{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T11:53:11Z","timestamp":1780141991205,"version":"3.54.0"},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T00:00:00Z","timestamp":1598486400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T00:00:00Z","timestamp":1598486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Health and Medical Research Council, Australia","award":["1042072"],"award-info":[{"award-number":["1042072"]}]},{"name":"National Health and Medical Research Council, Australia","award":["1088738"],"award-info":[{"award-number":["1088738"]}]},{"name":"National Health and Medical Research Council, Australia","award":["1138860"],"award-info":[{"award-number":["1138860"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI116472-01"],"award-info":[{"award-number":["R01AI116472-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Australian Government Research Training Program (RTP) Scholarship"},{"name":"Australian Centre of Research Excellence in Malaria Elimination"},{"DOI":"10.13039\/501100006242","name":"Universiti Malaysia Sabah","doi-asserted-by":"crossref","award":["FRG0388"],"award-info":[{"award-number":["FRG0388"]}],"id":[{"id":"10.13039\/501100006242","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Malar J"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The monkey parasite\n                      <jats:italic>Plasmodium knowlesi<\/jats:italic>\n                      is an emerging public health issue in Southeast Asia. In Sabah, Malaysia,\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      is now the dominant cause of human malaria. Molecular detection methods for\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      are essential for accurate diagnosis and in monitoring progress towards malaria elimination of other\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      species. However, recent commercially available PCR malaria kits have unpublished\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      gene targets or have not been evaluated against clinical samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Two real-time PCR methods currently used in Sabah for confirmatory malaria diagnosis and surveillance reporting were evaluated: the QuantiFast\u2122 Multiplex PCR kit (Qiagen, Germany) targeting the\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      18S SSU rRNA; and the abTES\u2122 Malaria 5 qPCR II kit (AITbiotech, Singapore), with an undisclosed\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      gene target. Diagnostic accuracy was evaluated using 52\n                      <jats:italic>P. knowlesi,<\/jats:italic>\n                      25\n                      <jats:italic>Plasmodium vivax<\/jats:italic>\n                      , 21\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      , and 10\n                      <jats:italic>Plasmodium malariae<\/jats:italic>\n                      clinical isolates, and 26 malaria negative controls, and compared against a validated reference nested PCR assay. The limit of detection (LOD) for each PCR method and\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      species was also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The sensitivity of the QuantiFast\u2122 and abTES\u2122 assays for detecting\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      was comparable at 98.1% (95% CI 89.7\u2013100) and 100% (95% CI 93.2\u2013100), respectively. Specificity of the QuantiFast\u2122 and abTES\u2122 for\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      was high at 98.8% (95% CI 93.4\u2013100) for both assays. The QuantiFast\u2122 assay demonstrated falsely-positive mixed\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      species at low parasitaemias in both the primary and LOD analysis. Diagnostic accuracy of both PCR kits for detecting\n                      <jats:italic>P. vivax<\/jats:italic>\n                      ,\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      , and\n                      <jats:italic>P. malariae<\/jats:italic>\n                      was comparable to\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      . The abTES\u2122 assay demonstrated a lower LOD for\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      of\u2009\u2264\u20090.125 parasites\/\u00b5L compared to QuantiFast\u2122 with a LOD of 20 parasites\/\u00b5L. Hospital microscopy demonstrated a sensitivity of 78.8% (95% CI 65.3\u201388.9) and specificity of 80.4% (95% CI 67.6\u201389.8) compared to reference PCR for detecting\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The QuantiFast\u2122 and abTES\u2122 commercial PCR kits performed well for the accurate detection of\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      infections. Although the QuantiFast\u2122 kit is cheaper, the abTES\u2122 kit demonstrated a lower LOD, supporting its use as a second-line referral-laboratory diagnostic tool in Sabah, Malaysia.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-020-03379-2","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T11:03:49Z","timestamp":1598526229000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Comparative evaluation of two commercial real-time PCR kits (QuantiFast\u2122 and abTES\u2122) for the detection of Plasmodium knowlesi and other Plasmodium species in Sabah, Malaysia"],"prefix":"10.1186","volume":"19","author":[{"given":"Nor Afizah","family":"Nuin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7914-758X","authenticated-orcid":false,"given":"Angelica F.","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao Long","family":"Lew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim A.","family":"Piera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"William","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giri S.","family":"Rajahram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenarun","family":"Jelip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiloris F.","family":"Dony","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashidah","family":"Mohammad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridget E.","family":"Barber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas M.","family":"Anstey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tock H.","family":"Chua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Grigg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,27]]},"reference":[{"key":"3379_CR1","volume-title":"World malaria report 2019","author":"WHO","year":"2019","unstructured":"WHO. World malaria report 2019. Geneva: World Health Organization; 2019."},{"key":"3379_CR2","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1093\/cid\/ciz237","volume":"70","author":"DJ Cooper","year":"2020","unstructured":"Cooper DJ, Rajahram GS, William T, Jelip J, Mohammad R, Benedict J, et al. Plasmodium knowlesi malaria in Sabah, Malaysia, 2015\u20132017: ongoing increase in incidence despite near-elimination of the human-only Plasmodium species. Clin Infect Dis. 2020;70:361\u20137.","journal-title":"Clin Infect Dis"},{"key":"3379_CR3","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/s12936-020-3135-x","volume":"19","author":"N Hussin","year":"2020","unstructured":"Hussin N, Lim YA-L, Goh PP, William T, Jelip J, Mudin RN. Updates on malaria incidence and profile in Malaysia from 2013 to 2017. Malar J. 2020;19:55.","journal-title":"Malar J."},{"key":"3379_CR4","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/S0140-6736(04)15836-4","volume":"363","author":"B Singh","year":"2004","unstructured":"Singh B, Sung LK, Matusop A, Radhakrishnan A, Shamsul SS, Cox-Singh J, et al. A large focus of naturally acquired Plasmodium knowlesi infections in human beings. Lancet. 2004;363:1017\u201324.","journal-title":"Lancet"},{"key":"3379_CR5","doi-asserted-by":"crossref","first-page":"e0004915","DOI":"10.1371\/journal.pntd.0004915","volume":"10","author":"FM Shearer","year":"2016","unstructured":"Shearer FM, Huang Z, Weiss DJ, Wiebe A, Gibson HS, Battle KE, et al. Estimating geographical variation in the risk of zoonotic Plasmodium knowlesi infection in countries eliminating malaria. PLoS Negl Trop Dis. 2016;10:e0004915.","journal-title":"PLoS Negl Trop Dis."