{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T08:50:52Z","timestamp":1794473452111,"version":"4.9.1"},"reference-count":96,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100022025","name":"South Valley University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022025","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Nowadays, researchers are attracted to the phyco-synthesis of selenium nanoparticles (SeNPs) for biotechnological and medical applications as they possess many advantages such as safety, nutritional value, and easy biodegradation than gold, copper, and silver nanoparticles.\n                      <jats:italic>Spirulina platensis<\/jats:italic>\n                      is the preferred microalgae for SeNPs synthesis because it contains many compounds that increase their stability making them fit for biomedical treatments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The biosynthesized\n                      <jats:italic>Spirulina platensis<\/jats:italic>\n                      selenium nanoparticles (SP-SeNPs) were spherical and crystalline, with a diameter of 65\u00a0nm and a net charge of -16.7\u00a0mV. Furthermore, they were surrounded by active groups responsible for stability. The DPPH radical scavenging test assessed the antioxidant efficacy of SP-SeNPs and exposed scavenging inhibition of 79.234% at a 100\u00a0\u00b5M dosage. ABTS and H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      radical scavenging assay is dose-dependent recording IC50 of 50.69 and 116.18\u00a0\u00b5g\/ml, respectively. The antibacterial efficacy was investigated against 13 G-negative &amp; G-positive bacteria. The study demonstrated that SP-SeNPs had antibacterial and antibiofilm\u00a0efficiencies against the tested strains with MBC of 286\u2013333\u00a0\u00b5g\/ml. The highest percentages of biofilm inhibition were recorded for\n                      <jats:italic>Bacillus subtilis<\/jats:italic>\n                      and\n                      <jats:italic>Klebsiella pneumoniae,<\/jats:italic>\n                      with ratios of 78.8 and 69.9%, respectively. The prepared SP-SeNPS efficiently suppressed the tested fungi growth with MIC (350\u00a0\u00b5g\/ml) and MFCs (480\u2013950\u00a0\u00b5g\/ml). Most notably, biogenic SeNPs effectively extended the clot formation period recording 170.4 S for prothrombin time (PT) and 195.6 S for the activated partial thromboplastin time (aPTT). SP-SeNPs reduced the cell viability of breast adenocarcinoma (MCF-7) and ovarian cancer (SKOV-3) cell lines with a percentage of 17.6009% and 14.9484% at a concentration of 100 ug\/ml, respectively. Moreover, SP-SeNPs could effectively alleviate the inflammation in RAW 264.7 macrophages with a reduction percentage of 8.82% in Nitric oxide concentration.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The investigation findings reveal that SP-SeNPs are a hopeful antimicrobial, anti-tumor, anticoagulant, antioxidant, and anti-inflammatory factor that can be applied in medical cures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12934-025-02656-6","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T21:52:56Z","timestamp":1738878776000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Biogenesis, characterization, and applications of Spirulina selenium nanoparticles"],"prefix":"10.1186","volume":"24","author":[{"given":"Asmaa S.","family":"Yassein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rokaia B.","family":"Elamary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Alwaleed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"key":"2656_CR1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-22227-61","author":"S Logothetidis","year":"2012","unstructured":"Logothetidis S. Nanotechnology: principles and applications nanostructured materials and their applications. Nanosci Technol. 2012. https:\/\/doi.org\/10.1007\/978-3-642-22227-61.","journal-title":"Nanosci Technol"},{"key":"2656_CR2","first-page":"310","volume":"7","author":"S Thodeti","year":"2016","unstructured":"Thodeti S, Reddy RM, Kumar JS. Synthesis and characterization of pure and indium doped SnO2 nanoparticles by sol-gel methods. Int J Sci Eng Res. 2016;7:310\u20137.","journal-title":"Int J Sci Eng Res"},{"issue":"1","key":"2656_CR3","doi-asserted-by":"publisher","first-page":"20240063","DOI":"10.1515\/eng-2024-0063","volume":"14","author":"M Ali","year":"2024","unstructured":"Ali M. Applications of nanotechnology and nanoproduction techniques. Open Eng. 2024;14(1):20240063. https:\/\/doi.org\/10.1515\/eng-2024-0063.","journal-title":"Open Eng"},{"key":"2656_CR4","doi-asserted-by":"publisher","first-page":"26775","DOI":"10.1038\/s41598-024-77353-2","volume":"14","author":"MK Soliman","year":"2024","unstructured":"Soliman MK, Amin MAA, Nowwar AI, et al. Green synthesis of selenium nanoparticles from Cassia javanica flowers extract and their medical and agricultural applications. Sci Rep. 2024;14:26775. https:\/\/doi.org\/10.1038\/s41598-024-77353-2.","journal-title":"Sci Rep"},{"key":"2656_CR5","doi-asserted-by":"publisher","first-page":"1356284","DOI":"10.3389\/frym.2024.1356284","volume":"12","author":"N Donough","year":"2024","unstructured":"Donough N, Wepener V, Botha TL. Sizes, shapes, and types of nanomaterials. Front Young Minds. 2024;12:1356284. https:\/\/doi.org\/10.3389\/frym.2024.1356284.","journal-title":"Front Young Minds"},{"key":"2656_CR6","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1007\/s10311-023-01682-3","volume":"22","author":"AI Osman","year":"2024","unstructured":"Osman AI, Zhang Y, Farghali M, et al. Synthesis of green nanoparticles for energy, biomedical, environmental, agricultural, and food applications: a review. Environ Chem Lett. 2024;22:841\u201387. https:\/\/doi.org\/10.1007\/s10311-023-01682-3.","journal-title":"Environ Chem Lett"},{"key":"2656_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2024.106741","volume":"193","author":"X Zhu","year":"2024","unstructured":"Zhu X, Tang Q, Zhou X, Momeni MR. Antibiotic resistance and nanotechnology: a narrative review. Microb Pathog. 