{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:15:00Z","timestamp":1792804500692,"version":"4.5.0"},"reference-count":65,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["J7-4418"],"award-info":[{"award-number":["J7-4418"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["J7-4418"],"award-info":[{"award-number":["J7-4418"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["P4-0127"],"award-info":[{"award-number":["P4-0127"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research and Innovation Agency","doi-asserted-by":"crossref","award":["J7-4418"],"award-info":[{"award-number":["J7-4418"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"DOI":"10.1186\/s12934-024-02612-w","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T22:15:40Z","timestamp":1734560140000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion"],"prefix":"10.1186","volume":"23","author":[{"given":"Spase","family":"Stojanov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina Vida","family":"Plavec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0160pela","family":"Zupan\u010di\u010d","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ale\u0161","family":"Berlec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,19]]},"reference":[{"key":"2612_CR1","doi-asserted-by":"publisher","first-page":"81","DOI":"10.3389\/fphys.2015.00081","volume":"6","author":"MI Petrova","year":"2015","unstructured":"Petrova MI, Lievens E, Malik S, Imholz N, Lebeer S. Lactobacillus species as biomarkers and agents that can promote various aspects of vaginal health. Front Physiol. 2015;6:81.","journal-title":"Front Physiol"},{"key":"2612_CR2","doi-asserted-by":"publisher","first-page":"e3366","DOI":"10.7717\/peerj.3366","volume":"5","author":"ZS Ma","year":"2017","unstructured":"Ma ZS, Li L. Quantifying the human vaginal community state types (CSTs) with the species specificity index. PeerJ. 2017;5:e3366.","journal-title":"PeerJ"},{"key":"2612_CR3","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1186\/s12934-020-01464-4","volume":"19","author":"WJY Chee","year":"2020","unstructured":"Chee WJY, Chew SY, Than LTL. Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health. Microb Cell Fact. 2020;19:203.","journal-title":"Microb Cell Fact"},{"key":"2612_CR4","doi-asserted-by":"publisher","first-page":"2183","DOI":"10.1038\/s41564-023-01500-0","volume":"8","author":"S Lebeer","year":"2023","unstructured":"Lebeer S, Ahannach S, Gehrmann T, Wittouck S, Eilers T, Oerlemans E, Condori S, Dillen J, Spacova I, Vander Donck L, et al. A citizen-science-enabled catalogue of the vaginal microbiome and associated factors. Nat Microbiol. 2023;8:2183\u201395.","journal-title":"Nat Microbiol"},{"key":"2612_CR5","doi-asserted-by":"publisher","first-page":"419","DOI":"10.3390\/nu12020419","volume":"12","author":"A Barrientos-Duran","year":"2020","unstructured":"Barrientos-Duran A, Fuentes-Lopez A, de Salazar A, Plaza-Diaz J, Garcia F. Reviewing the composition of vaginal microbiota: inclusion of nutrition and probiotic factors in the maintenance of eubiosis. Nutrients. 2020;12:419.","journal-title":"Nutrients"},{"key":"2612_CR6","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/S1286-4579(00)00313-0","volume":"2","author":"S Boris","year":"2000","unstructured":"Boris S, Barbes C. Role played by lactobacilli in controlling the population of vaginal pathogens. Microbes Infect. 