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The study describes single nucleotide polymorphisms in Kelch protein propeller domain of <jats:italic>P. falciparum<\/jats:italic> associated with artemisinin resistance from Southern Pakistan.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Two hundred and forty-nine samples were collected from patients with microscopy confirmed <jats:italic>P. falciparum<\/jats:italic> malaria attending Aga Khan University Hospital during September 2015-April 2018. DNA was isolated using the whole blood protocol for the QIAmp DNA Blood Kit. The <jats:italic>k13<\/jats:italic> propeller gene (<jats:italic>k13<\/jats:italic>) was amplified using nested PCR. Double-strand sequencing of PCR products was performed using Sanger sequencing methodology. Sequences were analysed with MEGA 6 and Bio edit software to identify specific SNP combinations.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>All isolates analysed for <jats:italic>k13<\/jats:italic> propeller allele were observed as wild-type in samples collected post implementation of ACT in Pakistan. C580Y, A675V, Y493H and R539T\u00a0variants associated with reduced susceptibility to artemisinin-based combination therapy (ACT) were not found. Low frequency of M476I and C469Y polymorphisms was found, which is significantly associated with artemisinin resistance.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Low frequencies of both nonsynonymous and synonymous polymorphisms were observed in <jats:italic>P. falciparum<\/jats:italic> isolates circulating in Southern Pakistan. The absence of known molecular markers of artemisinin resistance in this region is favourable for anti-malarial efficacy of ACT. Surveillance of anti-malarial drug resistance to detect its emergence and spread need to be strengthened in Pakistan.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-021-03715-0","type":"journal-article","created":{"date-parts":[[2021,4,7]],"date-time":"2021-04-07T13:06:05Z","timestamp":1617800765000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Molecular surveillance of drug resistance: Plasmodium falciparum artemisinin resistance single nucleotide polymorphisms in Kelch protein propeller (K13) domain from Southern Pakistan"],"prefix":"10.1186","volume":"20","author":[{"given":"Najia Karim","family":"Ghanchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bushra","family":"Qurashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadiqa","family":"Raees","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Asim","family":"Beg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,4,7]]},"reference":[{"key":"3715_CR1","unstructured":"WHO. 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