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Serological surveillance will be particularly important in areas of low or periodic transmission because patient antibody responses can provide a measure of historical exposure. While methods for detecting host antibody responses to <jats:italic>Plasmodium falciparum<\/jats:italic> and <jats:italic>Plasmodium vivax<\/jats:italic> are well established, development of serological assays for <jats:italic>Plasmodium knowlesi<\/jats:italic>, <jats:italic>Plasmodium ovale<\/jats:italic> and <jats:italic>Plasmodium malariae<\/jats:italic> have been inhibited by a lack of immunodiagnostic candidates due to the limited availability of genomic information.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Using the recently completed genome sequences from <jats:italic>P. malariae<\/jats:italic>, <jats:italic>P. ovale<\/jats:italic> and <jats:italic>P. knowlesi,<\/jats:italic> a set of 33 candidate cell surface and secreted blood-stage antigens was selected and expressed in a recombinant form using a mammalian expression system. These proteins were added to an existing panel of antigens from <jats:italic>P. falciparum<\/jats:italic> and <jats:italic>P. vivax<\/jats:italic> and the immunoreactivity of IgG, IgM and IgA immunoglobulins from individuals diagnosed with infections to each of the five different <jats:italic>Plasmodium<\/jats:italic> species was evaluated by ELISA. Logistic regression modelling was used to quantify the ability of the responses to determine prior exposure to the different <jats:italic>Plasmodium<\/jats:italic> species.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Using sera from European travellers with diagnosed <jats:italic>Plasmodium<\/jats:italic> infections, antigens showing species-specific immunoreactivity were identified to select a panel of 22 proteins from five <jats:italic>Plasmodium<\/jats:italic> species for serological profiling. The immunoreactivity to the antigens in the panel of sera taken from travellers and individuals living in malaria-endemic regions with diagnosed infections showed moderate power to predict infections by each species, including <jats:italic>P. ovale<\/jats:italic>, <jats:italic>P. malariae<\/jats:italic> and <jats:italic>P. knowlesi.<\/jats:italic> Using a larger set of patient samples and logistic regression modelling it was shown that exposure to <jats:italic>P. knowlesi<\/jats:italic> could be accurately detected (AUC\u2009=\u200991%) using an antigen panel consisting of the <jats:italic>P. knowlesi<\/jats:italic> orthologues of MSP10, P12 and P38.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Using the recent availability of genome sequences to all human-infective <jats:italic>Plasmodium<\/jats:italic> spp. parasites and a method of expressing <jats:italic>Plasmodium<\/jats:italic> proteins in a secreted functional form, an antigen panel has been compiled that will be useful to determine exposure to these parasites.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-020-3111-5","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T14:02:50Z","timestamp":1579269770000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance"],"prefix":"10.1186","volume":"19","author":[{"given":"Nicole","family":"M\u00fcller-Sienerth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarrod","family":"Shilts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khamisah Abdul","family":"Kadir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Yman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manijeh Vafa","family":"Homann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Asghar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Billy","family":"Ngasala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balbir","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"F\u00e4rnert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0537-0863","authenticated-orcid":false,"given":"Gavin J.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,17]]},"reference":[{"key":"3111_CR1","volume-title":"World Malaria Report","author":"WHO","year":"2018","unstructured":"WHO. 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