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Currently, the World Health Organization (WHO) recommends SP-IPTp in the second and third trimesters. However, the efficacy of SP-IPTp is threatened by the emergence of sulfadoxine-pyrimethamine resistant malaria parasites due to single nucleotide polymorphisms in the <jats:italic>Plasmodium falciparum<\/jats:italic> dihydrofolate reductase and dihydropteroate synthetase genes. This study aimed to assess the current prevalence of <jats:italic>Pfdhfr\/Pfdhps<\/jats:italic> mutations in <jats:italic>P. falciparum<\/jats:italic> isolates collected from individuals residing in Ile-Ife, Nigeria, and also present maps of the prevalence of <jats:italic>Pfdhps<\/jats:italic> 431V and 581G within Nigeria and surrounding countries.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Between October 2020 and April 2021, samples were collected as dried blood spots among 188 participants who showed malaria positivity with a histidine-rich-protein-based rapid diagnostic test (RDT). Nested PCR assays were used to confirm falciparum in the samples with RDT positivity, and to amplify fragments of the <jats:italic>Pfdhfr\/Pfdhps<\/jats:italic> genes followed by targeted amplicon sequencing. Published data since 2007 on the prevalence of the <jats:italic>Pfdhps<\/jats:italic> genotypes in Nigeria and the neighbouring countries were used to produce maps to show the distribution of the mutant genotypes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Only 74 and 61 samples were successfully amplified for the <jats:italic>Pfdhfr<\/jats:italic> and <jats:italic>Pfdhps<\/jats:italic> genes, respectively. At codons resulting in N51I, C59R, and S108N, <jats:italic>Pfdhfr<\/jats:italic> carried mutant alleles of 97.3% (72\/74), 97.3% (72\/74) and 98.6% (73\/74), respectively. The <jats:italic>Pfdhps<\/jats:italic> gene carried mutations at codons resulting in amino acid changes at 431\u2013436-437\u2013540-581\u2013613; I431V [45.9%, (28\/61)], A581G [31.1% (19\/61)] and A613S [49.2% (30\/61)]. Constructed haplotypes were mainly the triple <jats:italic>Pfdhfr<\/jats:italic> mutant 51I-59R-108N (95.9%), and the most common haplotypes observed for the <jats:italic>Pfdhps<\/jats:italic> gene were the IS<jats:bold>G<\/jats:bold>KAA (32.8%), IS<jats:bold>G<\/jats:bold>K<jats:bold>GS<\/jats:bold> (8.2%), <jats:bold>V<\/jats:bold>A<jats:bold>G<\/jats:bold>KAA (14.8%), <jats:bold>V<\/jats:bold>A<jats:bold>G<\/jats:bold>KA<jats:bold>S<\/jats:bold> (9.8%) and <jats:bold>V<\/jats:bold>A<jats:bold>G<\/jats:bold>K<jats:bold>GS<\/jats:bold> (14.8%). In the context of the previously published data, a high prevalence of 431V\/581G mutations was found in the study population. It seems quite evident that the <jats:italic>Pfdhps<\/jats:italic> 431V, 581G and 613S often co-occur as <jats:italic>Pfdhps<\/jats:italic>-<jats:bold>V<\/jats:bold>A<jats:bold>G<\/jats:bold>K<jats:bold>GS<\/jats:bold> haplotype.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study showed that the prevalence of <jats:bold>V<\/jats:bold>A<jats:bold>G<\/jats:bold>K<jats:bold>GS<\/jats:bold> haplotype seems to be increasing in prevalence. If this is similar in effect to the emergence of 581G in East Africa, the efficacy of SP-IPTp in the presence of these novel <jats:italic>Pfdhps<\/jats:italic> mutants should be re-assessed.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-023-04487-5","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T10:03:14Z","timestamp":1677664994000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["A snapshot of the prevalence of dihydropteroate synthase-431V mutation and other sulfadoxine-pyrimethamine resistance markers in Plasmodium falciparum isolates in Nigeria"],"prefix":"10.1186","volume":"22","author":[{"given":"Adebanjo J.","family":"Adegbola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omotade A.","family":"Ijarotimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akaninyene E.","family":"Ubom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bukola A.","family":"Adesoji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olajide E.","family":"Babalola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma F.","family":"Hocke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helle","family":"Hansson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andria","family":"Mousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluseye O.","family":"Bolaji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Alifrangis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cally","family":"Roper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,1]]},"reference":[{"key":"4487_CR1","volume-title":"World Malaria Report","author":"WHO","year":"2021","unstructured":"WHO. 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