{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T15:57:46Z","timestamp":1764172666867,"version":"3.38.0"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773613"],"award-info":[{"award-number":["81773613"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2023-MS-199"],"award-info":[{"award-number":["2023-MS-199"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013099","name":"Scientific Research Fund of Liaoning Provincial Education Department","doi-asserted-by":"publisher","award":["JYTZD2023135"],"award-info":[{"award-number":["JYTZD2023135"]}],"id":[{"id":"10.13039\/501100013099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"DOI":"10.1186\/s12934-025-02678-0","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T12:56:45Z","timestamp":1740747405000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Improving activity of GenB3 and GenB4 in gentamicin dideoxygenation biosynthesis by semi-rational engineering"],"prefix":"10.1186","volume":"24","author":[{"given":"Hang","family":"Zhai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhijun","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiye","family":"Pei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Botong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianpu","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanzhang","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"2678_CR1","first-page":"3","volume":"2005","author":"A Schatz","year":"1944","unstructured":"Schatz A, Bugie E, Waksman SA. Streptomycin, a substance exhibiting antibiotic activity against gram-positive and gram-negative bacteria. Clin Orthop Relat Res. 1944;2005:3\u20136.","journal-title":"Clin Orthop Relat Res"},{"key":"2678_CR2","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1093\/cid\/ciz640","volume":"70","author":"LD Saravolatz","year":"2020","unstructured":"Saravolatz LD, Stein GE. Plazomicin: A new aminoglycoside. Clin Infect Dis. 2020;70:704\u20139.","journal-title":"Clin Infect Dis"},{"key":"2678_CR3","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1080\/15476286.2017.1285480","volume":"14","author":"L Bidou","year":"2017","unstructured":"Bidou L, Bugaud O, Belakhov V, Baasov T, Namy O. Characterization of new-generation aminoglycoside promoting premature termination codon readthrough in cancer cells. RNA Biol. 2017;14:378\u201388.","journal-title":"RNA Biol"},{"key":"2678_CR4","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1021\/acsomega.2c06112","volume":"8","author":"Z Izadi","year":"2023","unstructured":"Izadi Z, Barzegari E, Iranpanah A, Sajadimajd S, Derakhshankhah H. Gentamycin rationally repositioned to inhibit miR-34a ameliorates oxidative injury to PC12 cells. ACS OMEGA. 2023;8:771\u201381.","journal-title":"ACS OMEGA"},{"key":"2678_CR5","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1021\/cb4007387","volume":"9","author":"JL Childs-Disney","year":"2014","unstructured":"Childs-Disney JL, Yildirim I, Park H, Lohman JR, Guan L, Tran T, Sarkar P, Schatz GC, Disney MD. Structure of the myotonic dystrophy type 2 RNA and designed small molecules that reduce toxicity. ACS Chem Biol. 2014;9:538\u201350.","journal-title":"ACS Chem Biol"},{"key":"2678_CR6","doi-asserted-by":"publisher","first-page":"10956","DOI":"10.1002\/anie.201406465","volume":"53","author":"SG Rzuczek","year":"2014","unstructured":"Rzuczek SG, Park H, Disney MD. A toxic RNA catalyzes the in cellulo synthesis of its own inhibitor. Angew Chem Int Ed. 2014;53:10956\u20139.","journal-title":"Angew Chem Int Ed"},{"key":"2678_CR7","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1016\/j.omtn.2019.04.023","volume":"16","author":"M Wang","year":"2019","unstructured":"Wang M, Wu B, Shah SN, Lu P, Lu Q. Aminoglycoside enhances the delivery of antisense morpholino oligonucleotides in vitro and in Mdx mice. Mol Therapy - Nucleic Acids. 2019;16:663\u201374.","journal-title":"Mol Therapy - Nucleic Acids"},{"key":"2678_CR8","doi-asserted-by":"publisher","first-page":"3850","DOI":"10.1021\/acs.orglett.0c01107","volume":"22","author":"P Rajasekaran","year":"2020","unstructured":"Rajasekaran P, Crich D. Synthesis of gentamicin minor components: gentamicin B1 and gentamicin X2. Org Lett. 2020;22:3850\u20134.","journal-title":"Org Lett"},{"key":"2678_CR9","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1021\/ol802421d","volume":"11","author":"S Hanessian","year":"2009","unstructured":"Hanessian S, Szychowski J, Maianti JP. Synthesis and comparative antibacterial activity of verdamicin C2 and C2a. A new oxidation of primary allylic Azides in dihydro[2H]pyrans. Org Lett. 2009;11:429\u201332.","journal-title":"Org Lett"},{"key":"2678_CR10","doi-asserted-by":"publisher","first-page":"8564","DOI":"10.1021\/acs.orglett.2c03616","volume":"24","author":"S Jana","year":"2022","unstructured":"Jana S, Crich D. Synthesis of gentamicin minor components: gentamicin C1a and gentamicin C2b. Org Lett. 2022;24:8564\u20137.","journal-title":"Org Lett"},{"key":"2678_CR11","doi-asserted-by":"publisher","first-page":"1622","DOI":"10.1021\/acsinfecdis.3c00233","volume":"9","author":"S Jana","year":"2023","unstructured":"Jana S, Rajasekaran P, Haldimann K, Vasella A, B\u00f6ttger EC, Hobbie SN, Crich D. Synthesis of gentamicins C1, C2, and C2a and antiribosomal and antibacterial activity of gentamicins B1, C1, C1a, C2, C2a, C2b, and X2. ACS Infect Dis. 2023;9:1622\u201333.","journal-title":"ACS Infect Dis"},{"key":"2678_CR12","doi-asserted-by":"publisher","first-page":"e202303210","DOI":"10.1002\/slct.202303210","volume":"9","author":"J Xu","year":"2024","unstructured":"Xu J, Zhang X, Xu G, Zhang Y, Li H, Shi J. Design, synthesis and activity evaluation against Drug-Resistant Bacteria of 6\u2019-Amidine modified aminoglycoside derivatives. ChemistrySelect. 