptophan was achieved by introducing diverse sources of genes encoding tryptophan 7-halogenase (PrnA or RebH) and flavin reductase (Fre, PrnF, or RebF). The combined expression of <jats:italic>prnA<\/jats:italic> from <jats:italic>Serratia grimesii<\/jats:italic> or <jats:italic>Serratia plymuthica<\/jats:italic> with flavin reductase gene from <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Pseudomonas fluorescens<\/jats:italic>, or <jats:italic>Lechevalieria aerocolonigenes<\/jats:italic> yielded higher production of 7-chloro-<jats:sc>l<\/jats:sc>-tryptophan in comparison to other sets of two-component systems. In the next step, production of putative monodechloroaminopyrrolnitrin (MDAP) from 7-chloro-<jats:sc>l<\/jats:sc>-tryptophan was achieved through the expression of <jats:italic>prnB<\/jats:italic> encoding MDAP synthase from <jats:italic>S. plymuthica<\/jats:italic> or <jats:italic>P. fluorescens<\/jats:italic>. Finally, an artificial APRN biosynthetic pathway was constructed by simultaneously expressing genes coding for tryptophan 7-halogenase, flavin reductase, MDAP synthase, and MDAP halogenase (PrnC) from different microbial sources within the <jats:sc>l<\/jats:sc>-tryptophan-producing TP851 strain. As <jats:italic>prnC<\/jats:italic> from <jats:italic>S. grimesii<\/jats:italic> or <jats:italic>S. plymuthica<\/jats:italic> was introduced into the host strain, which carried plasmids expressing <jats:italic>prnA<\/jats:italic> from <jats:italic>S. plymuthica<\/jats:italic>, <jats:italic>fre<\/jats:italic> from <jats:italic>E. coli<\/jats:italic>, and <jats:italic>prnB<\/jats:italic> from <jats:italic>S. plymuthica<\/jats:italic>, APN3639 and APN3638 accumulated 29.5\u00a0mg\/L and 28.1\u00a0mg\/L of APRN in the culture broth. This study represents the first report on the fermentative APRN production by metabolically engineered <jats:italic>C. glutamicum<\/jats:italic>.<\/jats:p>","DOI":"10.1186\/s12934-024-02424-y","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T05:01:58Z","timestamp":1716440518000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Fermentative aminopyrrolnitrin production by metabolically engineered Corynebacterium glutamicum"],"prefix":"10.1186","volume":"23","author":[{"given":"Virginia Ryandini Melati","family":"Putri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Hee","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Young","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Hyang","family":"Kwak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theavita Chatarina","family":"Mariyes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Kerbs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker F.","family":"Wendisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Jeong","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Ok","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Ho","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,23]]},"reference":[{"key":"2424_CR1","doi-asserted-by":"publisher","first-page":"8153","DOI":"10.1128\/JB.00966-06","volume":"188","author":"K Abdurachim","year":"2006","unstructured":"Abdurachim K, Ellis HR. 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