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This study aimed to determine the intensity of vector resistance to insecticides in three regions of Benin with different agro-ecological characteristics.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Larvae of <jats:italic>Anopheles gambiae <\/jats:italic>sensu lato (<jats:italic>s.l<\/jats:italic>.) were collected from September to November 2017 in different larval sites in three northern Benin communes: Parakou, Kandi and Malanville. Two to five-day-old, non-blood-fed, female mosquitoes were exposed to papers impregnated with deltamethrin, permethrin and bendiocarb at dosages of 1\u2009\u00d7\u2009the diagnostic dose, 5\u2009\u00d7\u2009and 10\u2009\u00d7\u2009to determine the intensity of resistance in these vectors. Molecular frequencies of the <jats:italic>kdr<\/jats:italic> L1014F and <jats:italic>ace-1R<\/jats:italic> G119S insecticide resistance mutations and levels of detoxification enzymes were determined for mosquitoes sampled at each study site.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Resistance to pyrethroids (permethrin and deltamethrin) was recorded in all three communes with mortality rates below 60% using the diagnostic dose (1x). The results obtained after exposure of <jats:italic>An. gambiae<\/jats:italic> to permethrin 10\u2009\u00d7\u2009were 99% in Kandi, 98% in Malanville and 99% in Parakou. With deltamethrin 10x, mortality rates were 100% in Kandi, 96% in Malanville and 73% in Parakou. For the diagnostic dose of bendiocarb, suspected resistance was recorded in the communes of Malanville (97%) and Kandi (94%) while sensitivity was observed in Parakou (98%).Using the 10\u2009\u00d7\u2009dose, mortality was 98% in Kandi, 100% in Malanville and 99% in Parakou. The frequencies of the <jats:italic>kdr<\/jats:italic> L1014F allele varied between 59 and 83% depending on the sites and species of the <jats:italic>An. gambiae<\/jats:italic> complex, while the frequency of the <jats:italic>ace-1R<\/jats:italic> G119S gene varied between 0 and 5%. Biochemical tests showed high levels of oxidase and esterase activity compared to the susceptible colony strain of <jats:italic>An. gambiae <\/jats:italic>sensu stricto (Kisumu strain).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p><jats:italic>Anopheles gambiae<\/jats:italic> showed a generalized loss of susceptibility to permethrin and deltamethrin but also showed moderate to high intensity of resistance in different regions of Benin. This high intensity of resistance is a potential threat to the effectiveness of vector control.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-021-03856-2","type":"journal-article","created":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T10:03:28Z","timestamp":1627380208000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa"],"prefix":"10.1186","volume":"20","author":[{"given":"Casimir Dossou","family":"Kpanou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann W.","family":"Sagbohan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fortun\u00e9","family":"Dagnon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Germain G.","family":"Padonou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Razaki","family":"Oss\u00e8","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert Sourou","family":"Salako","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aboubakar","family":"Sidick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfried","family":"Sewad\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Sominahouin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Condo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saadani Hassani","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Impoinvil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Akogb\u00e9to","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,27]]},"reference":[{"key":"3856_CR1","unstructured":"WHO: World malaria report 2017. 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