{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:38:44Z","timestamp":1788237524132,"version":"build-2803163510"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0901500"],"award-info":[{"award-number":["2018YFA0901500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"DOI":"10.1186\/s12934-024-02525-8","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T02:02:23Z","timestamp":1727661743000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["De novo biosynthesis of \u03b2-Arbutin in Komagataella phaffii based on metabolic engineering strategies"],"prefix":"10.1186","volume":"23","author":[{"given":"Jiashuo","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengguang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunting","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangyan","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"issue":"3","key":"2525_CR1","doi-asserted-by":"publisher","first-page":"281","DOI":"10.3969\/j.Issn.1001-5949.2008.03.050","volume":"30","author":"Xu Guo Jing","year":"2008","unstructured":"Guo Jing Xu, Ping JL. Research progress of arbutin. Ningxia Med J. 2008;30(3):281\u20133. https:\/\/doi.org\/10.3969\/j.Issn.1001-5949.2008.03.050.","journal-title":"Ningxia Med J"},{"issue":"4","key":"2525_CR2","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1691\/ph.2011.0785","volume":"66","author":"D-SK Dong-Seok Kim","year":"2011","unstructured":"Dong-Seok Kim D-SK, Hailan Li HL, Yun-Mi Jeong Y-MJ, et al. Arbutin inhibits TCCSUP human bladder cancer cell proliferation via up-regulation of P21. Pharmazie. 2011;66(4):306\u20139. https:\/\/doi.org\/10.1691\/ph.2011.0785.","journal-title":"Pharmazie"},{"issue":"8","key":"2525_CR3","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1007\/s00011-012-0474-2","volume":"61","author":"H-J Lee","year":"2012","unstructured":"Lee H-J, Kim K-W. Anti-inflammatory effects of arbutin in lipopolysaccharide-stimulated BV2 microglial cells. Inflamm Res. 2012;61(8):817\u201325. https:\/\/doi.org\/10.1007\/s00011-012-0474-2.","journal-title":"Inflamm Res"},{"issue":"5","key":"2525_CR4","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.1021\/acs.jafc.9b07151","volume":"68","author":"Y Shang","year":"2020","unstructured":"Shang Y, Wei W, Zhang P, Ye B-C. Engineering Yarrowia lipolytica for enhanced production of arbutin. J Agric Food Chem. 2020;68(5):1364\u201372. https:\/\/doi.org\/10.1021\/acs.jafc.9b07151.","journal-title":"J Agric Food Chem"},{"key":"2525_CR5","doi-asserted-by":"publisher","first-page":"914280","DOI":"10.3389\/fbioe.2022.914280","volume":"10","author":"K-X Xu","year":"2022","unstructured":"Xu K-X, Xue M-G, Li Z, Ye B-C, Zhang B. Recent progress on feasible strategies for arbutin production. Front Bioeng Biotechnol. 2022;10:914280. https:\/\/doi.org\/10.3389\/fbioe.2022.914280.","journal-title":"Front Bioeng Biotechnol"},{"key":"2525_CR6","doi-asserted-by":"publisher","DOI":"10.26949\/d.cnki.gblyu.2021.000334","author":"S Zhu","year":"2021","unstructured":"Zhu S. Extraction and purification of \u03b2-arbutin from pear leaves and study on the characteristics of phospholipid complex. Beijing For Univ. 2021. https:\/\/doi.org\/10.26949\/d.cnki.gblyu.2021.000334.","journal-title":"Beijing For Univ"},{"key":"2525_CR7","doi-asserted-by":"publisher","first-page":"128491","DOI":"10.1016\/j.biortech.2022.128491","volume":"369","author":"N An","year":"2023","unstructured":"An N, Xie C, Zhou S, Wang J, Sun X, Yan Y, Shen X, Yuan Q. Establishing a growth-coupled mechanism for high-yield production of \u03b2-Arbutin from glycerol in Escherichia coli. Bioresour Technol. 