{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T04:23:20Z","timestamp":1784175800966,"version":"3.55.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Health Science and Technology Project of Yunnan, China","award":["2018NS0156"],"award-info":[{"award-number":["2018NS0156"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cancer Cell Int"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Vestibular schwannoma (VS) is a kind of benign tumor deriving from the acoustic nerve sheath. Substantial long non-coding RNAs (lncRNAs) were illustrated to have crucial roles in multiple cancers. However, few lncRNAs were elucidated in VS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HCG11, miR-620 and ELK4 expression were tested by RT-qPCR. Gain-of-function experiments were conducted to confirm the effect of HCG11 on VS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HCG11 possessed a low expression in VS cell lines. Overexpression of HCG11 repressed cell proliferation but accelerated apoptosis of VS cells. Moreover, we identified ELK4 stimulated the transcription of HCG11 and their affinity was verified by ChIP assays. MiR-620 was chosen to be a target of HCG11 and it was tested to have a high expression in VS cell lines. Moreover, depletion of miR-620 could inhibit cell proliferative ability while fostering apoptosis rate of VS cells. ELK4 was low expressed in VS cell lines and knockdown of ELK4 could rescue the effects made by HCG11 overexpression on progression of VS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HCG11 could inhibit the growth of VS by targeting miR-620\/ELK4 in VS cells. HCG11 was a novel therapeutic target for VS treatment.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12935-020-01691-0","type":"journal-article","created":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T17:04:27Z","timestamp":1609866267000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["HCG11 up-regulation induced by ELK4 suppressed proliferation in vestibular schwannoma by targeting miR-620\/ELK4"],"prefix":"10.1186","volume":"21","author":[{"given":"Ruiqing","family":"Long","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuohui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3835-634X","authenticated-orcid":false,"given":"Hualin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"issue":"1","key":"1691_CR1","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/S1042-3680(18)30833-7","volume":"1","author":"RK Jackler","year":"1990","unstructured":"Jackler RK, Pitts LH. Acoustic neuroma. Neurosurg Clin N Am. 1990;1(1):199\u2013223.","journal-title":"Neurosurg Clin N Am"},{"issue":"6","key":"1691_CR2","first-page":"E1","volume":"60","author":"R McDonald","year":"2011","unstructured":"McDonald R. Acoustic neuroma: what the evidence says about evaluation and treatment. J Fam Pract. 2011;60(6):E1\u20134.","journal-title":"J Fam Pract"},{"key":"1691_CR3","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.cca.2018.07.004","volume":"485","author":"J Wang","year":"2018","unstructured":"Wang J, Su Z, Lu S, Fu W, Liu Z, Jiang X, Tai S. LncRNA HOXA-AS2 and its molecular mechanisms in human cancer. Clin Chim Acta. 2018;485:229\u201333.","journal-title":"Clin Chim Acta"},{"issue":"6","key":"1691_CR4","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/j.prp.2018.04.003","volume":"214","author":"KZ Thin","year":"2018","unstructured":"Thin KZ, Liu X, Feng X, Raveendran S, Tu JC. LncRNA-DANCR: a valuable cancer related long non-coding RNA for human cancers. Pathol Res Pract. 