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In the context of the disease elimination by 2030, a nationwide entomological survey was conducted to better understand the distribution, abundance and behaviour of major malaria vectors (\n                      <jats:italic>Anopheles<\/jats:italic>\n                      spp.) in the country.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Mosquito collections were implemented in ten villages from ten provinces during the rainy and dry seasons of 2014 and 2015 by using human landing catch (HLC) and cow bait collection (CBC) methods. After morphological identification in the field, molecular identification of the sibling species of\n                      <jats:italic>Anopheles<\/jats:italic>\n                      mosquitoes from the Funestus, Leucosphyrus, and Maculatus groups were determined using PCR specific alleles. A screening of\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      and\n                      <jats:italic>Plasmodium vivax<\/jats:italic>\n                      infections in the vectors was carried out by quantitative PCR assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 14,146 adult mosquitoes representing 25 different\n                      <jats:italic>Anopheles<\/jats:italic>\n                      species were collected and morphologically identified. Molecular identification revealed the presence of 12 sibling species within the main primary vector groups, including\n                      <jats:italic>Anopheles maculatus<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles rampae<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles sawadwongporni<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles pseudowillmori<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles dravidicus<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles minimus<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles aconitus<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles pampanai<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles harrisoni<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles dirus<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles baimaii<\/jats:italic>\n                      ,\n                      <jats:italic>Anopheles nemophilous<\/jats:italic>\n                      .\n                      <jats:italic>Anopheles maculatus<\/jats:italic>\n                      and\n                      <jats:italic>An. minimus<\/jats:italic>\n                      were predominant during both the dry and rainy seasons, but showed highly zoophilic preferences (Zoophilic index of 98% and 95%, respectively). Overall, 22% of the total malaria vectors were collected between 10:00 PM and 5:00 AM indoors when people are sleeping. Twenty-seven percent of primary and secondary vectors were collected outdoors before 10:00 PM or after 5:00 AM, times when people are usually awake and outdoors. Only two specimens were positive for\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      , one\n                      <jats:italic>An. aconitus<\/jats:italic>\n                      from Phongsaly and one\n                      <jats:italic>An. minimus<\/jats:italic>\n                      from Vientiane Province\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results indicate that people living in rural areas in Laos are constantly exposed to malaria vectors throughout the year and specifically outdoors. The use of LLINs\/IRS remains important but innovative tools and new strategies are needed to address locally, the early and outdoor malaria transmission. Lack of expertise in general entomological methods may further exacerbate the situation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-020-03453-9","type":"journal-article","created":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T18:02:36Z","timestamp":1604944956000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["New insights into malaria vector bionomics in Lao PDR: a nationwide entomology survey"],"prefix":"10.1186","volume":"19","author":[{"given":"S\u00e9bastien","family":"Marcombe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santi","family":"Maithaviphet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Bobichon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nothasin","family":"Phommavan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Nambanya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Corbel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.","family":"Brey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,9]]},"reference":[{"key":"3453_CR1","volume-title":"World malaria report 2019","author":"WHO","year":"2019","unstructured":"WHO. 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