{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:50:47Z","timestamp":1793235047451,"version":"4.5.0"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BioMed Eng OnLine"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Psoriasis is a common chronic recurrent inflammatory skin disease. The pathogenesis of psoriasis, such as other autoimmune diseases, is still unclear, which brings great difficulties to the treatment. This study aimed to investigate the role of bromine domain protein 4 (BRD4) in affecting the psoriatic keratinocytes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Imiquimod-induced psoriasis mice model and TNF-\u03b1 or IL-17A induced HaCAT cells, an experimental model in vitro for psoriasis, were constructed. The pathological skin changes at the back of mice were observed by hematoxylin and eosin (H&amp;E) assay and evaluated by psoriasis area and severity index (PASI). KI67 expression and keratinocyte apoptosis at the skin tissues were, respectively, detected by Immunohistochemical analysis and TUNEL assay. The inflammatory factors in mice serum and culture supernatant were determined by ELISA assay. The related proteins expression of proliferation, apoptosis and MAPK pathway were detected by Western blot analysis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>BRD4 expression was upregulated in injured skin on the back of imiquimod-induced mice and (+)-JQ1 relieved the skin injury by suppressing the inflammation and promoting apoptosis of keratinocytes. Consistently, BRD4 expression was also increased in TNF-\u03b1 or IL-17A induced HaCAT cells. (+)-JQ1 suppressed the viability and inflammation, and promoted apoptosis of TNF-\u03b1 or IL-17A induced HaCAT cells. In addition, the MAPK signaling pathway was inhibited by (+)-JQ1 whether in mice or HaCAT cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Inhibition of BRD4 inhibited proliferation and inflammation and promoted apoptosis of psoriatic keratinocytes.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12938-021-00943-y","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T10:05:04Z","timestamp":1634810704000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Inhibition of BRD4 inhibits proliferation and promotes apoptosis of psoriatic keratinocytes"],"prefix":"10.1186","volume":"20","author":[{"given":"Xiaohui","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"issue":"4","key":"943_CR1","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1016\/j.jaad.2012.05.016","volume":"67","author":"NN Mehta","year":"2012","unstructured":"NN Mehta P Krishnamoorthy Y Yu O Khan A Raper A Voorhees Van AB Troxel JM Gelfand 2012 The impact of psoriasis on 10-year Framingham risk J Am Acad Dermatol 67 4 796 798","journal-title":"J Am Acad Dermatol"},{"issue":"2","key":"943_CR2","first-page":"01","volume":"10","author":"U Praveenkumar","year":"2016","unstructured":"U Praveenkumar S Ganguly L Ray SK Nanda S Kuruvila 2016 Prevalence of metabolic syndrome in psoriasis patients and its relation to disease duration: a hospital based case-control study J Clin Diagn Res 10 2 01 05","journal-title":"J Clin Diagn Res"},{"issue":"8","key":"943_CR3","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1111\/1346-8138.12465","volume":"41","author":"JL Sanchez-Carazo","year":"2014","unstructured":"JL Sanchez-Carazo JL L\u00f3pez-Estebaranz C Guisado 2014 Comorbidities and health-related quality of life in Spanish patients with moderate to severe psoriasis: a cross-sectional study (Arizona study) J Dermatol 41 8 673 678","journal-title":"J Dermatol"},{"issue":"7130","key":"943_CR4","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1038\/nature05663","volume":"445","author":"MA Lowes","year":"2007","unstructured":"MA Lowes AM Bowcock JG Krueger 2007 Pathogenesis and therapy of psoriasis Nature 445 7130 866 873","journal-title":"Nature"},{"issue":"2","key":"943_CR5","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1097\/01.ASW.0000550592.08674.43","volume":"32","author":"A Brandon","year":"2019","unstructured":"A Brandon A Mufti R Gary Sibbald 2019 Diagnosis and management of cutaneous psoriasis: a review Adv Skin Wound Care 32 2 58 69","journal-title":"Adv Skin Wound Care"},{"issue":"8","key":"943_CR6","doi-asserted-by":"publisher","first-page":"5501","DOI":"10.18632\/oncotarget.3551","volume":"6","author":"LL Fu","year":"2015","unstructured":"LL Fu M Tian X Li JJ Li J Huang L Ouyang Y Zhang B Liu 2015 Inhibition of BET bromodomains as a therapeutic strategy for cancer drug discovery Oncotarget 6 8 5501 5516","journal-title":"Oncotarget"},{"issue":"3","key":"943_CR7","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1158\/2159-8290.CD-13-0018","volume":"3","author":"RW Schnepp","year":"2013","unstructured":"RW Schnepp JM Maris 2013 Targeting MYCN: a good BET for improving neuroblastoma therapy? 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