{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:27:56Z","timestamp":1791959276146,"version":"4.3.4"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Translational Research Development for Endemic Infectious Diseases of Amazonia \u2013 FOGARTY","award":["2D43TW007120-11A1"],"award-info":[{"award-number":["2D43TW007120-11A1"]}]},{"name":"Project Belgian Development Cooperation (DGD) through the collaborative project Framework Agreement 4"},{"name":"The COBIAN scholarship of the Facultad de Ciencias y Filosof\u00eda de la UPCH"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Malar J"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Loop-mediated isothermal amplification (LAMP) for malaria diagnosis at the point of care (POC) depends on the detection capacity of synthesized nucleic acids and the specificity of the amplification target. To improve malaria diagnosis, new colorimetric LAMP tests were developed using multicopy targets for <jats:italic>Plasmodium vivax<\/jats:italic> and <jats:italic>Plasmodium falciparum<\/jats:italic> detection.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The cytochrome oxidase I (<jats:italic>COX1<\/jats:italic>) mitochondrial gene and the non-coding sequence <jats:italic>Pvr47<\/jats:italic> for <jats:italic>P. vivax<\/jats:italic>, and the sub-telomeric sequence of erythrocyte membrane protein 1 (<jats:italic>EMP1<\/jats:italic>) and the non-coding sequence <jats:italic>Pfr364<\/jats:italic> for <jats:italic>P. falciparum<\/jats:italic> were targeted to design new LAMP primers. The limit of detection (LOD) of each colorimetric LAMP was established and assessed with DNA extracted by mini spin column kit and the Boil &amp; Spin method from 28 microscopy infections, 101 malaria submicroscopic infections detected by real-time PCR only, and 183 negatives infections by both microscopy and PCR.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The LODs for the colorimetric LAMPs were estimated between 2.4 to 3.7 parasites\/\u00b5L of whole blood. For <jats:italic>P. vivax<\/jats:italic> detection, the colorimetric LAMP using the COX1 target showed a better performance than the <jats:italic>Pvr47<\/jats:italic> target, whereas the <jats:italic>Pfr364<\/jats:italic> target was the most specific for <jats:italic>P. falciparum<\/jats:italic> detection. All microscopic infections of <jats:italic>P. vivax<\/jats:italic> were detected by PvCOX1-LAMP using the mini spin column kit DNA extraction method and 81% (17\/21) were detected using Boil &amp; Spin sample preparation. Moreover, all microscopic infections of <jats:italic>P. falciparum<\/jats:italic> were detected by Pfr364-LAMP using both sample preparation methods. In total, PvCOX1-LAMP and Pfr364-LAMP detected 80.2% (81 samples) of the submicroscopic infections using the DNA extraction method by mini spin column kit, while 36.6% (37 samples) were detected using the Boil &amp; Spin sample preparation method.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The colorimetric LAMPs with multicopy targets using the <jats:italic>COX1<\/jats:italic> target for <jats:italic>P. vivax<\/jats:italic> and the <jats:italic>Pfr364<\/jats:italic> for <jats:italic>P. falciparum<\/jats:italic> have a high potential to improve POC malaria diagnosis detecting a greater number of submicroscopic <jats:italic>Plasmodium<\/jats:italic> infections.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-021-03753-8","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T19:03:47Z","timestamp":1621451027000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5672-5516","authenticated-orcid":false,"given":"Oscar","family":"Nolasco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhoel","family":"Montoya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L.","family":"Rosales Rosas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scarlett","family":"Barrientos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Rosanas-Urgell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionicia","family":"Gamboa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"key":"3753_CR1","volume-title":"World malaria report 2020","author":"WHO","year":"2020","unstructured":"WHO. World malaria report 2020. Geneva: World Health Organization; 2020."