2018;214(6):801\u20135.","journal-title":"Pathol Res Pract"},{"issue":"4","key":"1691_CR5","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1016\/j.juro.2016.05.107","volume":"196","author":"S Zhang","year":"2016","unstructured":"Zhang S, Zhong G, He W, Yu H, Huang J, Lin T. lncRNA up-regulated in nonmuscle invasive bladder cancer facilitates tumor growth and acts as a negative prognostic factor of recurrence. J Urol. 2016;196(4):1270\u20138.","journal-title":"J Urol"},{"issue":"20","key":"1691_CR6","first-page":"4529","volume":"21","author":"HW Huang","year":"2017","unstructured":"Huang HW, Xie H, Ma X, Zhao F, Gao Y. Upregulation of LncRNA PANDAR predicts poor prognosis and promotes cell proliferation in cervical cancer. Eur Rev Med Pharmacol Sci. 2017;21(20):4529\u201335.","journal-title":"Eur Rev Med Pharmacol Sci"},{"issue":"3","key":"1691_CR7","doi-asserted-by":"publisher","first-page":"791","DOI":"10.3892\/ijo.2017.4066","volume":"51","author":"Y Xu","year":"2017","unstructured":"Xu Y, Zheng Y, Liu H, Li T. Modulation of IGF2BP1 by long non-coding RNA HCG11 suppresses apoptosis of hepatocellular carcinoma cells via MAPK signaling transduction. Int J Oncol. 2017;51(3):791\u2013800.","journal-title":"Int J Oncol"},{"issue":"2","key":"1691_CR8","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1093\/nar\/gkx1224","volume":"46","author":"L Shen","year":"2018","unstructured":"Shen L, Wang Q, Liu R, Chen Z, Zhang X, Zhou P, Wang Z. LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA. Nucleic Acids Res. 2018;46(2):717\u201329.","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"1691_CR9","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1089\/cbr.2018.2565","volume":"34","author":"X Zheng","year":"2019","unstructured":"Zheng X, Liu M, Song Y, Feng C. Long noncoding RNA-ATB impairs the function of tumor suppressor miR-126-mediated signals in endometrial cancer for tumor growth and metastasis. Cancer Biother Radiopharm. 2019;34(1):47\u201355.","journal-title":"Cancer Biother Radiopharm"},{"key":"1691_CR10","doi-asserted-by":"crossref","first-page":"e12615","DOI":"10.1111\/cpr.12615","volume":"52","author":"Y Chen","year":"2019","unstructured":"Chen Y, Bao C, Zhang X, Lin X, Huang H, Wang Z. Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496\/CPEB3 axis. Cell Prolif. 2019;52:e12615.","journal-title":"Cell Prolif"},{"issue":"1","key":"1691_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3233\/CBM-160240","volume":"19","author":"LL Mei","year":"2017","unstructured":"Mei LL, Qiu YT, Zhang B, Shi ZZ. MicroRNAs in esophageal squamous cell carcinoma: potential biomarkers and therapeutic targets. Cancer Biomarkers. 2017;19(1):1\u20139.","journal-title":"Cancer Biomarkers"},{"key":"1691_CR12","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.biopha.2015.10.005","volume":"76","author":"Y Mao","year":"2015","unstructured":"Mao Y, Liu J, Zhang D, Li B. MiR-1290 promotes cancer progression by targeting nuclear factor I\/X(NFIX) in esophageal squamous cell carcinoma (ESCC). Biomed Pharmacother. 2015;76:82\u201393.","journal-title":"Biomed Pharmacother"},{"issue":"31","key":"1691_CR13","doi-asserted-by":"publisher","first-page":"49217","DOI":"10.18632\/oncotarget.10247","volume":"7","author":"B de Melo Maia","year":"2016","unstructured":"de Melo Maia B, Rodrigues IS, Akagi EM, Soares do Amaral N, Ling H, Monroig P, Soares FA, Calin GA, Rocha RM. MiR-223-5p works as an oncomiR in vulvar carcinoma by TP63 suppression. Oncotarget. 