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Understanding its developmental biology is crucial for interrupting malaria transmission.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study was conducted in Tapachula, Chiapas, a city located in southeastern Mexico. Oviposition and fecundity patterns were determined in nulliparous and parous females, individually and in groups, under insectary and semi-field conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Regardless of whether they were grouped or individual, insectary-reared or field-collected, mosquito females exhibited a primary oviposition peak between 18:00 and 20:00, followed by a gradual decline until midnight. While nulliparous females from the insectary ceased oviposition after 24:00, a small percentage of parous females continued laying eggs throughout the night, until shortly before dawn (between 24:00 and 06:00). Parous females laid more eggs than nulliparous females<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Nulliparous and parous females of\n                      <jats:italic>An. pseudopunctipennis<\/jats:italic>\n                      exhibited a major oviposition peak between 18:00 and 20:00, a few minutes after sunset. Oviposition activity persisted, albeit at a lower rate, until 24:00. A small subset of parous females continued ovipositing until 6:00. Parous females were observed laying more eggs than nulliparous females. Based on the high activity of\n                      <jats:italic>An. pseudopunctipennis<\/jats:italic>\n                      , from dusk until midnight, chemical control with pyrethroid insecticides applied by ultra-low volume (ULV) or by thermal fogging is proposed in breeding sites during this period, in areas near localities with active malaria transmission.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12936-026-05862-8","type":"journal-article","created":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T17:04:54Z","timestamp":1774199094000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Daily pattern of oviposition and fecundity in Anopheles pseudopunctipennis Theobald, the main vector of malaria in Mexico"],"prefix":"10.1186","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3859-2403","authenticated-orcid":false,"given":"Cuauht\u00e9moc","family":"Villarreal-Trevi\u00f1o","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2347-5581","authenticated-orcid":false,"given":"Lilia","family":"Gonz\u00e1lez-Cer\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9352-5843","authenticated-orcid":false,"given":"Jana Celina","family":"R\u00edos-Delgado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0780-1527","authenticated-orcid":false,"given":"Rosa Patricia","family":"Penilla-Navarro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,3,22]]},"reference":[{"key":"5862_CR1","unstructured":"Villarreal-Trevi\u00f1o C, Arredondo-Jim\u00e9nez JI, Rodr\u00edguez MH. 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