2024;193: 106741. https:\/\/doi.org\/10.1016\/j.micpath.2024.106741.","journal-title":"Microb Pathog"},{"issue":"38","key":"2656_CR8","doi-asserted-by":"publisher","first-page":"S33","DOI":"10.1097\/inf.0000000000002321","volume":"2019","author":"I Mack","year":"2019","unstructured":"Mack I, Bielicki J. What can we do about antimicrobial resistance? Pediatr Infect Dis J. 2019;2019(38):S33\u20138. https:\/\/doi.org\/10.1097\/inf.0000000000002321.","journal-title":"Pediatr Infect Dis J"},{"key":"2656_CR9","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404","author":"GD Brown","year":"2012","unstructured":"Brown GD, Denning DW, Gow NA, Levitz SM, Netea MG, White TC. Hidden killers: human fungal infections. Sci Transl Med. 2012. https:\/\/doi.org\/10.1126\/scitranslmed.3004404.","journal-title":"Sci Transl Med"},{"issue":"77","key":"2656_CR10","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.foodcont.2017.01.018","volume":"2017","author":"TH Nguyen","year":"2017","unstructured":"Nguyen TH, Vardhanabhuti B, Lin M, Mustapha A. Antibacterial properties of selenium nanoparticles and their toxicity to Caco-2 cells. Food Control. 2017;2017(77):17\u201324. https:\/\/doi.org\/10.1016\/j.foodcont.2017.01.018.","journal-title":"Food Control"},{"issue":"7","key":"2656_CR11","doi-asserted-by":"publisher","first-page":"760","DOI":"10.3389\/fmicb.2016.00760","volume":"2016","author":"S Scandorieiro","year":"2016","unstructured":"Scandorieiro S, Camargo LCD, Lancheros CAC, Yamada-Ogatta SF, Nakamura CV, Oliveira AGD, Andrade CGTJ, Duran N, Nakazato G, Kobayashi RKT. Synergistic and additive effect of oregano essential oil and biological silver nanoparticles against multidrug-resistant bacterial strains. Front Microbiol. 2016;2016(7):760. https:\/\/doi.org\/10.3389\/fmicb.2016.00760.","journal-title":"Front Microbiol"},{"issue":"4","key":"2656_CR12","doi-asserted-by":"publisher","first-page":"71","DOI":"10.3390\/nano6040071","volume":"6","author":"S Shamaila","year":"2016","unstructured":"Shamaila S, Zafar N, Riaz S, Sharif R, Nazir J, Naseem S. Gold Nanoparticles: an efficient antimicrobial agent against enteric bacterial human pathogen. Nanomaterials (Basel). 2016;6(4):71. https:\/\/doi.org\/10.3390\/nano6040071.","journal-title":"Nanomaterials (Basel)"},{"key":"2656_CR13","doi-asserted-by":"publisher","first-page":"5834","DOI":"10.33263\/BRIAC104.834842","volume":"10","author":"AR El-Shanshoury","year":"2020","unstructured":"El-Shanshoury AR, Darwesh OM, Sabae SZ, Awadallah MA, Hassan SH. Bio-manufacturing of selenium nanoparticles by Bacillus subtilis isolated from qarun lake and evaluation their activity for water remediation. Biointerface Res Appl Chem. 2020;10:5834\u201342. https:\/\/doi.org\/10.33263\/BRIAC104.834842.","journal-title":"Biointerface Res Appl Chem"},{"issue":"3","key":"2656_CR14","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1039\/d1ma00639","volume":"3","author":"N Bisht","year":"2022","unstructured":"Bisht N, Phalswal P, Khanna PK. Selenium nanoparticles: a review on synthesis and biomedical applications. Mater Adv. 2022;3(3):1415\u201331. https:\/\/doi.org\/10.1039\/d1ma00639.","journal-title":"Mater Adv"},{"key":"2656_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2014\/510246","volume":"2014","author":"N Kulkarni","year":"2014","unstructured":"Kulkarni N, Muddapur U. Biosynthesis of metal nanoparticles: a review. J Nanotechnol. 2014;2014:1\u20138. https:\/\/doi.org\/10.1155\/2014\/510246.","journal-title":"J Nanotechnol"},{"issue":"27","key":"2656_CR16","doi-asserted-by":"publisher","first-page":"6515","DOI":"10.1016\/j.biomaterials.2011.05.032","volume":"32","author":"L Kong","year":"2011","unstructured":"Kong L, Yuan Q, Zhu H, Li Y, Guo Q, Wang Q, Bi X, Gao X. The suppression of prostate LNCaP cancer cells growth by Selenium nanoparticles through Akt\/Mdm2\/AR controlled apoptosis. Biomaterials. 2011;32(27):6515\u201322. https:\/\/doi.org\/10.1016\/j.biomaterials.2011.05.032.","journal-title":"Biomaterials"},{"issue":"9","key":"2656_CR17","doi-asserted-by":"publisher","first-page":"2","DOI":"10.3390\/md1709049","volume":"17","author":"SAM Khalifa","year":"2019","unstructured":"Khalifa SAM, Elias N, Farag MA, Chen L, Saeed A, Hegazy MF, et al. Marine natural products: a source of novel anticancer drugs. Mar Drugs. 2019;17(9):2\u201331. https:\/\/doi.org\/10.3390\/md1709049.","journal-title":"Mar Drugs"},{"key":"2656_CR18","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/3432235","author":"S Bagheri-Josheghani","year":"2022","unstructured":"Bagheri-Josheghani S, Bakhshi B. Investigation of the antibacterial and antibiofilm activity of selenium nanoparticles against\u00a0vibrio cholerae\u00a0as a potent therapeutics. Can J Infect Dis Med Microbiol. 2022. https:\/\/doi.org\/10.1155\/2022\/3432235.","journal-title":"Can J Infect Dis Med Microbiol"},{"issue":"2","key":"2656_CR19","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1007\/s00203-020-02042-3","volume":"203","author":"HS Abbas","year":"2021","unstructured":"Abbas HS, Abou Baker DH, Ahmed EA. Cytotoxicity and antimicrobial efficiency of selenium nanoparticles biosynthesized by Spirulina platensis. Archiv Microbiol. 2021;203(2):523\u201332. https:\/\/doi.org\/10.1007\/s00203-020-02042-3.","journal-title":"Archiv Microbiol"},{"issue":"6","key":"2656_CR20","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.5530\/pj.2020.12.188","volume":"12","author":"T Francis","year":"2020","unstructured":"Francis T, Rajeshkumar S, Roy A, Lakshmi T. Anti-inflammatory and cytotoxic effect of arrowroot mediated selenium nanoparticles. Phcog J. 2020;12(6):1363\u20137. https:\/\/doi.org\/10.5530\/pj.2020.12.188.","journal-title":"Phcog J"},{"key":"2656_CR21","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.bmt.2023.12.001","volume":"6","author":"K Karthik","year":"2024","unstructured":"Karthik K, Cheriyan BV, Rajeshkumar S, Gopalakrishnan M. A review on selenium nanoparticles and their biomedical applications. Biomed Technol. 