},{"key":"3379_CR6","doi-asserted-by":"crossref","first-page":"e0006244","DOI":"10.1371\/journal.pntd.0006244","volume":"12","author":"M Iwagami","year":"2018","unstructured":"Iwagami M, Nakatsu M, Khattignavong P, Soundala P, Lorphachan L, Keomalaphet S, et al. First case of human infection with Plasmodium knowlesi in Laos. PLoS Negl Trop Dis. 2018;12:e0006244.","journal-title":"PLoS Negl Trop Dis."},{"key":"3379_CR7","unstructured":"Ministry of Health Malaysia: Management guidelines of malaria in Malaysia. 2014."},{"key":"3379_CR8","unstructured":"WHO. Microscopy for the detection, identification and quantification of malaria parasites on stained thick and thin blood films in research settings. Geneva: World Health Organization; 2015. http:\/\/www.who.int\/tdr\/publications\/microscopy_detec_ident_quantif\/en."},{"key":"3379_CR9","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1475-2875-8-73","volume":"8","author":"K-S Lee","year":"2009","unstructured":"Lee K-S, Cox-Singh J, Singh B. Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections. Malar J. 2009;8:73.","journal-title":"Malar J."},{"key":"3379_CR10","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1475-2875-12-8","volume":"12","author":"BE Barber","year":"2013","unstructured":"Barber BE, William T, Grigg MJ, Yeo TW, Anstey NM. Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. Malar J. 2013;12:8.","journal-title":"Malar J."},{"key":"3379_CR11","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/CMR.00079-12","volume":"26","author":"B Singh","year":"2013","unstructured":"Singh B, Daneshvar C. Human infections and detection of Plasmodium knowlesi. Clin Microbiol Rev. 2013;26:165\u201384.","journal-title":"Clin Microbiol Rev"},{"key":"3379_CR12","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1186\/1475-2875-13-390","volume":"13","author":"T William","year":"2014","unstructured":"William T, Jelip J, Menon J, Anderios F, Mohammad R, Mohammad TAA, et al. Changing epidemiology of malaria in Sabah, Malaysia: increasing incidence of Plasmodium knowlesi. Malar J. 2014;13:390.","journal-title":"Malar J."},{"key":"3379_CR13","doi-asserted-by":"crossref","first-page":"e0006924","DOI":"10.1371\/journal.pntd.0006924","volume":"12","author":"FN Coutrier","year":"2018","unstructured":"Coutrier FN, Tirta YK, Cotter C, Zarlinda I, Gonz\u00e1lez IJ, Schwartz A, et al. Laboratory challenges of Plasmodium species identification in Aceh Province, Indonesia, a malaria elimination setting with newly discovered P. knowlesi. PLoS Negl Trop Dis. 2018;12:e0006924.","journal-title":"PLoS Negl Trop Dis."},{"key":"3379_CR14","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.3201\/eid1411.080840","volume":"14","author":"TF McCutchan","year":"2008","unstructured":"McCutchan TF, Piper RC, Makler MT. Use of malaria rapid diagnostic test to identify Plasmodium knowlesi infection. Emerg Infect Dis. 2008;14:1750.","journal-title":"Emerg Infect Dis"},{"key":"3379_CR15","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.parint.2009.06.004","volume":"58","author":"S Kawai","year":"2009","unstructured":"Kawai S, Hirai M, Haruki K, Tanabe K, Chigusa Y. Cross-reactivity in rapid diagnostic tests between human malaria and zoonotic simian malaria parasite Plasmodium knowlesi infections. Parasitol Int. 2009;58:300\u20132.","journal-title":"Parasitol Int"},{"key":"3379_CR16","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1128\/JCM.03285-12","volume":"51","author":"BE Barber","year":"2013","unstructured":"Barber BE, William T, Grigg MJ, Piera K, Yeo TW, Anstey NM. Evaluation of the sensitivity of a pLDH-based and an aldolase-based rapid diagnostic test for diagnosis of uncomplicated and severe malaria caused by PCR-confirmed Plasmodium knowlesi, Plasmodium falciparum, and Plasmodium vivax. J Clin Microbiol. 2013;51:1118\u201323.","journal-title":"J Clin Microbiol"},{"key":"3379_CR17","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1128\/JCM.00181-14","volume":"52","author":"MJ Grigg","year":"2014","unstructured":"Grigg MJ, William T, Barber BE, Parameswaran U, Bird E, Piera K, et al. Combining parasite lactate dehydrogenase-based and histidine-rich protein 2-based rapid tests to improve specificity for diagnosis of malaria due to Plasmodium knowlesi and other Plasmodium species in Sabah, Malaysia. J Clin Microbiol. 