2024;9:e202303210.","journal-title":"ChemistrySelect"},{"key":"2678_CR13","doi-asserted-by":"publisher","first-page":"725916","DOI":"10.3389\/fmicb.2021.725916","volume":"12","author":"YH Ban","year":"2021","unstructured":"Ban YH, Song MC, Jeong JH, Kwun MS, Kim CR, Ryu HS, Kim E, Park JW, Lee DG, Yoon YJ. Microbial enzymatic synthesis of Amikacin analogs with antibacterial activity against Multidrug-Resistant pathogens. Front Microbiol. 2021;12:725916.","journal-title":"Front Microbiol"},{"key":"2678_CR14","doi-asserted-by":"publisher","first-page":"e202200497","DOI":"10.1002\/cmdc.202200497","volume":"18","author":"NJ Lee","year":"2023","unstructured":"Lee NJ, Kang W, Kwon Y, Oh JW, Jung H, Seo M, Seol Y, Wi JB, Ban YH, Yoon YJ, Park JW. Chemo-enzymatic synthesis of Pseudo-trisaccharide aminoglycoside antibiotics with enhanced nonsense Read-through inducer activity. ChemMedChem. 2023;18:e202200497.","journal-title":"ChemMedChem"},{"key":"2678_CR15","doi-asserted-by":"publisher","first-page":"9542","DOI":"10.1039\/D2GC03600B","volume":"24","author":"G Stojanovski","year":"2022","unstructured":"Stojanovski G, Hailes HC, Ward JM. Facile and selective N-alkylation of gentamicin antibiotics via chemoenzymatic synthesis. Green Chem. 2022;24:9542\u201351.","journal-title":"Green Chem"},{"key":"2678_CR16","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1186\/s12934-020-01317-0","volume":"19","author":"X Chen","year":"2020","unstructured":"Chen X, Zhang H, Zhou S, Bi M, Qi S, Gao H, Ni X, Xia H. The bifunctional enzyme, GenB4, catalyzes the last step of gentamicin 3\u2019,4\u2019-di-deoxygenation via reduction and transamination activities. Microb Cell Fact. 2020;19:62.","journal-title":"Microb Cell Fact"},{"key":"2678_CR17","doi-asserted-by":"publisher","first-page":"12274","DOI":"10.1021\/acscatal.1c03508","volume":"11","author":"SC Li","year":"2021","unstructured":"Li SC, Bury PD, Huang FL, Guo JH, Sun G, Reva A, Huang C, Jian XY, Li Y, Zhou JH, et al. Mechanistic insights into dideoxygenation in gentamicin biosynthesis. ACS Catal. 2021;11:12274\u201383.","journal-title":"ACS Catal"},{"key":"2678_CR18","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s12934-021-01558-7","volume":"20","author":"S Zhou","year":"2021","unstructured":"Zhou S, Chen X, Ni X, Liu Y, Zhang H, Dong M, Xia H. Pyridoxal-5\u2019-phosphate-dependent enzyme GenB3 catalyzes C-3\u2019,4\u2019-dideoxygenation in gentamicin biosynthesis. Microb Cell Fact. 2021;20:65.","journal-title":"Microb Cell Fact"},{"key":"2678_CR19","doi-asserted-by":"publisher","first-page":"114380","DOI":"10.1016\/j.mcat.2024.114380","volume":"565","author":"Z Kong","year":"2024","unstructured":"Kong Z, Tian T, Wang R, Xia H, Sun J, Zhai H, Ni X. Enhanced activity of C-3\u2019 and C-4\u2019 dideoxygenation of the aminoglycoside GenB3 by a semi-rational design strategy. Mol Catal. 2024;565:114380.","journal-title":"Mol Catal"},{"key":"2678_CR20","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.ab.2003.12.024","volume":"327","author":"JD Gawronski","year":"2004","unstructured":"Gawronski JD, Benson DR. Microtiter assay for glutamine synthetase biosynthetic activity using inorganic phosphate detection. Anal Biochem. 2004;327:114\u20138.","journal-title":"Anal Biochem"},{"key":"2678_CR21","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.1002\/cbic.201500258","volume":"16","author":"Y Gu","year":"2015","unstructured":"Gu Y, Ni X, Ren J, Gao H, Wang D, Xia H. Biosynthesis of epimers C2 and C2a in the gentamicin C complex. ChemBioChem. 2015;16:1933\u201342.","journal-title":"ChemBioChem"}],"container-title":["Microbial Cell Factories"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-025-02678-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12934-025-02678-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12934-025-02678-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T12:56:48Z","timestamp":1740747408000},"score":1,"resource":{"primary":{"URL":"https:\/\/microbialcellfactories.biomedcentral.com\/articles\/10.1186\/s12934-025-02678-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":21,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["2678"],"URL":"https:\/\/doi.org\/10.1186\/s12934-025-02678-0","relation":{},"ISSN":["1475-2859"],"issn-type":[{"value":"1475-2859","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]},"assertion":[{"value":"17 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 February 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"49"}}