2016;7(31):49217\u201331.","journal-title":"Oncotarget"},{"key":"1691_CR14","doi-asserted-by":"crossref","unstructured":"Li SL, Ma XH, Ji JF, Li H, Liu W, Lu FZ, Wu ST, Zhang Y: miR-1 association with cell proliferation inhibition and apoptosis in vestibular schwannoma by targeting VEGFA. Genet Mol Res. 2016, 15(4).","DOI":"10.4238\/gmr15048923"},{"issue":"9","key":"1691_CR15","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1097\/MAO.0b013e3181f20655","volume":"31","author":"JA Cioffi","year":"2010","unstructured":"Cioffi JA, Yue WY, Mendolia-Loffredo S, Hansen KR, Wackym PA, Hansen MR. MicroRNA-21 overexpression contributes to vestibular schwannoma cell proliferation and survival. Otol Neurotol. 2010;31(9):1455\u201362.","journal-title":"Otol Neurotol."},{"issue":"24","key":"1691_CR16","first-page":"8589","volume":"22","author":"JDC Tian","year":"2018","unstructured":"Tian JDC, Liang L. Involvement of circular RNA SMARCA5\/microRNA-620 axis in the regulation of cervical cancer cell proliferation, invasion and migration. European review for medical and pharmacological sciences. 2018;22(24):8589\u201398.","journal-title":"European review for medical and pharmacological sciences"},{"issue":"6","key":"1691_CR17","first-page":"5545","volume":"17","author":"H Wang","year":"2019","unstructured":"Wang H, Zhang H, Zeng J, Tan Y. ceRNA network analysis reveals prognostic markers for glioblastoma. Oncol Lett. 2019;17(6):5545\u201357.","journal-title":"Oncol Lett"},{"issue":"5","key":"1691_CR18","doi-asserted-by":"publisher","first-page":"337","DOI":"10.4132\/jptm.2016.05.20","volume":"50","author":"HS Lee","year":"2016","unstructured":"Lee HS, Jung W, Lee E, Chang H, Choi JH, Kim HG, Kim A, Kim BH. SIRT7, H3K18ac, and ELK4 immunohistochemical expression in hepatocellular carcinoma. J Pathol Transl Med. 2016;50(5):337\u201344.","journal-title":"J Pathol Transl Med"},{"key":"1691_CR19","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1186\/s12935-019-0927-6","volume":"19","author":"F Ding","year":"2019","unstructured":"Ding F, Lai J, Gao Y, Wang G, Shang J, Zhang D, Zheng S. NEAT1\/miR-23a-3p\/KLF3: a novel regulatory axis in melanoma cancer progression. Cancer Cell Int. 2019;19:217.","journal-title":"Cancer Cell Int"},{"key":"1691_CR20","doi-asserted-by":"publisher","first-page":"1540","DOI":"10.1016\/j.biopha.2018.08.105","volume":"107","author":"D Cheng","year":"2018","unstructured":"Cheng D, Bao C, Zhang X, Lin X, Huang H, Zhao L. LncRNA PRNCR1 interacts with HEY2 to abolish miR-448-mediated growth inhibition in non-small cell lung cancer. Biomed Pharmacother. 2018;107:1540\u20137.","journal-title":"Biomed Pharmacother"},{"issue":"15","key":"1691_CR21","doi-asserted-by":"publisher","first-page":"20584","DOI":"10.18632\/oncotarget.7828","volume":"7","author":"H Liu","year":"2016","unstructured":"Liu H, Li J, Koirala P, Ding X, Chen B, Wang Y, Wang Z, Wang C, Zhang X, Mo YY. Long non-coding RNAs as prognostic markers in human breast cancer. Oncotarget. 2016;7(15):20584\u201396.","journal-title":"Oncotarget"},{"issue":"41","key":"1691_CR22","doi-asserted-by":"publisher","first-page":"5422","DOI":"10.1038\/onc.2016.80","volume":"35","author":"F Liu","year":"2016","unstructured":"Liu F, Yuan JH, Huang JF, Yang F, Wang TT, Ma JZ, Zhang L, Zhou CC, Wang F, Yu J, et al. Long noncoding RNA FTX inhibits hepatocellular carcinoma proliferation and metastasis by binding MCM2 and miR-374a. Oncogene. 