{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:39:30Z","timestamp":1792787970659,"version":"4.5.0"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"name":"investigator-initiated grant from Pfizer","award":["W1172148"],"award-info":[{"award-number":["W1172148"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Clin Microbiol Antimicrob"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s12941-017-0200-6","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T02:13:10Z","timestamp":1491358390000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Molecular characterization of penicillin non-susceptible Streptococcus pneumoniae isolated before and after pneumococcal conjugate vaccine implementation in Casablanca, Morocco"],"prefix":"10.1186","volume":"16","author":[{"given":"Idrissa","family":"Diawara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abouddihaj","family":"Barguigua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid","family":"Katfy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaotar","family":"Nayme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houria","family":"Belabbes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Timinouni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid","family":"Zerouali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naima","family":"Elmdaghri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,4,4]]},"reference":[{"issue":"32","key":"200_CR1","doi-asserted-by":"crossref","first-page":"4717","DOI":"10.1016\/j.vaccine.2012.04.093","volume":"30","author":"Organisation Mondiale de la Sant\u00e9","year":"2012","unstructured":"Organisation Mondiale de la Sant\u00e9. Pneumococcal vaccines WHO position paper-2012-recommendations. Vaccine. 2012;30(32):4717\u20138.","journal-title":"Vaccine"},{"issue":"12","key":"200_CR2","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1086\/340400","volume":"34","author":"PC Appelbaum","year":"2002","unstructured":"Appelbaum PC. Resistance among Streptococcus pneumoniae: implications for drug selection. Clin Infect Dis. 2002;34(12):1613\u201320.","journal-title":"Clin Infect Dis"},{"issue":"17","key":"200_CR3","doi-asserted-by":"crossref","first-page":"2728","DOI":"10.1016\/j.vaccine.2012.01.091","volume":"30","author":"JH Song","year":"2012","unstructured":"Song JH, Dagan R, Klugman KP, Fritzell B. The relationship between pneumococcal serotypes and antibiotic resistance. Vaccine. 2012;30(17):2728\u201337.","journal-title":"Vaccine"},{"issue":"4","key":"200_CR4","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s10096-016-2582-3","volume":"35","author":"X Zhou","year":"2016","unstructured":"Zhou X, Liu J, Zhang Z, Liu Y, Wang Y, Liu Y. Molecular characteristics of penicillin-binding protein 2b, 2x and 1a sequences in Streptococcus pneumoniae isolates causing invasive diseases among children in Northeast China. Eur J Clin Microbiol Infect Dis. 2016;35(4):633\u201345.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"200_CR5","first-page":"245","volume":"2","author":"DB Hansman","year":"1967","unstructured":"Hansman DB. M. M. : a resistant pneumococcus. Lancet. 1967;2:245\u2013245.","journal-title":"Lancet"},{"key":"200_CR6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1146\/annurev.mi.45.100191.000345","volume":"45","author":"JM Ghuysen","year":"1991","unstructured":"Ghuysen JM. Serine beta-lactamases and penicillin-binding proteins. Annu Rev Microbiol. 1991;45:37\u201367.","journal-title":"Annu Rev Microbiol"},{"issue":"4","key":"200_CR7","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1128\/AAC.30.4.553","volume":"30","author":"R Hakenbeck","year":"1986","unstructured":"Hakenbeck R, Ellerbrok H, Briese T, Handwerger S, Tomasz A. Penicillin-binding proteins of penicillin-susceptible and -resistant pneumococci: immunological relatedness of altered proteins and changes in peptides carrying the beta-lactam binding site. Antimicrob Agents Chemother. 1986;30(4):553\u20138.","journal-title":"Antimicrob Agents Chemother"},{"issue":"5","key":"200_CR8","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1128\/AAC.46.5.1273-1280.2002","volume":"46","author":"K Nagai","year":"2002","unstructured":"Nagai K, Davies TA, Jacobs MR, Appelbaum PC. Effects of amino acid alterations in penicillin-binding proteins (PBPs) 1a, 2b, and 2x on PBP affinities of penicillin, ampicillin, amoxicillin, cefditoren, cefuroxime, cefprozil, and cefaclor in 18 clinical isolates of penicillin-susceptible, -intermediate, and -resistant Pneumococci. Antimicrob Agents Chemother. 