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Here, the epidemiology and detectability of asymptomatic\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      and\n                      <jats:italic>Plasmodium vivax<\/jats:italic>\n                      infections were investigated in different transmission settings in Ethiopia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>A total of 1093 dried blood spot (DBS) samples were collected from afebrile and apparently healthy individuals across ten study sites in Ethiopia from 2016 to 2020. Of these, 862 were from community and 231 from school based cross-sectional surveys. Malaria infection status was determined by microscopy or rapid diagnostics tests (RDT) and 18S rRNA-based nested PCR (nPCR). The annual parasite index (API) was used to classify endemicity as low (API\u2009&gt;\u20090 and &lt;\u20095), moderate (API\u2009\u2265\u20095 and &lt;\u2009100) and high transmission (API\u2009\u2265\u2009100) and detectability of infections was assessed in these settings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In community surveys, the overall prevalence of asymptomatic\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      infections by microscopy\/RDT, nPCR and all methods combined was 12.2% (105\/860), 21.6% (183\/846) and 24.1% (208\/862), respectively. The proportion of nPCR positive infections that was detectable by microscopy\/RDT was 48.7% (73\/150) for\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      and 4.6% (2\/44) for\n                      <jats:italic>P. vivax<\/jats:italic>\n                      . Compared to low transmission settings, the likelihood of detecting infections by microscopy\/RDT was increased in moderate (Adjusted odds ratio [AOR]: 3.4; 95% confidence interval [95% CI] 1.6\u20137.2, P\u2009=\u20090.002) and high endemic settings (AOR\u2009=\u20095.1; 95% CI\u20092.6\u20139.9, P\u2009&lt;\u20090.001). After adjustment for site and correlation between observations from the same survey, the likelihood of detecting asymptomatic infections by microscopy\/RDT (AOR per year increase\u2009=\u20090.95, 95% CI\u20090.9\u20131.0, P\u2009=\u20090.013) declined with age.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Conventional diagnostics missed nearly half of the asymptomatic\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      reservoir detected by nPCR. The detectability of infections was particularly low in older age groups and low transmission settings. These findings highlight the need for sensitive diagnostic tools to detect the entire parasite reservoir and potential infection transmitters.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-021-03587-4","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T02:03:21Z","timestamp":1611281001000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["The epidemiology and detectability of asymptomatic Plasmodium vivax and Plasmodium falciparum infections in low, moderate and high transmission settings in Ethiopia"],"prefix":"10.1186","volume":"20","author":[{"given":"Elifaged","family":"Hailemeskel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surafel K","family":"Tebeje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinknesh W.","family":"Behaksra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girma","family":"Shumie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Getasew","family":"Shitaye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Migbaru","family":"Keffale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wakweya","family":"Chali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abrham","family":"Gashaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temesgen","family":"Ashine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Drakeley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teun","family":"Bousema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Endalamaw","family":"Gadisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1931-1442","authenticated-orcid":false,"given":"Fitsum G.","family":"Tadesse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,22]]},"reference":[{"key":"3587_CR1","unstructured":"WHO. 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