2014;52:2053\u201360.","journal-title":"J Clin Microbiol"},{"key":"3379_CR18","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/1475-2875-13-60","volume":"13","author":"D Foster","year":"2014","unstructured":"Foster D, Cox-Singh J, Mohamad DS, Krishna S, Chin PP, Singh B. Evaluation of three rapid diagnostic tests for the detection of human infections with Plasmodium knowlesi. Malar J. 2014;13:60.","journal-title":"Malar J."},{"key":"3379_CR19","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1093\/infdis\/jiy520","volume":"219","author":"NM Anstey","year":"2019","unstructured":"Anstey NM, Grigg MJ. Zoonotic malaria: the better you look, the more you find. J Infect Dis. 2019;219:679\u201381.","journal-title":"J Infect Dis"},{"key":"3379_CR20","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1186\/1475-2875-9-344","volume":"9","author":"PC Divis","year":"2010","unstructured":"Divis PC, Shokoples SE, Singh B, Yanow SK. A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi. Malar J. 2010;9:344.","journal-title":"Malar J."},{"key":"3379_CR21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1179\/000349803125002977","volume":"97","author":"D Padley","year":"2003","unstructured":"Padley D, Moody A, Chiodini P, Saldanha J. Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present. Ann Trop Med Parasitol. 2003;97:131\u20137.","journal-title":"Ann Trop Med Parasitol"},{"key":"3379_CR22","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1128\/JCM.00811-09","volume":"47","author":"M Imwong","year":"2009","unstructured":"Imwong M, Tanomsing N, Pukrittayakamee S, Day NP, White NJ, Snounou G. Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P knowlesi. J Clin Microbiol. 2009;47:4173\u20135.","journal-title":"J Clin Microbiol"},{"key":"3379_CR23","volume-title":"Guidelines for the treatment of malaria","author":"WHO","year":"2015","unstructured":"WHO. Guidelines for the treatment of malaria. Geneva: World Health Organization; 2015."},{"key":"3379_CR24","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1128\/JCM.42.12.5636-5643.2004","volume":"42","author":"M Rougemont","year":"2004","unstructured":"Rougemont M, Van Saanen M, Sahli R, Hinrikson HP, Bille J, Jaton K. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. J Clin Microbiol. 2004;42:5636\u201343.","journal-title":"J Clin Microbiol"},{"key":"3379_CR25","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1056\/NEJM197507312930501","volume":"293","author":"BJ McNeil","year":"1975","unstructured":"McNeil BJ, Keeler E, Adelstein SJ. Primer on certain elements of medical decision making. N Engl J Med. 1975;293:211\u20135.","journal-title":"N Engl J Med"},{"key":"3379_CR26","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1259\/bjr.70.832.9166071","volume":"70","author":"N Hawass","year":"1997","unstructured":"Hawass N. Comparing the sensitivities and specificities of two diagnostic procedures performed on the same group of patients. Br J Radiol. 1997;70:360\u20136.","journal-title":"Br J Radiol"},{"key":"3379_CR27","doi-asserted-by":"crossref","first-page":"e31848","DOI":"10.1371\/journal.pone.0031848","volume":"7","author":"NW Lucchi","year":"2012","unstructured":"Lucchi NW, Poorak M, Oberstaller J, DeBarry J, Srinivasamoorthy G, Goldman I, et al. A new single-step PCR assay for the detection of the zoonotic malaria parasite Plasmodium knowlesi. PLoS One. 2012;7:e31848.","journal-title":"PLoS One"},{"key":"3379_CR28","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1475-2875-12-26","volume":"12","author":"CG Haanshuus","year":"2013","unstructured":"Haanshuus CG, Mohn SC, M\u00f8rch K, Langeland N, Blomberg B, Hanevik K. A novel, single-amplification PCR targeting mitochondrial genome highly sensitive and specific in diagnosing malaria among returned travellers in Bergen, Norway. Malar J. 2013;12:26.","journal-title":"Malar J."