2024;6:61\u201374. https:\/\/doi.org\/10.1016\/j.bmt.2023.12.001.","journal-title":"Biomed Technol"},{"issue":"12","key":"2656_CR22","doi-asserted-by":"publisher","first-page":"4100","DOI":"10.3390\/ijms19124100","volume":"19","author":"M Ovais","year":"2018","unstructured":"Ovais M, Khalil AT, Ayaz M, Ahmad I, Nethi SK, Mukherjee S. Biosynthesis of metal nanoparticles via microbial enzymes: a mechanistic approach. Int J Mol Sci. 2018;19(12):4100. https:\/\/doi.org\/10.3390\/ijms19124100.","journal-title":"Int J Mol Sci"},{"key":"2656_CR23","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1007\/s11627-020-10150-4","volume":"57","author":"N Anwar","year":"2021","unstructured":"Anwar N, Wahid J, Uddin J, Khan A, Shah M, Shah SA, Subhan F, Khan MA, Ali K, Rauf M, Arif M. Phytosynthesis of poly (Ethylene Glycol) methacrylate-hybridized gold nanoparticles from C tuberculata: their structural characterization and potential for in vitro growth in Banana. Vitr Cell Dev Biol Plant. 2021;57:248\u201360. https:\/\/doi.org\/10.1007\/s11627-020-10150-4.","journal-title":"Vitr Cell Dev Biol Plant"},{"key":"2656_CR24","doi-asserted-by":"publisher","first-page":"7879403","DOI":"10.1155\/2018\/7879403","volume":"2018","author":"A Arya","year":"2018","unstructured":"Arya A, Gupta K, Chundawat TS, Vaya D. Biogenic synthesis of copper and silver nanoparticles using green Alga Botryococcus braunii and its antimicrobial activity. Bioinorg Chem Appl. 2018;2018:7879403. https:\/\/doi.org\/10.1155\/2018\/7879403.","journal-title":"Bioinorg Chem Appl"},{"key":"2656_CR25","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.matlet.2013.10.107","volume":"116","author":"B Sharma","year":"2014","unstructured":"Sharma B, Purkayastha D, Hazra S, Gogoi L, Bhattacharjee C, Ghosh N, Rout J. Biosynthesis of gold nanoparticles using a freshwater green alga. Prasiola Crispa Materials Lett. 2014;116:94\u20137. https:\/\/doi.org\/10.1016\/j.matlet.2013.10.107.","journal-title":"Prasiola Crispa Materials Lett"},{"issue":"2","key":"2656_CR26","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.jgeb.2016.09.00","volume":"14","author":"MM El-Sheekh","year":"2016","unstructured":"El-Sheekh MM, El-Kassas HY. Algal production of nano-silver and gold: their antimicrobial and cytotoxic activities: a review. J Genet Eng Biotechnol. 2016;14(2):299\u2013310. https:\/\/doi.org\/10.1016\/j.jgeb.2016.09.00.","journal-title":"J Genet Eng Biotechnol"},{"issue":"163","key":"2656_CR27","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2019.105656","volume":"1","author":"P Khanna","year":"2019","unstructured":"Khanna P, Kaur A, Goyal D. Algae-based metallic nanoparticles: synthesis, characterization and applications. J Microbiol Methods. 2019;1(163): 105656.","journal-title":"J Microbiol Methods"},{"issue":"3","key":"2656_CR28","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1080\/09603123.2020.1789946","volume":"32","author":"MM El-Sheekh","year":"2022","unstructured":"El-Sheekh MM, Shabaan MT, Hassan L, Morsi HH. Antiviral activity of algae biosynthesized silver and gold nanoparticles against Herpes simplex (HSV-1) virus in vitro using cell-line culture technique. Int J Environ Health Res. 2022;32(3):616\u201327. https:\/\/doi.org\/10.1080\/09603123.2020.1789946.","journal-title":"Int J Environ Health Res"},{"issue":"20","key":"2656_CR29","doi-asserted-by":"publisher","first-page":"8758","DOI":"10.3390\/su16208758","volume":"16","author":"A Sidorowicz","year":"2024","unstructured":"Sidorowicz A, Fais G, Desogus F, Loy F, Licheri R, Lai N, Cao G, Concas A. Eco-friendly photocatalytic treatment of dyes with Ag nanoparticles obtained through sustainable process involving Spirulina platensis. Sustainability. 2024;16(20):8758. https:\/\/doi.org\/10.3390\/su16208758.","journal-title":"Sustainability"},{"key":"2656_CR30","unstructured":"Zarrouk C. Contribution a l'etude d'une Cyanophycee. Influence de Divers Facteurs Physiques et Chimiques sur la croissance et la photosynthese de Spirulina mixima. Thesis. University of Paris, France.. 1966."},{"issue":"74","key":"2656_CR31","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1134\/S1061933X12060051","volume":"2012","author":"GA Dorofeev","year":"2012","unstructured":"Dorofeev GA, Streletskii AN, Povstugar IV, et al. Determination of nanoparticle sizes by X-ray diffraction. Colloid J. 2012;2012(74):675\u201385. https:\/\/doi.org\/10.1134\/S1061933X12060051.","journal-title":"Colloid J"},{"issue":"2","key":"2656_CR32","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/S0308-8146(00)00324-1","volume":"73","author":"MB Arnao","year":"2001","unstructured":"Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic contribution of total antioxidant activity. Food chem. 2001;73(2):239\u201344. https:\/\/doi.org\/10.1016\/S0308-8146(00)00324-1.","journal-title":"Food chem"},{"key":"2656_CR33","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1007\/s12247-023-09745-2","volume":"18","author":"NS Elkholy","year":"2023","unstructured":"Elkholy NS, Hariri MLM, Mohammed HS, et al. Lutein and \u03b2-carotene characterization in free and nanodispersion forms in terms of antioxidant activity and cytotoxicity. J Pharm Innov. 2023;18:1727\u201344. https:\/\/doi.org\/10.1007\/s12247-023-09745-2.","journal-title":"J Pharm Innov"},{"issue":"1","key":"2656_CR34","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.aca.2007.04.037","volume":"593","author":"C Samaniego S\u00e1nchez","year":"2007","unstructured":"Samaniego S\u00e1nchez C, Troncoso Gonz\u00e1lez AM, Garc\u00eda-Parrilla MC, Quesada Granados JJ, Garc\u00eda L, de la Serrana H, L\u00f3pez Mart\u00ednez MC. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Anal Chim Acta. 2007;593(1):103\u20137. https:\/\/doi.org\/10.1016\/j.aca.2007.04.037.","journal-title":"Anal Chim Acta"},{"issue":"4","key":"2656_CR35","doi-asserted-by":"publisher","first-page":"1154","DOI":"10.1016\/j.foodchem.2008.09.004","volume":"113","author":"LT Ling","year":"2009","unstructured":"Ling LT, Yap SA, Radhakrishnan AK, Subramaniam T, Cheng HM, Palanisamy UD. Standardised mangifera indica extract is an ideal antioxidant. Food Chem. 2009;113(4):1154\u20139. https:\/\/doi.org\/10.1016\/j.foodchem.2008.09.004.","journal-title":"Food Chem"},{"issue":"6","key":"2656_CR36","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1093\/carcin\/10.6.1003","volume":"10","author":"RJ Ruch","year":"1989","unstructured":"Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989;10(6):1003\u20138. https:\/\/doi.org\/10.1093\/carcin\/10.6.1003.","journal-title":"Carcinogenesis"},{"issue":"8","key":"2656_CR37","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1055\/s-2006-957563","volume":"64","author":"JN Eloff","year":"1998","unstructured":"Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998;64(8):711\u20133. https:\/\/doi.org\/10.1055\/s-2006-957563.","journal-title":"Planta Med"},{"key":"2656_CR38","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/420601","volume":"2013","author":"N Lall","year":"2013","unstructured":"Lall N, Henley-Smith CJ, De Canha MN, Oosthuizen CB, Berrington D. Viability reagent, prestoblue, in comparison with other available reagents, utilized in cytotoxicity and antimicrobial assays. Int J Microbiol. 2013;2013: 420601. https:\/\/doi.org\/10.1155\/2013\/420601.","journal-title":"Int J Microbiol"},{"key":"2656_CR39","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1007\/s40820-015-0040-x","volume":"7","author":"A Sirelkhatim","year":"2015","unstructured":"Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, Hasan H, Mohamad D. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-Micro Lett. 2015;7:219\u201342.","journal-title":"Nano-Micro Lett"},{"issue":"4","key":"2656_CR40","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1111\/j.1365-2958.2011.07867.x","volume":"82","author":"A Seper","year":"2011","unstructured":"Seper A, Fengler VH, Roier S, Wolinski H, Kohlwein SD, Bishop AL, Camilli A, Reidl J, Schild S. Extracellular nucleases and extracellular DNA play important roles in Vibrio cholerae biofilm formation. Mol Microbiol. 2011;82(4):1015\u201337. https:\/\/doi.org\/10.1111\/j.1365-2958.2011.07867.x.","journal-title":"Mol Microbiol"},{"key":"2656_CR41","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.10288","volume":"8","author":"R Elamary","year":"2020","unstructured":"Elamary R, Salem WM. Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria. Peer J. 2020;8: e10288. https:\/\/doi.org\/10.7717\/peerj.10288.","journal-title":"Peer J"},{"issue":"1","key":"2656_CR42","doi-asserted-by":"publisher","first-page":"7981","DOI":"10.1038\/s41598-021-87079-0","volume":"11","author":"AS Yassein","year":"2021","unstructured":"Yassein AS, Hassan MM, Elamary RB. Prevalence of lipase producer Aspergillus niger in nuts and anti-biofilm efficacy of its crude lipase against some human pathogenic bacteria. Sci Rep. 2021;11(1):7981. https:\/\/doi.org\/10.1038\/s41598-021-87079-0.","journal-title":"Sci Rep"},{"key":"2656_CR43","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1002\/(SICI)1099-1565(200005\/06)11:3","volume":"11","author":"F Hadacek","year":"2000","unstructured":"Hadacek F, Greger H. Testing of antifungal natural products: methodologies, comparability of results and assay choice. Phytochem Anal. 2000;11:137\u201347. https:\/\/doi.org\/10.1002\/(SICI)1099-1565(200005\/06)11:3.","journal-title":"Phytochem Anal"},{"key":"2656_CR44","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1186\/s13104-017-3001-2","volume":"10","author":"J Suurbaar","year":"2017","unstructured":"Suurbaar J, Mosobil R, Donor AM. Antibacterial and antifungal activities and phytochemical profile of leaf extract from different extractants of Ricinus communis against selected pathogens. BMC Res Notes. 2017;10:660. https:\/\/doi.org\/10.1186\/s13104-017-3001-2.","journal-title":"BMC Res Notes"},{"key":"2656_CR45","unstructured":"Clinical and Laboratory Standard Institute (2015) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard (10th ed.). CLSI document M07-A10. Wayne, PA: CLSI."},{"key":"2656_CR46","doi-asserted-by":"publisher","first-page":"1413","DOI":"10.3390\/app9071413","volume":"9","author":"M Bla\u017ei\u0107","year":"2019","unstructured":"Bla\u017ei\u0107 M, Bala\u017e AM, Prodanovi\u0107 O, Popovi\u0107 N, Ostafe R, Fischer R, Prodanovi\u0107 R. Directed evolution of cellobiose dehydrogenase on the surface of yeast cells using resazurin-based fluorescent assay. Appl Sci. 2019;9:1413. https:\/\/doi.org\/10.3390\/app9071413.","journal-title":"Appl Sci"},{"key":"2656_CR47","doi-asserted-by":"publisher","first-page":"012131","DOI":"10.1088\/1742-6596\/1299\/1\/012131","volume":"1299","author":"PA Akinduti","year":"2019","unstructured":"Akinduti PA, Motayo B, Idowu OM, Isibor PO, Olasehinde GI, Obafemi YD, Ugboko HU, Oyewale JO, Oluwadun A, Adeyemi GA. Suitability of spectrophotometric assay for determination of honey microbial inhibition. J Phys Conf Ser. 2019;1299:012131. https:\/\/doi.org\/10.1088\/1742-6596\/1299\/1\/012131.","journal-title":"J Phys Conf Ser"},{"issue":"8","key":"2656_CR48","doi-asserted-by":"publisher","first-page":"3105","DOI":"10.17485\/ijst\/2012\/v5i8.10","volume":"5","author":"C Dandjesso","year":"2012","unstructured":"Dandjesso C, Klotoa JR, Do\u03bcgnon TV, S\u00e8gbo J, At\u00e8gbo JM, Gbaguidi F, et al. Phytochemistry and hemostatic properties of some medicinal plants sold as anti-hemorrhagic in Cotonou markets (Benin). Ind J Sci Technol. 