{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T08:57:49Z","timestamp":1790413069116,"version":"4.1.0"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the Norwegian Programme for Capacity Development in Higher Education and Research for Development-Arba Minch University project","award":["ETH-13\/0025"],"award-info":[{"award-number":["ETH-13\/0025"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Malar J"],"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Plasmodium falciparum<\/jats:italic>\n and\n Plasmodium vivax<\/jats:italic>\n are coendemic in Ethiopia, with different proportion in different settings. Microscopy is the diagnostic tool in Ethiopian health centres. Accurate species-specific diagnosis is vital for appropriate treatment of cases to interrupt its transmission. Therefore, this study assessed the status of species-specific misdiagnosis by microscope compared with polymerase chain reaction (PCR).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A health facility based cross-sectional study was conducted from November 2019 to January 2020 in Kolla Shelle Health centre, Arba Minch Zuria district. The study population were suspected malaria cases, who visited the health centre for a diagnosis and treatment. Consecutive microscopy positive cases as well as a sample of microscopically negative cases were included for molecular analysis by polymerase chain reaction (PCR).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n 254 microscopically negative and 193 microscopically positive malaria suspects were included. Of the 193 malaria positive cases, 46.1% [95% confidence interval (CI) 38.9\u201353.4] (89\/193) were\n P. falciparum<\/jats:italic>\n infection, 52.3% (95% CI 45.0\u201359.5) (101\/193) were\n P. vivax<\/jats:italic>\n infection, and 1.6% (3\/193) had mixed infection of\n P. falciparum<\/jats:italic>\n and\n P. vivax<\/jats:italic>\n . Of the microscopically positive cases of\n P. falciparum<\/jats:italic>\n , 3.4% (3\/89) were\n P. vivax<\/jats:italic>\n and 11.2% (10\/89) were mixed infections with\n P. falciparum<\/jats:italic>\n and\n P. vivax<\/jats:italic>\n and a single case was negative molecularly. Similarly, of the microscopically positive\n P. vivax<\/jats:italic>\n cases, 5.9% (6\/101) were\n P. falciparum<\/jats:italic>\n and 1% (1\/101) was mixed infection. Single case was negative by molecular technique. Of the 254 microscopically negative cases, 0.8% were tested positive for\n P. falciparum<\/jats:italic>\n and 2% for\n P. vivax<\/jats:italic>\n by PCR\n .<\/jats:italic>\n Considering molecular technique as a reference, the sensitivity of microscopy for detecting\n P. falciparum<\/jats:italic>\n was 89.2% and for\n P. vivax<\/jats:italic>\n , it was 91.2%. The specificity of microscopy for detecting\n P. falciparum<\/jats:italic>\n was 96.1% and for\n P. vivax<\/jats:italic>\n , it was 97.7%. However, the sensitivity of microscopy in detecting mixed infection of\n P. falciparum<\/jats:italic>\n and\n P. vivax<\/jats:italic>\n was low (8.3%).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n There were cases left untreated or inappropriately treated due to the species misidentification. Therefore, to minimize this problem, the gaps in the microscopic-based malaria diagnosis should be identified. It is recommended to regularly monitor the competency of malaria microscopists in the study area to improve species identification and diagnosis accuracy.<\/jats:p>\n <\/jats:sec>","DOI":"10.1186\/s12936-023-04711-2","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T10:50:21Z","timestamp":1694688621000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Malaria misdiagnosis in the routine health system in Arba Minch area district in southwest Ethiopia: an implication for malaria control and elimination"],"prefix":"10.1186","volume":"22","author":[{"given":"Engida","family":"Yigezu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biniam","family":"Wondale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Abebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girum","family":"Tamiru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigatu","family":"Eligo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernt","family":"Lindtj\u00f8rn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Endalamaw","family":"Gadisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fitsum Girma","family":"Tadesse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fekadu","family":"Massebo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,9,14]]},"reference":[{"key":"4711_CR1","volume-title":"World malaria report 2021","author":"WHO","year":"2021","unstructured":"WHO. 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