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This study was designed to investigate the genetic diversity of\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      isolates from the semi-arid area in North East Ethiopia, using the highly polymorphic merozoite surface protein-2 (\n                      <jats:italic>msp2<\/jats:italic>\n                      ) gene as a molecular marker.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Dried blood spot isolates were collected from patients with\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      infection between September 2014 and January 2015 from Melka-Werer, North East Ethiopia. Parasite DNA was extracted and genotyped using allele-specific nested polymerase chain reactions for\n                      <jats:italic>msp2<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      52 isolates were collected with\n                      <jats:italic>msp2<\/jats:italic>\n                      identified in 41 (78.8%) isolates. Allele typing of the\n                      <jats:italic>msp2<\/jats:italic>\n                      gene detected the 3D7\/IC allelic family in 54% and FC27 allelic family in 46%. A total of 14 different\n                      <jats:italic>msp2<\/jats:italic>\n                      genotypes were detected including 6 belonging to the 3D7\/IC family and 8 to the FC27 family. Forty percent of isolates had multiple genotypes and the overall mean multiplicity of infections (MOI) was 1.2 (95%CI 0.96\u20131.42). The heterozygosity index was 0.50 for the\n                      <jats:italic>msp2<\/jats:italic>\n                      locus. There was no difference in MOI between age groups. A negative correlation between parasite density and multiplicity of infection was found (p\u2009=\u20090.02).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      isolates from the semi-arid area of North East Ethiopia are mainly monoclonal with low MOI and limited genetic diversity in the study population.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-021-03625-1","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T15:35:32Z","timestamp":1613144132000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Genetic polymorphisms of Plasmodium falciparum isolates from Melka-Werer, North East Ethiopia based on the merozoite surface protein-2 (msp-2) gene as a molecular marker"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5677-9522","authenticated-orcid":false,"given":"Hussein","family":"Mohammed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashenafi","family":"Assefa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melkie","family":"Chernet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonas","family":"Wuletaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Commons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"key":"3625_CR1","unstructured":"WHO. 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