2012;5(8):3105\u20139. https:\/\/doi.org\/10.17485\/ijst\/2012\/v5i8.10.","journal-title":"Ind J Sci Technol"},{"issue":"46","key":"2656_CR49","doi-asserted-by":"publisher","first-page":"30998","DOI":"10.1021\/acsomega.1c03776","volume":"6","author":"N Shanthi","year":"2021","unstructured":"Shanthi N, Arumugam P, Murugan M, Sudhakar MP, Arunkumar K. Extraction of fucoidan from Turbinaria decurrens and the synthesis of fucoidan-coated AgNPs for anticoagulant application. ACS Omega. 2021;6(46):30998\u20131008. https:\/\/doi.org\/10.1021\/acsomega.1c03776.","journal-title":"ACS Omega"},{"issue":"13","key":"2656_CR50","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1093\/jnci\/82.13.1107","volume":"82","author":"P Skehan","year":"2019","unstructured":"Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 2019;82(13):1107\u201312. https:\/\/doi.org\/10.1093\/jnci\/82.13.1107.","journal-title":"J Natl Cancer Inst"},{"key":"2656_CR51","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.toxlet.2018.04.008","volume":"291","author":"RM Allam","year":"2018","unstructured":"Allam RM, Al-Abd AM, Khedr A, Sharaf OA, Nofal SM, Khalifa AE, Mosli HA, Abdel-Naim AB. Fingolimod interrupts the cross talk between estrogen metabolism and sphingolipid metabolism within prostate cancer cells. Toxicol Lett. 2018;291:77\u201385. https:\/\/doi.org\/10.1016\/j.toxlet.2018.04.008.","journal-title":"Toxicol Lett"},{"issue":"14","key":"2656_CR52","doi-asserted-by":"publisher","first-page":"4326","DOI":"10.3390\/molecules26144326","volume":"26","author":"C Kim","year":"2021","unstructured":"Kim C, Le D, Lee M. Diterpenoids isolated from podocarpus macrophyllus inhibited the inflammatory mediators in LPS-induced HT-29 and RAW 264.7 Cells. Molecules. 2021;26(14):4326. https:\/\/doi.org\/10.3390\/molecules26144326.","journal-title":"Molecules"},{"key":"2656_CR53","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.106171","volume":"129","author":"AHH Ahmed","year":"2022","unstructured":"Ahmed AHH, Mohamed MFA, Allam RM, Nafady A, Mohamed SK, Gouda AE, Beshr EAM. Design, synthesis, and molecular docking of novel pyrazole-chalcone analogs of lonazolac as 5-LOX, iNOS and tubulin polymerization inhibitors with potential anticancer and anti-inflammatory activities. Bioorg Chem. 2022;129: 106171. https:\/\/doi.org\/10.1016\/j.bioorg.2022.106171.","journal-title":"Bioorg Chem"},{"issue":"9","key":"2656_CR54","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.3390\/ph17091247","volume":"17","author":"L Rudi","year":"2024","unstructured":"Rudi L, Zinicovscaia I, Cepoi L, Chiriac T, Grozdov D, Kravtsova A. The impact of silver nanoparticles functionalized with spirulina protein extract on rats. Pharmaceuticals (Basel). 2024;17(9):1247. https:\/\/doi.org\/10.3390\/ph17091247.","journal-title":"Pharmaceuticals (Basel)"},{"issue":"1","key":"2656_CR55","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1515\/gps-2021-0005","volume":"10","author":"B ElSaied","year":"2021","unstructured":"ElSaied B, Diab A, Tayel A, Alghuthaymi M, Moussa S. Potent antibacterial action of phycosynthesized selenium nanoparticles using Spirulina platensis extract. Green Proc Synthesis. 2021;10(1):49\u201360. https:\/\/doi.org\/10.1515\/gps-2021-0005.","journal-title":"Green Proc Synthesis"},{"issue":"112","key":"2656_CR56","doi-asserted-by":"publisher","first-page":"92799","DOI":"10.1039\/C5RA17921A","volume":"5","author":"S Shirsat","year":"2015","unstructured":"Shirsat S, Kadam A, Naushad M, Mane RS. Selenium nanostructures: microbial synthesis and applications. RSC Adv. 2015;5(112):92799\u2013811. https:\/\/doi.org\/10.1039\/C5RA17921A.","journal-title":"RSC Adv"},{"key":"2656_CR57","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2024.101760","volume":"39","author":"S Vasanthakumar","year":"2024","unstructured":"Vasanthakumar S, Manikandan M, Arumugam M. Green synthesis, characterization and functional validation of bio-transformed selenium nanoparticles. Biochem Biophys Rep. 2024;39: 101760. https:\/\/doi.org\/10.1016\/j.bbrep.2024.101760.","journal-title":"Biochem Biophys Rep"},{"issue":"11","key":"2656_CR58","doi-asserted-by":"publisher","first-page":"8248","DOI":"10.1016\/j.arabjc.2020.01.009","volume":"13","author":"C Kishore","year":"2020","unstructured":"Kishore C, Dianxue C, Diaa F, Ahmer S, Abdul Qadeer D, Kai Z, Muhammad L, Ghazanfar M, Shu D. Green synthesis, characterization and photocatalytic application of silver nanoparticles synthesized by various plant extracts. Arab J Chem. 2020;13(11):8248\u201361. https:\/\/doi.org\/10.1016\/j.arabjc.2020.01.009.","journal-title":"Arab J Chem"},{"issue":"2","key":"2656_CR59","doi-asserted-by":"publisher","first-page":"773","DOI":"10.21608\/ejbo.2024.267306.2692","volume":"64","author":"AA Fath-Alla","year":"2024","unstructured":"Fath-Alla AA, Khalil NM, As M, Abd El-Ghany MN. Antiradical and anti-inflammatory activity of Saccharomyces cerevisiae mediated selenium nanoparticles. Egypt J Bot. 2024;64(2):773\u201387. https:\/\/doi.org\/10.21608\/ejbo.2024.267306.2692.","journal-title":"Egypt J Bot"},{"issue":"2","key":"2656_CR60","first-page":"385","volume":"6","author":"D Mubarak Ali","year":"2011","unstructured":"Mubarak Ali D, Sasikala M, Gunasekaran M, Thajuddin N. Biosynthesis and characterization of silver nanoparticles using marine cyanobacterium, Oscillatoria willei ntdm01. Dig J Nanomater and Biostructures. 2011;6(2):385\u201390.","journal-title":"Dig J Nanomater and Biostructures"},{"issue":"8","key":"2656_CR61","doi-asserted-by":"publisher","first-page":"4054","DOI":"10.52711\/0974-360X.2024.00629","volume":"17","author":"SY Alqaraleh","year":"2024","unstructured":"Alqaraleh SY, Al-Zereini WA, Mwafi NR, Jaffal SM, Al-Qtaitat AI. The green synthesis of selenium nanoparticles: a comprehensive review on methodology, characterization and biomedical applications. Res J Pharm Technol. 