2002;46(5):1273\u201380.","journal-title":"Antimicrob Agents Chemother"},{"issue":"3","key":"200_CR9","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/j.1574-6968.1995.tb07433.x","volume":"126","author":"VA Barcus","year":"1995","unstructured":"Barcus VA, Ghanekar K, Yeo M, Coffey TJ, Dowson CG. Genetics of high level penicillin resistance in clinical isolates of Streptococcus pneumoniae. FEMS Microbiol Lett. 1995;126(3):299\u2013303.","journal-title":"FEMS Microbiol Lett"},{"issue":"2","key":"200_CR10","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1093\/jac\/dki442","volume":"57","author":"F Cafini","year":"2006","unstructured":"Cafini F, del Campo R, Alou L, Sevillano D, Morosini MI, Baquero F, Prieto J. Alterations of the penicillin-binding proteins and murM alleles of clinical Streptococcus pneumoniae isolates with high-level resistance to amoxicillin in Spain. J Antimicrob Chemother. 2006;57(2):224\u20139.","journal-title":"J Antimicrob Chemother"},{"key":"200_CR11","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ijid.2015.09.019","volume":"40","author":"I Diawara","year":"2015","unstructured":"Diawara I, Zerouali K, Katfy K, Zaki B, Belabbes H, Najib J, Elmdaghri N. Invasive pneumococcal disease among children younger than 5\u00a0years of age before and after introduction of pneumococcal conjugate vaccine in Casablanca. Morocco. Int J Infect Dis. 2015;40:95\u2013101.","journal-title":"Morocco. Int J Infect Dis"},{"issue":"3","key":"200_CR12","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1093\/infdis\/jis703","volume":"207","author":"KL Wyres","year":"2013","unstructured":"Wyres KL, Lambertsen LM, Croucher NJ, McGee L, von Gottberg A, Linares J, Jacobs MR, Kristinsson KG, Beall BW, Klugman KP, et al. Pneumococcal capsular switching: a historical perspective. J Infect Dis. 2013;207(3):439\u201349.","journal-title":"J Infect Dis"},{"issue":"12","key":"200_CR13","doi-asserted-by":"crossref","first-page":"4361","DOI":"10.1128\/JCM.38.12.4361-4366.2000","volume":"38","author":"D Bogaert","year":"2000","unstructured":"Bogaert D, Syrogiannopoulos GA, Grivea IN, de Groot R, Beratis NG, Hermans PW. Molecular epidemiology of penicillin-nonsusceptible Streptococcus pneumoniae among children in Greece. J Clin Microbiol. 2000;38(12):4361\u20136.","journal-title":"J Clin Microbiol"},{"key":"200_CR14","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.meegid.2016.03.003","volume":"40","author":"I Diawara","year":"2016","unstructured":"Diawara I, Zerouali K, Katfy K, Barguigua A, Belabbes H, Timinouni M, Elmdaghri N. Phenotypic and genotypic characterization of Streptococcus pneumoniae resistant to macrolide in Casablanca, Morocco. Infect Genet Evol. 2016;40:200\u20134.","journal-title":"Infect Genet Evol"},{"issue":"1","key":"200_CR15","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1086\/315194","volume":"181","author":"G Gherardi","year":"2000","unstructured":"Gherardi G, Whitney CG, Facklam RR, Beall B. Major related sets of antibiotic-resistant Pneumococci in the United States as determined by pulsed-field gel electrophoresis and pbp1a-pbp2b-pbp2x-dhf restriction profiles. J Infect Dis. 2000;181(1):216\u201329.","journal-title":"J Infect Dis"},{"issue":"1","key":"200_CR16","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1093\/jac\/dkh271","volume":"54","author":"DC Bean","year":"2004","unstructured":"Bean DC, Ikram RB, Klena JD. Molecular characterization of penicillin non-susceptible Streptococcus pneumoniae in Christchurch. New Zealand. J Antimicrob Chemother. 2004;54(1):122\u20139.","journal-title":"New Zealand. J Antimicrob Chemother"},{"issue":"7","key":"200_CR17","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1128\/JCM.36.7.2115-2116.1998","volume":"36","author":"JA Elliott","year":"1998","unstructured":"Elliott JA, Farmer KD, Facklam RR. Sudden increase in isolation of group B streptococci, serotype V, is not due to emergence of a new pulsed-field gel electrophoresis type. J Clin Microbiol. 1998;36(7):2115\u20136.","journal-title":"J Clin Microbiol"},{"issue":"5","key":"200_CR18","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1111\/j.1469-0691.2010.03182.x","volume":"16","author":"J Linares","year":"2010","unstructured":"Linares J, Ardanuy C, Pallares R, Fenoll A. Changes in antimicrobial resistance, serotypes and genotypes in Streptococcus pneumoniae over a 30-year period. Clin Microbiol Infect. 