},{"key":"3753_CR2","unstructured":"Centro Nacional de Epidemiolog\u00eda, Prevenci\u00f3n y Control de Enfermedades. Situaci\u00f3n de la malaria en el Per\u00fa. Boletin Epidemiol\u00f3gico 02-2021. https:\/\/www.dge.gob.pe\/epipublic\/uploads\/boletin\/boletin_20212.pdf. Accessed 17 Mar 2021."},{"key":"3753_CR3","doi-asserted-by":"publisher","first-page":"e0005674","DOI":"10.1371\/journal.pntd.0005674","volume":"11","author":"E Rovira-Vallbona","year":"2017","unstructured":"Rovira-Vallbona E, Contreras-Mancilla JJ, Ramirez R, Guzman-Guzman M, Carrasco-Escobar G, Llanos-Cuentas A, et al. Predominance of asymptomatic and sub-microscopic infections characterizes the Plasmodium gametocyte reservoir in the Peruvian Amazon. PLoS Negl Trop Dis. 2017;11:e0005674.","journal-title":"PLoS Negl Trop Dis"},{"key":"3753_CR4","doi-asserted-by":"publisher","first-page":"1509","DOI":"10.1086\/644781","volume":"200","author":"LC Okell","year":"2009","unstructured":"Okell LC, Ghani AC, Lyons E, Drakeley CJ. Submicroscopic infection in Plasmodium falciparum-endemic populations: a systematic review and meta-analysis. J Infect Dis. 2009;200:1509\u201317.","journal-title":"J Infect Dis"},{"key":"3753_CR5","unstructured":"Ministerio de Salud del Per\u00fa. Norma t\u00e9cnica para la atenci\u00f3n de la malaria y malaria severa en el Per\u00fa. NTS Nro. 054-MINSA\/DGSP-V.01, modificada en Febrero 2015. MINSA; 2015. http:\/\/bvs.minsa.gob.pe\/local\/MINSA\/4373.pdf. Accessed 17 Mar 2021."},{"key":"3753_CR6","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1046\/j.1365-2257.1999.00220.x","volume":"21","author":"T H\u00e4nscheid","year":"1999","unstructured":"H\u00e4nscheid T. Diagnosis of malaria: a review of alternatives to conventional microscopy. Clin Lab Haematol. 1999;21:235\u201345.","journal-title":"Clin Lab Haematol"},{"key":"3753_CR7","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1046\/j.1365-3156.2000.00509.x","volume":"5","author":"AH Kilian","year":"2000","unstructured":"Kilian AH, Metzger WG, Mutschelknauss EJ, Kabagambe G, Langi P, Korte R, et al. Reliability of malaria microscopy in epidemiological studies: results of quality control. Trop Med Int Health. 2000;5:3\u20138.","journal-title":"Trop Med Int Health"},{"key":"3753_CR8","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/s11908-018-0655-4","volume":"20","author":"AN Mukkala","year":"2018","unstructured":"Mukkala AN, Kwan J, Lau R, Harris D, Kain D, Boggild AK. An update on malaria rapid diagnostic tests. Curr Infect Dis Rep. 2018;20:49.","journal-title":"Curr Infect Dis Rep"},{"key":"3753_CR9","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1186\/1475-2875-12-181","volume":"12","author":"JW Jang","year":"2013","unstructured":"Jang JW, Cho CH, Han ET, An SS, Lim CS. pLDH level of clinically isolated Plasmodium vivax and detection limit of pLDH based malaria rapid diagnostic test. Malar J. 2013;12:181.","journal-title":"Malar J"},{"key":"3753_CR10","doi-asserted-by":"publisher","first-page":"e43094","DOI":"10.1371\/journal.pone.0043094","volume":"7","author":"J Maltha","year":"2012","unstructured":"Maltha J, Gamboa D, Bendezu J, Sanchez L, Cnops L, Gillet P, et al. Rapid diagnostic tests for malaria diagnosis in the Peruvian Amazon: impact of pfhrp2 gene deletions and cross-reactions. PLoS ONE. 2012;7:e43094.","journal-title":"PLoS ONE"},{"key":"3753_CR11","doi-asserted-by":"publisher","first-page":"e8091","DOI":"10.1371\/journal.pone.0008091","volume":"5","author":"D Gamboa","year":"2010","unstructured":"Gamboa D, Ho