2000;2:543\u20136.","journal-title":"Microbes Infect"},{"key":"2612_CR7","doi-asserted-by":"publisher","first-page":"1212","DOI":"10.1093\/cid\/cir183","volume":"52","author":"AE Stapleton","year":"2011","unstructured":"Stapleton AE, Au-Yeung M, Hooton TM, Fredricks DN, Roberts PL, Czaja CA, Yarova-Yarovaya Y, Fiedler T, Cox M, Stamm WE. Randomized, placebo-controlled phase 2 trial of a Lactobacillus crispatus probiotic given intravaginally for prevention of recurrent urinary tract infection. Clin Infect Diseases: Official Publication Infect Dis Soc Am. 2011;52:1212\u20137.","journal-title":"Clin Infect Diseases: Official Publication Infect Dis Soc Am"},{"key":"2612_CR8","doi-asserted-by":"publisher","first-page":"e96659","DOI":"10.1371\/journal.pone.0096659","volume":"9","author":"JP Ghartey","year":"2014","unstructured":"Ghartey JP, Smith BC, Chen Z, Buckley N, Lo Y, Ratner AJ, Herold BC, Burk RD. Lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against Escherichia coli. PLoS ONE. 2014;9:e96659.","journal-title":"PLoS ONE"},{"key":"2612_CR9","doi-asserted-by":"publisher","first-page":"2900","DOI":"10.3389\/fmicb.2019.02900","volume":"10","author":"F Atassi","year":"2019","unstructured":"Atassi F, Pho Viet Ahn DL, Lievin-Le Moal V. Diverse expression of antimicrobial activities against bacterial vaginosis and urinary tract infection pathogens by cervicovaginal microbiota strains of Lactobacillus gasseri and Lactobacillus crispatus. Front Microbiol. 2019;10:2900.","journal-title":"Front Microbiol"},{"key":"2612_CR10","doi-asserted-by":"publisher","first-page":"3103","DOI":"10.1128\/IAI.01200-09","volume":"78","author":"RR Spurbeck","year":"2010","unstructured":"Spurbeck RR, Arvidson CG. Lactobacillus jensenii surface-associated proteins inhibit Neisseria gonorrhoeae adherence to epithelial cells. Infect Immun. 2010;78:3103\u201311.","journal-title":"Infect Immun"},{"key":"2612_CR11","first-page":"233","volume-title":"Frontiers in Clinical Drug Research: anti-infectives","author":"S Borges","year":"2015","unstructured":"Borges S, Barbosa J, Teixeira P. Drug delivery systems for vaginal infections. In: Rahman AU, editor. Frontiers in Clinical Drug Research: anti-infectives. Sharjah, United Arab Emirates: Bentham Science; 2015. pp. 233\u201358."},{"key":"2612_CR12","doi-asserted-by":"publisher","first-page":"103162","DOI":"10.1016\/j.jddst.2022.103162","volume":"70","author":"B Valamla","year":"2022","unstructured":"Valamla B, Thakor P, Phuse R, Dalvi M, Kharat P, Kumar A, Panwar D, Singh SB, Giorgia P, Mehra NK. Engineering drug delivery systems to overcome the vaginal mucosal barrier: current understanding and research agenda of mucoadhesive formulations of vaginal delivery. J Drug Deliv Sci Tec. 2022;70:103162.","journal-title":"J Drug Deliv Sci Tec"},{"key":"2612_CR13","doi-asserted-by":"publisher","first-page":"5298","DOI":"10.1021\/acs.chemrev.8b00593","volume":"119","author":"JJ Xue","year":"2019","unstructured":"Xue JJ, Wu T, Dai YQ, Xia YN. Electrospinning and Electrospun nanofibers: methods, materials, and applications. Chem Rev. 2019;119:5298\u2013415.","journal-title":"Chem Rev"},{"key":"2612_CR14","doi-asserted-by":"publisher","first-page":"130","DOI":"10.3389\/fbioe.2020.00130","volume":"8","author":"S Stojanov","year":"2020","unstructured":"Stojanov S, Berlec A. Electrospun nanofibers as carriers of microorganisms, stem cells, proteins, and nucleic acids in therapeutic and other applications. Front Bioeng Biotech. 2020;8:130.","journal-title":"Front Bioeng Biotech"},{"key":"2612_CR15","doi-asserted-by":"publisher","first-page":"1360","DOI":"10.2174\/1567201815666180723114326","volume":"15","author":"EJ Torres-Mart\u00ednez","year":"2018","unstructured":"Torres-Mart\u00ednez EJ, Bravo JMC, Medina AS, Gonz\u00e1lez GLP, G\u00f3mez LJV. A summary of electrospun nanofibers as drug delivery system: drugs loaded and biopolymers used as matrices. Curr Drug Deliv. 2018;15:1360\u201374.","journal-title":"Curr Drug Deliv"},{"key":"2612_CR16","doi-asserted-by":"publisher","first-page":"20240057","DOI":"10.1515\/ntrev-2024-0057","volume":"13","author":"S Stojanov","year":"2024","unstructured":"Stojanov S, Berlec A. Smart bionanomaterials for treatment and diagnosis of inflammatory bowel disease. Nanotechnol Rev. 2024;13:20240057.","journal-title":"Nanotechnol Rev"},{"key":"2612_CR17","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.jconrel.2023.01.082","volume":"355","author":"NK Grilc","year":"2023","unstructured":"Grilc NK, Zidar A, Kocbek P, Rijavec T, Colja T, Lapanje A, Jeras M, Gobec M, Mlinari\u010d-Ras\u010dan I, Gasperlin M, et al. Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity. J Control Release. 2023;355:371\u201384.","journal-title":"J Control Release"},{"key":"2612_CR18","doi-asserted-by":"publisher","first-page":"483","DOI":"10.3390\/pharmaceutics11090483","volume":"11","author":"\u0160 Zupan\u010di\u010d","year":"2019","unstructured":"Zupan\u010di\u010d \u0160, \u0160krlec K, Kocbek P, Kristl J, Berlec A. Effects of electrospinning on the viability of ten species of lactic acid bacteria in poly(ethylene oxide) nanofibers. Pharmaceutics. 2019;11:483.","journal-title":"Pharmaceutics"},{"key":"2612_CR19","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.fbp.2021.04.016","volume":"128","author":"E Hirscha","year":"2021","unstructured":"Hirscha E, Panteaa E, Vassa P, Domj\u00e1n J, Moln\u00e1r M, Suhajda A, Andersen SK, Vigh T, Verreck G, Marosi GJ, et al. Probiotic bacteria stabilized in orally dissolving nanofibers prepared by high-speed electrospinning. Food Bioprod Process. 2021;128:84\u201394.","journal-title":"Food Bioprod Process"},{"key":"2612_CR20","doi-asserted-by":"publisher","first-page":"105563","DOI":"10.1016\/j.ejps.2020.105563","volume":"156","author":"JA Silva","year":"2021","unstructured":"Silva JA, De Gregorio PR, Rivero G, Abraham GA, Nader-Mac\u00edas MEF. Immobilization of vaginal Lactobacillus in polymeric nanofibers for its incorporation in vaginal probiotic products. Eur J Pharm Sci. 2021;156:105563.","journal-title":"Eur J Pharm Sci"},{"key":"2612_CR21","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.ejpb.2023.07.009","volume":"190","author":"F Minooei","year":"2023","unstructured":"Minooei F, Gilbert NM, Zhang L, Sarah NeCamp M, Mahmoud MY, Kyser AJ, Tyo KM, Watson WH, Patwardhan R, Lewis WG, et al. Rapid-dissolving electrospun nanofibers for intra-vaginal antibiotic or probiotic delivery. Eur J Pharm Biopharm. 2023;190:81\u201393.","journal-title":"Eur J Pharm Biopharm"},{"key":"2612_CR22","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.ejpb.2023.04.011","volume":"187","author":"MY Mahmoud","year":"2023","unstructured":"Mahmoud MY, Wesley M, Kyser A, Lewis WG, Lewis AL, Steinbach-Rankins JM, Frieboes HB. Lactobacillus crispatus-loaded electrospun fibers yield viable and metabolically active bacteria that kill Gardnerella in vitro. Eur J Pharm Biopharm. 2023;187:68\u201375.","journal-title":"Eur J Pharm Biopharm"},{"key":"2612_CR23","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.3390\/pharmaceutics14061155","volume":"14","author":"S Stojanov","year":"2022","unstructured":"Stojanov S, Kristl J, Zupancic S, Berlec A. Influence of excipient composition on survival of vaginal lactobacilli in electrospun nanofibers. Pharmaceutics. 2022;14:1155.","journal-title":"Pharmaceutics"},{"key":"2612_CR24","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1590\/S1517-838246320140556","volume":"46","author":"XF Han","year":"2015","unstructured":"Han XF, Wang L, Li W, Li BB, Yang YX, Yan HL, Qu L, Chen YL. Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats. Braz J Microbiol. 2015;46:849\u201354.","journal-title":"Braz J Microbiol"},{"key":"2612_CR25","doi-asserted-by":"publisher","first-page":"1748","DOI":"10.3389\/fmicb.2019.01748","volume":"10","author":"ML Mohedano","year":"2019","unstructured":"Mohedano ML, Hern\u00e1ndez-Recio S, Y\u00e9pez A, Requena T, Mart\u00ednez-Cuesta MC, Pel\u00e1ez C, Russo P, LeBlanc JG, Spano G, Aznar R, et al. Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling. Front Microbiol. 2019;10:1748.","journal-title":"Front Microbiol"},{"key":"2612_CR26","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1111\/1751-7915.12872","volume":"11","author":"I Spacova","year":"2018","unstructured":"Spacova I, Lievens E, Verhoeven T, Steenackers H, Vanderleyden J, Lebeer S, Petrova MI. Expression of fluorescent proteins in Lactobacillus rhamnosus to study host-microbe and microbe-microbe interactions. Microb Biotechnol. 