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Despite producing polyols, such as erythritol, arabitol and mannitol, the re-utilization of mannitol is not as efficient as erythritol utilization. Genes involved in mannitol uptake and metabolism in <jats:italic>Y. lipolytica<\/jats:italic> remain undescribed. However, deletion of the <jats:italic>EYD1<\/jats:italic> gene (<jats:italic>YALI0F01650g<\/jats:italic>), believed to encode erythritol dehydrogenase, has been found to result in a high rate of growth on media containing mannitol as the sole carbon source. Therefore this unique feature was used for further fermentation studies on media containing macroalgal mannitol extracts, obtained from the brown alga <jats:italic>Fucus vesiculosus<\/jats:italic>, to produce value-added products.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The obtained strain <jats:italic>AJD \u0394eyd1Dga1<\/jats:italic> was able to uptake pure and algal mannitol efficiently and produce high amounts of lipids, thanks to overexpression of the <jats:italic>DGA1<\/jats:italic> gene (<jats:italic>YALI0E32769g<\/jats:italic>), encoding diacylglycerol (DAG) acyltransferase. The lipid content reached almost 32% of the overall dry biomass as compared to the wild type strain, where this value was more than 4 times lower. Additionally, the biomass at the end of the experiment was the highest among all of the tested strains, reaching 12.67\u00a0g\/L, more than 50% higher than the control strain.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The results of this study shed new light on the potential for the yeast <jats:italic>Y. lipolytica<\/jats:italic> to utilize macroalgae biomass as a carbon source for production of value-added products, including biomass and lipids. Moreover, the increased mannitol utilization capabilities can provide new insight into mannitol metabolism, including its uptake, which is especially crucial, as the metabolic pathways for all polyols produced by this organism seem to be closely intertwined.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12934-025-02699-9","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T03:29:25Z","timestamp":1742959765000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Engineered yeast Yarrowia lipolytica as a chassis for biosynthesis of fatty acids from mannitol and macroalgal biomass extracts"],"prefix":"10.1186","volume":"24","author":[{"given":"Mateusz","family":"Szczepa\u0144czyk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota A.","family":"Rzechonek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Dobrowolski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1604-1635","authenticated-orcid":false,"given":"Aleksandra M.","family":"Miro\u0144czuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"2699_CR1","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.ref.2018.12.006","volume":"28","author":"HA Alalwan","year":"2019","unstructured":"Alalwan HA, Alminshid AH, Aljaafari HAS. 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