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Clinical manifestation of reported malaria cases in Aceh varied widely from asymptomatic, mild uncomplicated to severe and fatal complications. The present study aims to explore the allelic diversity of merozoite surface protein 1\u00a0gene (\n                      <jats:italic>msp1<\/jats:italic>\n                      ) and\n                      <jats:italic>msp2<\/jats:italic>\n                      among the\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      isolates in Aceh Province and to determine their potential correlation with the severity of malaria clinical manifestation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Screening of over 500 malaria cases admitted to the hospitals in 11 districts hospital within Aceh Province during 2013\u20132015, identified 90 cases of\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      mono-infection without any co-morbidity. The subjects were clinically phenotyped and parasite DNA was extracted and polymerase chain reaction (PCR) amplified for the\n                      <jats:italic>msp1<\/jats:italic>\n                      \u00a0and\n                      <jats:italic>msp2<\/jats:italic>\n                      \u00a0allelic subfamilies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Analysis of clinical manifestation revealed that fever-chill is the most frequent symptom. Based on WHO criteria showed 19 cases were classified as severe and 71 as mild malaria. Analysis of\n                      <jats:italic>msp1<\/jats:italic>\n                      gene revealed the presence of K1 allele subfamily in 34 subjects, MAD20 in 42 subjects, RO33 in 1 subject, and mixed allelic of K1\u2009+\u2009MAD20 in 5 subjects, K1\u2009+\u2009RO33 in 4 subjects, and MAD20\u2009+\u2009RO33 in 4 subjects. Analysis of\n                      <jats:italic>msp2<\/jats:italic>\n                      gene revealed 34 subjects carried the FC27 allelic subfamily, 37 subjects carried the 3D7 and 19 subjects carried the mixed FC27\u2009+\u20093D7. Analysis of multiplicity of infection revealed that\n                      <jats:italic>msp1<\/jats:italic>\n                      \u00a0alleles is slightly higher than\n                      <jats:italic>msp2<\/jats:italic>\n                      with the mean of MOI were 2.69 and 2.27, respectively. Statistical analysis to determine the association between each clinical manifestation and\n                      <jats:italic>msp1<\/jats:italic>\n                      and\n                      <jats:italic>msp2<\/jats:italic>\n                      alleles revealed that liver function abnormal value was associated with the\n                      <jats:italic>msp2<\/jats:italic>\n                      mixed alleles (odds ratio (OR):0.13; 95%CI: 0.03\u20130.53). Mixed\n                      <jats:italic>msp1<\/jats:italic>\n                      \u00a0of K1\u2009+\u2009RO33 was associated with severe malaria (OR: 28.50; 95%CI: 1.59\u20131532.30).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study found a strong association between severe malaria in Aceh with subjects carrying the\n                      <jats:italic>msp1<\/jats:italic>\n                      mixed alleles of K1 and RO33. The liver function abnormal value associated with the\n                      <jats:italic>msp2<\/jats:italic>\n                      mixed allelic subfamilies. Further study in different geographic areas is recommended.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-021-03719-w","type":"journal-article","created":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T08:28:28Z","timestamp":1618302508000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Allelic diversity of merozoite surface protein genes (msp1 and msp2) and clinical manifestations of Plasmodium falciparum malaria cases in Aceh, Indonesia"],"prefix":"10.1186","volume":"20","author":[{"given":"Kurnia Fitri","family":"Jamil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandha Rizki","family":"Pratama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia Sance","family":"Marantina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harapan","family":"Harapan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Riza","family":"Kurniawan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tjut Mariam","family":"Zanaria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jontari","family":"Hutagalung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail Ekoprayitno","family":"Rozi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puji Budi Setia","family":"Asih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Supargiyono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Din","family":"Syafruddin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,13]]},"reference":[{"key":"3719_CR1","unstructured":"Ministry of Health, Republic of Indonesia. 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