2018;11:317\u201331.","journal-title":"Microb Biotechnol"},{"key":"2612_CR27","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1186\/s12934-015-0376-4","volume":"14","author":"A Berlec","year":"2015","unstructured":"Berlec A, Zavr\u0161nik J, Butinar M, Turk B, \u0160trukelj B. In vivo imaging of Lactococcus lactis, Lactobacillus plantarum and Escherichia coli expressing infrared fluorescent protein in mice. Microb Cell Fact. 2015;14:181.","journal-title":"Microb Cell Fact"},{"key":"2612_CR28","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.ejpb.2019.01.013","volume":"136","author":"K \u0160krlec","year":"2019","unstructured":"\u0160krlec K, Zupan\u010di\u010d S, Mihevc SP, Kocbek P, Kristl J, Berlec A. Development of electrospun nanofibers that enable high loading and long-term viability of probiotics. Eur J Pharm Biopharm. 2019;136:108\u201319.","journal-title":"Eur J Pharm Biopharm"},{"key":"2612_CR29","doi-asserted-by":"publisher","first-page":"13631","DOI":"10.3390\/ijms222413631","volume":"22","author":"S Stojanov","year":"2021","unstructured":"Stojanov S, Plavec TV, Kristl J, Zupan\u010di\u010d S, Berlec A. Engineering of vaginal lactobacilli to express fluorescent proteins enables the analysis of their mixture in nanofibers. Int J Mol Sci. 2021;22:13631.","journal-title":"Int J Mol Sci"},{"key":"2612_CR30","doi-asserted-by":"crossref","unstructured":"Riggs P. Fusion protein. In: Maloy KH, editor. Brenner\u2019s Encyclopedia of Genetics. Elsevier; 2013. pp. 134\u20135.","DOI":"10.1016\/B978-0-12-374984-0.00565-9"},{"key":"2612_CR31","doi-asserted-by":"publisher","first-page":"147","DOI":"10.5851\/kosfa.2017.37.1.147","volume":"37","author":"GS Moon","year":"2017","unstructured":"Moon GS, Narbad A. Monitoring of bioluminescent Lactobacillus plantarum in a complex food matrix. Korean J Food Sci An. 2017;37:147\u201352.","journal-title":"Korean J Food Sci An"},{"key":"2612_CR32","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1186\/s12866-018-1315-4","volume":"18","author":"WF Van Zyl","year":"2018","unstructured":"Van Zyl WF, Deane SM, Dicks LMT. In vivo bioluminescence imaging of the spatial and temporal colonization of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA in the intestinal tract of mice. BMC Microbiol. 2018;18:171.","journal-title":"BMC Microbiol"},{"key":"2612_CR33","doi-asserted-by":"publisher","first-page":"3570","DOI":"10.1128\/AEM.68.7.3570-3574.2002","volume":"68","author":"R Oozeer","year":"2002","unstructured":"Oozeer R, Goupil-Feuillerat N, Alpert CA, van de Guchte M, Anba J, Mengaud J, Corthier G. Lactobacillus casei is able to survive and initiate protein synthesis during its transit in the digestive tract of human flora-associated mice. Appl Environ Microb. 2002;68:3570\u20134.","journal-title":"Appl Environ Microb"},{"key":"2612_CR34","doi-asserted-by":"publisher","first-page":"5344","DOI":"10.1128\/AEM.01042-15","volume":"81","author":"C Daniel","year":"2015","unstructured":"Daniel C, Poiret S, Dennin V, Boutillier D, Lacorre DA, Foligne B, Pot B. Dual-color bioluminescence imaging for simultaneous monitoring of the intestinal persistence of Lactobacillus plantarum and Lactococcus lactis in living mice. Appl Environ Microbiol. 2015;81:5344\u20139.","journal-title":"Appl Environ Microbiol"},{"key":"2612_CR35","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1021\/acs.bioconjchem.6b00112","volume":"27","author":"CG England","year":"2016","unstructured":"England CG, Ehlerding EB, Cai WB. NanoLuc: a small luciferase is brightening up the field of bioluminescence. Bioconjug Chem. 2016;27:1175\u201387.","journal-title":"Bioconjug Chem"},{"key":"2612_CR36","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.jbiosc.2023.12.003","volume":"137","author":"C Matsuyama","year":"2024","unstructured":"Matsuyama C, Seike T, Okahashi N, Niide T, Hara KY, Hirono-Hara Y, Ishii J, Shimizu H, Toya Y, Matsuda F. Metabolome analysis of metabolic burden in Escherichia coli caused by overexpression of green fluorescent protein and delta-rhodopsin. J Biosci Bioeng. 