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However, the effects of radial tensile stimuli on differentiation of MSCs into fibrocartilage remain unclear.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>It was found that induced bone marrow mesenchymal stem cells (BMSCs) were not only similar to TMJ disc cells in morphology, but also could synthesize type I collagen (Col I), a small amount of type II collagen (Col II) and glycosaminoglycans (GAGs). The synthesis of Col I significantly increased while that of Col II gradually decreased with increasing tensile strength. The ratio of Col I to Col II was 1.8 to 1 and 2 to 1 in the 10% and 15% stretching groups, respectively. The gene expression of Col I and GAGs was significantly upregulated, whereas that of Col II was downregulated. However, the higher tensile stimulation (15%) promoted the synthesis of \u03b1-smooth muscle actin (\u03b1-SMA). Too much \u03b1-SMA is not conducive to constructing engineered tissue.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Therefore, the 10% radial tensile stimulus was the optimal strength for inducing the BMSCs to differentiate into fibrochondrocytes of the temporomandibular joint (TMJ) disc. This work provided a novel approach for inducing BMSCs to differentiate into fibrochondrocytes.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12938-020-0751-1","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T13:21:36Z","timestamp":1583500896000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effects of dynamic radial tensile stress on fibrocartilage differentiation of bone marrow mesenchymal stem cells"],"prefix":"10.1186","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8758-1773","authenticated-orcid":false,"given":"Xuelian","family":"Su","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jizeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjie","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,5]]},"reference":[{"issue":"6","key":"751_CR1","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1007\/s11914-016-0327-y","volume":"14","author":"A Aryaei","year":"2016","unstructured":"Aryaei A, Vapniarsky N, Hu JC, Athanasiou KA. 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