2023;369:128491. https:\/\/doi.org\/10.1016\/j.biortech.2022.128491.","journal-title":"Bioresour Technol"},{"issue":"1","key":"2525_CR8","doi-asserted-by":"publisher","first-page":"5564","DOI":"10.1038\/s41467-020-19390-9","volume":"11","author":"MI Espinosa","year":"2020","unstructured":"Espinosa MI, Gonzalez-Garcia RA, Valgepea K, Plan MR, Scott C, Pretorius IS, Marcellin E, Paulsen IT, Williams TC. Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae. Nat Commun. 2020;11(1):5564. https:\/\/doi.org\/10.1038\/s41467-020-19390-9.","journal-title":"Nat Commun"},{"issue":"1","key":"2525_CR9","doi-asserted-by":"publisher","first-page":"2387","DOI":"10.1038\/s41467-018-04795-4","volume":"9","author":"BM Woolston","year":"2018","unstructured":"Woolston BM, King JR, Reiter M, Van Hove B, Stephanopoulos G. Improving formaldehyde consumption drives methanol assimilation in engineered E. coli. Nat Commun. 2018;9(1):2387. https:\/\/doi.org\/10.1038\/s41467-018-04795-4.","journal-title":"Nat Commun"},{"issue":"29","key":"2525_CR10","doi-asserted-by":"publisher","first-page":"e2201711119","DOI":"10.1073\/pnas.2201711119","volume":"119","author":"P Cai","year":"2022","unstructured":"Cai P, Wu X, Deng J, Gao L, Shen Y, Yao L, Zhou YJ. Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris. Proc Natl Acad Sci. 2022;119(29):e2201711119. https:\/\/doi.org\/10.1073\/pnas.2201711119.","journal-title":"Proc Natl Acad Sci"},{"key":"2525_CR11","doi-asserted-by":"publisher","first-page":"607028","DOI":"10.3389\/fmicb.2020.607028","volume":"11","author":"L Bernauer","year":"2021","unstructured":"Bernauer L, Radkohl A, Lehmayer LGK, Emmerstorfer-Augustin A. Komagataella phaffii as emerging model organism in fundamental research. Front Microbiol. 2021;11:607028. https:\/\/doi.org\/10.3389\/fmicb.2020.607028.","journal-title":"Front Microbiol"},{"issue":"7","key":"2525_CR12","doi-asserted-by":"publisher","first-page":"2799","DOI":"10.1007\/s00253-021-11225-x","volume":"105","author":"UC Ejike","year":"2021","unstructured":"Ejike UC, Chan CJ, Lim CSY, Lim RLH. Functional evaluation of a recombinant fungal immunomodulatory protein from L. rhinocerus produced in P. pastoris and E. coli host expression systems. Appl Microbiol Biotechnol. 2021;105(7):2799\u2013813. https:\/\/doi.org\/10.1007\/s00253-021-11225-x.","journal-title":"Appl Microbiol Biotechnol"},{"issue":"12","key":"2525_CR13","doi-asserted-by":"publisher","first-page":"5301","DOI":"10.1007\/s00253-014-5732-5","volume":"98","author":"M Ahmad","year":"2014","unstructured":"Ahmad M, Hirz M, Pichler H, Schwab H. Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Appl Microbiol Biotechnol. 2014;98(12):5301\u201317. https:\/\/doi.org\/10.1007\/s00253-014-5732-5.","journal-title":"Appl Microbiol Biotechnol"},{"issue":"4","key":"2525_CR14","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1016\/j.jbiotec.2011.04.014","volume":"154","author":"A K\u00fcberl","year":"2011","unstructured":"K\u00fcberl A, Schneider J, Thallinger GG, Anderl I, Wibberg D, Hajek T, Jaenicke S, Brinkrolf K, Goesmann A, Szczepanowski R, P\u00fchler A, Schwab H, Glieder A, Pichler H. High-quality genome sequence of Pichia pastoris CBS7435. J Biotechnol. 