MF, Bendezu J, Torres K, Chiodini PL, Barnwell JW, et al. A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests. PLoS ONE. 2010;5:e8091.","journal-title":"PLoS ONE"},{"key":"3753_CR12","doi-asserted-by":"publisher","first-page":"e0137458","DOI":"10.1371\/journal.pone.0137458","volume":"10","author":"A Rosas-Aguirre","year":"2015","unstructured":"Rosas-Aguirre A, Speybroeck N, Llanos-Cuentas A, Rosanas-Urgell A, Carrasco-Escobar G, Rodriguez H, et al. Hotspots of malaria transmission in the Peruvian Amazon: rapid assessment through a parasitological and serological survey. PLoS ONE. 2015;10:e0137458.","journal-title":"PLoS ONE"},{"key":"3753_CR13","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1186\/s12936-017-1957-y","volume":"16","author":"A Rosas-Aguirre","year":"2017","unstructured":"Rosas-Aguirre A, Guzman-Guzman M, Gamboa D, Chuquiyauri R, Ramirez R, Manrique P, et al. Micro-heterogeneity of malaria transmission in the Peruvian Amazon: a baseline assessment underlying a population-based cohort study. Malar J. 2017;16:312.","journal-title":"Malar J"},{"key":"3753_CR14","doi-asserted-by":"publisher","first-page":"1466","DOI":"10.1093\/infdis\/jiaa526","volume":"223","author":"A Rosas-Aguirre","year":"2020","unstructured":"Rosas-Aguirre A, Guzman-Guzman M, Chuquiyauri R, Moreno M, Manrique P, Ramirez R, et al. Temporal and micro-spatial heterogeneity in transmission dynamics of co-endemic Plasmodium vivax and Plasmodium falciparum in two rural cohort populations in the Peruvian Amazon. J Infect Dis. 2020;223:1466\u201377.","journal-title":"J Infect Dis"},{"key":"3753_CR15","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1186\/s12936-017-2063-x","volume":"16","author":"G Carrasco-Escobar","year":"2017","unstructured":"Carrasco-Escobar G, Miranda-Alban J, Fernandez-Minope C, Brouwer KC, Torres K, Calderon M, et al. High prevalence of very-low Plasmodium falciparum and Plasmodium vivax parasitaemia carriers in the Peruvian Amazon: insights into local and occupational mobility-related transmission. Malar J. 2017;16:415.","journal-title":"Malar J"},{"key":"3753_CR16","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1016\/S0020-7519(02)00111-X","volume":"32","author":"O Mercereau-Puijalon","year":"2002","unstructured":"Mercereau-Puijalon O, Barale J, Bischoff E. Three multigene families in Plasmodium parasites: facts and questions. Int J Parasitol. 2002;32:1323\u201344.","journal-title":"Int J Parasitol"},{"key":"3753_CR17","doi-asserted-by":"publisher","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:E63.","journal-title":"Nucleic Acids Res"},{"key":"3753_CR18","doi-asserted-by":"publisher","first-page":"167","DOI":"10.2144\/000113072","volume":"3","author":"M Goto","year":"2009","unstructured":"Goto M, Honda E, Ogura A, Nomoto A, Hanaki K. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques. 2009;3:167\u201372.","journal-title":"Biotechniques"},{"key":"3753_CR19","doi-asserted-by":"publisher","first-page":"59","DOI":"10.2144\/000114253","volume":"58","author":"NA Tanner","year":"2015","unstructured":"Tanner NA, Zhang Y, Evans TC Jr. Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes. Biotechniques. 2015;58:59\u201368.","journal-title":"Biotechniques"},{"key":"3753_CR20","doi-asserted-by":"publisher","first-page":"2866","DOI":"10.1128\/JCM.00355-10","volume":"48","author":"SD Polley","year":"2010","unstructured":"Polley SD, Mori Y, Watson J, Perkins MD, Gonzalez IJ, Notomi T, et al. Mitochondrial DNA targets increase sensitivity of malaria detection using loop-mediated isothermal amplification. J Clin Microbiol. 