},{"key":"3379_CR29","doi-asserted-by":"crossref","first-page":"207","DOI":"10.3906\/sag-1411-114","volume":"46","author":"CS Stanis","year":"2016","unstructured":"Stanis CS, Song BK, Chua TH, Lau YL, Jelip J. Evaluation of new multiplex PCR primers for the identification of Plasmodium species found in Sabah, Malaysia. Turkish J Med Sci. 2016;46:207\u201318.","journal-title":"Turkish J Med Sci."},{"key":"3379_CR30","doi-asserted-by":"crossref","first-page":"2931","DOI":"10.1128\/JCM.00958-13","volume":"51","author":"ME Reller","year":"2013","unstructured":"Reller ME, Chen WH, Dalton J, Lichay MA, Dumler JS. Multiplex 5\u2032 nuclease quantitative real-time PCR for clinical diagnosis of malaria and species-level identification and epidemiologic evaluation of malaria-causing parasites, including Plasmodium knowlesi. J Clin Microbiol. 2013;51:2931\u20138.","journal-title":"J Clin Microbiol"},{"key":"3379_CR31","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s12936-015-0542-5","volume":"14","author":"PC Lee","year":"2015","unstructured":"Lee PC, Chong ETJ, Anderios F, Lim YA, Chew CH, Chua KH. Molecular detection of human Plasmodium species in Sabah using PlasmoNex\u2122 multiplex PCR and hydrolysis probes real-time PCR. Malar J. 2015;14:28.","journal-title":"Malar J."},{"key":"3379_CR32","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1186\/1475-2875-10-197","volume":"10","author":"Y-L Lau","year":"2011","unstructured":"Lau Y-L, Fong M-Y, Mahmud R, Chang P-Y, Palaeya V, Cheong F-W, et al. Specific, sensitive and rapid detection of human Plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples. Malar J. 2011;10:197.","journal-title":"Malar J."},{"key":"3379_CR33","doi-asserted-by":"crossref","first-page":"120","DOI":"10.4269\/ajtmh.15-0670","volume":"95","author":"S Britton","year":"2016","unstructured":"Britton S, Cheng Q, Grigg MJ, William T, Anstey NM, McCarthy JS. A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of Plasmodium knowlesi. Am J Trop Med Hyg. 2016;95:120\u20132.","journal-title":"Am J Trop Med Hyg"},{"key":"3379_CR34","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1128\/JCM.00331-10","volume":"48","author":"H Iseki","year":"2010","unstructured":"Iseki H, Kawai S, Takahashi N, Hirai M, Tanabe K, Yokoyama N, et al. Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi. J Clin Microbiol. 2010;48:2509\u201314.","journal-title":"J Clin Microbiol"},{"key":"3379_CR35","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s12936-016-1676-9","volume":"16","author":"KA Piera","year":"2017","unstructured":"Piera KA, Aziz A, William T, Bell D, Gonz\u00e1lez IJ, Barber BE, et al. Detection of Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax using loop-mediated isothermal amplification (LAMP) in a co-endemic area in Malaysia. Malar J. 2017;16:29.","journal-title":"Malar J."},{"key":"3379_CR36","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1128\/JCM.06859-11","volume":"50","author":"L Link","year":"2012","unstructured":"Link L, Bart A, Verhaar N, van Gool T, Pronk M, Scharnhorst V. Molecular detection of Plasmodium knowlesi in a Dutch traveler by real-time PCR. J Clin Microbiol. 2012;50:2523\u20134.","journal-title":"J Clin Microbiol"},{"key":"3379_CR37","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/expr.1995.1107","volume":"81","author":"J Li","year":"1995","unstructured":"Li J, Wirtz RA, Mcconkey GA, Sattabongkot J, Waters AP, Rogers MJ, et al. Plasmodium: genus-conserved primers for species identification and quantitation. Exp Parasitol. 1995;81:182\u201390.","journal-title":"Exp Parasitol"},{"key":"3379_CR38","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1093\/nar\/17.5.2135","volume":"17","author":"A Waters","year":"1989","unstructured":"Waters A, McCutchan T. Partial sequence of the asexually expressed SU rRNA gene of Plasmodium vivax. Nucleic Acids Res. 1989;17:2135.","journal-title":"Nucleic Acids Res"},{"key":"3379_CR39","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1093\/cid\/cis902","volume":"56","author":"BE Barber","year":"2013","unstructured":"Barber BE, William T, Grigg MJ, Menon J, Auburn S, Marfurt J, et al. A prospective comparative study of knowlesi, falciparum, and vivax malaria in Sabah, Malaysia: high proportion with severe disease from Plasmodium knowlesi and