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CQ is suggested to accumulate in the parasite\u2019s digestive vacuole (DV), where it unfolds its anti-malarial properties. Discrepancies of CQ accumulation in CQ-sensitive (CQS) and CQ-resistant (CQR) strains are thought to play a significant role in drug susceptibility. Analysis of CQ transport and intracellular localization using a fluorescently tagged CQ analogue could provide much needed information to distinguish susceptible from resistant parasite strains. The fluorescently tagged CQ analogue LynxTag-CQ\u2122<jats:sub>GREEN<\/jats:sub> (CQ<jats:sub>GREEN<\/jats:sub>) is commercially available and was assessed for its suitability.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>IC<jats:sub>50<\/jats:sub> values were determined for both CQ and CQ<jats:sub>GREEN<\/jats:sub> in two CQS and two CQR <jats:italic>Plasmodium falciparum<\/jats:italic> strains. Buffer solutions with varying pH were used to determine pH-dependent localization of CQ<jats:sub>GREEN<\/jats:sub> in infected red blood cells. Before CQS or CQR parasites were exposed to different pH buffers, they\u00a0were pre-loaded with varying concentrations of CQ<jats:sub>GREEN<\/jats:sub> for up to 7\u00a0h. Intracellular accumulation was analysed using live cell confocal microscopy. CQ<jats:sub>GREEN<\/jats:sub> uptake rates were determined for the cytosol and DV in the presence and absence of verapamil.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In CQS strains, twofold higher IC<jats:sub>50<\/jats:sub> values were determined for the CQ<jats:sub>GREEN<\/jats:sub> analogue compared to CQ. No significant differences in IC<jats:sub>50<\/jats:sub> values were observed in CQR strains. Addition of verapamil reversed drug resistance of CQR strains to both CQ and CQ<jats:sub>GREEN<\/jats:sub>. Live cell imaging revealed that CQ<jats:sub>GREEN<\/jats:sub> fluorescence was mainly seen in the cytosol of most parasites, independent of the concentration used. Incubation periods of up to 7\u00a0h did not influence intracellular localization of CQ<jats:sub>GREEN<\/jats:sub>. Nevertheless, CQ<jats:sub>GREEN<\/jats:sub> uptake rates in CQR strains were reduced by 50% compared to CQS strains.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Although fluorescence of CQ<jats:sub>GREEN<\/jats:sub> was mainly seen in the cytosol of parasites, IC<jats:sub>50<\/jats:sub> assays showed comparable efficacy of CQ<jats:sub>GREEN<\/jats:sub> and CQ in parasite killing of CQS and CQR strains. Reduced uptake rates of CQ<jats:sub>GREEN<\/jats:sub> in CQR strains compared to CQS strains indicate parasite-specific responses to CQ<jats:sub>GREEN<\/jats:sub> exposure. The data contains valuable information when CQ<jats:sub>GREEN<\/jats:sub> is used as an analogue for CQ.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-019-2980-y","type":"journal-article","created":{"date-parts":[[2019,10,7]],"date-time":"2019-10-07T13:19:37Z","timestamp":1570454377000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Uptake of a fluorescently tagged chloroquine analogue is reduced in CQ-resistant compared to CQ-sensitive Plasmodium falciparum parasites"],"prefix":"10.1186","volume":"18","author":[{"given":"Sarah J.","family":"Reiling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7887-984X","authenticated-orcid":false,"given":"Petra","family":"Rohrbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,7]]},"reference":[{"key":"2980_CR1","volume-title":"World malaria report 2018","author":"WHO","year":"2018","unstructured":"WHO. 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