2024;137:187\u201394.","journal-title":"J Biosci Bioeng"},{"key":"2612_CR37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/19490976.2021.1897374","volume":"13","author":"S Salome-Desnoulez","year":"2021","unstructured":"Salome-Desnoulez S, Poiret S, Foligne B, Muharram G, Peucelle V, Lafont F, Daniel C. Persistence and dynamics of fluorescent Lactobacillus plantarum in the healthy versus inflamed gut. Gut Microbes. 2021;13:1\u201316.","journal-title":"Gut Microbes"},{"key":"2612_CR38","doi-asserted-by":"publisher","first-page":"66682","DOI":"10.1039\/C6RA13172G","volume":"6","author":"S Kadkhodaei","year":"2016","unstructured":"Kadkhodaei S, Memari HR, Abbasiliasi S, Rezaei MA, Movahedi A, Shun TJ, Bin Ariff A. Multiple overlap extension PCR (MOE-PCR): an effective technical shortcut to high throughput synthetic biology. RSC Adv. 2016;6:66682\u201394.","journal-title":"RSC Adv"},{"key":"2612_CR39","doi-asserted-by":"publisher","first-page":"636421","DOI":"10.3389\/fmicb.2021.636421","volume":"12","author":"A Berlec","year":"2021","unstructured":"Berlec A, Janez N, Sternisa M, Klan\u010dnik A, Saboti\u0107 J. Expression of NanoLuc luciferase in Listeria innocua for development of biofilm assay. Front Microbiol. 2021;12:636421.","journal-title":"Front Microbiol"},{"key":"2612_CR40","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1007\/s00253-005-0107-6","volume":"68","author":"I Mierau","year":"2005","unstructured":"Mierau I, Kleerebezem M. 10 years of the nisin-controlled gene expression system (NICE) in. Appl Microbiol Biot. 2005;68:705\u201317.","journal-title":"Appl Microbiol Biot"},{"key":"2612_CR41","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/0168-1605(94)90157-0","volume":"23","author":"J Baranyi","year":"1994","unstructured":"Baranyi J, Roberts TA. A dynamic approach to predicting bacterial growth in food. Int J Food Microbiol. 1994;23:277\u201394.","journal-title":"Int J Food Microbiol"},{"key":"2612_CR42","first-page":"614","volume":"76","author":"S Stojanov","year":"2021","unstructured":"Stojanov S, Ravnikar M, Berlec A, Kreft S. Interaction between silver fir (Abies alba) wood water extract and lactobacilli. Pharmazie. 2021;76:614\u20137.","journal-title":"Pharmazie"},{"key":"2612_CR43","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/S0167-7012(00)00241-4","volume":"44","author":"B Herigstad","year":"2001","unstructured":"Herigstad B, Hamilton M, Heersink J. How to optimize the drop plate method for enumerating bacteria. J Microbiol Meth. 2001;44:121\u20139.","journal-title":"J Microbiol Meth"},{"key":"2612_CR44","doi-asserted-by":"publisher","first-page":"foz008","DOI":"10.1093\/femsyr\/foz008","volume":"19","author":"PR De Gregorio","year":"2019","unstructured":"De Gregorio PR, Silva JA, Marchesi A, Nader-Macias MEF. Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model. FEMS Yeast Res. 2019;19:foz008.","journal-title":"FEMS Yeast Res"},{"key":"2612_CR45","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.1111\/1751-7915.13907","volume":"14","author":"TV Plavec","year":"2021","unstructured":"Plavec TV, Mitrovi\u0107 A, Nanut MP, \u0160trukelj B, Kos J, Berlec A. Targeting of fluorescent Lactococcus lactis to colorectal cancer cells through surface display of tumour-antigen binding proteins. Microb Biotechnol. 2021;14:2227\u201340.","journal-title":"Microb Biotechnol"},{"key":"2612_CR46","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1128\/AEM.02006-10","volume":"77","author":"DP Stephenson","year":"2011","unstructured":"Stephenson DP, Moore RJ, Allison GE. Transformation of, and heterologous protein expression in, Lactobacillus agilis and Lactobacillus vaginalis isolates from the chicken gastrointestinal tract. Appl Environ Microbiol. 