Plasmodium vivax but no mortality with early referral and artesunate therapy. Clin Infect Dis. 2013;56:383\u201397.","journal-title":"Clin Infect Dis"},{"key":"3379_CR40","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1093\/cid\/ciy065","volume":"67","author":"MJ Grigg","year":"2018","unstructured":"Grigg MJ, William T, Barber BE, Rajahram GS, Menon J, Schimann E, et al. Age-related clinical spectrum of Plasmodium knowlesi malaria and predictors of severity. Clin Infect Dis. 2018;67:350\u20139.","journal-title":"Clin Infect Dis"},{"key":"3379_CR41","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/S1473-3099(15)00415-6","volume":"16","author":"MJ Grigg","year":"2016","unstructured":"Grigg MJ, William T, Menon J, Dhanaraj P, Barber BE, Wilkes CS, et al. Artesunate\u2013mefloquine versus chloroquine for treatment of uncomplicated Plasmodium knowlesi malaria in Malaysia (ACT KNOW): an open-label, randomised controlled trial. Lancet Infect Dis. 2016;16:180\u20138.","journal-title":"Lancet Infect Dis."},{"key":"3379_CR42","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1093\/infdis\/jiz397","volume":"220","author":"L Grignard","year":"2019","unstructured":"Grignard L, Shah S, Chua TH, William T, Drakeley CJ, Fornace KM. Natural human infections with Plasmodium cynomolgi and other malaria species in an elimination setting in Sabah, Malaysia. J Infect Dis. 2019;220:1946\u20139.","journal-title":"J Infect Dis"},{"key":"3379_CR43","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/1475-2875-13-68","volume":"13","author":"TH Ta","year":"2014","unstructured":"Ta TH, Hisam S, Lanza M, Jiram AI, Ismail N, Rubio JM. First case of a naturally acquired human infection with Plasmodium cynomolgi. Malar J. 2014;13:68.","journal-title":"Malar J."},{"key":"3379_CR44","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1093\/infdis\/jiy519","volume":"219","author":"M Imwong","year":"2018","unstructured":"Imwong M, Madmanee W, Suwannasin K, Kunasol C, Peto TJ, Tripura R, et al. Asymptomatic natural human infections with the simian malaria parasites Plasmodium cynomolgi and Plasmodium knowlesi. J Infect Dis. 2018;219:695\u2013702.","journal-title":"J Infect Dis"},{"key":"3379_CR45","volume-title":"The primate malarias","author":"GR Coatney","year":"1971","unstructured":"Coatney GR, Collins WE, Warren M, Contacos PG. The primate malarias. Bethesda MD: US Department of Health Education and Welfare; 1971."},{"key":"3379_CR46","doi-asserted-by":"crossref","first-page":"15671","DOI":"10.1038\/srep15671","volume":"5","author":"KH Chua","year":"2015","unstructured":"Chua KH, Lim SC, Ng CC, Lee PC, Lim YAL, Lau TP, et al. Development of high resolution melting analysis for the diagnosis of human malaria. Sci Rep. 2015;5:15671.","journal-title":"Sci Rep."}],"container-title":["Malaria Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12936-020-03379-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12936-020-03379-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12936-020-03379-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T20:05:27Z","timestamp":1630008327000},"score":1,"resource":{"primary":{"URL":"https:\/\/malariajournal.biomedcentral.com\/articles\/10.1186\/s12936-020-03379-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,27]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["3379"],"URL":"https:\/\/doi.org\/10.1186\/s12936-020-03379-2","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-30644\/v2","asserted-by":"object"},{"id-type":"doi","id":"10.21203\/rs.3.rs-30644\/v1","asserted-by":"object"},{"id-type":"doi","id":"10.21203\/rs.3.rs-30644\/v3","asserted-by":"object"}]},"ISSN":["1475-2875"],"issn-type":[{"value":"1475-2875","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,27]]},"assertion":[{"value":"23 May 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 August 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This study was approved by the Medical Research and Ethics Committee, Ministry of Health, Malaysia (NMRR-10-754-6684), and Menzies School of Health Research, Australia (HREC-2010-1431).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"306"}}