2010;48:2866\u201371.","journal-title":"J Clin Microbiol"},{"key":"3753_CR21","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1093\/infdis\/jit184","volume":"208","author":"H Hopkins","year":"2013","unstructured":"Hopkins H, Gonz\u00e1lez I, Polley S, Angutoko P, Ategeka J, Asiimwe C, et al. Highly sensitive detection of malaria parasitemia in a malaria-endemic setting: performance of a new loop-mediated isothermal amplification kit in a remote clinic in Uganda. J Infect Dis. 2013;208:645\u201352.","journal-title":"J Infect Dis"},{"key":"3753_CR22","first-page":"11","volume":"15","author":"IJ Gonz\u00e1lez","year":"2012","unstructured":"Gonz\u00e1lez IJ, Polley S, Hopkins H, Sutherland C, Mori Y, Perkins M, Bell D. Molecular diagnosis for screening and elimination of malaria: performance of the first commercially-available malaria LAMP test. Malar J. 2012;15:11.","journal-title":"Malar J"},{"key":"3753_CR23","doi-asserted-by":"publisher","first-page":"e103905","DOI":"10.1371\/journal.pone.0103905","volume":"9","author":"B Aydin-Schmidt","year":"2014","unstructured":"Aydin-Schmidt B, Xu W, Gonz\u00e1lez I, Polley S, Bell D, Shakely D, et al. Loop mediated isothermal amplification (LAMP) accurately detects malaria DNA from filter paper blood samples of low density parasitaemias. PLoS ONE. 2014;9:e103905.","journal-title":"PLoS ONE"},{"key":"3753_CR24","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1186\/s12936-016-1158-0","volume":"15","author":"S Britton","year":"2016","unstructured":"Britton S, Cheng Q, McCarthy JS. Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings. Malar J. 2016;15:88.","journal-title":"Malar J"},{"key":"3753_CR25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4137\/IDRT.S32162","volume":"9","author":"SS Modak","year":"2016","unstructured":"Modak SS, Barber CA, Geva E, Abrams WR, Malamud D, Ongagna YS. Rapid point-of-care isothermal amplification assay for the detection of malaria without nucleic acid purification. Infect Dis (Auckl). 2016;9:1\u20139.","journal-title":"Infect Dis (Auckl)"},{"key":"3753_CR26","doi-asserted-by":"publisher","first-page":"36808","DOI":"10.1038\/srep36808","volume":"6","author":"NW Lucchi","year":"2016","unstructured":"Lucchi NW, Gaye M, Diallo MA, Goldman IF, Ljolje D, Deme AB, et al. Evaluation of the illumigene malaria LAMP: a robust molecular diagnostic tool for malaria parasites. Sci Rep. 2016;6:36808.","journal-title":"Sci Rep"},{"key":"3753_CR27","doi-asserted-by":"publisher","first-page":"e0151437","DOI":"10.1371\/journal.pone.0151437","volume":"11","author":"NW Lucchi","year":"2016","unstructured":"Lucchi NW, Ljolje D, Silva-Flannery L, Udhayakumar V. Use of malachite green-loop mediated isothermal amplification for detection of Plasmodium spp. parasites. PLoS ONE. 2016;11:e0151437.","journal-title":"PLoS ONE"},{"key":"3753_CR28","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1186\/s12879-017-2718-9","volume":"17","author":"K Imai","year":"2017","unstructured":"Imai K, Tarumoto N, Misawa K, Runtuwene LR, Sakai J, Hayashida K, et al. A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinIONTM nanopore sequencer. BMC Infect Dis. 2017;17:621.","journal-title":"BMC Infect Dis"},{"key":"3753_CR29","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1186\/s12936-019-2979-4","volume":"18","author":"AN Mohon","year":"2019","unstructured":"Mohon AN, Getie S, Jahan N, Alam MS, Pillai DR. Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination. Malar J. 2019;18:350.","journal-title":"Malar J"},{"key":"3753_CR30","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1186\/s12936-019-2722-1","volume":"18","author":"H Kudyba","year":"2019","unstructured":"Kudyba H, Louzada J, Ljolje D, Kudyba K, Muralidharan V, Oliveira-Ferreira J, et al. Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil. Malar J. 2019;18:98.","journal-title":"Malar J"},{"key":"3753_CR31","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1186\/s12936-020-03283-9","volume":"19","author":"D Selvarajah","year":"2020","unstructured":"Selvarajah D, Naing C, Htet NH, Mak JW. Loop-mediated isothermal amplification (LAMP) test for diagnosis of uncomplicated malaria in endemic areas: a meta-analysis of diagnostic test accuracy. Malar J. 2020;19:211.","journal-title":"Malar J"},{"key":"3753_CR32","doi-asserted-by":"publisher","first-page":"e0234263","DOI":"10.1371\/journal.pone.0234263","volume":"6","author":"KA Barazorda","year":"2020","unstructured":"Barazorda KA, Salas CJ, Bishop DK, Lucchi N, Valdivia HO. Comparison of realtime and malachite-green based loop-mediated isothermal amplification assays for the detection of Plasmodium vivax and P. falciparum. PLoS ONE. 2020;6:e0234263.","journal-title":"PLoS ONE"},{"key":"3753_CR33","doi-asserted-by":"publisher","first-page":"e0185742","DOI":"10.1371\/journal.pone.0185742","volume":"12","author":"E Serra-Casas","year":"2017","unstructured":"Serra-Casas E, Manrique P, Ding X, Carrasco-Escobar G, Alava F, Gave A, et al. Loop-mediated isothermal DNA amplification for asymptomatic malaria detection in challenging field settings: technical performance and pilot implementation in the Peruvian Amazon. PLoS ONE. 2017;12:e0185742.","journal-title":"PLoS ONE"},{"key":"3753_CR34","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.4269\/ajtmh.20-0212","volume":"4","author":"O Nolasco","year":"2020","unstructured":"Nolasco O, Infante B, Contreras-Mancilla, Incardona S, Ding XC, Gamboa D, et al. Diagnosis of Plasmodium vivax by loop-mediated isothermal amplification in febrile patient samples from Loreto, Per\u00fa. Am J Trop Med Hyg. 2020;4:1549\u201352.","journal-title":"Am J Trop Med Hyg"},{"key":"3753_CR35","doi-asserted-by":"publisher","first-page":"e1001788","DOI":"10.1371\/journal.pmed.1001788","volume":"12","author":"N Hofmann","year":"2015","unstructured":"Hofmann N, Mwingira F, Shekalaghe S, Robinson LJ, Mueller I, Felger I. Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets. PLoS Med. 2015;12:e1001788.","journal-title":"PLoS Med"},{"key":"3753_CR36","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/s12936-018-2201-0","volume":"17","author":"M Gruenberg","year":"2018","unstructured":"Gruenberg M, Moniz CA, Hofmann NE, Wampfler R, Koepfli C, Mueller I, et al. Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions. Malar J. 2018;17:55.","journal-title":"Malar J"},{"key":"3753_CR37","doi-asserted-by":"publisher","first-page":"2411","DOI":"10.1128\/JCM.02603-10","volume":"49","author":"A Demas","year":"2011","unstructured":"Demas A, Oberstaller J, DeBarry J, Lucchi NW, Srinivasamoorthy G, Sumari D, et al. Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. J Clin Microbiol. 2011;49:2411\u20138.","journal-title":"J Clin Microbiol"},{"key":"3753_CR38","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1186\/s12936-019-2781-3","volume":"18","author":"LC Amaral","year":"2019","unstructured":"Amaral LC, Robortella DR, Guimar\u00e3es LFF, Limongi JE, Fontes CJF, Pereira DB, et al. Ribosomal and non-ribosomal PCR targets for the detection of low-density and mixed malaria infections. Malar J. 2019;18:154.","journal-title":"Malar J"},{"key":"3753_CR39","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1186\/s12936-019-2743-9","volume":"18","author":"B Grabias","year":"2019","unstructured":"Grabias B, Essuman E, Quakyi IA, Kumar S. Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification. Malar J. 2019;18:116.","journal-title":"Malar J"},{"key":"3753_CR40","doi-asserted-by":"publisher","first-page":"2435","DOI":"10.1128\/JCM.43.5.2435-2440.2005","volume":"43","author":"KA Mangold","year":"2005","unstructured":"Mangold KA, Manson RU, Koay ESC, Stephens L, Regner M, Thomson RB, et al. Real-time PCR for detection and identification of Plasmodium spp. J Clin Microbiol. 2005;43:2435\u201340.","journal-title":"J Clin Microbiol"},{"key":"3753_CR41","doi-asserted-by":"publisher","first-page":"5636","DOI":"10.1128\/JCM.42.12.5636-5643.2004","volume":"42","author":"M Rougemont","year":"2004","unstructured":"Rougemont M, Van Saanen M, Sahli R, Hinrikson HP, Bille J, Jaton K. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. J Clin Microbiol. 2004;42:5636\u201343.","journal-title":"J Clin Microbiol"},{"key":"3753_CR42","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1111\/lam.12675","volume":"64","author":"I Choopara","year":"2017","unstructured":"Choopara I, Arunrut N, Kiatpathomchai W, Dean D, Somboonna N. Rapid and visual Chlamydia trachomatis detection using loop-mediated isothermal amplification and hydroxynaphthol blue. Lett Appl Microbiol. 2017;64:51\u20136.","journal-title":"Lett Appl Microbiol"},{"key":"3753_CR43","doi-asserted-by":"publisher","first-page":"2521","DOI":"10.1128\/JCM.02117-06","volume":"45","author":"E-T Han","year":"2007","unstructured":"Han E-T, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, et al. Detection of four Plasmodium species by genus-and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol. 2007;45:2521\u20138.","journal-title":"J Clin Microbiol"},{"key":"3753_CR44","doi-asserted-by":"publisher","first-page":"e54986","DOI":"10.1371\/journal.pone.0054986","volume":"8","author":"J Patel","year":"2013","unstructured":"Patel J, Oberstaller J, Xayavong M, Narayanan J, DeBarry J, Srinivasamoorthy G, et al. Real-time loop-mediated isothermal amplification (RealAmp) for the species-specific identification of Plasmodium vivax. PLoS ONE. 2013;8:e54986.","journal-title":"PLoS ONE"},{"key":"3753_CR45","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1186\/1475-2875-13-248","volume":"13","author":"S Dinzouna-Boutamba","year":"2014","unstructured":"Dinzouna-Boutamba S, Yang H, Joo S, Jeong S, Na B, Inoue N, et al. The development of loop-mediated isothermal amplification targeting alpha-tubulin DNA for the rapid detection of Plasmodium vivax. Malar J. 2014;13:248.","journal-title":"Malar J"},{"key":"3753_CR46","doi-asserted-by":"publisher","first-page":"e0004443","DOI":"10.1371\/journal.pntd.0004443","volume":"10","author":"S Britton","year":"2016","unstructured":"Britton S, Cheng Q, Grigg M, Poole C, Pasay C, William T, et al. Sensitive detection of Plasmodium vivax using a high-throughput, colourimetric loop mediated isothermal amplification (HtLAMP) platform: a potential novel tool for malaria elimination. PLoS Negl Trop Dis. 2016;10:e0004443.","journal-title":"PLoS Negl Trop Dis"},{"key":"3753_CR47","doi-asserted-by":"publisher","first-page":"e2780","DOI":"10.1371\/journal.pntd.0002780","volume":"8","author":"CL Moyes","year":"2014","unstructured":"Moyes CL, Henry AJ, Golding N, Huang Z, Singh B, Baird JK, et al. Defining the geographical range of the Plasmodium knowlesi reservoir. PLoS Negl Trop Dis. 2014;8:e2780.","journal-title":"PLoS Negl Trop Dis"}],"container-title":["Malaria Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12936-021-03753-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12936-021-03753-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12936-021-03753-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T19:04:27Z","timestamp":1621451067000},"score":1,"resource":{"primary":{"URL":"https:\/\/malariajournal.biomedcentral.com\/articles\/10.1186\/s12936-021-03753-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,19]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["3753"],"URL":"https:\/\/doi.org\/10.1186\/s12936-021-03753-8","relation":{},"ISSN":["1475-2875"],"issn-type":[{"value":"1475-2875","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,19]]},"assertion":[{"value":"21 January 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 May 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical approval was obtained from the corresponding Ethical Review board from Universidad Peruana Cayetano Heredia (SIDISI Project: 201296 and Constancy letter: E070-14-20).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All authors have reviewed the manuscript and consented for publication.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"225"}}