2011;77:220\u20138.","journal-title":"Appl Environ Microbiol"},{"key":"2612_CR47","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1093\/ps\/83.1.45","volume":"83","author":"SS Beasley","year":"2004","unstructured":"Beasley SS, Takala TM, Reunanen J, Apajalahti J, Saris PE. Characterization and electrotransformation of Lactobacillus crispatus isolated from chicken crop and intestine. Poult Sci. 2004;83:45\u20138.","journal-title":"Poult Sci"},{"key":"2612_CR48","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.3390\/antiox12051055","volume":"12","author":"E Miko","year":"2023","unstructured":"Miko E, Barakonyi A. The role of hydrogen-peroxide (H2O2) produced by vaginal microbiota in female reproductive health. Antioxidants. 2023;12:1055.","journal-title":"Antioxidants"},{"key":"2612_CR49","doi-asserted-by":"publisher","first-page":"5223","DOI":"10.1021\/acs.macromol.9b00691","volume":"52","author":"E Josef","year":"2019","unstructured":"Josef E, Guterman R. Designing solutions for electrospinning of poly(ionic liquid)s. Macromolecules. 2019;52:5223\u201330.","journal-title":"Macromolecules"},{"key":"2612_CR50","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/S0531-5131(02)00246-7","volume":"1233","author":"FI Tsuji","year":"2002","unstructured":"Tsuji FI. Oxidation of a cyclic tripeptide by molecular oxygen and the development of fluorescence in the Aequorea green fluorescent protein. Int Congr Ser. 2002;1233:37\u201344.","journal-title":"Int Congr Ser"},{"key":"2612_CR51","doi-asserted-by":"crossref","unstructured":"Gao YX, Li HY, Liu J. Direct writing of flexible electronics through room temperature liquid metal ink. PLoS ONE. 2012;7(9).","DOI":"10.1371\/journal.pone.0045485"},{"key":"2612_CR52","first-page":"1","volume":"5","author":"A Talwalkar","year":"2004","unstructured":"Talwalkar A, Kailasapathy K. The role of oxygen in the viability of probiotic bacteria with reference to L. Acidophilus and Bifidobacterium spp. Curr Issues Intest Microbiol. 2004;5:1\u20138.","journal-title":"Curr Issues Intest Microbiol"},{"key":"2612_CR53","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/B978-141604208-2.10007-7","volume-title":"Comprehensive Cytopathology","author":"PK Gupta","year":"2008","unstructured":"Gupta PK, McGrath C, Microbiology. Inflammation, and viral infections. In: Bibbo M, Wilbur D, editors. Comprehensive Cytopathology. Amstedram: Elsevier; 2008. pp. 91\u2013129."},{"key":"2612_CR54","unstructured":"Kairys N, Carlson K, Garg M. Bacterial vaginosis. StatPearls. 2024."},{"key":"2612_CR55","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1016\/j.biotechadv.2011.12.005","volume":"30","author":"F Silva","year":"2012","unstructured":"Silva F, Queiroz JA, Domingues FC. Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli. Biotechnol Adv. 2012;30:691\u2013708.","journal-title":"Biotechnol Adv"},{"key":"2612_CR56","doi-asserted-by":"publisher","first-page":"297","DOI":"10.3390\/microorganisms8020297","volume":"8","author":"TV Plavec","year":"2020","unstructured":"Plavec TV, Berlec A. Safety aspects of genetically modified lactic acid bacteria. Microorganisms. 2020;8:297.","journal-title":"Microorganisms"},{"key":"2612_CR57","doi-asserted-by":"publisher","first-page":"354","DOI":"10.3390\/microorganisms8030354","volume":"8","author":"I Yasmin","year":"2020","unstructured":"Yasmin I, Saeed M, Khan WA, Khaliq A, Chughtai MFJ, Iqbal R, Tehseen S, Naz S, Liaqat A, Mehmood T, et al. In vitro probiotic potential and safety evaluation (hemolytic, cytotoxic activity) of Bifidobacterium strains isolated from raw camel milk. Microorganisms. 