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Data on the frequency of\n                      <jats:italic>hrp2\/hrp3<\/jats:italic>\n                      deleted parasites are rarely available and it has become necessary to investigate the issue in Burkina Faso.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Plasmodium falciparum-<\/jats:italic>\n                      positive dried blood spots were collected during a cross-sectional household survey of the malaria asymptomatic children from Orodara, Gaoua, and Banfora. Amplicons from the target regions (exon 2 of\n                      <jats:italic>hrp2<\/jats:italic>\n                      and\n                      <jats:italic>hrp3<\/jats:italic>\n                      genes) were generated using multiplexed nested PCR and sequenced according to Illumina\u2019s MiSeq protocol.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 251 microscopically positive parasite isolates were sequenced to detect\n                      <jats:italic>hrp2<\/jats:italic>\n                      and\n                      <jats:italic>hrp3<\/jats:italic>\n                      gene deletions. The proportion of RDTs negative cases among microscopy positive slides was 12.7% (32\/251). The highest prevalence of negative RDTs was found in Orodara 14.3% (5\/35), followed by Gaoua 13.1%(24\/183), and Banfora 9.1% (3\/33). The study found that 95.6% of the parasite isolates were wild type\n                      <jats:italic>hrp2\/ hrp3<\/jats:italic>\n                      while 4.4% (11\/251) had a single\n                      <jats:italic>hrp2<\/jats:italic>\n                      deletion. Of the 11\n                      <jats:italic>hrp2<\/jats:italic>\n                      deletion samples, 2 samples were RDT negative (mean parasitaemia was 83 parasites\/ \u03bcL) while 9 samples were RDT positive with a mean parasitaemia of 520 parasites \/\u03bcL (CI95%: 192\u20131239). The highest frequency of\n                      <jats:italic>hrp2<\/jats:italic>\n                      deletion 4\/35 (11.4%) was found in Orodara, while it was similar in the other two sites (&lt;\u20093.5%). No single deletion of the\n                      <jats:italic>hrp3<\/jats:italic>\n                      or dual deletion\n                      <jats:italic>hrp2\/3<\/jats:italic>\n                      gene was detected in this study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These results demonstrate that\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      isolates lacking\n                      <jats:italic>hrp2<\/jats:italic>\n                      genes are present in 4.4% of samples obtained from the asymptomatic children population in three sites in Burkina Faso. These parasites are circulating and causing malaria, but they are also still detectable by HRP2-based RTDs due to the presence of the intact\n                      <jats:italic>pfhrp3<\/jats:italic>\n                      gene.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-023-04796-9","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T19:23:40Z","timestamp":1701199420000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Assessing the histidine-rich protein 2\/3 gene deletion in Plasmodium falciparum isolates from Burkina Faso"],"prefix":"10.1186","volume":"22","author":[{"given":"Casimire Wendlamita","family":"Tarama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harouna","family":"Sor\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mafama","family":"Siribi\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siaka","family":"D\u00e9b\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9n\u00e9","family":"Kinda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendyam G\u00e9rard","family":"Nonkani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farida","family":"Tiendrebeogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winnie","family":"Bantango","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kassoum","family":"Yira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther Y\u00e9ri","family":"Hien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moussa Wandaogo","family":"Guelb\u00e9ogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Traor\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"M\u00e9nard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adama","family":"Gansan\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,11,29]]},"reference":[{"key":"4796_CR1","unstructured":"Annuaire Statistique 2021. 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