2011;154(4):312\u201320. https:\/\/doi.org\/10.1016\/j.jbiotec.2011.04.014.","journal-title":"J Biotechnol"},{"key":"2525_CR15","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.jbiotec.2016.04.023","volume":"235","author":"L Sturmberger","year":"2016","unstructured":"Sturmberger L, Chappell T, Geier M, Krainer F, Day KJ, Vide U, Trstenjak S, Schiefer A, Richardson T, Soriaga L, Darnhofer B, Birner-Gruenberger R, Glick BS, Tolstorukov I, Cregg J, Madden K, Glieder A. Refined Pichia pastoris reference genome sequence. J Biotechnol. 2016;235:121\u201331. https:\/\/doi.org\/10.1016\/j.jbiotec.2016.04.023.","journal-title":"J Biotechnol"},{"key":"2525_CR16","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.copbio.2019.06.002","volume":"59","author":"JE Fischer","year":"2019","unstructured":"Fischer JE, Glieder A. Current advances in engineering tools for Pichia pastoris. Curr Opin Biotechnol. 2019;59:175\u201381. https:\/\/doi.org\/10.1016\/j.copbio.2019.06.002.","journal-title":"Curr Opin Biotechnol"},{"issue":"4","key":"2525_CR17","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1007\/s10529-023-03357-z","volume":"45","author":"D Qian","year":"2023","unstructured":"Qian D, Zhang C, Deng C, Zhou M, Fan L, De ZL. Novo biosynthesis of 2\u2032-fucosyllactose in engineered Pichia pastoris. Biotechnol Lett. 2023;45(4):521\u201336. https:\/\/doi.org\/10.1007\/s10529-023-03357-z.","journal-title":"Biotechnol Lett"},{"issue":"4","key":"2525_CR18","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1099\/00221287-140-4-897","volume":"140","author":"M Siebert","year":"1994","unstructured":"Siebert M, Severin K, Heide L. Formation of 4-Hydroxybenzoate in Escherichia coli characterization of the ubiC gene and its encoded enzyme chorismate pyruvate-lyase. Microbiology. 1994;140(4):897\u2013904.","journal-title":"Microbiology"},{"issue":"21","key":"2525_CR19","doi-asserted-by":"publisher","first-page":"6680","DOI":"10.1128\/jb.179.21.6680-6687.1997","volume":"179","author":"MH Eppink","year":"1997","unstructured":"Eppink MH, Boeren SA, Vervoort J, Van Berkel WJ. Purification and properties of 4-hydroxybenzoate 1-hydroxylase (decarboxylating), a novel flavin adenine dinucleotide-dependent monooxygenase from Candida parapsilosis CBS604. J Bacteriol. 1997;179(21):6680\u20137. https:\/\/doi.org\/10.1128\/jb.179.21.6680-6687.1997.","journal-title":"J Bacteriol"},{"issue":"2","key":"2525_CR20","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1002\/bit.1152","volume":"76","author":"J Arend","year":"2001","unstructured":"Arend J, Warzecha H, Hefner T, St\u00f6ckigt J. Utilizing genetically engineered bacteria to produce plant-specific glucosides: engineered bacteria to produce plant-specific glucosides. Biotechnol Bioeng. 2001;76(2):126\u201331. https:\/\/doi.org\/10.1002\/bit.1152.","journal-title":"Biotechnol Bioeng"},{"key":"2525_CR21","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.ymben.2017.06.001","volume":"42","author":"X Shen","year":"2017","unstructured":"Shen X, Wang J, Wang J, Chen Z, Yuan Q, Yan Y. High-level de novo biosynthesis of arbutin in engineered Escherichia coli. Metab Eng. 2017;42:52\u20138. https:\/\/doi.org\/10.1016\/j.ymben.2017.06.001.","journal-title":"Metab Eng"},{"issue":"1","key":"2525_CR22","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1186\/s12934-018-1022-8","volume":"17","author":"S Wang","year":"2018","unstructured":"Wang S, Fu C, Bilal M, Hu H, Wang W, Zhang X. Enhanced biosynthesis of arbutin by engineering shikimate pathway in pseudomonas chlororaphis P3. Microb Cell Factories. 