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This study presents the comparative evaluation of two multiplex platforms in identifying <jats:italic>Plasmodium falciparum<\/jats:italic> with presence or absence of HRP2\/HRP3 expression as being indicative of <jats:italic>hrp2\/hrp3<\/jats:italic> deletions and other <jats:italic>Plasmodium<\/jats:italic> species. Moreover, correlation between the malaria antigen measurements performed at these platforms is assessed after calibrating with either assay standards or international standards and the cross-reactivity among <jats:italic>Plasmodium<\/jats:italic> species is examined.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A 77-member panel of specimens composed of the World Health Organization (WHO) international <jats:italic>Plasmodium<\/jats:italic> antigen standards, cultured parasites for <jats:italic>P. falciparum<\/jats:italic> and <jats:italic>Plasmodium knowlesi<\/jats:italic>, and clinical specimens with mono-infections for <jats:italic>P. falciparum<\/jats:italic>, <jats:italic>Plasmodium vivax<\/jats:italic>, and <jats:italic>Plasmodium malariae<\/jats:italic> was generated as both whole blood and dried blood spot (DBS) specimens. Assays for HRP2, <jats:italic>P. falciparum<\/jats:italic>\u2013specific pLDH (<jats:italic>Pf<\/jats:italic>LDH), <jats:italic>P. vivax<\/jats:italic>\u2013specific pLDH (<jats:italic>Pv<\/jats:italic>LDH), and all human <jats:italic>Plasmodium<\/jats:italic> species Pan malaria pLDH (PanLDH) on the Human Malaria Array Q-Plex and the xMAP platforms were evaluated with these panels.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The xMAP showed a higher percent positive agreement for identification of <jats:italic>hrp2<\/jats:italic>-deleted <jats:italic>P. falciparum<\/jats:italic> and <jats:italic>Plasmodium<\/jats:italic> species in whole blood and DBS than the Q-Plex. For whole blood samples, there was a highly positive correlation between the two platforms for <jats:italic>Pf<\/jats:italic>LDH (Pearson <jats:italic>r<\/jats:italic>\u2009=\u20090.9926) and <jats:italic>Pv<\/jats:italic>LDH (<jats:italic>r<\/jats:italic>\u2009=\u20090. 9792), moderate positive correlation for HRP2 (<jats:italic>r<\/jats:italic>\u2009=\u20090.7432), and poor correlation for PanLDH (<jats:italic>r<\/jats:italic>\u2009=\u20090.6139). In Pearson correlation analysis between the two platforms on the DBS, the same assays were <jats:italic>r<\/jats:italic>\u2009=\u20090.9828, <jats:italic>r<\/jats:italic>\u2009=\u20090.7679, <jats:italic>r<\/jats:italic>\u2009=\u20090.6432, and <jats:italic>r<\/jats:italic>\u2009=\u20090.8957, respectively. The xMAP HRP2 assay appeared to cross-react with HRP3, while the Q-Plex did not. The Q-Plex <jats:italic>Pf<\/jats:italic>LDH assay cross-reacted with <jats:italic>P. malariae<\/jats:italic>, while the xMAP did not. For both platforms, <jats:italic>P. knowlesi<\/jats:italic> was detected on the <jats:italic>Pv<\/jats:italic>LDH assay. The WHO international standards allowed normalization across both platforms on their HRP2, <jats:italic>Pf<\/jats:italic>LDH, and <jats:italic>Pv<\/jats:italic>LDH assays in whole blood and DBS.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Q-Plex and xMAP show good agreement for identification of <jats:italic>P. falciparum<\/jats:italic> mutants with <jats:italic>hrp2\/hrp3<\/jats:italic> deletions, and other <jats:italic>Plasmodium<\/jats:italic> species. Quantitative results from both platforms, normalized into international units for HRP2, <jats:italic>Pf<\/jats:italic>LDH, and <jats:italic>Pv<\/jats:italic>LDH, showed good agreement and should allow comparison and analysis of results generated by either platform.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-022-04203-9","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T11:04:26Z","timestamp":1654599866000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens"],"prefix":"10.1186","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8899-7930","authenticated-orcid":false,"given":"Ihn Kyung","family":"Jang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfons","family":"Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Rashid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Barney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison","family":"Golden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier C.","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gonzalo J.","family":"Domingo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Mayor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"4203_CR1","doi-asserted-by":"publisher","first-page":"2009","DOI":"10.1128\/JCM.02562-16","volume":"55","author":"BA Mathison","year":"2017","unstructured":"Mathison BA, Pritt BS. 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