2024;17(8):4054\u201362. https:\/\/doi.org\/10.52711\/0974-360X.2024.00629.","journal-title":"Res J Pharm Technol"},{"issue":"38","key":"2656_CR62","doi-asserted-by":"publisher","first-page":"34725","DOI":"10.1021\/acsami.9b12319","volume":"11","author":"W Cong","year":"2019","unstructured":"Cong W, Bai R, Li YF, Wang L, Chen C. Selenium nanoparticles as an efficient nanomedicine for the therapy of Huntington\u2019s disease. ACS Appl Mater Interfaces. 2019;11(38):34725\u201335. https:\/\/doi.org\/10.1021\/acsami.9b12319.","journal-title":"ACS Appl Mater Interfaces"},{"key":"2656_CR63","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s40097-021-00435-4","volume":"28","author":"K Pyrzynska","year":"2021","unstructured":"Pyrzynska K, Sentkowska A. Biosynthesis of selenium nanoparticles using plant extracts. J Nanostructure Chem. 2021;28:1\u20134. https:\/\/doi.org\/10.1007\/s40097-021-00435-4.","journal-title":"J Nanostructure Chem"},{"issue":"2","key":"2656_CR64","doi-asserted-by":"publisher","first-page":"112","DOI":"10.33263\/BRIAC132.112","volume":"13","author":"SY Al-Qaraleh","year":"2023","unstructured":"Al-Qaraleh SY, Al-Zereini WA, Oran SA. Phyto-decoration of selenium nanoparticles using moringa peregrina (forssk) fiori aqueous extract: chemical characterization and bioactivity evaluation. Biointerface Res Appl Chem. 2023;13(2):112. https:\/\/doi.org\/10.33263\/BRIAC132.112.","journal-title":"Biointerface Res Appl Chem"},{"key":"2656_CR65","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.colcom.2018.12.004","volume":"29","author":"S Menon","year":"2019","unstructured":"Menon S, Agarwal H, Shanmugam VK. Efficacy of biogenic selenium nanoparticles from an extract of ginger towards evaluation on antimicrobial and antioxidant activities. Colloid Interf Sci Commun. 2019;29:1\u20138. https:\/\/doi.org\/10.1016\/j.colcom.2018.12.004.","journal-title":"Colloid Interf Sci Commun"},{"issue":"7","key":"2656_CR66","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1080\/21691401.2017.1373657","volume":"46","author":"W Zhang","year":"2018","unstructured":"Zhang W, Zhang J, Ding D, Zhang L, Muehlmann LA, Deng SE, Wang X, Li W, Zhang W. Synthesis and antioxidant properties of Lycium barbarum polysaccharides capped selenium nanoparticles using tea extract. Artif Cells Nanomed Biotechnol. 2018;46(7):1463\u201370. https:\/\/doi.org\/10.1080\/21691401.2017.1373657.","journal-title":"Artif Cells Nanomed Biotechnol"},{"key":"2656_CR67","doi-asserted-by":"publisher","DOI":"10.1016\/j.jallcom.2023.171235","volume":"965","author":"S Elnobi","year":"2023","unstructured":"Elnobi S, Dongol M, Soga T, Abuelwafa A. Charge transport, photoresponse and impedance Spectroscopy for Au\/NiTPP\/n-Si\/Al Diode. J Alloy Compd. 2023;965: 171235. https:\/\/doi.org\/10.1016\/j.jallcom.2023.171235.","journal-title":"J Alloy Compd"},{"issue":"11","key":"2656_CR68","doi-asserted-by":"publisher","first-page":"2269","DOI":"10.1021\/acs.bioconjchem.1c00402","volume":"32","author":"J Liu","year":"2021","unstructured":"Liu J, Ma L, Zhang G, Chen Y, Wang Z. Recent progress of surface modified nanomaterials for scavenging reactive oxygen species in organism. Bioconjug Chem. 2021;32(11):2269\u201389. https:\/\/doi.org\/10.1021\/acs.bioconjchem.1c00402.","journal-title":"Bioconjug Chem"},{"key":"2656_CR69","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.ijbiomac.2017.03.016","volume":"99","author":"Y Xiao","year":"2017","unstructured":"Xiao Y, Huang Q, Zheng Z, Guan H, Liu S. Construction of a Cordyceps sinensis exopolysaccharide-conjugated selenium nanoparticles and enhancement of their antioxidant activities. Int J Biol Macromol. 2017;99:483\u201391. https:\/\/doi.org\/10.1016\/j.ijbiomac.2017.03.016.","journal-title":"Int J Biol Macromol"},{"key":"2656_CR70","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemin.2022.100019","volume":"2","author":"N Jha","year":"2022","unstructured":"Jha N, Esakkiraj P, Annamalai A, Kant Lakra A, Naik S, Arul V. Synthesis, optimization, and physicochemical characterization of selenium nanoparticles from polysaccharide of mangrove Rhizophora mucronata with potential bioactivities. J Trace Elements Min (JTEMIN). 2022;2: 100019. https:\/\/doi.org\/10.1016\/j.jtemin.2022.100019.","journal-title":"J Trace Elements Min (JTEMIN)"},{"key":"2656_CR71","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s12649-023-02233-1","volume":"15","author":"JM Sonali","year":"2023","unstructured":"Sonali JM, Krishnaswamy VG, Rangasamy G, Kumar P, Rajagopal R. Efficacy of biogenic selenium nanoparticles from pseudomonas libanesis towards growth enhancement of Okra. Waste Biomass Valori. 2023;15:1\u201314. https:\/\/doi.org\/10.1007\/s12649-023-02233-1.","journal-title":"Waste Biomass Valori"},{"issue":"1","key":"2656_CR72","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1177\/08853282231182765","volume":"38","author":"M Zhu","year":"2023","unstructured":"Zhu M, Zhang Y, Zhang C, Chen L, Kuang Y. Rutin modified selenium nanoparticles reduces cell oxidative damage induced by H2O2 by activating Nrf2\/HO-1 signaling pathway. J Biomater Appl. 2023;38(1):109\u201321. https:\/\/doi.org\/10.1177\/08853282231182765.","journal-title":"J Biomater Appl"},{"key":"2656_CR73","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.685784","volume":"11","author":"Y An","year":"2021","unstructured":"An Y, Zhao J. Functionalized selenium nanotherapeutics synergizes with zoledronic acid to suppress prostate cancer cell growth through induction of mitochondria-mediated apoptosis and cell cycle s phase arrest. Front Oncol. 2021;11: 685784. https:\/\/doi.org\/10.3389\/fonc.2021.685784.","journal-title":"Front Oncol"},{"issue":"1","key":"2656_CR74","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1038\/s41598-020-80327-9","volume":"11","author":"V Cittrarasu","year":"2021","unstructured":"Cittrarasu V, Kaliannan D, Dharman K, Maluventhen V, Easwaran M, Liu WC, Balasubramanian B, Arumugam M. Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities. Sci Rep. 2021;11(1):1032. https:\/\/doi.org\/10.1038\/s41598-020-80327-9.","journal-title":"Sci Rep"},{"key":"2656_CR75","doi-asserted-by":"publisher","first-page":"3731","DOI":"10.2147\/IJN.S106289","volume":"11","author":"G Guisbiers","year":"2016","unstructured":"Guisbiers G, Wang Q, Khachatryan E, Mimun LC, Mendoza-Cruz R, Larese-Casanova P, Webster TJ, Nash KL. Inhibition of E. coli and S. aureus with selenium nanoparticles synthesized by pulsed laser ablation in deionized water. Int J Nanomed. 2016;11:3731\u20136. https:\/\/doi.org\/10.2147\/IJN.S106289.","journal-title":"Int J Nanomed"},{"key":"2656_CR76","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2020.110630","volume":"194","author":"H Zhang","year":"2021","unstructured":"Zhang H, Li Z, Dai C, Wang P, Fan S, Yu B, Qu Y. Antibacterial properties and mechanism of selenium nanoparticles synthesized by Providencia sp. DCX Environ Res. 2021;194: 110630. https:\/\/doi.org\/10.1016\/j.envres.2020.110630.","journal-title":"DCX Environ Res"},{"key":"2656_CR77","doi-asserted-by":"publisher","DOI":"10.26459\/hueuni-jard.v128i3b.4615","author":"N Van Khanh","year":"2019","unstructured":"Van Khanh N, Van Cu P. Antibacterial activity of silver nanoparticles against aeromonas spp. and Vibrio spp. isolated from aquaculture water environment in Thua Thien Hue. Hue University J Sci. 2019. https:\/\/doi.org\/10.26459\/hueuni-jard.v128i3b.4615.","journal-title":"Hue University J Sci"},{"key":"2656_CR78","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/j.jtemb.2014.07.020","volume":"29","author":"M Shakibaie","year":"2015","unstructured":"Shakibaie M, Forootanfar H, Golkari Y, Mohammadi-Khorsand T, Shakibaie MR. Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis. J Trace Elem Med Biol. 2015;29:235\u201341. https:\/\/doi.org\/10.1016\/j.jtemb.2014.07.020.","journal-title":"J Trace Elem Med Biol"},{"key":"2656_CR79","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/s12896-024-00855-4","volume":"24","author":"M Eydelkhani","year":"2024","unstructured":"Eydelkhani M, Kiabi S, Nowruzi B. In vitro assessment of the effect of magnetic fields on efficacy of biosynthesized selenium nanoparticles by Alborzia kermanshahica. BMC Biotechnol. 2024;24:27. https:\/\/doi.org\/10.1186\/s12896-024-00855-4.","journal-title":"BMC Biotechnol"},{"key":"2656_CR80","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtherbio.2022.103195","volume":"104","author":"AE Abdel-Moneim","year":"2022","unstructured":"Abdel-Moneim AE, Shehata AM, Selim DA, El-Saadony MT, Mesalam NM, Saleh AA. Spirulina platensis and biosynthesized selenium nanoparticles improve performance, antioxidant status, humoral immunity and dietary and ileal microbial populations of heat-stressed broilers. J Therm Biol. 2022;104: 103195. https:\/\/doi.org\/10.1016\/j.jtherbio.2022.103195.","journal-title":"J Therm Biol"},{"issue":"1","key":"2656_CR81","doi-asserted-by":"publisher","first-page":"47","DOI":"10.18502\/ajmb.v13i1.4578","volume":"13","author":"E Lotfali","year":"2021","unstructured":"Lotfali E, Toreyhi H, Makhdoomi Sharabiani K, Fattahi A, Soheili A, Ghasemi R, Keymaram M, Rezaee Y, Iranpanah S. Comparison of antifungal properties of gold, silver, and selenium nanoparticles against amphotericin B-resistant\u00a0Candida glabrata\u00a0clinical isolates. Avicenna J Med Biotechnol. 2021;13(1):47\u201350. https:\/\/doi.org\/10.18502\/ajmb.v13i1.4578.","journal-title":"Avicenna J Med Biotechnol."},{"key":"2656_CR82","doi-asserted-by":"publisher","first-page":"11834","DOI":"10.1038\/s41598-022-15903-2","volume":"12","author":"A Fouda","year":"2022","unstructured":"Fouda A, Hassan SED, Eid AM, Abdel-Rahman MA, Hamza MF. Light enhanced the antimicrobial, anticancer, and catalytic activities of selenium nanoparticles fabricated by endophytic fungal strain, Penicillium crustosum EP-1. Sci Rep. 2022;12:11834. https:\/\/doi.org\/10.1038\/s41598-022-15903-2.","journal-title":"Sci Rep"},{"issue":"2","key":"2656_CR83","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0274679","volume":"18","author":"M Shahbaz","year":"2023","unstructured":"Shahbaz M, Akram A, Raja NI, Mukhtar T, Mehak A, Fatima N, Ajmal M, Ali K, Mustafa N, Abasi F. Antifungal activity of green synthesized selenium nanoparticles and their effect on physiological, biochemical, and antioxidant defense system of mango under mango malformation disease. PLoS ONE. 2023;18(2): e0274679. https:\/\/doi.org\/10.1371\/journal.pone.0274679.","journal-title":"PLoS ONE"},{"issue":"1","key":"2656_CR84","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1186\/s12896-023-00807-4","volume":"23","author":"B Nowruzi","year":"2023","unstructured":"Nowruzi B, Jalil BS, Metcalf JS. Antifungal screening of selenium nanoparticles biosynthesized by microcystin-producing Desmonostoc alborizicum. BMC Biotechnol. 2023;23(1):41. https:\/\/doi.org\/10.1186\/s12896-023-00807-4.","journal-title":"BMC Biotechnol"},{"issue":"22","key":"2656_CR85","doi-asserted-by":"publisher","first-page":"6983","DOI":"10.3390\/ma14226983","volume":"14","author":"AM Abdo","year":"2021","unstructured":"Abdo AM, Fouda A, Eid AM, Fahmy NM, Elsayed AM, Khalil AMA, Alzahrani OM, Ahmed AF, Soliman AM. Green synthesis of zinc oxide nanoparticles (ZnO-NPs) by Pseudomonas aeruginosa and their activity against pathogenic microbes and common house mosquito, Culex pipiens. Materials (Basel). 2021;14(22):6983. https:\/\/doi.org\/10.3390\/ma14226983.","journal-title":"Materials (Basel)"},{"issue":"2","key":"2656_CR86","doi-asserted-by":"publisher","DOI":"10.33448\/rsd-v11i2.25439","volume":"11","author":"VMAM Miranda","year":"2022","unstructured":"Miranda VMAM, Barbosa Filho JPM, Costa RMPB, Leite ACL, Oliveiravde M, Batista S, Pastrana L, Nascimento TP, Porto ALF. Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease. Res Soc Dev. 2022;11(2): e15311225439. https:\/\/doi.org\/10.33448\/rsd-v11i2.25439.","journal-title":"Res Soc Dev"},{"issue":"3","key":"2656_CR87","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1111\/trf.16259","volume":"61","author":"RA Henderson","year":"2021","unstructured":"Henderson RA, Judd M, Strauss ER, Gammie JS, Mazzeffi MA, Taylor BS, Tanaka KA. Hematologic evaluation of intraoperative autologous blood collection and allogeneic transfusion in cardiac surgery. Transfusion. 2021;61(3):788\u201398. https:\/\/doi.org\/10.1111\/trf.16259.","journal-title":"Transfusion"},{"issue":"8","key":"2656_CR88","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.680397","volume":"28","author":"F Altaf","year":"2021","unstructured":"Altaf F, Wu S, Kasim V. Role of fibrinolytic enzymes in anti-thrombosis therapy. Front Mol Biosci. 2021;28(8): 680397. https:\/\/doi.org\/10.3389\/fmolb.2021.680397.","journal-title":"Front Mol Biosci"},{"issue":"7","key":"2656_CR89","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1080\/10826068.2018.1479864","volume":"48","author":"A Lateef","year":"2018","unstructured":"Lateef A, Folarin BI, Oladejo SM, Akinola PO, Beukes LS, Gueguim-Kana EB. Characterization, antimicrobial, antioxidant, and anticoagulant activities of silver nanoparticles synthesized from Petiveria alliacea L. leaf extract. Prep Biochem Biotechnol. 2018;48(7):646\u201352. https:\/\/doi.org\/10.1080\/10826068.2018.1479864.","journal-title":"Prep Biochem Biotechnol"},{"key":"2656_CR90","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1016\/j.biopha.2016.09.057","volume":"84","author":"NZ Jin","year":"2016","unstructured":"Jin NZ, Gopinath SCB. Potential blood clotting factors and anticoagulants. Biomed Pharmacother. 2016;84:356\u201365. https:\/\/doi.org\/10.1016\/j.biopha.2016.09.057.","journal-title":"Biomed Pharmacother"},{"issue":"1","key":"2656_CR91","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1080\/10717544.2019.1606363","volume":"26","author":"L Li","year":"2019","unstructured":"Li L, He S, Yu L, Elshazly EH, Wang H, Chen K, Zhang S, Ke L, Gong R. Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells. Drug Deliv. 2019;26(1):499\u2013508. https:\/\/doi.org\/10.1080\/10717544.2019.1606363.","journal-title":"Drug Deliv"},{"issue":"4","key":"2656_CR92","doi-asserted-by":"publisher","first-page":"801","DOI":"10.3390\/molecules29040801","volume":"29","author":"S Sampath","year":"2024","unstructured":"Sampath S, Sunderam V, Manjusha M, Dlamini Z, Lawrance AV. Selenium nanoparticles: a comprehensive examination of synthesis techniques and their diverse applications in medical research and toxicology studies. Molecules. 2024;29(4):801. https:\/\/doi.org\/10.3390\/molecules29040801.","journal-title":"Molecules"},{"key":"2656_CR93","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2023.127357","volume":"82","author":"E Khaledizade","year":"2024","unstructured":"Khaledizade E, Tafvizi F, Jafari P. Anti-breast cancer activity of biosynthesized selenium nanoparticles using Bacillus coagulans supernatant. J Trace Elem Med Biol. 2024;82: 127357. https:\/\/doi.org\/10.1016\/j.jtemb.2023.127357.","journal-title":"J Trace Elem Med Biol"},{"key":"2656_CR94","doi-asserted-by":"publisher","first-page":"835","DOI":"10.2147\/IJN.S28278","volume":"7","author":"F Yang","year":"2012","unstructured":"Yang F, Tang Q, Zhong X, Bai Y, Chen T, Zhang Y, Li Y, Zheng W. Surface decoration by Spirulina polysaccharide enhances the cellular uptake and anticancer efficacy of selenium nanoparticles. Int J Nanomedicine. 2012;7:835\u201344. https:\/\/doi.org\/10.2147\/IJN.S28278.","journal-title":"Int J Nanomedicine"},{"key":"2656_CR95","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2020.102258","volume":"29","author":"B Toubhans","year":"2020","unstructured":"Toubhans B, Gazze SA, Bissardon C, Bohic S, Gourlan AT, Gonzalez D, Charlet L, Conlan RS, Francis LW. Selenium nanoparticles trigger alterations in ovarian cancer cell biomechanics. Nanomedicine. 2020;29: 102258. https:\/\/doi.org\/10.1016\/j.nano.2020.102258.","journal-title":"Nanomedicine"},{"key":"2656_CR96","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-024-09598-z","author":"JA Ansari","year":"2024","unstructured":"Ansari JA, Malik JA, Ahmed S, et al. Recent advances in the therapeutic applications of selenium nanoparticles. Mol Biol Rep. 2024. https:\/\/doi.org\/10.1007\/s11033-024-09598-z.","journal-title":"Mol Biol Rep"}],"container-title":["Microbial Cell Factories"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-025-02656-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12934-025-02656-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-025-02656-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T17:24:34Z","timestamp":1739553874000},"score":1,"resource":{"primary":{"URL":"https:\/\/microbialcellfactories.biomedcentral.com\/articles\/10.1186\/s12934-025-02656-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,7]]},"references-count":96,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["2656"],"URL":"https:\/\/doi.org\/10.1186\/s12934-025-02656-6","relation":{},"ISSN":["1475-2859"],"issn-type":[{"value":"1475-2859","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,7]]},"assertion":[{"value":"14 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 February 2025","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Update","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Funding statement has been updated.","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical Approval was granted (001 \/11\/ 2024) by the Faculty of Science, South Valley University, Egypt Research Ethics Committee (REC-FScSVU).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"39"}}