2018;17(1):174. https:\/\/doi.org\/10.1186\/s12934-018-1022-8.","journal-title":"Microb Cell Factories"},{"issue":"20","key":"2525_CR23","doi-asserted-by":"publisher","first-page":"6193","DOI":"10.1007\/s00253-023-12706-x","volume":"107","author":"N An","year":"2023","unstructured":"An N, Zhou S, Chen X, Wang J, Sun X, Shen X, Yuan Q. High-yield production of \u03b2-Arbutin by identifying and eliminating byproducts formation. Appl Microbiol Biotechnol. 2023;107(20):6193\u2013204. https:\/\/doi.org\/10.1007\/s00253-023-12706-x.","journal-title":"Appl Microbiol Biotechnol"},{"key":"2525_CR24","doi-asserted-by":"publisher","unstructured":"Gibson DG Enzymatic assembly of overlapping DNA fragments. In: Methods in Enzymology, vol. 498. Elsevier, 2011. p. 349\u201361. https:\/\/doi.org\/10.1016\/B978-0-12-385120-8.00015-2.","DOI":"10.1016\/B978-0-12-385120-8.00015-2"},{"issue":"1","key":"2525_CR25","doi-asserted-by":"publisher","first-page":"152","DOI":"10.2144\/04361DD02","volume":"36","author":"S Wu","year":"2004","unstructured":"Wu S, Letchworth GJ. High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol. Biotechniques. 2004;36(1):152\u20134. https:\/\/doi.org\/10.2144\/04361DD02.","journal-title":"Biotechniques"},{"issue":"1","key":"2525_CR26","doi-asserted-by":"publisher","first-page":"11113","DOI":"10.1038\/s41598-017-11494-5","volume":"7","author":"C Li","year":"2017","unstructured":"Li C, Lin Y, Zheng X, Yuan Q, Pang N, Liao X, Huang Y, Zhang X, Liang S. Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris. Sci Rep. 2017;7(1):11113. https:\/\/doi.org\/10.1038\/s41598-017-11494-5.","journal-title":"Sci Rep"},{"issue":"2","key":"2525_CR27","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1021\/acssynbio.1c00307","volume":"11","author":"J Gao","year":"2022","unstructured":"Gao J, Xu J, Zuo Y, Ye C, Jiang L, Feng L, Huang L, Xu Z, Lian J. Synthetic biology toolkit for marker-less integration of multigene pathways into Pichia pastoris via CRISPR\/Cas9. ACS Synth Biol. 2022;11(2):623\u201333. https:\/\/doi.org\/10.1021\/acssynbio.1c00307.","journal-title":"ACS Synth Biol"},{"issue":"6","key":"2525_CR28","doi-asserted-by":"publisher","first-page":"632","DOI":"10.3724\/SP.J.1123.2010.00632","volume":"28","author":"X Kong","year":"2010","unstructured":"Kong X, He Q, Yue A, Wu S, Li J. Determination of arbutin in apple juice concentrate by ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry: determination of arbutin in apple juice concentrate by ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry. Chin J Chromatogr. 2010;28(6):632\u20134. https:\/\/doi.org\/10.3724\/SP.J.1123.2010.00632.","journal-title":"Chin J Chromatogr"},{"key":"2525_CR29","doi-asserted-by":"publisher","unstructured":"Huxia, Chenminglong, Yangweihan, et al. Determination of arbutin in soluble microneedle patches by HPLC. J Shanxi Med Univ. 2018;49(5): 4. https:\/\/doi.org\/10.13753\/j.Issn.1007-6611.2018.05.009.","DOI":"10.13753\/j.Issn.1007-6611.2018.05.009"},{"key":"2525_CR30","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.ymben.2017.03.005","volume":"41","author":"G-Z Jiang","year":"2017","unstructured":"Jiang G-Z, Yao M-D, Wang Y, Zhou L, Song T-Q, Liu H, Xiao W-H, Yuan Y-J. Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae. Metab Eng. 2017;41:57\u201366. https:\/\/doi.org\/10.1016\/j.ymben.2017.03.005.","journal-title":"Metab Eng"},{"key":"2525_CR31","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.ymben.2020.03.011","volume":"60","author":"T Hu","year":"2020","unstructured":"Hu T, Zhou J, Tong Y, Su P, Li X, Liu Y, Liu N, Wu X, Zhang Y, Wang J, Gao L, Tu L, Lu Y, Jiang Z, Zhou YJ, Gao W, Huang L. Engineering chimeric diterpene synthases and isoprenoid biosynthetic pathways enables high-level production of miltiradiene in yeast. Metab Eng. 2020;60:87\u201396. https:\/\/doi.org\/10.1016\/j.ymben.2020.03.011.","journal-title":"Metab Eng"},{"issue":"3","key":"2525_CR32","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1073\/pnas.0337566100","volume":"100","author":"M Hartmann","year":"2003","unstructured":"Hartmann M, Schneider TR, Pfeil A, Heinrich G, Lipscomb WN, Braus GH. Evolution of feedback-inhibited \u03b2\/\u03b1 barrel isoenzymes by gene duplication and a single mutation. Proc Natl Acad Sci. 2003;100(3):862\u20137.","journal-title":"Proc Natl Acad Sci"},{"key":"2525_CR33","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.ymben.2012.10.003","volume":"15","author":"KA Curran","year":"2013","unstructured":"Curran KA, Leavitt JM, Karim AS, Alper HS. Metabolic engineering of muconic acid production in Saccharomyces cerevisiae. Metab Eng. 2013;15:55\u201366. https:\/\/doi.org\/10.1016\/j.ymben.2012.10.003.","journal-title":"Metab Eng"},{"key":"2525_CR34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ymben.2015.08.007","volume":"32","author":"M Li","year":"2015","unstructured":"Li M, Kildegaard KR, Chen Y, Rodriguez A, Borodina I, De NJ. Novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae. Metab Eng. 2015;32:1\u201311. https:\/\/doi.org\/10.1016\/j.ymben.2015.08.007.","journal-title":"Metab Eng"},{"issue":"7","key":"2525_CR35","doi-asserted-by":"publisher","first-page":"2368","DOI":"10.1002\/anie.201509653","volume":"55","author":"M Suastegui","year":"2016","unstructured":"Suastegui M, Matthiesen JE, Carraher JM, Hernandez N, Rodriguez Quiroz N, Okerlund A, Cochran EW, Shao Z, Tessonnier J. Combining metabolic engineering and electrocatalysis: application to the production of polyamides from sugar. Angew Chem Int Ed. 2016;55(7):2368\u201373. https:\/\/doi.org\/10.1002\/anie.201509653.","journal-title":"Angew Chem Int Ed"},{"issue":"2","key":"2525_CR36","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1042\/bj2460375","volume":"246","author":"K Duncan","year":"1987","unstructured":"Duncan K, Edwards RM, Coggins JR. The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains. Biochem J. 1987;246(2):375\u201386. https:\/\/doi.org\/10.1042\/bj2460375.","journal-title":"Biochem J"},{"issue":"7","key":"2525_CR37","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1007\/s10529-017-2322-5","volume":"39","author":"J Mao","year":"2017","unstructured":"Mao J, Liu Q, Song X, Wang H, Feng H, Xu H, Qiao M. Combinatorial analysis of enzymatic bottlenecks of L-tyrosine pathway by p-coumaric acid production in Saccharomyces cerevisiae. Biotechnol Lett. 2017;39(7):977\u201382. https:\/\/doi.org\/10.1007\/s10529-017-2322-5.","journal-title":"Biotechnol Lett"},{"issue":"4","key":"2525_CR38","doi-asserted-by":"publisher","first-page":"2049","DOI":"10.1021\/acs.jafc.2c08228","volume":"71","author":"G Luo","year":"2023","unstructured":"Luo G, Lin Y, Chen S, Xiao R, Zhang J, Li C, Sinskey AJ, Ye L, Liang S. Overproduction of patchoulol in metabolically engineered Komagataella phaffii. J Agric Food Chem. 2023;71(4):2049\u201358. https:\/\/doi.org\/10.1021\/acs.jafc.2c08228.","journal-title":"J