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The present study aimed to standardize <jats:italic>Plasmodium<\/jats:italic> sp. DNA extraction from histological slices of spleen and diagnosis using real-time qPCR.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>This study performed a microtomy of a paraffin-embedded spleen as a positive control for <jats:italic>P. vivax<\/jats:italic> from a patient who had been previously diagnosed with the parasite. The sample was deparaffinized with xylol and ethanol, then DNA extraction was performed with two commercial kits. qPCR was carried out with the <jats:italic>Taqman<\/jats:italic> system for detection of <jats:italic>Plasmodium<\/jats:italic> sp. and was made species-specific using <jats:italic>PvmtCOX1<\/jats:italic> gene. From 2015 to 2019, 200 spleen samples were obtained from trauma patients subjected to splenectomy in Manaus, Amazonas. All the samples were tested for cell-free human DNA (cfDNA).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The deparaffinization and the <jats:italic>Plasmodium vivax<\/jats:italic> DNA extraction method was successfully standardized, and the control sample was positive for <jats:italic>P. vivax.<\/jats:italic> Of the 200 samples, all qPCRs were negative, but they were positive for human PCR.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Paraffinization is practical and efficient for the preservation of samples, but the formation of bonds between proteins and DNA makes extraction difficult. Despite this, in this study, it was possible to standardize a method of DNA extraction for detecting <jats:italic>P. vivax.<\/jats:italic><\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12936-023-04764-3","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T15:03:55Z","timestamp":1701097435000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Standardization of DNA extraction from paraffinized spleen samples: molecular diagnosis of human malaria"],"prefix":"10.1186","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9649-6618","authenticated-orcid":false,"given":"Raimunda Sandra","family":"Pacheco Souza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7849-6112","authenticated-orcid":false,"given":"Monique F.","family":"dos Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9657-939X","authenticated-orcid":false,"given":"Luiz Carlos","family":"de Lima Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela C.","family":"Morais","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Kassio S.","family":"Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laila Rowena","family":"Albuquerque Barbosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8155-6005","authenticated-orcid":false,"given":"Gisely Cardoso","family":"de Melo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3374-9985","authenticated-orcid":false,"given":"Marcus Vinicius Guimaraes","family":"de Lacerda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"4764_CR1","volume-title":"World malaria report 2015","author":"WHO","year":"2016","unstructured":"WHO. 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