2020;8:354.","journal-title":"Microorganisms"},{"key":"2612_CR58","doi-asserted-by":"crossref","unstructured":"Lea T. Caco-2 cell line. In: Verhoeckx K, Cotter P, Lopez-Exposito I, Kleiveland C, Lea T, Mackie A, Requena T, Swiatecka D, Wichers H, editors. The impact of food bioactives on health: in vitro and ex vivo models. 2015. pp. 103\u2013111.","DOI":"10.1007\/978-3-319-16104-4_10"},{"key":"2612_CR59","doi-asserted-by":"publisher","first-page":"123696","DOI":"10.1016\/j.ijpharm.2023.123696","volume":"650","author":"A Zidar","year":"2024","unstructured":"Zidar A, Zupan\u010di\u010d S, Kristl J, Jeras M. Development of a novel in vitro cell model for evaluation of nanofiber mats immunogenicity. Int J Pharm. 2024;650:123696.","journal-title":"Int J Pharm"},{"key":"2612_CR60","doi-asserted-by":"publisher","first-page":"3419034","DOI":"10.1155\/2020\/3419034","volume":"2020","author":"M Pohanka","year":"2020","unstructured":"Pohanka M. D-lactic acid as a metabolite: toxicology, diagnosis, and detection. Biomed Res Int. 2020;2020:3419034.","journal-title":"Biomed Res Int"},{"key":"2612_CR61","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/s12934-023-02030-4","volume":"22","author":"C Cappello","year":"2023","unstructured":"Cappello C, Acin-Albiac M, Pinto D, Polo A, Filannino P, Rinaldi F, Gobbetti M, Di Cagno R. Do nomadic lactobacilli fit as potential vaginal probiotics? The answer lies in a successful selective multi-step and scoring approach. Microb Cell Fact. 2023;22:27.","journal-title":"Microb Cell Fact"},{"key":"2612_CR62","doi-asserted-by":"publisher","first-page":"2163","DOI":"10.1016\/j.jds.2024.03.016","volume":"19","author":"P Chantanawilas","year":"2024","unstructured":"Chantanawilas P, Pahumunto N, Teanpaisan R. Aggregation and adhesion ability of various probiotic strains and Candida species: an in vitro study. J Dent Sci. 2024;19:2163\u201371.","journal-title":"J Dent Sci"},{"key":"2612_CR63","doi-asserted-by":"publisher","first-page":"1985","DOI":"10.1128\/IAI.66.5.1985-1989.1998","volume":"66","author":"S Boris","year":"1998","unstructured":"Boris S, Su\u00e1rez JE, V\u00e1zquez F, Barb\u00e9s C. Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect Immun. 1998;66:1985\u20139.","journal-title":"Infect Immun"},{"key":"2612_CR64","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1080\/17460441.2023.2160441","volume":"18","author":"B Baljinnyam","year":"2023","unstructured":"Baljinnyam B, Ronzetti M, Simeonov A. Advances in luminescence-based technologies for drug discovery. Expert Opin Drug Discov. 2023;18:25\u201335.","journal-title":"Expert Opin Drug Discov"},{"key":"2612_CR65","doi-asserted-by":"publisher","first-page":"5713","DOI":"10.1038\/s41598-022-09605-y","volume":"12","author":"TY Lai","year":"2022","unstructured":"Lai TY, Cao J, Ou-Yang P, Tsai CY, Lin CW, Chen CC, Tsai MK, Lee CY. Different methods of detaching adherent cells and their effects on the cell surface expression of Fas receptor and Fas ligand. Sci Rep. 2022;12:5713.","journal-title":"Sci Rep"}],"container-title":["Microbial Cell Factories"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-024-02612-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12934-024-02612-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-024-02612-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:09:10Z","timestamp":1734566950000},"score":1,"resource":{"primary":{"URL":"https:\/\/microbialcellfactories.biomedcentral.com\/articles\/10.1186\/s12934-024-02612-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,19]]},"references-count":65,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["2612"],"URL":"https:\/\/doi.org\/10.1186\/s12934-024-02612-w","relation":{},"ISSN":["1475-2859"],"issn-type":[{"value":"1475-2859","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,19]]},"assertion":[{"value":"17 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The manuscript is the original work of the authors who mutually agreed on submitting the manuscript.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"333"}}