2010;16(5):402\u201310.","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"200_CR19","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1128\/CMR.00018-12","volume":"25","author":"TQ Tan","year":"2012","unstructured":"Tan TQ. Pediatric invasive pneumococcal disease in the United States in the era of pneumococcal conjugate vaccines. Clin Microbiol Rev. 2012;25(3):409\u201319.","journal-title":"Clin Microbiol Rev"},{"issue":"12","key":"200_CR20","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1139\/cjm-2013-0614","volume":"59","author":"WH Demczuk","year":"2013","unstructured":"Demczuk WH, Martin I, Griffith A, Lefebvre B, McGeer A, Lovgren M, Tyrrell GJ, Desai S, Sherrard L, Adam H, et al. Serotype distribution of invasive Streptococcus pneumoniae in Canada after the introduction of the 13-valent pneumococcal conjugate vaccine, 2010\u20132012. Can J Microbiol. 2013;59(12):778\u201388.","journal-title":"Can J Microbiol"},{"issue":"20","key":"200_CR21","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1056\/NEJMoa1401914","volume":"371","author":"A Gottberg von","year":"2014","unstructured":"von Gottberg A, de Gouveia L, Tempia S, Quan V, Meiring S, von Mollendorf C, Madhi SA, Zell ER, Verani JR, O\u2019Brien KL, et al. Effects of vaccination on invasive pneumococcal disease in South Africa. N Engl J Med. 2014;371(20):1889\u201399.","journal-title":"N Engl J Med"},{"issue":"7","key":"200_CR22","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.3201\/eid1907.121830","volume":"19","author":"SS Richter","year":"2013","unstructured":"Richter SS, Heilmann KP, Dohrn CL, Riahi F, Diekema DJ, Doern GV. Pneumococcal serotypes before and after introduction of conjugate vaccines, United States, 1999-2011(1.). Emerg Infect Dis. 2013;19(7):1074\u201383.","journal-title":"Emerg Infect Dis"},{"issue":"51","key":"200_CR23","doi-asserted-by":"crossref","first-page":"6150","DOI":"10.1016\/j.vaccine.2013.05.042","volume":"31","author":"SR Santos dos","year":"2013","unstructured":"dos Santos SR, Passadore LF, Takagi EH, Fujii CM, Yoshioka CR, Gilio AE, Martinez MB. Serotype distribution of Streptococcus pneumoniae isolated from patients with invasive pneumococcal disease in Brazil before and after ten-pneumococcal conjugate vaccine implementation. Vaccine. 2013;31(51):6150\u20134.","journal-title":"Vaccine"},{"issue":"8","key":"200_CR24","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1089\/mdr.2015.0261","volume":"22","author":"EY Liu","year":"2016","unstructured":"Liu EY, Chang JC, Lin JC, Chang FY, Fung CP. Important Mutations contributing to high-level penicillin resistance in Taiwan19F-14, Taiwan23F-15, and Spain23F-1 of Streptococcus pneumoniae Isolated from Taiwan. Microb Drug Resist. 2016;22(8):646\u201354.","journal-title":"Microb Drug Resist"},{"issue":"12","key":"200_CR25","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S1473-3099(08)70281-0","volume":"8","author":"R Dagan","year":"2008","unstructured":"Dagan R, Klugman KP. Impact of conjugate pneumococcal vaccines on antibiotic resistance. Lancet Infect Dis. 2008;8(12):785\u201395.","journal-title":"Lancet Infect Dis"},{"issue":"2","key":"200_CR26","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jinf.2004.02.014","volume":"49","author":"EK Yee","year":"2004","unstructured":"Yee EK, Melkerson-Watson LJ, Bloch CA, Pierson CL, Blackwood RA. Genomic analysis of penicillin-resistant Streptococcus pneumoniae in Southeastern Michigan. J Infect. 2004;49(2):126\u201335.","journal-title":"J Infect"},{"issue":"2","key":"200_CR27","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1089\/mdr.1999.5.91","volume":"5","author":"R Hakenbeck","year":"1999","unstructured":"Hakenbeck R, Kaminski K, Konig A, van der Linden M, Paik J, Reichmann P, Zahner D. Penicillin-binding proteins in beta-lactam-resistant Streptococcus pneumoniae. Microb Drug Resist. 1999;5(2):91\u20139.","journal-title":"Microb Drug Resist"}],"container-title":["Annals of Clinical Microbiology and Antimicrobials"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12941-017-0200-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,4]],"date-time":"2020-10-04T19:19:43Z","timestamp":1601839183000},"score":1,"resource":{"primary":{"URL":"http:\/\/ann-clinmicrob.biomedcentral.com\/articles\/10.1186\/s12941-017-0200-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,4]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["200"],"URL":"https:\/\/doi.org\/10.1186\/s12941-017-0200-6","relation":{},"ISSN":["1476-0711"],"issn-type":[{"value":"1476-0711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,4]]},"article-number":"23"}}