2016;35(41):5422\u201334.","journal-title":"Oncogene"},{"issue":"1","key":"1691_CR23","first-page":"48","volume":"23","author":"X Yu","year":"2018","unstructured":"Yu X, Zhao J, He Y. Long non-coding RNA PVT1 functions as an oncogene in human colon cancer through miR-30d-5p\/RUNX2 axis. J BUON. 2018;23(1):48\u201354.","journal-title":"J BUON"},{"issue":"12","key":"1691_CR24","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.1002\/cbin.11194","volume":"43","author":"YC Wang","year":"2019","unstructured":"Wang YC, He WY, Dong CH, Pei L, Ma YL. lncRNA HCG11 regulates cell progression by targeting miR-543 and regulating AKT\/mTOR pathway in prostate cancer. Cell Biol Int. 2019;43(12):1453\u201362.","journal-title":"Cell Biol Int"},{"issue":"3","key":"1691_CR25","doi-asserted-by":"publisher","first-page":"3097","DOI":"10.1007\/s13277-015-3987-9","volume":"37","author":"MH Ansari","year":"2016","unstructured":"Ansari MH, Irani S, Edalat H, Amin R, Mohammadi Roushandeh A. Deregulation of miR-93 and miR-143 in human esophageal cancer. Tumour Biol. 2016;37(3):3097\u2013103.","journal-title":"Tumour Biol"},{"issue":"9","key":"1691_CR26","doi-asserted-by":"publisher","first-page":"12011","DOI":"10.1007\/s13277-016-5065-3","volume":"37","author":"R Samsonov","year":"2016","unstructured":"Samsonov R, Burdakov V, Shtam T, Radzhabovsmall CZ, Vasilyev D, Tsyrlina E, Titov S, Ivanov M, Berstein L, Filatov M, et al. Plasma exosomal miR-21 and miR-181a differentiates follicular from papillary thyroid cancer. Tumour Biol. 2016;37(9):12011\u201321.","journal-title":"Tumour Biol"},{"key":"1691_CR27","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.yexmp.2018.12.010","volume":"107","author":"Z Mou","year":"2019","unstructured":"Mou Z, Wang Y, Li Y. Brazilein induces apoptosis and G1\/G0 phase cell cycle arrest by up-regulation of miR-133a in human vestibular schwannoma cells. Exp Mol Pathol. 2019;107:95\u2013101.","journal-title":"Exp Mol Pathol"},{"issue":"1","key":"1691_CR28","first-page":"550","volume":"18","author":"C Wu","year":"2019","unstructured":"Wu C, Zhao A, Tan T, Wang Y, Shen Z. Overexpression of microRNA-620 facilitates the resistance of triple negative breast cancer cells to gemcitabine treatment by targeting DCTD. Exp Ther Med. 2019;18(1):550\u20138.","journal-title":"Exp Ther Med"},{"issue":"11","key":"1691_CR29","first-page":"687","volume":"72","author":"N Li","year":"2017","unstructured":"Li N, Chen J, Zhao J, Wang T. MicroRNA-3188 targets ETS-domain protein 4 and participates in RhoA\/ROCK pathway to regulate the development of atherosclerosis. Pharmazie. 2017;72(11):687\u201393.","journal-title":"Pharmazie"},{"issue":"8","key":"1691_CR30","first-page":"555","volume":"1799","author":"S Kaikkonen","year":"2010","unstructured":"Kaikkonen S, Makkonen H, Rytinki M, Palvimo JJ. SUMOylation can regulate the activity of ETS-like transcription factor 4. Biochem Biophys Acta. 2010;1799(8):555\u201360.","journal-title":"Biochem Biophys Acta"}],"container-title":["Cancer Cell International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12935-020-01691-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12935-020-01691-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12935-020-01691-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T23:01:25Z","timestamp":1697497285000},"score":1,"resource":{"primary":{"URL":"https:\/\/cancerci.biomedcentral.com\/articles\/10.1186\/s12935-020-01691-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,5]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["1691"],"URL":"https:\/\/doi.org\/10.1186\/s12935-020-01691-0","relation":{},"ISSN":["1475-2867"],"issn-type":[{"value":"1475-2867","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,5]]},"assertion":[{"value":"19 May 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 January 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The informed consents had acquired from all participants in this clinical study, and protocol had approved by the Human Studies Committee of the First Affiliated Hospital of Kunming Medical University.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"5"}}