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Falciparum malaria is a major public health threat to human life responsible for high mortality. Currently, the risk of multi-drug resistance of\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      is rapidly increasing. There is a need to address new anti-malarial therapeutics strategies to combat the drug-resistance threat.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      essential proteins were retrieved from the recently published studies\n                      <jats:italic>.<\/jats:italic>\n                      These proteins were initially scanned against human host and its gut microbiome proteome sets by comparative proteomics analyses. The human host non-homologs essential proteins of\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      were additionally analysed for druggability potential via in silico methods to possibly identify novel therapeutic targets. Finally, the PfAp4AH target was prioritized for pharmacophore modelling based virtual screening and molecular docking analyses to identify potent inhibitors from drug-like compounds databases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The analyses identified six\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      essential and human host non-homolog proteins that follow the key druggability features. These druggable targets have not been catalogued so far in the Drugbank repository. These prioritized proteins seem novel and promising drug targets against\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      due to their key protein\u2013protein interactions features in pathogen-specific biological pathways and to hold appropriate drug-like molecule binding pockets. The pharmacophore features based virtual screening of Pharmit\u00a0resource predicted a lead compound i.e. MolPort-045\u2013917-542 as a promising inhibitor of PfAp4AH among prioritized targets.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The prioritized protein targets may worthy to test in malarial drug discovery programme to overcome the anti-malarial resistance issues. The\n                      <jats:italic>in-vitro<\/jats:italic>\n                      and\n                      <jats:italic>in-vivo<\/jats:italic>\n                      studies might be promising for additional validation of these prioritized lists of drug targets against malaria.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-021-03865-1","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T01:36:24Z","timestamp":1628818584000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Analysing the essential proteins set of Plasmodium falciparum PF3D7 for novel drug targets identification against malaria"],"prefix":"10.1186","volume":"20","author":[{"given":"Fawad","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hira","family":"Wali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saadia","family":"Jan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asad","family":"Zia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneeba","family":"Aslam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imtiaz","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahib Gul","family":"Afridi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulaiman","family":"Shams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1444-4249","authenticated-orcid":false,"given":"Asifullah","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,3]]},"reference":[{"key":"3865_CR1","first-page":"73","volume":"12","author":"AO Bakhraibah","year":"2018","unstructured":"Bakhraibah AO. 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