Agric Food Chem"},{"issue":"10","key":"2525_CR39","doi-asserted-by":"publisher","first-page":"2961","DOI":"10.1021\/acssynbio.3c00294","volume":"12","author":"X Zhang","year":"2023","unstructured":"Zhang X, Chen S, Lin Y, Li W, Wang D, Ruan S, Yang Y, Liang S. Metabolic engineering of Pichia pastoris for high-level production of lycopene. ACS Synth Biol. 2023;12(10):2961\u201372. https:\/\/doi.org\/10.1021\/acssynbio.3c00294.","journal-title":"ACS Synth Biol"},{"issue":"12","key":"2525_CR40","doi-asserted-by":"publisher","first-page":"2676","DOI":"10.1002\/bit.26037","volume":"113","author":"M Su\u00e1stegui","year":"2016","unstructured":"Su\u00e1stegui M, Guo W, Feng X, Shao Z. Investigating strain dependency in the production of aromatic compounds in Saccharomyces cerevisiae. Biotechnol Bioeng. 2016;113(12):2676\u201385. https:\/\/doi.org\/10.1002\/bit.26037.","journal-title":"Biotechnol Bioeng"},{"issue":"6","key":"2525_CR41","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1007\/s00253-005-0252-y","volume":"69","author":"M Ikeda","year":"2006","unstructured":"Ikeda M. Towards bacterial strains overproducing L-tryptophan and other aromatics by metabolic engineering. Appl Microbiol Biotechnol. 2006;69(6):615\u201326. https:\/\/doi.org\/10.1007\/s00253-005-0252-y.","journal-title":"Appl Microbiol Biotechnol"},{"key":"2525_CR42","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1038\/nbt0596-620","volume":"14","author":"N Flores","year":"1996","unstructured":"Flores N, Xiao J, Berry A, Bolivar F, Valle F. Pathway engineering for the production of aromatic compounds in Escherichia coli. Nat Biotechnol. 1996;14:620\u20133.","journal-title":"Nat Biotechnol"},{"issue":"11","key":"2525_CR43","doi-asserted-by":"publisher","first-page":"3903","DOI":"10.1128\/aem.60.11.3903-3908.1994","volume":"60","author":"R Patnaik","year":"1994","unstructured":"Patnaik R, Liao JC. Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield. Appl Environ Microbiol. 1994;60(11):3903\u20138. https:\/\/doi.org\/10.1128\/aem.60.11.3903-3908.1994.","journal-title":"Appl Environ Microbiol"},{"key":"2525_CR44","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.ymben.2017.06.008","volume":"42","author":"M Su\u00e1stegui","year":"2017","unstructured":"Su\u00e1stegui M, Yu Ng C, Chowdhury A, Sun W, Cao M, House E, Maranas CD, Shao Z. Multilevel engineering of the upstream module of aromatic amino acid biosynthesis in Saccharomyces cerevisiae for high production of polymer and drug precursors. Metab Eng. 2017;42:134\u201344. https:\/\/doi.org\/10.1016\/j.ymben.2017.06.008.","journal-title":"Metab Eng"},{"key":"2525_CR45","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.ymben.2017.05.002","volume":"42","author":"F Carly","year":"2017","unstructured":"Carly F, Vandermies M, Telek S, Steels S, Thomas S, Nicaud J-M, Fickers P. Enhancing erythritol productivity in yarrowia lipolytica using metabolic engineering. Metab Eng. 2017;42:19\u201324. https:\/\/doi.org\/10.1016\/j.ymben.2017.05.002.","journal-title":"Metab Eng"},{"key":"2525_CR46","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.ymben.2017.01.012","volume":"40","author":"M Deaner","year":"2017","unstructured":"Deaner M, Alper HS. Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae. Metab Eng. 2017;40:14\u201322. https:\/\/doi.org\/10.1016\/j.ymben.2017.01.012.","journal-title":"Metab Eng"},{"issue":"2","key":"2525_CR47","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1111\/j.1432-1033.1995.0525h.x","volume":"230","author":"GA Sprenger","year":"1995","unstructured":"Sprenger GA, Sch\u00f6rken U, Sprenger G, Sahm H. Transketolase a of Escherichia coli K12: purification and properties of the enzyme from recombinant strains. Eur J Biochem. 1995;230(2):525\u201332. https:\/\/doi.org\/10.1111\/j.1432-1033.1995.0525h.x.","journal-title":"Eur J Biochem"},{"issue":"1","key":"2525_CR48","doi-asserted-by":"publisher","first-page":"4976","DOI":"10.1038\/s41467-019-12961-5","volume":"10","author":"Q Liu","year":"2019","unstructured":"Liu Q, Yu T, Li X, Chen Y, Campbell K, Nielsen J, Chen Y. Rewiring carbon metabolism in yeast for high level production of aromatic chemicals. Nat Commun. 2019;10(1):4976. https:\/\/doi.org\/10.1038\/s41467-019-12961-5.","journal-title":"Nat Commun"},{"issue":"9","key":"2525_CR49","doi-asserted-by":"publisher","first-page":"5867","DOI":"10.1002\/jcp.29583","volume":"235","author":"M Karbalaei","year":"2020","unstructured":"Karbalaei M, Rezaee SA, Farsiani H. Pichia pastoris: a highly successful expression system for optimal synthesis of heterologous proteins. J Cell Physiol. 2020;235(9):5867\u201381. https:\/\/doi.org\/10.1002\/jcp.29583.","journal-title":"J Cell Physiol"},{"issue":"4","key":"2525_CR50","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1016\/j.cell.2020.07.010","volume":"182","author":"FY-H Chen","year":"2020","unstructured":"Chen FY-H, Jung H-W, Tsuei C-Y, Liao JC. Converting Escherichia coli to a synthetic methylotroph growing solely on methanol. Cell. 2020;182(4):933-946.e14. https:\/\/doi.org\/10.1016\/j.cell.2020.07.010.","journal-title":"Cell"},{"issue":"1","key":"2525_CR51","doi-asserted-by":"publisher","first-page":"2142","DOI":"10.1038\/s41467-019-09848-w","volume":"10","author":"A Cravens","year":"2019","unstructured":"Cravens A, Payne J, Smolke CD. Synthetic biology strategies for microbial biosynthesis of plant natural products. Nat Commun. 2019;10(1):2142. https:\/\/doi.org\/10.1038\/s41467-019-09848-w.","journal-title":"Nat Commun"},{"issue":"3","key":"2525_CR52","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/S1369-5266(03)00039-6","volume":"6","author":"NJ Kruger","year":"2003","unstructured":"Kruger NJ, Von Schaewen A. The oxidative pentose phosphate pathway: structure and organisation. Curr Opin Plant Biol. 2003;6(3):236\u201346. https:\/\/doi.org\/10.1016\/S1369-5266(03)00039-6.","journal-title":"Curr Opin Plant Biol"}],"container-title":["Microbial Cell Factories"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-024-02525-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12934-024-02525-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-024-02525-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T02:03:52Z","timestamp":1727661832000},"score":1,"resource":{"primary":{"URL":"https:\/\/microbialcellfactories.biomedcentral.com\/articles\/10.1186\/s12934-024-02525-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,30]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["2525"],"URL":"https:\/\/doi.org\/10.1186\/s12934-024-02525-8","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4709521\/v1","asserted-by":"object"}]},"ISSN":["1475-2859"],"issn-type":[{"value":"1475-2859","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,30]]},"assertion":[{"value":"9 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 September 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"261"}}